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1.
Zootaxa ; 5128(4): 538-546, 2022 Apr 22.
Artigo em Inglês | MEDLINE | ID: mdl-36101159

RESUMO

Hydraena (Hydraenopsis) bahiana sp. nov. is described from Bahia State, Northeast Brazil, based on three specimens collected in pools at the headwater of a stream, 1,705 m. asl. The new species belongs to the Hydraena scintillabella subgroup of the leechi group. Description and diagnostic characters are offered; habitus photographs and line drawings of both male and female genitalia are provided. Habitat and distributional data of the new species are also included.


Assuntos
Briófitas , Besouros , Animais , Brasil , Ecossistema , Feminino , Masculino , Rios
2.
Zootaxa ; 4966(1): 6168, 2021 Apr 30.
Artigo em Inglês | MEDLINE | ID: mdl-34186633

RESUMO

Undescribed morphological features of Adelphydraena amazonica Perkins Ribera, 2020 are provided based on material collected in the type locality (Amazonas State, northern Brazil). Aedeagus, male sternite X, last female abdominal segments and spermatheca are illustrated and described for the first time. Habitat information on the type locality is also included. New records on two Brazilian Hydraenidae species are provided.


Assuntos
Besouros/anatomia & histologia , Besouros/classificação , Animais , Brasil , Ecossistema , Feminino , Masculino
3.
Rev. biol. trop ; 69(2)jun. 2021.
Artigo em Inglês | LILACS, SaludCR | ID: biblio-1387659

RESUMO

Abstract Introduction: Despite extensive science-based conservation policy recommendations, with fewer than 20 individuals remaining, the vaquita (Phocoena sinus) -endemic to the Gulf of California- is the world's most endangered marine mammal due to incidental catch in fishing nets and whether it can recover is unclear. Objective: Assess expectations for vaquita over the next two decades. Methods: We identified factors affecting the vaquita, constructed life tables, derived demographic parameters for different scenarios and conducted a population viability analysis using stochastic age-structured matrix Leslie models. Results: Analytical results indicate that the vaquita net growth rate is particularly sensitive to juvenile survival. We find that intensive, ongoing bycatch in gillnets used to poach totoaba (Totoaba macdonaldi) over the past decade brought the vaquita population to its current critically low size. Currently this seems to be exacerbated by demographic stochasticity and a potential Allee effect. Conclusions: If totoaba poaching is eliminated immediately, demographically, vaquita can recover; its long-term survival will depend on its uncertain genetic status, although a recent study found encouraging results in this regard.


Resumen Introducción: Pese a las acciones de conservación basadas en la ciencia y las políticas recomendadas, con menos de 20 individuos sobrevivientes, la vaquita (Phocoena sinus) -endémica del Golfo de California- es el mamífero marino más amenazado del mundo debido a su muerte incidental en redes de pesca; una pregunta relevante es si su población se puede recuperar. Objetivo: Evaluar las expectativas para la vaquita marina durante los próximos 20 años. Métodos: Identificamos los factores que afectan a la vaquita marina, construimos tablas de vida, derivamos parámetros demográficos para diferentes escenarios y realizamos un análisis de viabilidad poblacional utilizando matrices estocásticas de Leslie, estructuradas por edad. Resultados: La tasa de crecimiento neto de la vaquita es muy sensible a la supervivencia de los juveniles. Encontramos que la captura incidental intensiva y continua en redes de enmalle para la pesca furtiva de totoaba (Totoaba macdonaldi) durante la última década llevó a la población de vaquitas a su actual estado crítico. Esto parece agravarse por la estocasticidad demográfica y un potencial efecto Allee. Conclusiones: Si la pesca furtiva de totoaba se elimina de inmediato, demográficamente la vaquita puede recuperarse; su supervivencia a largo plazo dependerá de su incierto estatus genético, aunque los resultados de un estudio reciente son alentadores en este sentido.


