Your browser doesn't support javascript.
loading
Mostrar: 20 | 50 | 100
Resultados 1 - 13 de 13
Filtrar
Mais filtros











Base de dados
Intervalo de ano de publicação
1.
Maedica (Bucur) ; 18(1): 67-73, 2023 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-37266477

RESUMO

Aim: The evolution of coagulation factors in pregnant patients infected with SARS-CoV-2 during the pandemic is still debated. However, few studies have been carried out to evaluate the silent alterations of blood values in mild forms of the disease. Methods:A total of 153 pregnant patients with an asymptomatic form of COVID-19 and 306 healthy pregnant patients, who were admitted for delivery in our hospital between April 1, 2020 and March 1, 2022, were studied. The blood values harvested closest to the time of delivery were considered. Results:There was a significant variability in values of fibrinogen, prothrombin time, though these were still within normal limits. Conclusions:Pregnant patients with mild forms of COVID-19 displayed some blood alterations, even if they were asymptomatic for COVID-19.

2.
Molecules ; 28(6)2023 Mar 18.
Artigo em Inglês | MEDLINE | ID: mdl-36985729

RESUMO

Heparin (Hep), with its anticoagulant activity, antiangiogenic and apoptotic effects, and growth factor binding, plays an important role in various biological processes. Formulations as drug delivery systems protect its biological activity, and limit the potential side effects of faulty administration. The objective of this study was to develop novel xanthan-based materials as a delivery carrier for heparin. The materials exhibited remarkable elastic behavior and toughness without any crack development within the network, which also support their application for tissue engineering. It was found that all materials possessed the ability to control the release of heparin, according to the Korsmeyer-Peppas release model. All Hep-containing materials caused significant exchanges of the activated partial thromboplastin time (aPTT) and prothrombin time (PT) parameters, indicating that formulated natural/natural synthetic polymeric networks conserved heparin's biological activity and its ability to interrupt the blood coagulation cascade. The obtained results confirmed that developed materials could be carriers for the controlled release of heparin, with potential applications in topical administration.


Assuntos
Heparina , Polissacarídeos Bacterianos , Heparina/farmacologia , Tempo de Tromboplastina Parcial , Testes de Coagulação Sanguínea , Anticoagulantes/farmacologia
3.
Biomedicines ; 11(2)2023 Feb 08.
Artigo em Inglês | MEDLINE | ID: mdl-36831025

RESUMO

Abdominal fat and fat-free masses report a close association with cardiometabolic risks, therefore this specific body compartment presents more interest than whole-body masses. This research aimed to develop accurate algorithms that predict body masses and specifically trunk fat and fat-free masses from easy to measure parameters in any setting. The study included 104 apparently healthy subjects, but with a higher-than-normal percent of adiposity or waist circumference. Multiple linear regression (MLR) and artificial neural network (ANN) models were built for predicting abdominal fat and fat-free masses in patients with relatively low cardiometabolic risks. The data were divided into training, validation and test sets, and this process was repeated 20 times per each model to reduce the bias of data division on model accuracy. The best performance models used a maximum number of five anthropometric inputs, with higher R2 values for ANN models than for MLR models (R2 = 0.96-0.98 vs. R2 = 0.80-0.94, p = 0.006). The root mean square error (RMSE) for all predicted parameters was significantly lower for ANN models than for MLR models, suggesting a higher accuracy for ANN models. From all body masses predicted, trunk fat mass and fat-free mass registered the best performance with ANN, allowing a possible error of 1.84 kg for predicting the correct trunk fat mass and 1.48 kg for predicting the correct trunk fat-free mass. The developed algorithms represent cost-effective prediction tools for the most relevant adipose and lean tissues involved in the physiopathology of cardiometabolic risks.

4.
Metabolites ; 12(12)2022 Dec 04.
Artigo em Inglês | MEDLINE | ID: mdl-36557255

RESUMO

This research focused on establishing a hierarchy concerning the influence of various biological markers and body composition parameters on preventing, diagnosing and managing Metabolic Syndrome (MetS). Our cross-sectional cohort study included 104 subjects without any atherosclerotic antecedent pathology, organized in two groups (with and without MetS). All participants underwent clinical and anthropometric measurements, DEXA investigation and blood tests for all MetS criteria, together with adiponectin, leptin, insulin, uric acid and CRP. Based on mathematical logic, we calculated a normalized sensitivity score to compare the predictive power of biomarkers and parameters associated with MetS, upon the prevalence of MetS. Patients with MetS report higher levels of uric acid (p = 0.02), CRP (p = 0.012) and lower levels of adiponectin (p = 0.025) than patients without MetS. The top three biological markers with the highest predictive power of the prevalence of the disease are HDL, insulin, and adiponectin:leptin ratio, and the top three body composition parameters are trunk fat-free percentage, waist-height ratio and trunk fat percentage. Their high sensitivity scores differentiate them from all the other markers analysed in the study. Our findings report relevant scores for estimating the importance of cardiometabolic risks in the prevalence of MetS. The high rank of protective markers, HDL and trunk fat-free percentage, suggest that positive effects have a stronger association with the prevalence of MetS, than negative ones do. Therefore, this risk stratification study provides important support for prevention and management programs regarding MetS.

