RESUMO
The polyoma virus region expressed early in the lytic cycle encodes three proteins, or T-antigens, that together cause the infected cell to enter the cell cycle and so provide a suitable cellular environment for replication of the viral genome. Under some circumstances infection does not kill the cell, but the T-antigens are still produced, resulting in the cell becoming transformed and tumorigenic. Most of this transforming action is exerted by the middle T-antigen, which has the ability to convert established cell lines to an oncogenic state. Middle T is a membrane bound polypeptide that interacts with a number of the proteins used by tyrosine kinase associated receptors to stimulate mitogenesis, so MT can be considered as a permanently active analogue of a receptor. Through a defined series of interactions, MT assembles a large multi-protein complex at the cell membrane, consisting of MT, the core dimer of protein phosphatase 2A, an src-family tyrosine kinase, and via phosphotyrosines, ShcA, phosphatidylinositol (3') kinase, and phospholipase Cgamma-1. Tyrosine phosphorylation stimulates PI3K and PLCgamma-1 enzymatic activity, and on ShcA creates binding sites for Grb2 with its associated Sos1 and Gab1. This activates p21(ras), and hence, the MAP kinase cascade. Consequently, MT can be used as a model for studying cell transformation and growth factor receptor signalling pathways.
Assuntos
Antígenos Transformantes de Poliomavirus/imunologia , Transformação Celular Viral/imunologia , Polyomavirus/imunologia , Animais , HumanosRESUMO
ShcA and Grb2 are crucial components in signalling by most tyrosine kinase-associated receptors. How ever, it is not clear whether Grb2 bound directly to the receptor is equivalent to Grb2 associated via ShcA. We have used signalling stimulated by the middle T-antigen (MT) of polyoma virus to address this question. The two known Grb2-binding sites from murine ShcA, 313Y and 239/240YY, could functionally replace the MT ShcA-interacting region in transformation assays using Rat2 fibroblasts. This demonstrates that signal output from membrane-bound ShcA requires only these two sequences and the ShcA-binding site in MT does not recruit other signalling molecules. Two standard Grb2-interacting sequences, either from the EGF receptor or the ShcA 313Y region, could not replace the requirement for ShcA binding to MT, indicating an enhanced role for the ShcA 239/240YY motif. Sos1 and the docking protein Gab1 are brought into the MT complex through Grb2 association and this may be more effective using the 239/240YY sequence.
Assuntos
Proteínas Adaptadoras de Transdução de Sinal , Proteínas/metabolismo , Tirosina/metabolismo , Motivos de Aminoácidos , Sequência de Aminoácidos , Animais , Sítios de Ligação , Western Blotting , Membrana Celular/metabolismo , Receptores ErbB/metabolismo , Fibroblastos/metabolismo , Proteína Adaptadora GRB2 , Camundongos , Dados de Sequência Molecular , Fosfoproteínas/metabolismo , Fosforilação , Plasmídeos/metabolismo , Testes de Precipitina , Ligação Proteica , Ratos , Proteína SOS1/metabolismo , Homologia de Sequência de Aminoácidos , Proteínas Adaptadoras da Sinalização Shc , Transdução de Sinais , Proteína 1 de Transformação que Contém Domínio 2 de Homologia de Src , Domínios de Homologia de srcRESUMO
The Raf-1 kinase plays a key role in relaying proliferation signals elicited by mitogens or oncogenes. Raf-1 is regulated by complex and incompletely understood mechanisms including phosphorylation. A number of studies have indicated that phosphorylation of serines 259 and 621 can inhibit the Raf-1 kinase. We show that both serines are hypophosphorylated during early mitogenic stimulation and that hypophosphorylation correlates with peak Raf-1 activation. Concentrations of okadaic acid that selectively inhibit protein phosphatase 2A (PP2A) induce phosphorylation of these residues and prevent maximal activation of the Raf-1 kinase. This effect is mediated via phosphorylation of serine 259. The PP2A core heterodimer forms complexes with Raf-1 in vivo and in vitro. These data identify PP2A as a positive regulator of Raf-1 activation and are the first indication that PP2A may support the activation of an associated kinase.