Assuntos
Animais , Espécies em Perigo de Extinção , Phocoena , Indústria Pesqueira , California
4.
Int J Cardiol ; 321: 48-53, 2020 Dec 15.
Artigo em Inglês | MEDLINE | ID: mdl-32810542

RESUMO

BACKGROUND: RENASCENT is a prospective, multi-center first-in-human clinical study to evaluate the clinical performance of the novel sirolimus-eluting 150-µm strut thickness FORTITUDE® BRS for percutaneous coronary intervention of single de novo coronary lesions. METHODS: FORTITUDE® BRS was tested in a prospective study in Italy and Colombia. Study objectives were in-scaffold angiographic late lumen loss (LLL) measured by quantitative coronary angiography and target vessel failure (TVF) defined as the composite rate of cardiac death, target vessel myocardial infarction or ischemia driven target lesion revascularization (TLR) at 9- and 24-months with clinical results up to 36-months. RESULTS: A total of 63 patients were enrolled. All patients underwent lesion pre-dilatation and 22 patients (34.9%) underwent post-dilatation. Clinical device and procedural success was 98.4% (62/63 patients) and 96.8% (61/63 patients) respectively. At 9-months, TVF occurred in 3/61 (4.9%) of the patients including 2 peri-procedural MI and one ischemia-driven TLR. Between 9- to 24-months, ischemia-driven TLR occurred in 3 additional patients (4.9%) including 1 patient who presented with very late ST after stopping all medications. There were no further TVF between 24- and 36-months. CONCLUSIONS: In this multi-center prospective study, the FORTITUDE® BRS was shown to be safe and effective in the treatment of single coronary lesions with low levels of TVF and LLL at 9- and 24-months. It was shown to be clinically safe upto 36-months follow-up.


Assuntos
Fármacos Cardiovasculares , Doença da Artéria Coronariana , Reestenose Coronária , Stents Farmacológicos , Intervenção Coronária Percutânea , Implantes Absorvíveis , Fármacos Cardiovasculares/uso terapêutico , Colômbia , Angiografia Coronária , Doença da Artéria Coronariana/diagnóstico por imagem , Doença da Artéria Coronariana/tratamento farmacológico , Doença da Artéria Coronariana/cirurgia , Reestenose Coronária/tratamento farmacológico , Humanos , Itália , Peso Molecular , Estudos Prospectivos , Desenho de Prótese , Sirolimo , Tomografia de Coerência Óptica , Resultado do Tratamento
5.
Zootaxa ; 4750(3): zootaxa.4750.3.5, 2020 Mar 12.
Artigo em Inglês | MEDLINE | ID: mdl-32230460

RESUMO

Hydraena (Hydraenopsis) josefinae sp. nov. and H. (H.) pernambucana sp. nov. are described respectively from Amapá and Pernambuco States, Brazil. Hydraena (H.) josefinae sp. nov. is a member of the scintillabella subgroup of the leechi group, while H. (H.) pernambucana sp. nov. belongs to the orcula complex in the marginicollis subgroup of the marginicollis group. Descriptions and diagnostic characters for both new species are offered; habitus photographs and line drawings of male genitalia of the two new species, as well as drawings of female genitalia of H. josefinae sp. n., are provided. Male genitalia of H. alterra Perkins, 1980 is illustrated and compared with that of the closely related H. josefinae sp. nov. Habitat and distributional data of the two new species are also included.


Assuntos
Besouros , Gastrópodes , Distribuição Animal , Animais , Brasil , Ecossistema , Feminino , Masculino
6.
EuroIntervention ; 16(2): e133-e140, 2020 06 12.
Artigo em Inglês | MEDLINE | ID: mdl-32149709

