RESUMO
Comparability of information gathered by different methods is vital to enhance knowledge in heterogeneous catalysis. A new type of flow-reactor has been developed which enables the comparison between the detailed information gained by surface science methods and industrial catalysis, thus contributing to bridge the pressure and material gaps. The design allows for catalytic investigations of compact, low-surface area materials at temperatures and pressures up to 500 °C and 10 bar, respectively. Catalytic measurements on pressed pills of Pd11Bi2Se2 in the semi-hydrogenation of acetylene and oriented single-crystalline slabs of InPd in methanol steam reforming are used as test cases for the reactor design. In the former, high-conversion of acetylene is demonstrated along with ensured inert sample transfer. In the latter, higher catalytic activity for the (110) surface is observed compared to the (100) and (111) surfaces. Most importantly, both test cases prove the viability of the reactor design, which opens new possibilities for studying different materials and systems.
RESUMO
The intermetallic compound ZnPd has demonstrated excellent catalytic properties in methanol steam reforming. While it is known that defects and microstructures influence the catalytic properties, little is known about the defects occurring in ZnPd. Due to recent advances in synthetic methods, coarse-grained ZnPd samples are accessible. This enables the detection and investigation of twinning in ZnPd by studying the twinned regions from the macroscopic scale by polarised light and electron backscattering diffraction (EBSD) down to the atomic scale by high-resolution transmission electron microscopy (HR-TEM). Twinning occurs in {101} and is coupled with a change in the c/a ratio in the vicinity of the twin boundary. Quantum chemical calculations result in only very small energy differences between the ideal and the twinned structure, explaining the experimentally observed thermal stability of the latter. The chemical bonding was investigated by the electron localizability indicator (ELI) and compared to the one in the ideal structure. The results confirm twinning along the {101} plane and demonstrate the high stability of the twin boundaries after formation.
RESUMO
Two cases of successful molecular replacement using NMR trial models are presented. One is the crystal structure of the Escherichia coli colicin immunity protein Im7; the other is a heretofore unreported crystal structure of a specific PDGF receptor-derived peptide complex of the carboxy-terminal SH2 domain from the p85alpha subunit of human phosphatidylinositol 3-OH kinase. In both cases, molecular replacement was non-trivial. Success was achieved using trial models that consisted of an ensemble of NMR structures from which the more flexible portions had been excised. Use of maximum-likelihood refinement proved critical to be able to refine the poor starting models. The challenges typical of the use of NMR trial models in molecular replacement are discussed.
Assuntos
Proteínas de Bactérias/química , Colicinas , Cristalografia por Raios X , Espectroscopia de Ressonância Magnética , Modelos Moleculares , Fosfatidilinositol 3-Quinases/química , Humanos , Fragmentos de Peptídeos/química , Fragmentos de Peptídeos/metabolismo , Fosfatidilinositol 3-Quinases/metabolismo , Conformação Proteica , Receptores do Fator de Crescimento Derivado de Plaquetas/química , Domínios de Homologia de srcRESUMO
We report the first detailed comparison of two immunity proteins which, in conjunction with recent protein engineering data, begins to explain how these structurally similar proteins are able to bind and inhibit the endonuclease domain of colicin E9 (E9 DNase) with affinities that differ by 12 orders of magnitude. In the present work, we have determined the X-ray structure of the Escherichia coli colicin E7 immunity protein Im7 to 2.0 A resolution by molecular replacement, using as a trial model the recently determined NMR solution structure of Im9. Whereas the two proteins adopt similar four-helix structures, subtle structural differences, in particular involving a conserved tyrosine residue critical for E9 DNase binding, and the identity of key residues in the specificity helix, lie at the heart of their markedly different ability to bind the E9 DNase. Two other crystal structures were reported recently for Im7; in one, Im7 was a monomer and was very similar to the structure reported here, whereas in the other it was a dimer to which functional significance was assigned. Since this previous work suggested that Im7 could exist either as a monomer or a dimer, we used analytical ultracentrifugation to investigate this question further. Under a variety of solution conditions, we found that Im7 only ever exists in solution as a monomer, even up to protein concentrations of 15 mg/ml, casting doubt on the functional significance of the crystallographically observed dimer. This work provides a structural framework with which we can understand immunity-protein specificity, and in addition we believe it to be the first successfully refined crystal structure solved by molecular replacement using an NMR trial model with less than 100% sequence identity.
