Genetic incorporation of unnatural amino acids into proteins and its translational application in biomedicine / 中国药科大学学报

@#Proteins in the human body are usually made of 20 natural amino acids.Through different amino acid combinations and isomerization, proteins of diverse functions are built.An emerging genetic code expansion technology can introduce unnatural amino acids into specific sites of target protein, endowing the protein with new biological characteristics including covalently binding with proximal proteins, carrying fluorescence, and mimicking specific protein post-translational modifications.In this paper, based on the structure and function of unnatural amino acids, the applications of different types of unnatural amino acids in regulating protein''s stability, studying protein''s conformation, expression level, and localization, and uncovering heretofore unknown protein-protein interactions were reviewed.Besides, genetic code expansion of unnatural amino acids is anticipated to find broad utilities in biomedicine by bringing new ideas and methods to the design and optimization of biologics.

Improved deep learning model for differentiating novel coronavirus pneumonia and influenza pneumonia

BackgroundChest CT had high sensitivity in diagnosing novel coronavirus pneumonia (NCP) at early stage, giving it an advantage over nucleic acid detection in time of crisis. Deep learning was reported to discover intricate structures from clinical images and achieve expert-level performance in medical image analysis. To develop and validate an integrated deep learning framework on chest CT images for auto-detection of NCP, particularly focusing on differentiating NCP from influenza pneumonia (IP). Methods35 confirmed NCP cases were consecutively enrolled as training set from 1138 suspected patients in three NCP designated hospitals together with 361 confirmed viral pneumonia patients from center one including 156 IP patients, from May, 2015 to February, 2020. The external validation set enrolled 57 NCP patients and 50 IP patients from eight centers. Results96.6% of NCP lesions were larger than 1 cm and 76.8% were with intensity below -500 Hu, indicating less consolidation than IP lesions which had nodules ranging 5-10 mm. The classification schemes accurately distinguished NCP and IP lesions with area under the receiver operating characteristic curve (AUC) above 0.93. The Trinary scheme was more device-independent and consistent with specialists than the Plain scheme, which achieved a F1 score of 0.847, higher than the Plain scheme (0.774), specialists (0.785) and residents (0.644). ConclusionsOur study potentially provides an accurate early diagnosis tool on chest CT for NCP with high transferability, and shows high efficiency in differentiating NCP and IP, helping to reduce misdiagnosis and contain the pandemic transmission.

Investigation on distribution of Yersinia enterocolitica in Dengfeng f rom 2009 to 2012 / 中国人兽共患病学报

To investigate the infection of Yersinia enterocolitica in Dengfeng City ,the strains were isolated from livestock and poultry .The strains were detected with biochemiological methods ,serological methods ,and virulence genes were detected with PCR .A total of 105 strains of Yersinia enterocolitica were classified from 1 285 stool samples ,the total isolation rate was 8 .17% .Among the total isolated strains ,17 strains were classified from dogs with a rate of 17 .35% and 35 strains from pigs with 13 .62% .Twelve strains were O ∶3 serotype (13 .48% ) ,12 strains were O ∶5(13 .48% ) ,and 14 strains were O ∶8 (15 .73% ) .Ail+ ,ystA+ ,yadA+ and virF+ accounted for 12 .36% ,and ystB+ accounted for 42 .70% .In conclusion ,the pigs and dogs were important animal hosts ,which may play the major role in humans'infection .

Target Controlled or Intermittent Infusion of Sufentanil during Awake Craniotomy / 中国康复理论与实践

@#Objective To compare the efficacy and safety of the target controlled administration of sufentanil and intermittent sufentanil during awake craniotomy. Methods 40 patients were randomized into target controlled infusion of sufentanil group (n=20) and intermittent sufentanil group (n=20) and compared. Results The hemodynamic parameters were similar in the two groups. However, target controlled infusion of sufentanil was associated with significantly lower respiratory rate （P<0.05）, higher end tidal CO2 （P<0.05）, and higher dosage of sufentanil （P<0.01）, compared with those of intermittent sufentanil. Conclusion Intermittent sufentanil infusion is associated with less respiratory depression than target controlled administration of sufentanil in awake craniotomy.

Effect of Stellate Ganglion Block Plus Tianshu Capsule on Migraine / 中国康复理论与实践

@#Objective To observe the therapeutic effect of stellate ganglion block (SGB) combined with Tianshu capsule on migraine.Methods Ninty patients with migraine were randomly divided into the trial group (n=44, treated by SGB combined with Tianshu capsule) and control group (n=46, treated by SGB only). All patients of two groups were evaluated with numeric rating score (NRS) for pain before treatment and 1 day, 3 days, 1 week, 1 month and 3 months after treatment, and the therapeutic effects of two groups were compared.Results The NRS scores of patients in the two groups at each time point decreased, but scores of patients in the trial group were significantly lower than that in the control group at 3 days, 1 month and 3 months after treatment ( P<0.05). The effective rate of the trial group was superior to that of the control group ( P<0.05).Conclusion SGB combined with Tianshu capsule is more effective on migraine and has less side effect.

Effect of preoperative aprotinin on blood coagulation in patients undergoing meningioma resection / 中华麻醉学杂志

Objective To evaluate the effects of different low doses of aprotinin given before operation on blood coagulation in patients undergoing meningioma resection. Methods Sixty ASA Ⅰ or Ⅱ patients aged 18-60 yrs were randomized to receive intravenous aprotinin 0.5 or 1.0 or 2.0?106 KIU (group Apro 1, 2, 3) or normal saline (control group) before operation. 6% hydroxyethyl starch (HES) 1 000 ml was infused at 25 ml?min-1 after induction of general anesthesia. Venous blood samples were taken before aprotinin was given ( T1 , baseline), before operation after aprotinin was given (T2), while the tumor was being resected (T3), at the end of surgery (T4) and 24h after operation (T5) for routine blood and coagulation tests including Hb, Hct, RBC, WBC and PLT counts, activated partial thromboplastin time (APTT), prothrombin time (PT) fibrinogen concentration (FIB) and thrombin time (TT) and tests using Sonoclot coagulation and platelet function analyzer including Sonoclot activated clotting time (Son ACT), rate of clot formation (CR) and platelet function (PF). Results The 4 groups were comparable with respect to demographic data, duration of operation, uine output, amount of fluid infused and blood loss during operation and volume of the tumor. RBC count, Hb, Hct and PLT count were significantly decreased during and after operation (T2-5) while WBC counts were significantly increased at T5 as compared with the baseline values at T1 in the 4 groups. PT was significantly prolonged and FIB and CR significantly decreased in the 4 groups; APTT was significantly prolonged in group Apro 2 and 3; PF was significantly increased in group Apro 3 during operation at T2-4 as compared to the baseline values at T1 but all returned to normal level after operation at T5 .Conclusions Preoperative aprotinin 1.0 and 2.0?106 KIU can prolong APTT. Aprotinin 2.0?106 KIU provides effective protection of platelet function.