RESUMO

AIMS: The novel sirolimus-eluting ultra-high molecular weight APTITUDE bioreabsorbable vascular scaffold (BRS) displays higher mechanical strength, expansion capabilities and resistance to fracture compared to other BRS technologies. RENASCENT II is a prospective, multicentre first-in-human clinical study evaluating the clinical performance of the APTITUDE BRS in the treatment of single de novo coronary lesions among patients undergoing percutaneous coronary intervention. METHODS AND RESULTS: The APTITUDE BRS was tested in a prospective study in two countries (Italy and Colombia). Study objectives were angiographic in-scaffold late lumen loss (IS-LLL) measured by quantitative coronary angiography (QCA) and target vessel failure (TVF) defined as the composite rate of cardiac death, target vessel myocardial infarction (TV-MI) or ischaemia-driven target lesion revascularisation (TLR) at 9 and 24 months. A total of 60 patients were enrolled. All patients underwent lesion predilatation and 46 patients (76.7%) underwent post-dilatation. Clinical device and procedural success were 98.3% (59/60 patients) and 100%, respectively. Angiographic late lumen loss was 0.19±0.26 mm at 9 months and 0.3±0.41 mm at 24 months. At 9 months, TVF occurred in 2/59 patients (3.4%) due to TV-MI but there was no TLR. No further cases of TVF, MACE or stent thrombosis were reported up to 24-month follow-up. CONCLUSIONS: In this multicentre prospective study, the APTITUDE BRS was shown to be safe and effective in the treatment of single coronary lesions at 24-month clinical follow-up.


Assuntos
Implantes Absorvíveis , Fármacos Cardiovasculares/administração & dosagem , Doença da Artéria Coronariana/terapia , Reestenose Coronária/tratamento farmacológico , Estenose Coronária/terapia , Stents Farmacológicos , Intervenção Coronária Percutânea/instrumentação , Sirolimo/administração & dosagem , Fármacos Cardiovasculares/uso terapêutico , Angiografia Coronária , Doença da Artéria Coronariana/diagnóstico , Reestenose Coronária/etiologia , Estenose Coronária/diagnóstico , Humanos , Intervenção Coronária Percutânea/efeitos adversos , Estudos Prospectivos , Desenho de Prótese , Sirolimo/uso terapêutico , Tomografia de Coerência Óptica , Resultado do Tratamento
7.
Data Brief ; 26: 104521, 2019 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-31667284

RESUMO

The data presented in this paper is supporting the research article "Estimating wind dispersal potential in Ailanthus altissima: The need to consider the three-dimensional structure of samaras" [1]. We analyzed the estimation of samara's wind dispersal potential through a group of morphological variables that succeed in describing the three-dimensional nature of samaras. We present here a dataset containing 8 morphological variables of 200 samaras belonging to 5 different individuals of the invasive tree Ailanthus altissima (Mill.) Swingle. Additionally, we present the average descent velocity of each of the samaras, which was recorded by releasing 5 times each samara under controlled and reproducible conditions. The data set is structured in a single spreadsheet where we also included the samara and the individual identity code of the tree.

8.
Front Plant Sci ; 9: 727, 2018.
Artigo em Inglês | MEDLINE | ID: mdl-29904391

RESUMO

Crown architecture usually is heterogeneous as a result of foraging in spatially and temporally heterogeneous light environments. Ecologists are only beginning to identify the importance of temporal heterogeneity for light acquisition in plants, especially at the diurnal scale. Crown architectural heterogeneity often leads to a diurnal variation in light interception. However, maximizing light interception during midday may not be an optimal strategy in environments with excess light. Instead, long-lived plants are expected to show crown architectures and leaf positions that meet the contrasting needs of light interception and avoidance of excess light on a diurnal basis. We expected a midday depression in the diurnal course of light interception both at the whole-crown and leaf scales, as a strategy to avoid the interception of excessive irradiance. We tested this hypothesis in a population of guava trees (Psidium guajava L.) growing in an open tropical grassland. We quantified three crown architectural traits: intra-individual heterogeneity in foliage clumping, crown openness, and leaf position angles. We estimated the diurnal course of light interception at the crown scale using hemispheric photographs, and at the leaf scale using the cosine of solar incidence. Crowns showed a midday depression in light interception, while leaves showed a midday peak. These contrasting patterns were related to architectural traits. At the crown scale, the midday depression of light interception was linked to a greater crown openness and foliage clumping in crown tops than in the lateral parts of the crown. At the leaf scale, an average inclination angle of 45° led to the midday peak in light interception, but with a huge among-leaf variation in position angles. The mismatch in diurnal course of light interception at crown and leaf scales can indicate that different processes are being optimized at each scale. These findings suggest that the diurnal course of light interception may be an important dimension of the resource acquisition strategies of long-lived woody plants. Using a temporal approach as the one applied here may improve our understanding of the diversity of crown architectures found across and within environments.

