RESUMO
We have developed an InAs/InP quantum dot (QD) C-band coherent comb laser (CCL) module with actively stabilized absolute wavelength and power, and channel spacing of 34.462 GHz with ± 100 ppm accuracy. The total output power is up to 46 mW. The integrated average relative intensity noise (RIN) values of the lasing spectrum and a filtered single channel at 1540.19 nm were -165.6 dB/Hz and -130.3 dB/Hz respectively in the frequency range from 10 MHz to 10 GHz. The optical linewidth of the 45 filtered individual channels between 1531.77 nm to 1543.77 nm ranged from 850 kHz to 2.16 MHz. We have also analyzed the noise behaviors of each individual channel.
RESUMO
Lead (Pb), a heavy metal, has become a crucial pollutant in soil and water, causing not only permanent and irreversible health problems, but also substantial reduction in crop yields. In this study, we conducted proteome analysis of the roots of the non-hyperaccumulator inbred maize line 9782 at four developmental stages (0, 12, 24, and 48 h) under Pb pollution using isobaric tags for relative and absolute quantification technology. A total of 252, 72 and 116 proteins were differentially expressed between M12 (after 12-h Pb treatment) and CK (water-mocked treatment), M24 (after 24-h Pb treatment) and CK, and M48 (after 48-h Pb treatment) and CK, respectively. In addition, 14 differentially expressed proteins were common within each comparison group. Moreover, Cluster of Orthologous Groups enrichment analysis revealed predominance of the proteins involved in posttranslational modification, protein turnover, and chaperones. Additionally, the changes in protein profiles showed a lower concordance with corresponding alterations in transcript levels, indicating important roles for transcriptional and posttranscriptional regulation in the response of maize roots to Pb pollution. Furthermore, enriched functional categories between the successive comparisons showed that the proteins in functional categories of stress, redox, signaling, and transport were highly up-regulated, while those in the functional categories of nucleotide metabolism, amino acid metabolism, RNA, and protein metabolism were down-regulated. This information will help in furthering our understanding of the detailed mechanisms of plant responses to heavy metal stress by combining protein and mRNA profiles.
Assuntos
Chumbo/toxicidade , Proteínas de Plantas/genética , Raízes de Plantas/efeitos dos fármacos , Proteoma/genética , Poluentes do Solo/toxicidade , Transcriptoma , Zea mays/efeitos dos fármacos , Aminoácidos/metabolismo , Proteínas de Transporte/genética , Proteínas de Transporte/metabolismo , Regulação da Expressão Gênica no Desenvolvimento , Regulação da Expressão Gênica de Plantas , Chaperonas Moleculares/genética , Chaperonas Moleculares/metabolismo , Anotação de Sequência Molecular , Oxirredução , Proteínas de Plantas/metabolismo , Raízes de Plantas/genética , Raízes de Plantas/crescimento & desenvolvimento , Raízes de Plantas/metabolismo , Proteoma/metabolismo , RNA de Plantas/genética , RNA de Plantas/metabolismo , Transdução de Sinais , Estresse Fisiológico , Zea mays/genética , Zea mays/crescimento & desenvolvimento , Zea mays/metabolismoRESUMO
Genetic map containing 103 microsatellite loci obtained on 200 F2 plants derived from the cross R15 x 478 was used for quantitative trait loci (QTL) mapping in maize. QTL were characterized in a population of 200 F2:4 lines, derived from selfing the F2 plants, and were evaluated with two replications in two environments. QTL determinations were made from the mean of these two environments. Plant height (PH) and ear height (EH) were measured. Using composite interval mapping (CIM) method, a total of 14 distinct QTLs were identified: nine for PH and five for EH. Additive, partial dominance, dominance, and overdominance actions existed among all detected QTL affecting plant height and ear height. The QTL explained 78.27% of the phenotypic variance of PH and 41.50% of EH. The 14 QTLs displayed mostly dominance or partial dominance gene action and mapped to chromosomes 2, 3, 4, 8 and 9.
Assuntos
Cromossomos de Plantas/genética , Repetições de Microssatélites/genética , Locos de Características Quantitativas , Zea mays/anatomia & histologia , Zea mays/genética , Mapeamento Cromossômico , Genes Dominantes , Genes de Plantas , FenótipoRESUMO
The effects of cadmium and phenobarbital (PB) administration on the activities of microsomal mixed-function oxidase (MFO) in rat kidney cortex and liver were studied. Five indices, including cytochrome P-450 contents, aniline hydroxylase, NADPH-cytochrome c reductase, aminopyrine N-demethylase and 7-ethoxycoumarin O-deethylase activities, were assayed. By comparison, it was found that PB could induce MFO activities in kidney cortex microsomes and liver microsomes, both hepatic and renal microsomal MFO were decreased by the ip injection of CdCl2, and Cd2+ could markedly decrease the phenobarbital induction to hepatic and renal microsomal MFO activities with the exception of NADPH-cytochrome c reductase in kidney cortex microsomes.