Assuntos
Fosfoproteínas Fosfatases/metabolismo , Proteínas Proto-Oncogênicas c-raf/metabolismo , Animais , Células Cultivadas , Ativação Enzimática , Proteína Fosfatase 2 , Transdução de SinaisRESUMO
Polymoma virus encodes a potent oncogene, the middle T-antigen (MT), that induces cell transformation by copying the actions of tyrosine kinase associated growth factor receptors. A crucial component of MT transformation is its ability to bind and stimulate the activity of src-family kinases. However, the mechanism by which this is achieved remains unclear. Tyrosine phosphorylation of MT by src-kinases then provides binding sites for SH2 and PTB domain containing molecules in a paradigm of receptor action. We present evidence here that the MT/src complex contains equi-molar amounts of PP2A, and that phosphatase activity may be required for the interaction of MT with both PP2A and the src-family. PP2A, then, is a necessary component of the MT-src complex. We also show that two motifs in the 185 to 210 region of MT, each consisting of a basic area followed by a serine or threonine, are essential for interaction with src-kinases, but not PP2A. The spacing between the serine or threonine and the basic sequence also appears to be important. Substituting a cysteine residue in place of Thr203 in MT has no affect on the binding of pp60c-src, showing that these sites interact with src-kinases by a novel mechanism that does not require phosphorylation.
Assuntos
Antígenos Transformantes de Poliomavirus/metabolismo , Fosfoproteínas Fosfatases/fisiologia , Quinases da Família src/metabolismo , Sequência de Aminoácidos , Animais , Linhagem Celular , Modelos Biológicos , Dados de Sequência Molecular , Mutagênese Sítio-Dirigida , Fosforilação , Proteínas Proto-Oncogênicas pp60(c-src)/metabolismo , Ratos , Homologia de Sequência de Aminoácidos , Fatores de TempoRESUMO
Stimulation of the T cell antigen receptor (TCR) triggers a complex series of signaling events that culminate in T cell activation and proliferation. The complex structure of the TCR has hindered efforts to link specific signaling events induced by TCR cross-linkage to downstream activation responses, such as interleukin-2 (IL-2) gene transcription. Previous studies have shown that the polyomavirus-derived oncoprotein, middle T antigen (mT), transforms rodent fibroblasts by interacting with and activating several cytoplasmic signaling proteins (Src kinases, phospholipase C (PLC)-gamma1, Shc, and phosphoinositide 3-kinase (PI3-K) implicated in cell growth control. In this study, we demonstrate that expression of mT activates Jurkat T cells, as measured by increases in IL-2 promoter- and NFAT (nuclear factor of activated T cells)-dependent reporter gene transcription. The transcriptional response provoked by mT was blocked by the immunosuppressive drug FK506, a potent inhibitor of TCR-mediated IL-2 gene expression. Mutations that disrupted the binding of mT to Src kinases or PLC-gamma1 abrogated the ability of mT to deliver the signals needed for IL-2 promoter activation. In contrast, a mT mutant that failed to bind PI3-K induced a markedly elevated transcriptional response in Jurkat cells, whereas mutation of the Shc binding site in mT had little effect on the transactivating potential of this viral oncoprotein. Additional studies demonstrated that the association of mT with PLC-gamma1 was necessary and sufficient to activate both Ca2+- and Ras-dependent signaling cascades in Jurkat cells. These results indicate that PLC-gamma1 activation plays pivotal and pleiotropic roles in the stimulation of IL-2 gene expression, whereas activation of PI3-K negatively modulates this response in Jurkat T cells.