Assuntos
Proteínas de Bactérias/química , Proteínas de Escherichia coli , Escherichia coli/química , Conformação Proteica , Sequência de Aminoácidos , Proteínas de Bactérias/metabolismo , Biopolímeros , Colicinas/metabolismo , Cristalografia por Raios X , Eletricidade , Endonucleases/metabolismo , Escherichia coli/metabolismo , Modelos Moleculares , Dados de Sequência Molecular , Ressonância Magnética Nuclear Biomolecular , Tirosina/química , Tirosina/metabolismo , UltracentrifugaçãoRESUMO
The colicin DNase-specific immunity proteins interact with the endonuclease domain of the bacterial toxin colicin E9 with dissociation constants that span the millimolar to femtomolar affinity range. Among the non-cognate interactions Im2 shows the strongest binding toward the E9 DNase domain with a Kd of 10(-8) M, 6 orders of magnitude weaker than that of the cognate immunity protein Im9. Based on a NMR structure of Im9 that shows it to be a 4-helix protein, we have conducted a mutagenic scan in which elements of Im9 secondary structure were substituted into Im2 to precisely delineate regions that define specificity. Eleven chimeras were constructed, and their biological cross-reactivity toward colicins E2 and E9 was evaluated. From this set of mutants seven proteins were purified, and the Kd for their interaction with the E9 DNase domain was measured by a combination of stopped-flow fluorescence and subunit exchange kinetics. Our results show that immunity specificity is dominated by residues on helix II, accounting for 5 orders of magnitude binding specificity relative to Im2, and that packing interactions of helix II with its neighbor helix I and the loop connecting helix III with helix IV play minor roles. The conformational stability of these chimeric proteins was also determined. Proteins displaying an Im9 phenotype were all more stable than the parent Im2 protein, and surprisingly some chimeras were significantly more stable than either Im2 or Im9.
Assuntos
Proteínas de Bactérias/metabolismo , Colicinas , Proteínas de Escherichia coli , Sequência de Aminoácidos , Desoxirribonucleases/metabolismo , Escherichia coli , Guanidina , Guanidinas , Cinética , Modelos Moleculares , Dados de Sequência Molecular , Mutagênese Sítio-Dirigida , Ligação Proteica , Desnaturação Proteica , Dobramento de Proteína , Estrutura Secundária de Proteína , Proteínas Recombinantes de Fusão/metabolismo , Alinhamento de SequênciaRESUMO
OBJECTIVES: This study compared exercise and pharmacologic stress testing using arbutamine delivered by a closed-loop device for the detection of coronary artery disease. BACKGROUND: Arbutamine, an agent designed to simulate exercise, has been developed in conjunction with a closed-loop delivery device that modulates the rate of administration on the basis of physiologic feedback. METHODS: Two hundred ten patients (180 men, 30 women) with symptoms and angiographic evidence of coronary artery disease were studied. Ischemia was categorized in three ways: 1) the presence of angina; 2) the occurrence of > or = 0.1-mV horizontal or downsloping ST segment depression or elevation at 60 ms after the J point; or 3) the presence of either condition 1 or 2. RESULTS: In the 210 patients, the mean increase in heart rate and systolic blood pressure evoked by arbutamine and exercise was 51 and 53 beats/min (p = NS) and 36 and 44 mm Hg (p < 0.0001), respectively. Arbutamine detected ischemia more often than exercise with each of the three ischemic end points. Sensitivity for detecting ischemia by either angina or ST segment change was 84% (95% confidence interval ¿ change was 84% (95% confidence interval [CI] 79% to 89%) for arbutamine and 75% (95% CI 69% to 81%) for exercise testing (p = 0.014). For angina alone, sensitivity was 73% (95% CI 67% to 79%) for arbutamine and 64% (95% CI 57% to 71%) for exercise (p = 0.026). For ST segment change alone, sensitivity was 47% (95% CI 40% to 54%) for arbutamine and 44% (95% CI 37% to 51%) for exercise (p = 0.426). Cardiac events occurred in five patients (1.8%) within 24 h of the arbutamine test. CONCLUSIONS: In detecting documented coronary artery disease, the sensitivity of arbutamine testing was equal to that of exercise for the electrocardiographic end point of ST segment change alone. Arbutamine testing was significantly superior to exercise testing for either ST change or angina or for angina alone.
