RESUMO
This study presents the application of Y-chromosomal STR polymorphisms to male identification in the case of a serial rapist and woman murderer in Poland. Since August 1996 a rapist from Swinoujscie (northwest Poland) committed at least 14 rapes. In the year 2000 he brutally raped 8 young girls and murdered a 22-year-old girl. DNA profiles obtained from semen stains left at the scenes of crime gave information that one and the same man had committed all the rapes. The Y-chromosome haplotype (9 loci) obtained was used for the elimination process of 421 suspects. One man was found who had an identical DNA profile in all Y-chromosome STR loci analysed and possessed common alleles in 9 out of 10 autosomal loci, strongly suggesting that the real rapist and the typed man were closely related males. Analysis of reference DNA obtained from the man's brother revealed an identical DNA STR profile to that identified at the crime scenes. To the best of our knowledge this is the first case in Poland and probably in Eastern Europe where DNA typing of a large population was used to identify the offender.
Assuntos
Cromossomos Humanos Y/genética , Impressões Digitais de DNA/métodos , Estupro , Sequências de Repetição em Tandem , Adulto , Feminino , Genótipo , Homicídio , Humanos , Masculino , Polônia , Reação em Cadeia da Polimerase , Polimorfismo Genético , Reprodutibilidade dos TestesRESUMO
A total of 508 unrelated males from the North Poland population were analyzed for 9 Y-chromosome STRs (DYS 19, DYS 390, DYS 393, DYS 392, DYS 391, DYS 389I, DYS 389II and DYS 385I/II) using two multiplex reactions and detection of PCR products using capillary electrophoresis. In the analyzed sample 328 different haplotypes were identified, among which 264 were unique. It was found that the model for a Polish population haplotype (DYS 19*17, DYS 390*25, DYS 393*13, DYS 392*11, DYS 391*10, DYS 389I*13, DYS 389II*30, DYS 385I/II*10,14) is almost 15 times more frequent in our population than in a cumulative European one. The haplotype diversity/discrimination index calculated for 9 loci is 0.9943. In the analysed population sample three mutations were detected in the DYS19 (duplication) and DYS385I/II loci (triplications).