GC-MS and LC-MS: an integrated approach towards the phytochemical evaluation of methanolic extract of Pigeon Pea [Cajanus cajan (L.) Millsp] leaves.

Pigeon Pea is a well-received traditional nutritious and medicinal plant in India since ancient times. The plant is identified for its impressive pharmacological activities like antimicrobial, anti-inflammatory, antitubercular, antioxidant, neuroprotective, antihypertensive, antihyperglycemic and anticancer properties. The metabolites from Pigeon Pea leaves methanolic extract were identified using hyphenated instruments namely Gas Chromatography-Mass Spectroscopy (GC-MS) and Liquid Chromatography-Mass Spectroscopic (LC-MS) method. GC and LC chromatogram showed proper separation and presence of several bioactive compounds such as flavonoids, terpenoids, essential oils, alkaloids, and coumarins. LC-MS investigation reported the presence of Selidin (coumarin) and Tamarixetin (monomethoxyflavone) in the Pigeon Pea Leaves. The present investigations have demonstrated that methanolic extract of Pigeon Pea leaves exhibited impressive antibacterial activity against E. coli and antifungal activity against C. albicans with MIC values 50 µg/ml 250 µg/ml respectively. The extracts studied here have demonstrated to be a new remedial source used against diseases caused by these microorganisms.

Phytochemical analysis by GC-MS, LC-MS complementary approaches and antimicrobial activity investigation of Vigna unguiculata (L.) Walp. leaves.

Consumption of legumes has long been linked to their nutritional and medicinal benefits. Vigna unguiculata (L.) Walp. (Cowpea) is a legume plant in the Fabaceae family and is a rich source of nutrients also is known for its beneficial effects for diseases treatment. In terms of phytochemicals analysis and bioactivities evaluations the major research has focused on the Cowpea seeds, whereas leaves and pods are remained understudied. Herein we have highlighted leaves methanolic extract phytochemicals identification, antimicrobial, and antioxidant activity assessment. Cowpea leaves methanolic extract Liquid Chromatography-Mass Spectrometry (LC-MS) analysis first time revealed the presence of α-hederin, which is a putative novel SARS-COV-2 inhibitor and Zearlenone mycotoxin. Leaves methanolic extract exhibited strong activity against Streptococcus pyogens and Candida albicans. The Cowpea leaves extract is a potent DPPH inhibitor with an IC50 of 62.04 ± 0.08 µg/mL. The bioactive compounds identification in this work supports the plant's nutritional and medicinal uses.