9.
Catheter Cardiovasc Interv ; 92(3): E246-E253, 2018 09 01.
Artigo em Inglês | MEDLINE | ID: mdl-29243353

RESUMO

OBJECTIVES: In this first-in-human study, we prospectively studied the vascular compatibility and mechanical performance of a novel bare ultra-high molecular weight amorphous PLLA bioresorbable scaffold (BRS, FORTITUDE®, Amaranth Medical, Mountain View, California) up to two years after implantation using multimodality imaging techniques. BACKGROUND: The vascular biocompatibility of polymers used in BRS has not been fully characterized in the absence of anti-proliferative drugs. METHODS: A total of 10 patients undergoing single scaffold implantation were included in the final analysis and were followed up using optical coherence tomography (OCT) at 2-years. All devices were implanted under angiographic and intravascular ultrasound (IVUS) guidance. Angiographic and IVUS follow up was performed at 6 months. Additionally, angiography and OCT imaging were performed at 2-years. RESULTS: At 6 months, mean intra-scaffold angiographic MLD slightly decreased from baseline procedural values. However, at 2 years, mean angiographic MLD increased (post procedure; 2.9 [2.7, 3.1] mm vs. 6 months; 2.1 [1.6, 2.5] vs. 2 years; 2.4 [2.1, 2.6], P = .001). Also, angiographic percent diameter stenosis decreased and late lumen gain increased between 6 months and 2 years follow up. Mean neointimal hyperplasia volume assessed by IVUS at 6 months was 26% [15.2, 29.3]. At 2 years OCT follow up neointimal hyperplasia volume was 24.2% [19.4, 28.9]. No presence of neoatherosclerosis was identified in any of the analyzed cases. CONCLUSION: At 2 years, this novel PLLA-based BRS induced expansive vascular remodeling from 6 to 24 months, a biological phenomenon that appears to be independent of the presence of anti-proliferative drugs.


Assuntos
Implantes Absorvíveis , Angiografia Coronária , Doença da Artéria Coronariana/terapia , Vasos Coronários/diagnóstico por imagem , Intervenção Coronária Percutânea/instrumentação , Poliésteres/química , Tomografia de Coerência Óptica , Ultrassonografia de Intervenção , Remodelação Vascular , Idoso , Doença da Artéria Coronariana/diagnóstico por imagem , Doença da Artéria Coronariana/patologia , Doença da Artéria Coronariana/fisiopatologia , Vasos Coronários/patologia , Vasos Coronários/fisiopatologia , Estudos de Viabilidade , Feminino , Humanos , Hiperplasia , Masculino , Pessoa de Meia-Idade , Peso Molecular , Imagem Multimodal , Neointima , Intervenção Coronária Percutânea/efeitos adversos , Valor Preditivo dos Testes , Estudos Prospectivos , Desenho de Prótese , Fatores de Tempo , Resultado do Tratamento
10.
Open Heart ; 4(1): e000521, 2017.
Artigo em Inglês | MEDLINE | ID: mdl-28674616

RESUMO

OBJECTIVE: To evaluate the biological effect of microengineered stent grooves (MSG) on early strut healing in humans by performing optical coherence tomography (OCT) analysis 3 weeks following the implantation. BACKGROUND: In the experimental setting, MSG accelerate endothelial cell migration and reduce neointimal proliferation compared with bare metal stent (BMS). METHODS: A total of 37 patients undergoing percutaneous coronary intervention with de novo coronary lesions were randomly assigned to either MSG (n=19) or an identical BMS controls (n=18). All patients underwent OCT imaging at 3 weeks. A total of 7959 struts were included in the final analysis. RESULTS: At 3 weeks following stent implantation, almost all struts analysed (~97%) had evidence of tissue coverage. The percentage of partially covered struts was comparable between both groups. However, the percentage of fully embedded struts was higher in the BMS group (81.22%, 49.75-95.52) compared with the MSG group (74.21%, 58.85-86.38). The stent-level analysis demonstrated reduction in neointimal formation (neointimal hyperplasia area and volume reduction of ~14% and ~19%, respectively) in the MSG versus the BMS group. In the strut-level analysis, an even greater reduction (~22% in neointimal thickness) was seen in the MSG group. Layered neointimal was present in ~6% of the OCT frames in the BMS group while it was not present in the MSG group. CONCLUSIONS: MSG induced a more homogeneous and predictable pattern of surface healing in the early stages following stent implantation. The biological effect of MSG on stent healing has the potential to improve the safety profile of current generation drug-eluting stents. CLASSIFICATIONS: BMS, OCT, clinical trials.