Assuntos
Antígenos Transformantes de Poliomavirus , Receptores de Antígenos de Linfócitos T/fisiologia , Androstadienos/farmacologia , Calcineurina/fisiologia , Cálcio/fisiologia , Proteínas Quinases Dependentes de Cálcio-Calmodulina/metabolismo , Inibidores Enzimáticos/farmacologia , Regulação da Expressão Gênica , Humanos , Interleucina-2/genética , Isoenzimas/fisiologia , Células Jurkat , Inibidores de Fosfoinositídeo-3 Quinase , Fosfolipase C gama , Transdução de Sinais , Tacrolimo/farmacologia , Transcrição Gênica , Transfecção , Fosfolipases Tipo C/fisiologia , WortmaninaRESUMO
The phosphotyrosine interaction (PI)/phosphotyrosine binding (PTB) domain of Shc binds specific tyrosine-phosphorylated motifs found on activated growth factor receptors and proteins such as polyoma virus middle T antigen (MT). Phenylalanine 198 (Phe198) has been identified as a crucial residue involved in the interaction of the Shc PI/PTB with phosphopeptides. In NIH 3T3 cells expressing MT, p52 Shc carrying the F198V mutation is weakly phosphorylated and does not bind MT or Grb2. Overexpression of the PI/PTB domain alone as Shc amino acids 1-238 acted in a dominant interfering fashion blocking MT-induced transformation. However, expression of a slightly longer construct, Shc 1-260, which encompasses Tyr239/Tyr240, a novel Shc tyrosine phosphorylation site, did not block transformation. This was found to be due to the ability of Shc 1-260 to become tyrosine-phosphorylated and bind Grb2. Furthermore, full-length Shc in which Tyr239/Tyr240 had been mutated to phenylalanine did not become tyrosine-phosphorylated or bind Grb2 but did inhibit colony formation in soft agar. Conversely, p52 Shc carrying a mutation in the other tyrosine phosphorylation site, Tyr317, became heavily tyrosine-phosphorylated, bound Grb2, and gave rise to colonies in soft agar.
Assuntos
Proteínas Adaptadoras de Transdução de Sinal , Proteínas Adaptadoras de Transporte Vesicular , Antígenos Transformantes de Poliomavirus/fisiologia , Transformação Celular Neoplásica , Fosfotirosina/fisiologia , Proteínas/fisiologia , Transdução de Sinais , Tirosina/metabolismo , Células 3T3 , Sequência de Aminoácidos , Animais , Sítios de Ligação , Proteína Adaptadora GRB2 , Camundongos , Dados de Sequência Molecular , Fosforilação , Proteínas/química , Proteínas/metabolismo , Proteínas Adaptadoras da Sinalização Shc , Proteína 1 de Transformação que Contém Domínio 2 de Homologia de SrcRESUMO
Interaction with the src family of tyrosine kinases is crucial to the transforming action of polyomavirus middle T-antigen (MT). Association with MT activates the tyrosine kinase activity of pp60(c-src) and, through subsequent MT phosphorylation, creates binding sites for signalling molecules whose stimulation culminates in cell transformation. Despite this importance, and many studies, little is known of the mechanisms by which pp60(c-src) binds to MT. We report here isolation of the first MT mutants that disrupt pp60(c-src) binding without affecting the interaction between MT and protein phosphatase 2A (PP2A). Through deletion analysis we established that interaction with pp60(c-src) requires the sequences between amino acids 185 and 210 of MT, but these residues have no effect on PP2A binding. Cells expressing these mutants showed few altered properties, indicating that the PP2A-MT interaction alone has little influence on cell phenotype. Subcellular location of these mutant MT molecules was indistinguishable by immunofluorescence analysis from that of wild-type MT but was altered markedly on loss of PP2A binding. This suggests a possible role for PP2A in specifying subcellular distribution.
Assuntos
Antígenos Transformantes de Poliomavirus/metabolismo , Proteínas Proto-Oncogênicas pp60(c-src)/metabolismo , Sequência de Aminoácidos , Animais , Antígenos Transformantes de Poliomavirus/genética , Linhagem Celular , Dados de Sequência Molecular , Mutação , Fenótipo , Fosfatidilinositol 3-Quinases , Fosfotransferases (Aceptor do Grupo Álcool)/metabolismo , Ratos , Relação Estrutura-Atividade , Transformação GenéticaRESUMO
Polyoma virus middle T antigen duplicates the actions of growth-factor receptors in binding the signalling molecules phosphatidylinositol 3'-OH kinase and Shc. These properties indicate that middle T is mitogenic and may be required to overcome inhibition of DNA replication during the lytic life cycle of the virus.
