Degradation of starch in pasta induced by extrusion below gelatinization temperature.

The structural deformation of starch during pasta extrusion leads to varied effects on pasta quality. We investigated the impact of shearing force on the starch structure of pasta and pasta quality by varying the screw speed (100, 300, 500 and 600 rpm) with a temperature range of 25 to 50 ℃ in increments of 5 ℃, from the feeding zone to the die zone. The higher screw speeds were associated with more specific mechanical energy input (157, 319, 440, and 531 kJ/kg for pasta produced at 100, 300, 500 and 600 rpm, respectively), resulting in a lower pasting viscosity (1084, 813, 522 and 480 mPa·s for pasta produced at 100, 300, 500 and 600 rpm, respectively) in the pasta due to the loss of starch molecular order and crystallinity. Size-exclusion chromatography revealed that pasta produced at 600 rpm screw speed had a lower amylopectin size distribution which indicated molecular breakdown during extrusion. Pasta produced at 600 rpm had higher in vitro starch hydrolysis (both raw and cooked) than the pasta made at 100 rpm. The research provides relationship of how the screw speed can be manipulated to design pasta with varied texture and nutritional functionality.

Plant cell wall composition modulates the gut microbiota and metabolites in in-vitro fermentation.

This research investigated the effect of different types of plant cell wall fibres, including cereal (i.e., barley, sorghum, and rice), legume (i.e., pea, faba bean, and mung bean), and tuber (potato, sweet potato, and yam) cell wall fibres on in vitro faecal fermentation profiles and gut microbiota composition. The cell wall composition, specifically the content of lignin and pectin, was found to have a significant influence on the gut microbiota and fermentation outcomes. Compared with type I cell walls (legume and tuber) which have high pectin content, the type II cell walls (cereal) which are high in lignin but low in pectin had a lower fermentation rates and less short-chain fatty acid production. The redundancy analysis showed samples with similar fibre composition and fermentation profiles clustered together, and the principal coordinate analysis revealed separation among different types of cell walls and closer proximity among the same cell wall types. These findings emphasize the importance of cell wall composition in shaping the microbial community during fermentation and contribute to a better understanding of the relationship between plant cell walls and gut health. This research has practical implications for the development of functional foods and dietary interventions.

Hard-to-cook phenomenon in common legumes: Chemistry, mechanisms and utilisation.

Future dietary protein demand will focus more on plant-based sources than animal-based products. In this scenario, legumes and pulses (lentils, beans, chickpeas, etc.) can play a crucial role as they are one of the richest sources of plant proteins with many health benefits. However, legume consumption is undermined due to the hard-to-cook (HTC) phenomenon, which refers to legumes that have high resistance to softening during cooking. This review provides mechanistic insight into the development of the HTC phenomenon in legumes with a special focus on common beans and their nutrition, health benefits, and hydration behaviour. Furthermore, detailed elucidation of HTC mechanisms, mainly pectin-cation-phytate hypothesis and compositional changes of macronutrients like starch, protein, lipids and micronutrients like minerals, phytochemicals and cell wall polysaccharides during HTC development are critically reviewed based on the current research findings. Finally, strategies to improve the hydration and cooking quality of beans are proposed, and a perspective is provided.

Structural and Thermal Characterization of Protein Isolates from Australian Lupin Varieties as Affected by Processing Conditions.

Proteins from the full and defatted flours of L. angustifolius cv Jurien and L. albus cv Murringo were prepared using alkaline extraction and iso-electric precipitation. Isolates were either freeze dried or spray dried or pasteurized at 75 ± 3 °C/5 min before freeze-drying. Various structural properties were investigated to elucidate the varietal and processing-induced effect on molecular and secondary structure. Irrespective of processing, isolated proteins had a similar molecular size, with α-conglutin (412 kDa) and ß-conglutin (210 kDa) being principal fractions for the albus and angustifolius variety, respectively. Smaller peptide fragments were observed for the pasteurized and spray dried samples, indicating some degree of processing-induced changes. Furthermore, secondary structure characterization by Fourier-transform-infrared and circular dichroism spectroscopy showed ß-sheet and α-helical structure being the dominant structure, respectively. Thermal characterization showed two denaturation peaks corresponding to ß-conglutin (Td = 85-89 °C) and α-conglutin (Td = 102-105 °C) fractions. However, the enthalpy values for α-conglutin denaturation were significantly higher for albus species, which corroborates well with higher amounts of heat stable α-conglutin present. Amino acid profile was similar for all samples with limiting sulphur amino acid. In summary, commercial processing conditions did not have a profound effect on the various structural properties of lupin protein isolates, and properties were mainly determined by varietal differences.

Bioactive Nutrient Retention during Thermal-Assisted Hydration of Lupins.

Lupin, an arid pulse, is gaining popularity as a super food due to its superior nutritional properties. However, it has not been considered for large scale thermal processing, e.g., canning. The present work evaluated the best time/temperature combination to hydrate lupins for canning with minimum losses of bioactive nutrients, pre-biotic fibre, and total solids during hydration. The two lupin species showed a sigmoidal hydration behaviour, which was adequately modelled by the Weibull distribution. The effective diffusivity, Deff, increased from 7.41 × 10-11 to 2.08 × 10-10 m2/s for L. albus and 1.75 × 10-10 to 1.02 × 10-9 m2/s for L. angustifolius with increasing temperature, namely, from 25 °C to 85 °C. The lag phase decreased from 145 min to 56 min in L. albus and 61 min to 28 min in L. angustifolius. However, based on the effective hydration rate, reaching the equilibrium moisture, minimum loss of the solids, and prebiotic fibre and phytochemicals, 200 min hydration at 65 °C can be regarded as the optimum temperature of hydration. The findings are thus relevant for designing the hydration protocol to achieve the maximum equilibrium moisture content and yield with the minimum loss of solids (phytochemicals and prebiotic fibres) for L. albus and L. angustifolius.