11.
Zootaxa ; 4238(4): 451-498, 2017 Mar 06.
Artigo em Inglês | MEDLINE | ID: mdl-28603247

RESUMO

Preimaginal stages of the six species of Hydraenidae presently known from Cuba were obtained by rearing adults in the laboratory. Eggs of Hydraena perkinsi Spangler, 1980, H. decui Spangler, 1980 and H. franklyni Deler-Hernández & Delgado, 2012 are described and illustrated for the first time. The first instar larva of Gymnochthebius fossatus (LeConte, 1855) is redescribed, adding some new remarkable morphological characters including what could be the first abdominal egg-burster reported for this family. All larval instars of H. perkinsi, H. guadelupensis Orchymont, 1923 and Ochthebius attritus LeConte, 1878 are described and illustrated for the first time, with a special emphasis on their chaetotaxy. The second instar larva of G. fossatus along with first and third instar larvae of H. decui and H. franklyni are also studied for the first time. The pupal morphology and vestiture of a species belonging to the genus Hydraena are described for the first time, based on the pupa of H. perkinsi. Biological notes for several preimaginal stages of the studied species are also given.


Assuntos
Besouros , Animais , Cuba , Larva , Óvulo , Pupa
12.
Zootaxa ; 4250(5): 434-446, 2017 Apr 07.
Artigo em Inglês | MEDLINE | ID: mdl-28610000

RESUMO

Two new species of the genus Hydraena Kugelann, 1794 collected from hygropetric habitats in eastern Cuba are described: Hydraena (Hydraenopsis) blancae sp. nov. from the Nipe-Sagua-Baracoa mountain range, and Hydraena (Hydraenopsis) matthiasi sp. nov. from the Sierra Maestra mountain range. Both species, especially the latter, are closely related to Hydraena (Hydraenopsis) franklyni Deler-Hernández & Delgado, 2012. Diagnostic characters for both new species are provided and illustrated; habitat information and distributional data are also included. An updated key to Cuban species of Hydraena is provided. With this study, the number of species of Hydraena known from Cuba raises to six.


Assuntos
Besouros , Animais , Cuba , Ecossistema
13.
Coron Artery Dis ; 28(2): 126-134, 2017 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-27879518

RESUMO

AIMS: Bioresorbable polymer drug-eluting stent technologies have been considered to have the potential to enhance vascular healing by reducing polymer exposure to the vessel wall, potentially allowing the earlier discontinuation of dual antiplatelet therapy. At present, the early vascular healing response to this type of technologies is still unclear. PATIENTS AND METHODS: The TIMELESS study is a multicenter, prospective, single-arm study that enrolled real-world patients undergoing percutaneous coronary intervention. All patients underwent Synergy stent implantation, which consists of a platinum-chromium platform coated with an ultra-thin abluminal bioabsorbable poly-D,L-lactide-co-glycolide polymer-eluting everolimus. RESULTS: A total of 37 patients were included in the study. The majority of the patients underwent percutaneous coronary intervention because of acute coronary syndromes. At 3 months, angiographic follow-up showed a percentage diameter of stenosis of 8.1±7.5% and an angiographic late loss of 0.03±0.24 mm. In all analyzed struts, less than 1% of struts were definitely uncovered or covered with fibrin, 12.5% (5.0-18.5%) showed evidence of partial coverage, and the remaining (∼85%) were fully covered. No stent thrombosis was observed up to 12 months of clinical follow-up. CONCLUSION: In a real-world population, the implantation of a bioresorbable polymer drug-eluting stent resulted in almost complete strut coverage throughout the entire stent length at 3 months. The clinical implications for antiplatelet therapy and outcomes should be investigated further.


