Assuntos
Artrópodes/microbiologia , Dirofilaria/microbiologia , Rickettsiaceae/genética , Rickettsiaceae/isolamento & purificação , Animais , Sequência de Bases , Primers do DNA/genética , DNA Bacteriano/genética , DNA Ribossômico/genética , Dirofilaria/ultraestrutura , Cães , Feminino , Masculino , Microscopia Eletrônica , Dados de Sequência Molecular , Rickettsiaceae/classificação , SimbioseRESUMO
We present serological evidence that Dirofilaria immitis was the causative agent of subcutaneous nodules in two patients. ELISA and enzyme-linked immunoblot analysis showed that the two human sera had a reactivity pattern similar to that of D. immitis rabbit immune serum. The reaction pattern of the human sera was very different to that of Dirofilaria repens rabbit immune serum. In the light of our findings, the traditional view that subcutaneous filarial nodules are due to D. repens has to be revised.
Assuntos
Dirofilaria/classificação , Dirofilariose/parasitologia , Dermatopatias Parasitárias/parasitologia , Adulto , Animais , Dirofilaria immitis/isolamento & purificação , Feminino , Humanos , Pessoa de Meia-Idade , Sorotipagem/métodosRESUMO
Sera from both clinically healthy adults (2112) and adult patients (471) were tested to assess the main risk factors for Toxocara infection in humans in Northern Italy. The patient group included 257 adult epileptics, 76 Strongyloides stercoralis-infected adult patients and 142 institutionalized mentally retarded adult patients. The overall seroprevalence in healthy population was 3.98%. No significant differences in seroprevalence were observed for sex, residence (urban or rural) or dog ownership, while seroprevalence significantly increased with age (18- greater than 51 years). Highest seroprevalence values were found in outdoor or soil-related workers. The seroprevalence was 4.35% in adult epileptics, 9.21% in Strongyloides stercoralis-infected patients and 14.47%-10.61% among institutionalized mentally retarded patients. These findings suggest that the prevalent source of human toxocariasis is the environmental contamination by infectious eggs of the parasite.
Assuntos
Toxocaríase/epidemiologia , Adolescente , Adulto , Animais , Anticorpos Anti-Helmínticos/sangue , Cães/parasitologia , Ensaio de Imunoadsorção Enzimática , Epilepsia/complicações , Fezes/parasitologia , Feminino , Humanos , Incidência , Institucionalização , Deficiência Intelectual/complicações , Itália/epidemiologia , Masculino , Pessoa de Meia-Idade , Gravidez , Complicações Infecciosas na Gravidez/epidemiologia , Prevalência , Fatores de Risco , Solo , Estrongiloidíase/complicações , Toxocara/imunologia , Toxocaríase/complicações , Toxocaríase/transmissão , ZoonosesRESUMO
Samples of heart, esophagus and diaphragm of 200 camels (Camelus dromedarius) from Southern Somalia were examined for Sarcocystis by trypsin digestion and histological examination. The results showed a very high prevalence rate (82.5%). Histological findings suggested the presence of two different species in the camel.