RESUMO
Natural peloids from sulfurous thermal springs are largely used in cosmetic and pelotherapy for the treatment of different dermatological conditions, including skin aging, dermatitis, and other eczemas. The beneficial effects are correlated to mineralogical and other thermal properties, as well as to the presence of natural substances with specific antioxidant activity. Few data are available for the comparison between natural peloids and synthetic (i.e., artificially maturated) muds. In this context, the natural substances and antioxidant activity of natural white mud (WM) and dark mud (DM) peloids from the sulfurous thermal spring pool Bagnaccio (Viterbo, Italy) have been studied in detail to evaluate possible relationships between physicochemical properties and therapeutic effect. A large panel of natural substances in WM and DM were characterized for the first time by ³¹P-nuclear magnetic resonance and gas chromatography associated to mass spectrometry analysis. Polar fractions of WM and DM peloids were characterized by the presence of several bioactive natural compounds, showing high antioxidant activity and DNA protective effect, as evaluated by 2,2-diphenyl-1-picrylhydrazyl assay, and hydrogen peroxideinduced DNA breakage in the alkaline comet assay. The antioxidant activity and DNA protective effect could be attributed to radical scavenging rather than a modulatory effect on the induced DNA repair, and are of order of intensity higher than that reported for synthetic muds.
Assuntos
Alcaloides/farmacologia , Sequestradores de Radicais Livres/farmacologia , Hidrocarbonetos Aromáticos/farmacologia , Fenóis/farmacologia , Terpenos/farmacologia , Alcaloides/química , Alcaloides/isolamento & purificação , Alcanos/química , Alcanos/isolamento & purificação , Alcanos/farmacologia , Alcenos/química , Alcenos/isolamento & purificação , Alcenos/farmacologia , Animais , Compostos de Bifenilo/antagonistas & inibidores , Compostos de Bifenilo/química , Células CHO , Linhagem Celular Tumoral , Sobrevivência Celular/efeitos dos fármacos , Ensaio Cometa , Cricetulus , DNA/efeitos dos fármacos , Sequestradores de Radicais Livres/química , Sequestradores de Radicais Livres/isolamento & purificação , Fontes Termais , Humanos , Hidrocarbonetos Aromáticos/química , Hidrocarbonetos Aromáticos/isolamento & purificação , Peróxido de Hidrogênio/antagonistas & inibidores , Peróxido de Hidrogênio/química , Itália , Espectroscopia de Ressonância Magnética , Camundongos , Peloterapia , Fenóis/química , Fenóis/isolamento & purificação , Picratos/antagonistas & inibidores , Picratos/química , Terpenos/química , Terpenos/isolamento & purificaçãoRESUMO
Catechol derivatives with lipophilic properties have been selectively synthesized by tyrosinase in high yield avoiding long and tedious protection/deprotection steps usually required in traditional procedures. The synthesis was effective also with immobilized tyrosinase able to perform for more runs. The novel catechols were evaluated against influenza A virus, that continue to represent a severe threat worldwide. A significant antiviral activity was observed in derivatives characterized by antioxidant activity and long carbon alkyl side-chains, suggesting the possibility of a new inhibition mechanism based on both redox and lipophilic properties.