Assuntos
Antígenos Virais de Tumores/fisiologia , Infecções por Papillomavirus/virologia , Polyomavirus/imunologia , Infecções Tumorais por Vírus/virologia , Animais , Antígenos Virais de Tumores/genética , Antígenos Virais de Tumores/metabolismo , Evolução Biológica , Camundongos , Infecções por Papillomavirus/imunologia , Ligação Proteica , Receptores de Fatores de Crescimento/metabolismo , Infecções Tumorais por Vírus/imunologia , Proteínas Virais/metabolismoRESUMO
Polyoma virus middle T-antigen converts normal fibroblasts to a fully transformed, tumorigenic phenotype. It achieves this, at least in part, by binding and activating one of the non-receptor tyrosine kinases, pp60c-src, pp62c-yes or pp59c-fyn (reviewed in refs 2 and 3). As a result, middle T-antigen itself is phosphorylated on tyrosine residues, one of which (Tyr 315) acts as a binding site for the SH2 domains of phosphatidylinositol-3'OH kinase 85K subunit. Here we show that another tyrosine phosphorylation site in middle T-antigen (Tyr 250; refs 4, 5) acts as a binding region for the SH2 domain of the transforming protein Shc. This results in Shc also becoming tyrosine-phosphorylated and binding to the SH2 domain of Grb2 (ref. 10). This probably stimulates p21ras activity through the mammalian homologue of the Drosophila guanine-nucleotide-exchange factor Sos (reviewed in ref. 11). We suggest that middle T-antigen transforms cells by acting as a functional homologue of an activated tyrosine kinase-associated growth-factor receptor.
Assuntos
Proteínas Adaptadoras de Transdução de Sinal , Proteínas Adaptadoras de Transporte Vesicular , Antígenos Transformantes de Poliomavirus/metabolismo , Transformação Celular Neoplásica , Transformação Celular Viral , Proteínas/metabolismo , Tirosina/metabolismo , Sequência de Aminoácidos , Animais , Antígenos Transformantes de Poliomavirus/genética , Linhagem Celular , Proteína Adaptadora GRB2 , Dados de Sequência Molecular , Mutação , Fosfatidilinositol 3-Quinases , Fosforilação , Fosfotransferases (Aceptor do Grupo Álcool)/metabolismo , Ligação Proteica , Proteínas Proto-Oncogênicas pp60(c-src)/metabolismo , Ratos , Receptores Proteína Tirosina Quinases/metabolismo , Receptores de Fatores de Crescimento de Fibroblastos/metabolismo , Proteínas Adaptadoras da Sinalização Shc , Proteína 1 de Transformação que Contém Domínio 2 de Homologia de SrcRESUMO
Fourteen pGEX plasmids that express defined regions of polyomavirus middle T antigen in bacteria have been constructed. These polypeptides have been used to generate 18 new monoclonal antibodies directed against the unique portion of middle T and to map the approximate position of the antibody recognition sites onto the protein sequence. All of the antibodies effectively immunoprecipitate middle T and the associated 60- and 35-kDa components of protein phosphatase 2A. Four of the antibodies, however, do not react with middle T when it is bound to pp60c-src. These four probably bind to amino acids 203 to 218 of the middle T protein sequence, which are encoded by the mRNA immediately 3' to the splice junction that creates the C-terminal unique region. This suggests that additional middle T sequences are required for middle T's interaction with pp60c-src than are needed for its binding to protein phosphatase 2A. The antibodies localize this extra region and provide a means of distinguishing between these two associations.