Assuntos
Cardiotônicos , Catecolaminas , Doença das Coronárias/diagnóstico , Idoso , Cardiotônicos/administração & dosagem , Catecolaminas/administração & dosagem , Doença das Coronárias/fisiopatologia , Sistemas de Liberação de Medicamentos , Teste de Esforço , Feminino , Hemodinâmica , Humanos , Masculino , Pessoa de Meia-Idade , Sensibilidade e EspecificidadeRESUMO
Respiratory failure is one of the most uncommon and serious adverse drug reactions. Low-molecular-weight-dextran (Dextran-40) is a useful adjunctive anti-platelet agent in the setting of coronary angioplasty and intracoronary stent placement. We report the occurrence of the adult respiratory distress syndrome following intravenous infusion of Dextran-40.
Assuntos
Dextranos/efeitos adversos , Edema Pulmonar/induzido quimicamente , Síndrome do Desconforto Respiratório/induzido quimicamente , Adulto , Angioplastia Coronária com Balão/métodos , Doença da Artéria Coronariana/terapia , Dextranos/administração & dosagem , Feminino , Humanos , Infusões IntravenosasRESUMO
OBJECTIVE: To evaluate the efficacy of a physician-directed, nurse-managed, home-based case-management system for coronary risk factor modification. DESIGN: Randomized clinical trial in which patients received a special intervention (n = 293) or usual medical care (n = 292) during the first year after acute myocardial infarction. SETTING: 5 Kaiser Permanente Medical Centers in the San Francisco Bay area. PATIENTS: 585 men and women aged 70 years or younger who were hospitalized for acute myocardial infarction. INTERVENTION: In the hospital, specially trained nurses initiated interventions for smoking cessation, exercise training, and diet-drug therapy for hyperlipidemia. Intervention after discharge was implemented primarily by telephone and mail contact with patients in their homes. All medically eligible patients received exercise training; all smokers received the smoking cessation intervention; and all patients received dietary counseling and, if needed, lipid-lowering drug therapy. OUTCOME: Smoking prevalence and plasma low-density lipoprotein cholesterol (LDL) concentrations were measured 2 months after infarction, and functional capacity was measured 6 months after infarction. RESULTS: In the special intervention and usual care groups, the cotinine-confirmed smoking cessation rates were 70% and 53% (P = 0.03), plasma LDL cholesterol levels were 2.77 +/- 0.69 mmol/L and 3.41 +/- 0.90 mmol/L (107 +/- 30 mg/dL and 132 +/- 30 mg/dL) (P = 0.001), and functional capacities were 9.3 +/- 2.4 METS and 8.4 +/- 2.5 METS (P = 0.001), respectively. CONCLUSION: In a large health maintenance organization, a case-management system was considerably more effective than usual medical care for modification of coronary risk factors after myocardial infarction.
Assuntos
Terapia por Exercício , Hipercolesterolemia/tratamento farmacológico , Programas de Assistência Gerenciada , Infarto do Miocárdio/reabilitação , Abandono do Hábito de Fumar , Adulto , Idoso , LDL-Colesterol/sangue , Fatores de Confusão Epidemiológicos , Aconselhamento , Feminino , Humanos , Hipercolesterolemia/complicações , Hipercolesterolemia/dietoterapia , Masculino , Pessoa de Meia-Idade , Infarto do Miocárdio/sangue , Infarto do Miocárdio/etiologia , Fenômenos Fisiológicos da Nutrição , Equipe de Assistência ao Paciente , Fatores de Risco , São Francisco , Fumar/efeitos adversos , Resultado do TratamentoRESUMO
Selective infusion of urokinase into occluded coronary bypass vein grafts is effective in restoring patency. We report the occurrence of intracerebral hemorrhage complicating an intra-graft urokinase infusion protocol. The patient had known cerebral vascular structural pathology without recent clinical complications. Caution with the use of thrombolysis in this setting is suggested.