5.
Healthcare (Basel) ; 10(4)2022 Mar 25.
Artigo em Inglês | MEDLINE | ID: mdl-35455798

RESUMO

BACKGROUND: The present study aimed to investigate the association of obesity phenotypes and quality of life (QoL) scales and their relationship with fat mass (FM) parameters. METHODS: This study categorized 104 subjects into 4 obesity phenotypes based on BMI and metabolic syndrome status: metabolically healthy obese (MHO), metabolically unhealthy obese (MUO), metabolically healthy non-obese (MHNO), and metabolically unhealthy non-obese (MUNO). Body composition was measured by dual-energy X-ray absorptiometry (DEXA) and metabolic profile was characterized by blood samples. All subjects completed the SF-36 item Short Form Health Survey Questionnaire. RESULTS: Comparing the four obesity phenotypes, significant results were reported for Bodily Pain between MHNO/MUNO (p = 0.034), for Vitality between MHO/MUO (p = 0.024), and for Mental Component Score between MHO/MUO (p = 0.026) and MUO/MUNO (p = 0.003). A more thorough inside-groups analysis yielded a positive and moderate to high correlation between FM parameters and QoL scales in MHO and MHNO, while a negative and weak to moderate correlation was observed in MUO and MUNO. CONCLUSION: This study reported an inverse U-shaped relationship between FM and QoL in obesity phenotypes, suggesting that metabolic status is a key factor involved in modulating QoL and therefore challenging the idea of obesity as a main driver of low QoL. We recommend the inclusion of FM percentage in the definition of obesity phenotypes in future research, to better evaluate QoL of obesity phenotypes.

6.
Polymers (Basel) ; 12(7)2020 Jun 30.
Artigo em Inglês | MEDLINE | ID: mdl-32630040

RESUMO

The dynamic evolution of materials with medical applications, particularly for drug delivery and wound dressing applications, gives impetus to design new proposed materials, among which, hydrogels represent a promising, powerful tool. In this context, multifunctional hydrogels have been obtained from chemically modified chitosan and acrylic polymers as cross-linkers, followed by subsequent conjugation with arginine. The hydrogels were finely tuned considering the variation of the synthetic monomer and the preparation conditions. The advantage of using both natural and synthetic polymers allowed porous networks with superabsorbent behavior, associated with a non-Fickian swelling mechanism. The in vitro release profiles for ibuprofen and the corresponding kinetics were studied, and the results revealed a swelling-controlled release. The biodegradability studies in the presence of lysozyme, along with the hemostatic evaluation and the induced fibroblast and stem cell proliferation, have shown that the prepared hydrogels exhibit characteristics that make them suitable for local drug delivery and wound dressing.

7.
Medicine (Baltimore) ; 98(26): e16069, 2019 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-31261515

RESUMO

The aim of this study was to check whether the sFlt-1/PIGF ratio, established as the biomarker for preeclampsia, reduces the false positive rate of late fetal growth restriction (FGR) detection by ultrasound biometry.This was a prospective case-control study, conducted at one regional maternity hospital in Romania. Study participants included singleton pregnancy women for whom the estimated fetal weight (EFW) at 28 to 35 weeks was < 10 percentiles and as controls, pregnant women with EFW >10 percentiles. All pregnancies were dated in the first trimester by crown-rump-length. We also recorded maternal characteristics, pregnancy and neonatal outcomes.The primary outcome measures were the relation between the sFlt-1/PIGF ratio and incidence of FGR. Secondary outcome was establishing a threshold for statistical significance of the marker and influence of other conditions (e.g., pre-eclampsia) on the accuracy of the marker in FGR prediction.Included in the study were 37 pregnant women and 37 controls.When we used ultrasound (US) biometry and maternal risk factors to estimate EFW <10 percentiles, the sensitivity was 44.4% with a specificity of 89% for an FPR (false positive result) of 10%. When we combined the US biometry and maternal risk factors with sFlt1/PIGF ratio, for a cut off of 38, the sensitivity was 84.21%, and the specificity was 84.31% for an FPR of 10%. The cut off value (36) did not change if we considered all cases of SGA, including those with associated preeclampsia or if we considered only FGR cases without associated preeclampsia.When associated with maternal factors and US biometry, the sFlt1/PIGF ratio enhanced the sensitivity for detecting late FGR.