Assuntos
Anticorpos Monoclonais/imunologia , Anticorpos Antivirais/imunologia , Antígenos Transformantes de Poliomavirus/imunologia , Fosfoproteínas Fosfatases/metabolismo , Polyomavirus/imunologia , Proteínas Proto-Oncogênicas pp60(c-src)/metabolismo , Sequência de Aminoácidos , Animais , Antígenos Transformantes de Poliomavirus/metabolismo , Sequência de Bases , Células Cultivadas , Clonagem Molecular , Epitopos , Técnicas In Vitro , Camundongos , Dados de Sequência Molecular , Peso Molecular , Oligodesoxirribonucleotídeos/química , Fragmentos de Peptídeos/imunologia , Fosfoproteínas/química , Fosfoproteínas/metabolismo , Ligação Proteica , Proteínas Quinases/metabolismo , Proteína Fosfatase 2 , Alinhamento de SequênciaRESUMO
The polyomavirus minor late capsid antigen, VP2, is myristylated on its N-terminal glycine, this modification being required for efficient infection of mouse cells. To study further the functions of this antigen, as well as those of the other minor late antigen, VP3, recombinant baculoviruses carrying genes for VP1, VP2, and VP3 have been constructed and the corresponding proteins have been synthesized in insect cells. A monoclonal antibody recognizing VP1, alpha-PyVP1-A, and two monoclonal antibodies against the common region of VP2 and VP3, alpha-PyVP2/3-A and alpha-PyVP2/3-B, have been generated. Reactions of antibodies with antigens were characterized by indirect immunofluorescence, immunoprecipitation, and immunoblot analysis. Immunofluorescent staining of mouse cells infected with polyomavirus showed all antigens to be localized in nuclei. When the late polyomavirus proteins were expressed separately in insect cells, however, only VP1 was efficiently transported into the nucleus; VP2 was localized discretely around the outside of the nucleus, and VP3 exhibited a diffused staining pattern in the cytoplasm. Coexpression of VP2, or VP3, with VP1 restored nuclear localization. Immunoprecipitation of infected mouse cells with either anti-VP1 or anti-VP2/3 antibodies precipitated complexes containing all three species, consistent with the notion that VP1 is necessary for efficient transport of VP2 and VP3 into the nucleus. Purified empty capsid-like particles, formed in nuclei of insect cells coinfected with all three baculoviruses, contained VP2 and VP3 proteins in amounts comparable to those found in empty capsids purified from mouse cells infected with wild-type polyomavirus. Two-dimensional gel analysis of VP1 species revealed that coexpression with VP2 affects posttranslational modification of VP1.
Assuntos
Antígenos Virais/metabolismo , Polyomavirus/crescimento & desenvolvimento , Proteínas Estruturais Virais/metabolismo , Sequência de Aminoácidos , Animais , Anticorpos Antivirais , Antígenos Virais/genética , Antígenos Virais/imunologia , Baculoviridae/genética , Transporte Biológico , Western Blotting , Capsídeo/biossíntese , Capsídeo/genética , Capsídeo/imunologia , Capsídeo/isolamento & purificação , Capsídeo/ultraestrutura , Proteínas do Capsídeo , Compartimento Celular , Núcleo Celular/química , Células Cultivadas , Citoplasma/química , Hibridomas , Dados de Sequência Molecular , Polyomavirus/genética , Polyomavirus/ultraestrutura , Testes de Precipitina , Proteínas Estruturais Virais/genética , Proteínas Estruturais Virais/imunologiaRESUMO
Nucleoplasmin is the most abundant nuclear protein in Xenopus oocytes and eggs. The term 'molecular chaperone' was coined to describe its role in the assembly of the nucleosome subunits of chromatin. Although histones and DNA can self-assemble into nucleosomes, nucleoplasmin can facilitate this process in vitro by competing against non-specific charge interactions. In vivo nucleoplasmin binds histones H2A and H2B and transfers them to DNA. Another acidic nuclear protein, N1, binds and transfers histones H3 and H4. Nucleoplasmin has at least one other role in modulating chromatin structure in Xenopus eggs. It is required for the first stage of sperm chromatin decondensation. It binds and removes sperm basic proteins and replaces them by histones H2A and H2B, again forming nucleosomes, and resulting in decondensation of the compacted sperm chromatin. In addition we propose that the properties of the nuclear localization signal of nucleoplasmin can be explained by a model in which heat shock cognate protein hsc70 has a chaperone role in signal presentation during nuclear transport.