Assuntos
Implantes Absorvíveis , Síndrome Coronariana Aguda/terapia , Fármacos Cardiovasculares/administração & dosagem , Doença da Artéria Coronariana/terapia , Estenose Coronária/terapia , Vasos Coronários/efeitos dos fármacos , Stents Farmacológicos , Everolimo/administração & dosagem , Ácido Láctico/química , Intervenção Coronária Percutânea/instrumentação , Ácido Poliglicólico/química , Tomografia de Coerência Óptica , Cicatrização/efeitos dos fármacos , Síndrome Coronariana Aguda/diagnóstico por imagem , Idoso , Fármacos Cardiovasculares/efeitos adversos , Cromo , Angiografia Coronária , Doença da Artéria Coronariana/diagnóstico por imagem , Estenose Coronária/diagnóstico por imagem , Vasos Coronários/diagnóstico por imagem , Quimioterapia Combinada , Everolimo/efeitos adversos , Feminino , Humanos , Masculino , Intervenção Coronária Percutânea/efeitos adversos , Inibidores da Agregação Plaquetária/administração & dosagem , Platina , Copolímero de Ácido Poliláctico e Ácido Poliglicólico , Valor Preditivo dos Testes , Desenho de Prótese , Fatores de Tempo , Resultado do Tratamento
14.
Zookeys ; (623): 75-88, 2016.
Artigo em Inglês | MEDLINE | ID: mdl-27843389

RESUMO

Hydraena matyotisp. n. (Coleoptera, Hydraenidae) is described from the Seychelles, Indian Ocean. Hydraena mahensis Scott, 1913 is redescribed. The latter is here recorded from La Digue for the first time. A key to the species of the genus Hydraena Kugelann, 1794 of the Seychelles is presented.

15.
Ann Bot ; 117(4): 643-51, 2016 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-26944783

RESUMO

BACKGROUND: The spatial arrangement and expression of foliar syndromes within tree crowns can reflect the coupling between crown form and function in a given environment. Isolated trees subjected to high irradiance and concomitant stress may adjust leaf phenotypes to cope with environmental gradients that are heterogeneous in space and time within the tree crown. The distinct expression of leaf phenotypes among crown positions could lead to complementary patterns in light interception at the crown scale. METHODS: We quantified eight light-related leaf traits across 12 crown positions of ten isolated Olea europaea trees in the field. Specifically, we investigated whether the phenotypic expression of foliar traits differed among crown sectors and layers and five periods of the day from sunrise to sunset. We investigated the consequences in terms of the exposed area of the leaves at the tree scale during a single day. KEY RESULTS: All traits differed among crown positions except the length-to-width ratio of the leaves. We found a strong complementarity in the patterns of the potential exposed area of the leaves among day periods as a result of a non-random distribution of leaf angles across the crown. Leaf exposure at the outer layer was below 60 % of the displayed surface, reaching maximum interception during morning periods. Daily interception increased towards the inner layer, achieving consecutive maximization from east to west positions within the crown, matching the sun's trajectory. CONCLUSIONS: The expression of leaf traits within isolated trees of O. europaea varies continuously through the crown in a gradient of leaf morphotypes and leaf angles depending on the exposure and location of individual leaves. The distribution of light-related traits within the crown and the complementarity in the potential exposure patterns of the leaves during the day challenges the assumption of low trait variability within individuals.


