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1.
Mol Cell Biol ; 20(17): 6308-16, 2000 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-10938107

RESUMO

Heterochromatin is characteristically the last portion of the genome to be replicated. In polytene cells, heterochromatic sequences are underreplicated because S phase ends before replication of heterochromatin is completed. Truncated heterochromatic DNAs have been identified in polytene cells of Drosophila and may be the discontinuous molecules that form between fully replicated euchromatic and underreplicated heterochromatic regions of the chromosome. In this report, we characterize the temporal pattern of heterochromatic DNA truncation during development of polytene cells. Underreplication occurred during the first polytene S phase, yet DNA truncation, which was found within heterochromatic sequences of all four Drosophila chromosomes, did not occur until the second polytene S phase. DNA truncation was correlated with underreplication, since increasing the replication of satellite sequences with the cycE(1672) mutation caused decreased production of truncated DNAs. Finally, truncation of heterochromatic DNAs was neither quantitatively nor qualitatively affected by modifiers of position effect variegation including the Y chromosome, Su(var)205(2), parental origin, or temperature. We propose that heterochromatic satellite sequences present a barrier to DNA replication and that replication forks that transiently stall at such barriers in late S phase of diploid cells are left unresolved in the shortened S phase of polytene cells. DNA truncation then occurs in the second polytene S phase, when new replication forks extend to the position of forks left unresolved in the first polytene S phase.


Assuntos
Cromossomos/ultraestrutura , Drosophila/genética , Heterocromatina/genética , Heterocromatina/fisiologia , Animais , Núcleo Celular/ultraestrutura , Separação Celular , Homólogo 5 da Proteína Cromobox , Cruzamentos Genéticos , Ciclina E/genética , DNA/genética , Diploide , Proteínas de Drosophila , Citometria de Fluxo , Modelos Genéticos , Mutação , Ploidias , Fase S , Fatores de Tempo , Cromossomo Y/ultraestrutura
2.
Int Arch Allergy Appl Immunol ; 86(2): 238-42, 1988.
Artigo em Inglês | MEDLINE | ID: mdl-3391689

RESUMO

Mice immunized with rabbit renal basement membranes form autoantibodies to their kidney glomerular and tubular basement membranes (GBM/TBM). Development of renal tubular disease (RTD) consists of deposition of autoantibodies along the GBM/TBM with the inter- and intratubular accumulation of lymphocytes and macrophages and destruction of the TBM. Transfer of this disease in mice with either serum or monoclonal antibodies, however, has been difficult to demonstrate and, therefore, attempts were made to confirm a report that RTD is passively transferred by anti-TBM autoantibodies. Using the revised protocol in this later report, we found that 12 weeks after transfer autoantibodies were deposited along the GBM and/or TBM of the recipients, yet RTD was not observed. Although qualitative and quantitative characteristics of the antibody may play a role in the pathogenesis in the murine model of RTD, we could not obtain evidence to support and confirm this study.


Assuntos
Anticorpos Monoclonais/administração & dosagem , Antígenos/imunologia , Autoantígenos/imunologia , Imunização Passiva , Nefrite Intersticial/imunologia , Animais , Especificidade de Anticorpos , Membrana Basal/imunologia , Cobaias , Imunização Passiva/métodos , Glomérulos Renais/imunologia , Túbulos Renais/imunologia , Camundongos , Camundongos Endogâmicos , Nefrite Intersticial/sangue
4.
Am J Pathol ; 116(1): 107-14, 1984 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-6377906

RESUMO

Lupus-prone (NZB X NZW)F1 (B X W) mice and MRL-lpr and BXSB mice were examined for the prevalence of hypertension and levels of plasma renin activity (PRA). Hypertension (greater than 145 mmHg) was observed only in female and male B X W mice with severe nephritis; in female MRL-lpr and male BXSB mice severe nephritis developed without blood pressure elevation (80-135 mmHg). The B X W parental strains, NZB and NZW, and the MRL-lpr congenic partners, MRL- +, did not become hypertensive as they aged. Other strains of mice, aged 3-32 months (A/HeN, BALB/cJ, BALB/cByJ, B10.S/Sg, B10.D2/ oSn , CBA/J, C3H/HeJ, SJL/J and [SJL X NZW]F1), also had normal blood pressure (98-122 mmHg). All mice with lupus nephritis had low PRA, even those with hypertension; furthermore, the MRL-lpr strain had low or undetectable PRA (2 +/- 1 ng/ml/hr), even when kidneys were normal. NZB, NZW, and MRL- + mice had normal PRA (10-16 ng/ml/hr). Thus, B X W mice frequently developed low renin hypertension during the last phase of their renal disease; whereas MRL-lpr and BXSB mice died from renal disease without observable increases in blood pressure.


Assuntos
Glomerulonefrite/complicações , Hipertensão/genética , Lúpus Eritematoso Sistêmico/complicações , Animais , Pressão Sanguínea , Feminino , Glomerulonefrite/patologia , Hibridização Genética , Hipertensão/epidemiologia , Rim/patologia , Masculino , Camundongos , Camundongos Endogâmicos BALB C/genética , Camundongos Endogâmicos C3H/genética , Camundongos Endogâmicos CBA/genética , Camundongos Endogâmicos NZB/genética , Renina/sangue , Especificidade da Espécie
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