Assuntos
Cromatina/fisiologia , Proteínas Nucleares/metabolismo , Fosfoproteínas , Sequência de Aminoácidos , Animais , Chaperoninas , Feminino , Histonas/metabolismo , Humanos , Dados de Sequência Molecular , Nucleoplasminas , Oócitos/fisiologia , Óvulo/fisiologia , Proteínas/metabolismo , Homologia de Sequência de Aminoácidos , XenopusRESUMO
A monoclonal antibody specific for Xenopus MyoD (XMyoD) has been characterized and used to describe the pattern of expression of this myogenic factor in early frog development. The antibody recognizes an epitope close to the N terminus of the products of both XMyoD genes, but does not bind XMyf5 or XMRF4, the other two myogenic factors that have been described in Xenopus. It reacts in embryo extracts only with XMyoD, which is extensively phosphorylated in the embryo. The distribution of XMyoD protein, seen in sections and whole-mounts, and by immunoblotting, closely follows that of XMyoD mRNA. XMyoD protein accumulates in nuclei of the future somitic mesoderm from the middle of gastrulation. In neurulae and tailbud embryos it is expressed specifically in the myotomal cells of the somites. XMyoD is in the nucleus of apparently every cell in the myotomes. It accumulates first in the anterior somitic mesoderm, and its concentration then declines in anterior somites from the tailbud stage onwards.
Assuntos
Núcleo Celular/química , Proteínas de Ligação a DNA/análise , Gástrula/química , Proteínas Musculares/análise , Músculos/embriologia , Animais , Anticorpos Monoclonais , Immunoblotting , Imuno-Histoquímica , Mesoderma/química , Proteínas Musculares/imunologia , Proteína MyoD , Testes de Precipitina , Xenopus laevisRESUMO
An antibody that recognizes the phosphorylated form of nucleoplasmin has identified another nuclear protein whose antigenic form is regulated in a mitosis-specific manner, with a dramatic increase in binding occurring in all mitotic cells. The protein is localised around the periphery of condensed chromosomes during mitosis in a manner analogous to another nucleoplasmin-related polypeptide NO38. Mitosis-specific expression of the antigenic site is dependent on phosphorylation of the polypeptide; binding of the antibody is dramatically reduced by prior incubation of the polypeptide with phosphatases. Migration on SDS-PAGE suggests that the protein has an exceptionally large relative molecular mass, in excess of 400,000. The probable mitosis-specific phosphorylation and location of this antigen suggests a subcellular storage mechanism for proteins during mitosis.
Assuntos
Núcleo Celular/química , Cromossomos/química , Mitose , Proteínas Nucleares/análise , Fosfoproteínas , Animais , Anticorpos Monoclonais , Linhagem Celular , Reações Cruzadas , Eletroforese em Gel de Poliacrilamida , Peso Molecular , Proteínas Nucleares/química , Proteínas Nucleares/imunologia , Proteínas Nucleares/metabolismo , Nucleoplasminas , Monoéster Fosfórico Hidrolases/metabolismo , Fosforilação , Xenopus laevisRESUMO
Point mutagenesis of the nuclear targeting sequence of nucleoplasmin has identified two interdependent basic domains. These are separated by 10 intervening "spacer" amino acids that tolerate point mutations and some insertions. Amino acids in both basic domains are required for nuclear targeting, and the transport defect of a mutation in one domain is amplified by a simultaneous mutation in the other. Therefore, these basic domains are interdependent. A strikingly similar motif of two clusters of basic residues is seen in the nuclear targeting sequence of Xenopus N1. It is also conserved in the related nucleolar protein NO38. Several other short sequences known to be necessary for nuclear targeting fall within a similar motif.
Assuntos
Proteínas Nucleares/metabolismo , Fosfoproteínas , Sequência de Aminoácidos , Animais , Proteína Quinase CDC2/metabolismo , Compartimento Celular , Núcleo Celular/metabolismo , Análise Mutacional de DNA , Imunofluorescência , Dados de Sequência Molecular , Nucleoplasminas , Piruvato Quinase/genética , Piruvato Quinase/metabolismo , Relação Estrutura-Atividade , Xenopus laevisRESUMO
The large T-antigens of SV40 and polyoma virus are nuclear, multifunctional proteins that are essential for replication of the respective viruses. They can also 'transform' cells in culture to varying extents; both can immortalise primary cells, and SV40 large T can additionally induce full transformation. Recently, p105Rb, the protein product of the anti-oncogene RB-1, has been shown to interact with both large T-antigens. SV40 large T-antigen also binds to a p105Rb related protein, p107. SV40 large T differs from that of polyoma virus in its ability to associate with another anti-oncogene product, p53. The significance of these properties to the transforming potential of both viruses is considered.