Assuntos
Hemorragia Cerebral/induzido quimicamente , Ponte de Artéria Coronária , Oclusão de Enxerto Vascular/tratamento farmacológico , Terapia Trombolítica/efeitos adversos , Ativador de Plasminogênio Tipo Uroquinase/efeitos adversos , Contraindicações , Humanos , Infusões Intravenosas , Masculino , Pessoa de Meia-Idade , Veia Safena/transplante , Fatores de Tempo , Ativador de Plasminogênio Tipo Uroquinase/administração & dosagem , Ativador de Plasminogênio Tipo Uroquinase/uso terapêuticoRESUMO
The capacity of cardiac patients to work in their occupations reflects a complex interaction of medical and nonmedical factors. Medical considerations include prognosis and the ability of patients to tolerate the physical, environmental and psychological aspects of their occupation. Nonmedical factors include the patient's satisfaction with the job, economic motivation to work and perceived risk of continued work. Patients' perceptions of their capacity to work and the risks of such work are especially important determinants of occupational work status after myocardial infarction and coronary operations. Symptom-limited treadmill exercise testing carried out three to four weeks after the acute event not only clarifies prognosis and quantitates functional capacity but helps patients to realistically assess their capacity for work. Approximately half of postinfarction patients are found by such testing to have a very low first-year mortality of less than 2 percent. Functional capacity is well maintained in these patients: they do not require formal reconditioning in order to resume their occupational work soon (three to five weeks) after infarction. Exercise testing performed soon after myocardial infarction and coronary artery operation affords practical guidelines for clearing a person to return to work and obviates much of the medically unwarranted disability that follows these events.
Assuntos
Doença das Coronárias/reabilitação , Avaliação da Deficiência , Avaliação da Capacidade de Trabalho , Angina Pectoris/diagnóstico , Doença das Coronárias/cirurgia , Humanos , Infarto do Miocárdio/reabilitação , Período Pós-OperatórioAssuntos
Teste de Esforço , Infarto do Miocárdio/fisiopatologia , Adulto , Idoso , Arritmias Cardíacas/diagnóstico , Arritmias Cardíacas/etiologia , Doença das Coronárias/diagnóstico , Doença das Coronárias/etiologia , Eletrocardiografia , Estudos de Avaliação como Assunto , Hospitalização , Humanos , Masculino , Pessoa de Meia-Idade , Infarto do Miocárdio/complicações , Infarto do Miocárdio/terapia , Fatores de TempoRESUMO
Turnover of 125I-low density lipoprotein (LDL) and of 131I-high density lipoprotein (HDL) was determined before and after end-to-side portacaval shunt in eight swine. LDL (d 1.019-1.063) and HDL (d.1.09-1.21) were isolated by ultracentrifugation and iodinated by the iodine monochloride technique. Immediately postoperatively there was no consistent change in the fractional catabolic rate (FCR) of LDL compared to preoperative control values, while in all animals FCR of HDL was significantly increased (by as much as 300%). After recovery from surgery, neither LDL nor HDL catabolic rates were significantly elevated above control values in four swine. However, plasma levels of LDL and HDL protein, and of LDL and HDL cholesterol were significantly reduced 10-12 weeks after the portacaval shunt. The reduced levels of LDL and HDL associated with normal fractional clearance rates imply a reduction in synthesis of LDL and HDL following portal diversion.
Assuntos
Lipoproteínas HDL/sangue , Lipoproteínas LDL/sangue , Animais , Colesterol/sangue , Ésteres do Colesterol/sangue , Iodoproteínas/sangue , Cinética , Derivação Portocava Cirúrgica , SuínosRESUMO
Minicell-producing strains of Escherichia coli K-12 seem to produce extra membranous material yielding internal cross membranes, "piggy-back" minicell forms, and unusual vesicles.