1.
Appl Environ Microbiol
; 58(5): 1699-704, 1992 May.
Artigo
em Inglês
| MEDLINE
| ID: mdl-1622241
RESUMO
Whole cells and cell extracts of Pseudomonas putida grown in a medium containing ammonium mandelate have the capacity to produce the acyloin compound 2-hydroxypropiophenone when incubated with benzoylformate and acetaldehyde. Benzaldehyde and benzyl alcohol were formed as reaction by-products. The enantiomeric excess of the 2-hydroxypropiophenone product was found to be 91 to 92%. The absolute configuration of the enzymatically prepared product at the carbinol carbon was found to be S. The thiamine PPi-linked enzyme benzoylformate decarboxylase, purified to give a single protein band on polyacrylamide gel electrophoresis, was shown to be responsible for the catalysis of this novel condensation reaction.
Assuntos
Carboxiliases/metabolismo , Álcoois Graxos/metabolismo , Pseudomonas putida/metabolismo , Benzaldeídos/metabolismo , Biotransformação , Hidroxipropiofenona/metabolismo , Pseudomonas putida/enzimologia
2.
J Med Chem
; 35(7): 1318-20, 1992 Apr 03.
Artigo
em Inglês
| MEDLINE
| ID: mdl-1560443