Assuntos
Luz , Olea/fisiologia , Olea/efeitos da radiação , Folhas de Planta/fisiologia , Folhas de Planta/efeitos da radiação , Árvores/fisiologia , Árvores/efeitos da radiação , Modelos Lineares , Característica Quantitativa Herdável , Fatores de Tempo
16.
PLoS One ; 9(10): e107709, 2014.
Artigo em Inglês | MEDLINE | ID: mdl-25272037

RESUMO

Insect phylogeny has recently been the focus of renewed interest as advances in sequencing techniques make it possible to rapidly generate large amounts of genomic or transcriptomic data for a species of interest. However, large numbers of markers are not sufficient to guarantee accurate phylogenetic reconstruction, and the choice of the model of sequence evolution as well as adequate taxonomic sampling are as important for phylogenomic studies as they are for single-gene phylogenies. Recently, the sequence of the genome of a strepsipteran has been published and used to place Strepsiptera as sister group to Coleoptera. However, this conclusion relied on a data set that did not include representatives of Neuropterida or of coleopteran lineages formerly proposed to be related to Strepsiptera. Furthermore, it did not use models that are robust against the long branch attraction artifact. Here we have sequenced the transcriptomes of seven key species to complete a data set comprising 36 species to study the higher level phylogeny of insects, with a particular focus on Neuropteroidea (Coleoptera, Strepsiptera, Neuropterida), especially on coleopteran taxa considered as potential close relatives of Strepsiptera. Using models robust against the long branch attraction artifact we find a highly resolved phylogeny that confirms the position of Strepsiptera as a sister group to Coleoptera, rather than as an internal clade of Coleoptera, and sheds new light onto the phylogeny of Neuropteroidea.


Assuntos
Genômica , Insetos/classificação , Insetos/genética , Filogenia , Animais , Feminino , Masculino , Modelos Genéticos , RNA Ribossômico
17.
PLoS One ; 9(4): e93614, 2014.
Artigo em Inglês | MEDLINE | ID: mdl-24709670

RESUMO

Derived members of the endoparasitic order Strepsiptera have acquired an extreme form of sexual dimorphism whereby males undergo metamorphosis and exist as free-living adults while females remain larviform, reaching sexual maturity within their hosts. Expression of the transcription factor, broad (br) has been shown to be required for pupal development in insects in which both sexes progress through metamorphosis. A surge of br expression appears in the last larval instar, as the epidermis begins pupal development. Here we ask if br is also up-regulated in the last larval instar of male Xenos vesparum Rossi (Stylopidae), and whether such expression is lost in neotenic larviform females. We clone three isoforms of br from X. vesparum (Xv'br), and show that they share greatest similarity to the Z1, Z3 and Z4 isoforms of other insect species. By monitoring Xv'br expression throughout development, we detect elevated levels of total br expression and the Xv'Z1, Xv'Z3, and Xv'Z4 isoforms in the last larval instar of males, but not females. By focusing on Xv'br expression in individual samples, we show that the levels of Xv'BTB and Xv'Z3 in the last larval instar of males are bimodal, with some males expressing 3X greater levels of Xv'br than fourth instar femlaes. Taken together, these data suggest that neoteny (and endoparasitism) in females of Strepsiptera Stylopidia could be linked to the suppression of pupal determination. Our work identifies a difference in metamorphic gene expression that is associated with neoteny, and thus provides insights into the relationship between metamorphic and neotenic development.


Assuntos
Regulação da Expressão Gênica/fisiologia , Proteínas de Insetos/biossíntese , Insetos/metabolismo , Metamorfose Biológica/fisiologia , Fatores de Transcrição/biossíntese , Animais , Feminino , Proteínas de Insetos/genética , Insetos/genética , Masculino , Pupa/genética , Pupa/metabolismo , Fatores de Transcrição/genética
18.
J Am Coll Cardiol ; 61(15): 1596-600, 2013 Apr 16.
Artigo em Inglês | MEDLINE | ID: mdl-23500318

