A case series on safety and tolerability of human umbilical cord-derived mesenchymal stem cells on patients in Malaysia.

Umbilical cord-derived mesenchymal stem cells for regenerative therapy are a promising treatment option for chronic illnesses. Umbilical cord-derived mesenchymal stem cells offer several advantages over other sources, which makes them an attractive option in tissue repair and regeneration. This clinical study describes a 1-year follow-up on the safety and tolerance of umbilical cord-derived mesenchymal stem cell therapy on nine patients in Malaysia. Patients were assessed for adverse effects, and liver function tests were carried out on both pre- and post-treatments. Umbilical cord-derived mesenchymal stem cells' effectiveness and safety were assessed by follow-up evaluations. All nine patients responded positively towards umbilical cord-derived mesenchymal stem cell therapy, without any adverse effects. After umbilical cord-derived mesenchymal stem cell therapy, a significant improvement was observed in liver functioning test outcomes, as haematological parameters and tumour markers were stable. The present study concludes that umbilical cord-derived mesenchymal stem cell therapy is well tolerated by Malaysian patients; however, further clinical screening must be done over a large number of patients population.

[The effect of serum concentration on the growth, proliferation and collagen secretion in mouse L929 fibroblasts].

AIM: To observe the effect of serum concentration on the growth, proliferation and secretion of collagen I, collagen III and hydroxyproline in mouse fibroblasts L929, to optimize serum concentration for cell growth, proliferation and collagen secretion. METHODS: L929 cells were divided into 6 groups cultured in the present of 0 mL/L, 200 mL/L, 400 mL/L, 600 mL/L, 800 mL/L and 1 000 mL/L serum respectively. Acridine Orange and HE staining were performed on 2 d, 4 d, 6 d and 8d and sulforhodamine B sulfonyl chloride was used to determine cell proliferation at the same time; Meanwhile, the supernatants of each group ware collected and enzyme immunoassay was performed to detect the protein levels of collagen I, III and hydroxyproline. RESULTS: (1) The cell proliferation was inhibited when serum concentration was very high (100%) or very low (0%). The proliferation rate in the presence of 20% serum was the highest. When serum concentration was higher than 20%( 40%-100%), the cell proliferation decreased. (2) Apoptotic cells were observed in the absence of serum. Vacuolization appeared in the presence of 80% serum, and was enhanced in the case of 100% serum. Cells were morphologically normal when serum concentrations were between 20%-60%. (3)The level of collagen I was low in the case of serum concentrations of 0%, 80% and 100%, with the highest expression in 40% serum. Collagen III in all groups was increased on day 2, followed by a transient decreased and an final increased on day 6-8. The level of hydroxyproline was not changed in all groups. CONCLUSION: The serum concentrations between 20%-40% is suitable for L929cells growth, proliferation and secret collagen. The extremes serum concentration of too high or too low leads to inhibition of cell proliferation, apoptosis, vacuolization, and impaired secretion of collagens. This study indicates the requirement of optimum serum concentration for self serum injection therapy.

[Neuropeptide Y promotes the proliferation and differentiation of 3T3-L1 preadipocytes].

AIM: To observe the effect of neuropeptide Y on the proliferation and differentiation of 3T3L1 preadipocytes and detect the mechanics. METHODS: 3T3-L1 preadipocytes were induced to differentiation by the cocktail medium containing 3-isobutyl-1-methylxanthine (IBMX), dexamethasone and insulin. After 2 days, the cells were divided into 3 groups: the control group(without inducer), the routine group(insulin used as inducer) and the experiment group(10(-8), 10(-9), 10(-10) mol/L NPY used as inducer). At day 7 and 12, cell differentiation was observed by phase contrast microscope. At day 12, Oil red O staining was performed. Proliferation capacities of 3T3L1 cells were assessed by MTT. Western blot was used to know the protein level of peroxisome proliferator-activated receptor gamma (PPAR-γ) and The CCAAT/enhancer-binding protein-α(C/EBP-α). RESULTS: NPY 10(-8) mol/L promotes the differentiation of 3T3L1 preadipocytes, 10(-9) mol/L, 10(-8) mol/L NPY increased 3T3-L1 cells proliferation. The protein levels of PPAR-γ and C/EBP-α were up regulated by 10(-8) mol/L NPY. CONCLUSION: Neuropeptide Y promotes the differentiation and Proliferation of 3T3-L1 cells, this effect may be related to up-regulation of PPAR-γ and C/EBP-α.