Assuntos
Retardo do Crescimento Fetal/diagnóstico , Fator de Crescimento Placentário/sangue , Ultrassonografia Pré-Natal , Receptor 1 de Fatores de Crescimento do Endotélio Vascular/sangue , Adulto , Biomarcadores/sangue , Biometria , Estudos de Casos e Controles , Reações Falso-Positivas , Feminino , Retardo do Crescimento Fetal/epidemiologia , Humanos , Incidência , Gravidez , Segundo Trimestre da Gravidez , Terceiro Trimestre da Gravidez , Estudos Prospectivos , Fatores de Risco , Sensibilidade e Especificidade , Adulto Jovem
8.
Pediatr Int ; 55(5): 589-93, 2013 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-23659666

RESUMO

BACKGROUND: The aim of this study was to compare two neuroprotective strategies to supportive care in the treatment of perinatal asphyxia. METHODS: A total of 67 term newborns with perinatal asphyxia were included and randomized into three groups: one group received supportive treatment; another group received a single dose of 40 mg/kg phenobarbital; and the third received three daily doses of 1000 IU/kg erythropoietin. The following parameters were analyzed: gestational age, birthweight, Apgar scores, cord blood pH, total serum antioxidant status (TAS), superoxide dismutase (SOD), glutathione peroxidase (GPx) and malondialdehyde (MDA). The newborns were included in the follow-up program and examined up to 18 months of age. RESULTS: TAS was higher in the erythropoietin group than in the other groups. SOD and GPx were lower for infants treated with phenobarbital or erythropoietin compared to control infants. MDA was lower in the erythropoietin group compared to the other groups, although the difference was not statistically significant (P > 0.05). The mortality rate was lower in the phenobarbital and erythropoietin groups (both 4.6%) than in the control group (17.4%). Long-term neurologic follow up showed a high incidence of sequelae in the control group compared to the phenobarbital and erythropoietin groups. Follow-up results were better in the phenobarbital group than in the erythropoietin group for motor and cognitive function at 3 and 6 months and worse for expressive language. At 18 months, however, the differences between these two groups were not significant. CONCLUSION: High-dose phenobarbital or erythropoietin along with supportive treatment has a positive influence on the outcome of newborns with perinatal asphyxia. Phenobarbital has the advantage of low cost and simplicity.


Assuntos
Asfixia Neonatal/tratamento farmacológico , Eritropoetina/administração & dosagem , Fenobarbital/administração & dosagem , Asfixia Neonatal/metabolismo , Peso ao Nascer , Relação Dose-Resposta a Droga , Epoetina alfa , Feminino , Seguimentos , Idade Gestacional , Hematínicos/administração & dosagem , Humanos , Hipnóticos e Sedativos/administração & dosagem , Recém-Nascido , Injeções Intravenosas , Injeções Subcutâneas , Masculino , Estresse Oxidativo/efeitos dos fármacos , Estudos Prospectivos , Proteínas Recombinantes/administração & dosagem , Resultado do Tratamento
9.
Rev Med Chir Soc Med Nat Iasi ; 117(1): 59-64, 2013.
Artigo em Inglês | MEDLINE | ID: mdl-24505893

RESUMO

UNLABELLED: Measurement of arterial stiffness is an accurate method of assessment of endothelial dysfunction, together with other noninvasive methods, in the diagnosis of atherosclerotic burden in patients with MetS. MATERIAL AND METHODS: The study included 63 patients: MetS group (18 men, 20 women, mean age 58.86 +/- 8.86 years) and the control group (14 men, 11 women, mean age 59.68 +/- 10.0 years). They underwent the following examinations: assessment of arterial stiffness--pulse wave velocity (PWVao), augmentation index of brachial artery (Aixbr) and aorta (Aixao), central systolic blood pressure (SBPao); carotid ultrasound for detection of plaques and measurement of intima-media thickness (IMT); echocardiography--left ventricular hypertrophy (LVH); ankle-brachial index (ABI); biochemical parameters: C-reactive protein (CRP), fibrinogen (Fb), cholesterol (Col), HDLcol, LDLcol and triglycerides. RESULTS: MetS patients had higher PWVao (10.06 +/- 2.12 m/s vs 8.29 +/- 1.33 m/s, p = 0.0001) and SBPao (135.06 +/- 19.80 mmHg vs. 121.76 +/- 18.62 mmHg, p = 0.009). Carotid IMT was higher in MetS group (0.92 +/- 0.11 vs. 0.83 +/- 0.10 mm, p = 0.003). Almost all MetS patients were hypertensive (94.7% vs. 52%, p = 0.01); LVH was present in 57.9% of MetS patients and 20% of the controls (p = 0.05). The MetS group presented higher Col (208.76 +/- 38.41 vs. 176.20 +/- 30.08 mg/dl, p = 0.0003) and CRP levels (0.872 +/- 0.852 mg/dl vs. 0.476 +/- 0.392 mg/dl, p = 0.01). CONCLUSIONS: In MetS patients the most reliable marker of arterial stiffness was PWVao, followed by SBPao. Higher values of carotid IMT are also parameters of high atherosclerotic risk. CRP and Col can be considered biomarkers of high risk in MetS.