RESUMO

OBJECTIVES: The aim of this study was to evaluate the safety as well as the clinical and technical effectiveness of robotic-assisted percutaneous coronary intervention. BACKGROUND: Robotic systems have been suggested to enhance the performance of cardiovascular procedures, as well as to provide protection from the occupational hazards that are associated with interventional practice. METHODS: Patients with coronary artery disease and clinical indications for percutaneous intervention were enrolled. The coronary intervention was performed with the CorPath 200 robotic system, which consists of a remote interventional cockpit and a bedside disposable cassette that enables the operator to advance, retract, and rotate guidewires and catheters. The primary endpoints were clinical procedural success, defined as <30% residual stenosis at the completion of the robotic-assisted procedure without major adverse cardiovascular events within 30 days, and device technical success, defined as the successful manipulation of the intracoronary devices using the robotic system only. RESULTS: A total of 164 patients were enrolled at 9 sites. Percutaneous coronary intervention was completed successfully without conversion to manual operation, and device technical success was achieved in 162 of 164 patients (98.8%). There were no device-related complications. Clinical procedural success was achieved in 160 of 164 patients (97.6%), whereas 4 (2.4%) had periprocedural non-Q-wave myocardial infarctions. No deaths, strokes, Q-wave myocardial infarctions, or revascularization occurred in the 30 days after the procedures. Radiation exposure for the primary operator was 95.2% lower than the levels found at the traditional table position. CONCLUSIONS: This pivotal multicenter study with a robotic-enhanced coronary intervention system demonstrated the safety and feasibility of the system. The robotic remote-control procedure met the expected technical and clinical performance, with significantly lower radiation exposure to the operator. (Evaluation of the Safety and Effectiveness of the CorPath 200 System in Percutaneous Coronary Interventions [PCI] [PRECISE]; NCT01275092).


Assuntos
Doença da Artéria Coronariana/cirurgia , Infarto do Miocárdio , Exposição Ocupacional/prevenção & controle , Intervenção Coronária Percutânea , Complicações Pós-Operatórias/epidemiologia , Robótica , Idoso , Catéteres , Angiografia Coronária , Doença da Artéria Coronariana/diagnóstico , Vasos Coronários/patologia , Vasos Coronários/cirurgia , Desenho de Equipamento/métodos , Desenho de Equipamento/normas , Estudos de Viabilidade , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Infarto do Miocárdio/epidemiologia , Infarto do Miocárdio/etiologia , Salas Cirúrgicas , Avaliação de Processos e Resultados em Cuidados de Saúde , Intervenção Coronária Percutânea/efeitos adversos , Intervenção Coronária Percutânea/métodos , Intervenção Coronária Percutânea/estatística & dados numéricos , Saúde Radiológica , Medição de Risco , Robótica/métodos , Robótica/estatística & dados numéricos , Stents , Resultado do Tratamento , Recursos Humanos
19.
Insects ; 4(3): 463-75, 2013 Sep 04.
Artigo em Inglês | MEDLINE | ID: mdl-26462430

RESUMO

The unusual life style of Strepsiptera has presented a long-standing puzzle in establishing its affinity to other insects. Although Strepsiptera share few structural similarities with other insect orders, all members of this order share a parasitic life style with members of two distinctive families in the Coleoptera-the order now considered the most closely related to Strepsiptera based on recent genomic evidence. Among the structural features of several strepsipteran families and other insect families that have been surveyed are the organization of testes and ultrastructure of sperm cells. For comparison with existing information on insect sperm structure, this manuscript presents a description of testes and sperm of a representative of the most primitive extant strepsipteran family Mengenillidae, Eoxenos laboulbenei. We compare sperm structure of E. laboulbenei from this family with that of the three other families of Strepsiptera in the other strepsipteran suborder Stylopidia that have been studied as well as with members of the beetle families Meloidae and Rhipiphoridae that share similar life histories with Strepsiptera. Meloids, Rhipiphorids and Strepsipterans all begin larval life as active and viviparous first instar larvae. This study examines global features of these insects' sperm cells along with specific ultrastructural features of their organelles.

20.
Zootaxa ; 3700: 195-9, 2013.
Artigo em Inglês | MEDLINE | ID: mdl-26106722

RESUMO

Ochthebius hivae is described from western Iran (Ilam and Khuzestan Provinces). It belongs to the O. metallescens Rosenhauer, 1847 species group. The aedeagal distal lobe of this species is remarkably variable.


Assuntos
Besouros/anatomia & histologia , Besouros/classificação , Distribuição Animal , Animais , Feminino , Irã (Geográfico) , Masculino , Caracteres Sexuais , Especificidade da Espécie
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