Mashing performance as a function of malt particle size in beer production.

Significant innovations have occurred over the past 50 years in the malting and brewing industries, focused on optimization of the beer mashing, boiling and fermentation processes. One of the challenges faced in beer brewing has been in the malting process to obtain the desired malt and wort quality to produce high-quality beer products. The hydrolytic enzymes produced during grain germination are mostly entrapped inside the cellular matrices of the grain. The intra-grain diffusion of enzymes for in-situ hydrolysis, as well as diffusion of enzymes to wort, depends upon the malt size and malt size fractions obtained after milling. This review investigates the relationship between varying barley grain particle size distribution and the efficiency of the malting and mashing processes. Recommended ideal particle size of barley grain before and after milling are proposed based on the review of existing literature. Each brewing batch of grains with a proportion of >80% plump grains (>2.5 mm in size) is suggested to be the optimal size before milling, whereas the optimum grain particle size after milling ranged between 0.25 and 0.5 mm. The current review will summarize the theoretical aspects for malt milling and the particle size characteristics for optimizing the brewing process.

Effect of seed coat microstructure and lipid composition on the hydration behavior and kinetics of two red bean (Phaseolus vulgaris L.) varieties.

Bean hydration is a crucial, yet complex process affected both by inherent bean properties (intrinsic) and properties of the soak medium (extrinsic) whose relationship with hydration kinetics are not clear. This work investigated the effect of temperature (30-80°C) on the hydration behavior and kinetics of two commonly consumed Phaseolus vulgaris L. beans-red kidney and small red beans. Both beans have similar morphology (except size), chemical composition and seed coat surface structure but, surprisingly, their hydration behaviors differed markedly. Small red hydration followed a sigmoidal shape (to 70°C) with a prominent lag phase at 30-40°C, while fast hydrating red kidney beans followed a downward concave shape, suggesting different mechanisms of water uptake. The hydration behaviors for red kidney and small red beans were modeled using Peleg (R2 ≥0.97) and sigmoidal (R2 ≥0.99) models, respectively. Water uptake rate increased, while equilibrium moisture content decreased for both bean varieties with increasing temperature. A simplified model was developed for small red beans which can predict hydration as a function of temperature and time with good accuracy ( M mod = M expt . × 1.06 - 0.56 ${M_{{\rm{mod}}}} = {M_{{\rm{expt}}{\rm{.}}}}\ \times 1.06 - 0.56$ , R2  = 0.99, RMSE = 4.5). Microscopic examination of cross sections of seed coats showed nearly double parenchyma layer thickness in small red bean and seed coat lipid analysis showed significantly higher proportion of saturated fatty acids which, together, may be responsible for slower water uptake observed in small red beans. PRACTICAL APPLICATION: Hydration is the crucial stage in dry bean processing, which precedes other processes like cooking, extraction, fermentation, sprouting, and consumption. Industrial bean processing is still slow and batch process and needs improvement. This work shows the effect of various bean properties and soaking medium temperature on the hydration behavior of two commonly consumed red beans. The work also modeled the hydration kinetics of two Phaseolus vulgaris L. beans which can be adopted by bean processors for process design and optimization.

Intact cells: "Nutritional capsules" in plant foods.

Macronutrients of pulses or cereals are stored in the cotyledon or endosperm cells with protection from intact cell walls. However, pulses and cereals are generally processed into fine particles during food production. For example, after milling, the macronutrients enclosed in the intact cells are released and are easily accessible to digestive enzymes in the gastrointestinal tract, leading to high metabolic responses. Therefore, studies on the health effects of intact cells and developing an alternative ingredient with a higher proportion of intact cells are areas of emerging interest. In this review, we highlighted the smallest unit of whole grain, an individual cell, as "nutritional capsules" and elucidated the structure-function of the nutritional capsules, followed by isolation techniques, as a potential novel functional ingredient and food. The polysaccharides' monomeric composition, secondary structure, and interactions determine the cell wall properties including the cell detachment during isolation and isolated cell properties. The intact cellular structure is retained after mild food processing and digestion, thereby, contributing to a lower extent/rate of digestion of entrapped macronutrients. Furthermore, the excursed intact capsules in the colonic environment modulate the population and diversity of microbiota, favouring the increased production of the short-chain fatty acids (SCFAs). The structural schematic model of Type-I and Type-II cells is developed together with the schematics of the cell wall isolation process. The review provides a critical summary of the recent trends in intact plant cells as a functional-nutritional food. It paves the way for the industrial production of intact cells as a novel food ingredient.

Extraction and characterization of proteins from banana (Musa Sapientum L) flower and evaluation of antimicrobial activities.

Ultrasonic assisted alkaline extraction of protein from banana flower was optimized using response surface methodology. The extracted proteins were characterized by Fourier transform infrared spectroscopy and molecular weight distribution was determined by gel electrophoresis. The maximum protein yield of 252.25 mg/g was obtained under optimized extraction conditions: temperature 50 °C, 30 min extraction time and 1 M NaOH concentration. The alkaline extraction produced a significantly high protein yield compared to enzymatic extraction of banana flower. Chemical finger printing of proteins showed the presence of tyrosine, tryptophan and amide bonds in extracted protein. Alkaline and pepsin assisted extracted banana flower proteins showed characteristic bands at 40 and 10 kDA, respectively. The extracted proteins showed antibacterial effects against both gram positive and gram negative bacteria. The high protein content and antimicrobial activity indicate the potential applications of banana flower in the food and feed industry.