Assuntos
Aterosclerose/diagnóstico , Artérias Carótidas , Espessura Intima-Media Carotídea , Pacientes Internados , Síndrome Metabólica/diagnóstico , Rigidez Vascular , Idoso , Índice Tornozelo-Braço , Aterosclerose/sangue , Aterosclerose/diagnóstico por imagem , Aterosclerose/etiologia , Biomarcadores/sangue , Índice de Massa Corporal , Proteína C-Reativa/metabolismo , Artérias Carótidas/diagnóstico por imagem , Artérias Carótidas/fisiopatologia , Estudos de Casos e Controles , HDL-Colesterol/sangue , LDL-Colesterol/sangue , Feminino , Fibrinogênio/metabolismo , Humanos , Hipertrofia Ventricular Esquerda , Masculino , Síndrome Metabólica/sangue , Síndrome Metabólica/complicações , Síndrome Metabólica/diagnóstico por imagem , Pessoa de Meia-Idade , Análise de Onda de Pulso , Medição de Risco , Triglicerídeos/sangue
10.
Rev Med Chir Soc Med Nat Iasi ; 114(4): 1246-53, 2010.
Artigo em Romano | MEDLINE | ID: mdl-21500486

RESUMO

UNLABELLED: The polyurethanes are very used materials for medical devices. Their segmented block-copolymeric structures endows them a wide range of versatility in terms of tailoring their physical properties, and tissue compatibility. MATERIAL AND METHOD: A new polyether-urethane based material (PU-PTHF/MDI/HPC) was studied on bio-integration capacity in cardiovascular applications. In this respect, surface and bulk structure analysis, albumin and fibrinogen adsorption (in noncompetitive and competitive condition) and thrombogenicity have been evaluated. It was found that PU-PTHF/MDI/HPC surfaces had different roughness and bulk interconnected, well developed porous structure. As protein adsorption properties, preferential adsorption for albumin of material was observed. RESULTS: Thus, 0.280 +/- 0.04 mg/cm2 and 0.260+0.01 mg/ cm2 of albumin adsorb on PU-PTHF/MDI/HPC in noncompetitive and competitive conditions, while fibrinogen adsorption was 0.044 +/- 0.02 mg/cm2 and 0.040 +/- 0.01 mg/cm2. The clot amount test experiment have shown that clot weight of control blood was 24.8 +/- 2.03 mg, the clot weight formed by polyurethane was 28.4 +/- 2.93 mg and the clot weight formed by thrombogenic positive control (collagen membrane) was 42.5 +/- 3.50 mg. CONCLUSIONS: From our study we could conclude: 1--PU-PTHF/MDI/HPC polyurethane membranes have two-interface structure that could be advantageous for cavitary or tubular medical devices; 2--the protein adsorption results and clot formation test revealed a thromboresistent behavior of PU-PTHF/MDI/HPC, appropriate for cardiovascular applications.


Assuntos
Próteses Valvulares Cardíacas , Teste de Materiais , Polimerização , Poliuretanos/química , Propriedades de Superfície , Adsorção , Algoritmos , Materiais Biocompatíveis/química , Equipamentos e Provisões , Humanos , Teste de Materiais/métodos , Membranas Artificiais , Poliuretanos/síntese química , Estresse Mecânico
11.
J Mater Sci Mater Med ; 20(3): 775-83, 2009 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-19020959

RESUMO

Surface tension parameters and surface morphology of biopolyurethanes based on cellulose derivatives thin films, before and after HF cold plasma treatment has been investigated. Calculations are based on the geometric mean approach of Owens and Wendt, Rabel and Kälble, on the Lifshitz-van der Waals acid/base approach of van Oss and co-workers and on the theoretical methods involving quantitative structure-property relationship. For all the investigated samples the polar component contributes significantly to the total surface tensions, as due to the large electron donor interactions. HF cold plasma treatment modifies the surface energy of biopolyurethanes by changing their surface polarity and hydrophilicity. The hydrophilic/hydrophobic balance was studied by means of the free energy of hydration between the biomaterial film and water. The protein adsorption tests of fibrinogen were effected to evaluate the applicability of these biopolyurethanes as biomedical thromboresistant devices.