Assuntos
Antígenos Virais de Tumores/fisiologia , Transformação Celular Viral/fisiologia , Polyomavirus/fisiologia , Vírus 40 dos Símios/fisiologia , Antígenos Virais de Tumores/genética , Transformação Celular Viral/genética , Transformação Celular Viral/imunologia , Humanos , Polyomavirus/genética , Polyomavirus/imunologia , Vírus 40 dos Símios/genética , Vírus 40 dos Símios/imunologiaRESUMO
Each subunit of the Xenopus nucleoplasmin polypeptide possesses a single nuclear location sequence, the boundaries of which have been determined by deletion analysis. However, the sequence identified in this analysis is unable to locate pyruvate kinase to the cell nucleus. Further investigation revealed that while this sequence element is not sufficient for nuclear localisation, it is a necessary feature of slightly longer sequences which can locate pyruvate kinase to the cell nucleus. This unusual feature of the nucleoplasmin sequence suggests testable models for the interaction of this sequence with the transport mechanism, one of which is discussed here.
Assuntos
Proteínas Nucleares , Fosfoproteínas , Sinais Direcionadores de Proteínas/análise , Sequência de Aminoácidos , Animais , Deleção Cromossômica , Modelos Biológicos , Dados de Sequência Molecular , Nucleoplasminas , XenopusRESUMO
The carboxy-terminal tail of nucleoplasmin, which specifies entry into the cell nucleus, contains four short sequences that are similar to previously identified nuclear location sequences. We show that none of these is able to locate chicken muscle pyruvate kinase to the cell nucleus. Deletion analysis was used to determine the limits of a nuclear location sequence and indicated that a 14-amino acid segment (RPAATKKAGQAKKK) should function as a minimal nuclear location sequence. When tested directly, however, this sequence was unable to locate pyruvate kinase to the cell nucleus. Restoration of three amino acids of nucleoplasmin sequence at either end of this sequence generated sequences that were able to locate pyruvate kinase to the cell nucleus. The 14-amino acid proposed minimal nuclear location sequence is present in the functional sequences, AVKRPAATKKAGQAKKK, RPAATKKAGQAKKKKLD, and the sequence AVKRPAATKKAGQAKKKKLD, which has additional amino acids at both ends. The minimal sequence element is therefore necessary but not sufficient for transport into the cell nucleus. This unusual feature of the nucleoplasmin nuclear location sequence suggests ways in which it could interact with the nuclear transport mechanism.
Assuntos
Antígenos Transformantes de Poliomavirus/genética , Proteínas Nucleares/genética , Fosfoproteínas , Sequência de Aminoácidos , Animais , Antígenos Transformantes de Poliomavirus/análise , Núcleo Celular/análise , Núcleo Celular/metabolismo , Deleção Cromossômica , DNA/análise , Vetores Genéticos , Microinjeções , Dados de Sequência Molecular , Proteínas Nucleares/análise , Nucleoplasminas , Plasmídeos , Piruvato Quinase/genética , Células VeroRESUMO
When injected into the cytoplasm of Vero cells, nucleoplasmin rapidly concentrates in a narrow layer around the nuclear envelope and then accumulates within the nucleus. Transport into the nucleus can be reversibly arrested at the perinuclear stage by metabolic inhibitors or by chilling. Nucleoplasmin-coated colloidal gold particles concentrate around the nuclear envelope of Vero cells or Xenopus oocytes, and by electron microscopy of oocytes appear to be associated with fibrils attached to nuclear pore complexes. Perinuclear accumulation is not observed for the nonmigrating nucleoplasmin core fragment or nonnuclear proteins. We propose two steps in nuclear migration of proteins: rapid binding around the nuclear envelope, possibly to pore-associated fibrils, followed by slower, energy-dependent translocation through nuclear pores.