Assuntos
Materiais Biocompatíveis/química , Biopolímeros/química , Celulose/química , Poliuretanos/química , Adsorção , Fibrinogênio/metabolismo , Humanos , Técnicas In Vitro , Teste de Materiais , Microscopia Eletrônica de Varredura , Peso Molecular , Propriedades de Superfície , Tensão Superficial , Termodinâmica , Trombose/prevenção & controle , Água/química
12.
Rev Med Chir Soc Med Nat Iasi ; 108(4): 924-7, 2004.
Artigo em Romano | MEDLINE | ID: mdl-16004244

RESUMO

The red cells exhibit a proper magnetism due to hemoglobin. In a prototype of high gradient magnetic separator equipped with an ordered ferromagnetic matrix, a set of experiments with blood to determine the influence of the process parameters on the efficiency of the erythrocytes capture was realized. Dependent on the values of the magnetic induction (1.76-2.01 T), average blood flow velocity through the matrix (0.55-1.1 mm/s), and stationary time in the matrix, different values of concentration for the red cells in the matrix were obtained. For tests realized with integral blood, the concentration was approximately 20%, while for tests with diluted blood the concentration fluctuated from 14.51% to 29.90%. A blood recirculation through the matrix led to a concentration of 37.86%. The magnetic separation method permits an acceptable red cells concentration, without apparent destructive effects on the cells.


Assuntos
Separação Celular/métodos , Fenômenos Eletromagnéticos , Eritrócitos , Hemoglobinas/análise , Humanos
13.
Rev Med Chir Soc Med Nat Iasi ; 106(4): 796-800, 2002.
Artigo em Romano | MEDLINE | ID: mdl-14974232

RESUMO

UNLABELLED: The aim of the study was to determine the acute toxicity and the antimicrobial actions of an original magnetic carrier, type ferrofluid. The hydrophilic ferrofluid was prepared by covering the Fe3O4 nannoparticles with ammoniumoleate. The absolute amount of iron was of 40 mg/ml ferrofluid. METHODS: Acute toxicity was evaluated on five groups of Swiss male mice, after a single intraperitoneal administration of 1, 0.75, 0.5, 0.25 and 0.125 ml dose of pure ferrofluid/100 g body weight (b.w.), using step-level toxicity method. The study groups of mice were follow-up for 10 days. We did not use the same volume of solution for all the study groups because we were concerned about not to modify the behavior of the ferrofluid (but for each group we used the same volume of solution). The tasks of this part of the study were: 1) the record of the mice death in the first 10 days after intraperitoneal administration of ferrofluid; 2) the behavior of the animal subjects; 3) the morphopathologic examination of kidney, lung, heart, liver and peritoneum samples from the death mice and from the after ten days survivors which were sacrificed. We also investigated the possible antibacterial actions of the ferrofluid on E. coli spp., Klebsiella spp., Staphylococcus aureus Streptococcus group D., in the second part of the study, using standard lab kit. The validation of the results was performed using controls for E. colli and Staphylococcus aureus. RESULTS: The death of the mice was registered between 24 and finished after 96 hours. The maximum tolerated dose (MTD), was of 0.25 ml (10 mg iron/100 g b.w.) and the lethal dose hundred percent (LD100) was of 0.75 ml/(30 mg iron/100 b.w.). In our study we did not determined any kind of antibacterial action of the ferrofluid. CONCLUSIONS: 1) LD100, in our study, was of 30 mg iron/100 g b.w., and DMT of 10 mg iron/100 g b.w. 2) The death of the mice may be due to toxic aggression of ammonium ions released, in vivo, from the ammoniumoleate coverage of magnetite nannoparticles. 3) There were no in vitro antibacterial actions for this ferrofluid.


Assuntos
Meios de Contraste/farmacologia , Ferro/farmacologia , Óxidos/farmacologia , Animais , Meios de Contraste/toxicidade , Avaliação Pré-Clínica de Medicamentos , Óxido Ferroso-Férrico , Ferro/toxicidade , Magnetismo , Masculino , Camundongos , Testes de Sensibilidade Microbiana , Modelos Animais , Óxidos/toxicidade
SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA