Optimized robotic setup for automated active thermography using advanced path planning and visibility study.

In-line inspection of advanced components remains a challenging task in industry. The authors will describe an automated methodology that uses numerical simulations to automatically determine the best set of experimental parameters to inspect the structure on defects using active thermography. The inspection is performed using a robotic arm and advanced path-planning tools to determine the optimal positions of the measurement points and excitation points. During the path planning, the directional emissivity is considered for the complex surface, and a minimization of the amount of measurement points is performed. The numerical simulation optimization used a genetic algorithm and spline regression model to optimize the heat power, robot speed, camera frame rate, and excitation timing to fulfill the automatic inspection.

Comparison of the accuracy of digital stereophotogrammetry and projection moiré profilometry for three-dimensional imaging of the face.

The aim of this study was to compare the three-dimensional (3D) imaging accuracy between a digital stereophotogrammetry device and a projection moiré profilometry setup using anatomical models in conjunction with surface matching software. Twenty-two 3D surface models of the middle third of the face derived from computed tomography (CT) scans were used to fabricate photopolymer models by rapid prototyping. These were digitized using digital stereophotogrammetry and projection moiré profilometry. The 3D surface models acquired were compared for shape differences with the original CT models using surface matching software. Global registration between each pair of corresponding models was carried out using an iterative closest point algorithm. The mean surface deviations following registration were used to calculate Bland-Altman limits of agreement between the two methods. The distributions of measured surface differences were used to calculate L-moments. Paired t-tests were carried out for hypothesis testing. Correlation between difference and mean was -0.3, and 95% limits of agreement were -0.084mm and 0.064mm. No statistically significant differences in mean measurement error (L1 moments) were observed (P=0.1882). The experimental moiré profilometry setup employed produced 3D models of facial anatomy of comparable accuracy to a widely used commercialized digital stereophotogrammetry device.

Nonlinearity in eardrum vibration as a function of frequency and sound pressure.

It is generally accepted that the middle ear acts mainly as a linear system for sound pressures up to 130 dB SPL in the auditory frequency range. However, at quasi-static pressure loads a strong nonlinear response has been demonstrated. Consequently, small nonlinear distortions may also be present in the middle ear response in the auditory frequency range. A new measurement method was developed to quickly determine vibration response, nonlinear distortions and noise level of acoustically driven biomechanical systems. Specially designed multisines are used for the excitation of the test system. The method is applied on a gerbil eardrum for sound pressures ranging from 90 to 120 dB SPL and for frequencies ranging from 125 Hz to 16 kHz. The experiments show that nonlinear distortions rise above noise level at a sound pressure of 96 dB SPL, and they grow as sound pressure increases. Post-mortem changes in the middle ear influence the nonlinear distortions rapidly until a stabilization occurs after approximately 3h.

Atmospheric pressure fluctuations in the far infrasound range and emergency transport events coded as circulatory system diseases.

This study examines whether a relation exists between rapid atmospheric pressure fluctuations, attributed to the far infrasound frequency range (APF), and a number of emergency transport events coded as circulatory system diseases (EEC). Over an entire year, the average integral amplitudes of APF in the range of periods from 3 s to 120 s over each hour (HA) were measured. Daily dynamics of HA averaged over the year revealed a wave shape with smooth increase from night to day followed by decrease from day to night. The total daily number of EEC within the city of Kiev, Ukraine, was related to the daily mean of HA (DHA) and to the ratio of HA averaged over the day time to HA averaged over the night time (Rdn), and was checked for confounding effects of classical meteorological variables through non-parametric regression algorithms. The number of EEC were significantly higher on days with high DHA (3.72-11.07 Pa, n = 87) compared to the low DHA (0.7-3.62 Pa, n = 260, p = 0.01), as well at days with low Rdn (0.21-1.64, n = 229) compared to the high Rdn (1.65-7.2, n = 118, p = 0.03). A difference between DHA and Rdn effects on the emergency events related to different categories of circulatory diseases points to a higher sensitivity of rheumatic and cerebro-vascular diseases to DHA, and ischaemic and hypertensive diseases to Rdn. Results suggest that APF could be considered as a meteorotropic factor capable of influencing circulatory system diseases.

Morphology and function of Bast's valve: additional insight in its functioning using 3D-reconstruction.

The utriculo-endolymphatic valve was discovered by Bast in 1928. The function of Bast's valve is still unclear. By means of orthogonal-plane fluorescence optical sectioning (OPFOS) microscopy 3D-reconstructions of the valve and its surrounding region are depicted. The shape of the duct at the utricular side is that of a flattened funnel. In the direction of the endolymphatic duct and sac this funnel runs into a very narrow duct. The valve itself has a rigid 'arch-like' configuration. The opposing thin, one cell-layer thick, utricular membrane is highly compliant. We propose that opening and closure of the valve occurs through movement of the flexible base/utricular membrane away from and toward the relatively rigid valve lip.

Improved correction of axial geometrical distortion in index-mismatched fluorescent confocal microscopic images using high-aperture objective lenses.

Extracting quantitative data from microscopic volume images is straightforward when the refractive indices of the immersion medium and the mounting medium are equal. The readings of the position of the specimen stage can be directly used to measure depth and width. Imperfectly matched immersion and mounting media result in axial geometrical distortion. Linear correction of the axial distortion using the paraxial estimate of the axial scaling factor yields results that may differ as much as 4% from the actual values. From calculations based on a theoretical expression of the 3-D point-spread function in the focal region of a high-aperture microscope focussing into a mismatched mounting medium, we derived axial scaling factors that result in quantitative results accurate to better than 1%. From a non-linear correction procedure, an improved formula for the paraxial estimate of the axial scaling factor is derived.

Scala vestibuli pressure and three-dimensional stapes velocity measured in direct succession in gerbil.

It was shown that the mode of vibration of the stapes has a predominant piston component but rotations producing tilt of the footplate are also present. Tilt and piston components vary with frequency. Separately it was shown that the pressure gain between ear canal and scala vestibuli was a remarkably flat and smooth function of frequency. Is tilt functional contributing to the pressure in the scala vestibuli and helping in smoothing the pressure gain? In experiments on gerbil the pressure in the scala vestibuli directly behind the footplate was measured while recording simultaneously the pressure produced by the sound source in the ear canal. Successively the three-dimensional motion of the stapes was measured in the same animal. Combining the vibration measurements with an anatomical shape measurement from a micro-CT (CT: computed tomography) scan the piston-like motion and the tilt of the footplate was calculated and correlated to the corresponding scala vestibuli pressure curves. No evidence was found for the hypothesis that dips in the piston velocity are filled by peaks in tilt in a systematic way to produce a smooth middle ear pressure gain function. The present data allowed calculations of the individual cochlear input impedances.

The mechanical reaction of the pars flaccida of the eardrum to rapid air pressure oscillations modeling different levels of atmospheric disturbances.

Atmospheric pressure fluctuations (APF) might induce mechanical effects in the pars flaccida (PF) of the eardrum. To clarify these effects, different kinds of pressure oscillations (PO), chosen within the range of naturally occurring APF, were applied to the middle ears (ME) of gerbils. The linear displacement of the PF during a PO in the ME was measured by laser interferometry. The compliance of the PF to PO was calculated as the ratio of the amplitude of a PF oscillation to the amplitude of a PO. The displacement of the PF traced the PO in the entire range of frequencies (from 10mHz to 200mHz) and amplitudes (from 10Pa to 110Pa) applied to the ME. Moreover, the PF is found to be displaced by pressure pulses of a few pascals only using a PO with a complex shape. The differences found in the compliance of the PF due to PO with low (less than 20Pa) and high (more than 90Pa) amplitude point out that the mechanism of pressure regulation in the ME through the mechanical reaction of the PF in gerbil ears is better adapted to ordinary levels of natural APF than to extraordinary levels. The implications of these findings for the physiology of the human ME with respect to adaptation to natural APF are discussed.

The effects of slight pressure oscillations in the far infrasound frequency range on the pars flaccida in gerbil and rabbit ears.

This study was designed to clarify whether the pars flaccida (PF) as a flexible part of the tympanic membrane is capable of reacting to pressure oscillations (PO) with amplitudes and frequencies typical for natural atmospheric pressure fluctuations in the far infrasound frequency range (APF). If so, the PF mechanical reactions to APF might be involved in the overall physiologic regulation processes, which make organisms susceptible to APF. The displacements of the PF in response to PO were measured in vitro in ears of gerbils and rabbits by means of laser Doppler vibrometry. The index of the PF reactivity (R(a)) was determined as the ratio of the amplitude of the PF oscillations (PFO) to the amplitude of the PO. All kinds of PO applied caused PFO. The amplitude of the PFO increased when the amplitude of the PO was increased. In gerbils, a decrease in R(a) with the increase in amplitude of the PO was observed. In the range of PO lowest amplitudes (4-20 Pa) R(a) proved to be 1.4 times higher than in the range of highest amplitudes (90-105 Pa). Considering that the natural APF are usually within the range of +/-20 Pa, this fact points to an important contribution of the PF to the pressure dynamics in the middle ear (ME) of gerbils. In rabbit ears, R(a) was lower and recovery from plastic deformation was slower than in gerbils. Our findings are in line with the suggestion that the PF might play an important role in respect of adaptation to natural APF.

Thickness distribution of fresh eardrums of cat obtained with confocal microscopy.

The aim of this study was to measure the spatial thickness distribution of the cat tympanic membrane (TM), a very thin, virtually transparent and delicate biological membrane. Axial fluorescence images taken perpendicular through isolated TM were recorded for five different cats using confocal laser scanning microscopy. Thickness was measured on the cross-section of the membranes in the axial images. A correction for focal shift due to refractive-index mismatch was applied. Similar thickness distributions were obtained in all measured samples (n = 9). The pars tensa had a rather constant thickness in the central region between the annulus and manubrium. The thickness increased steeply toward the peripheral rim. Thickness was smallest in the inferior region, with values ranging between 5.5 and 9 microm in the central part and up to 50 microm near the annulus. More superiorly, thickness was slightly higher, up to 20 microm, between the annulus and manubrium. The anterior part was thicker than the posterior side. These findings are strongly different from a current value in the literature. Our data allow a more precise representation of the eardrum in mathematical models, which are a prerequisite for a better understanding of middle-ear mechanics. The optical sectioning technique of the confocal microscope did not result in any preparation artifacts and was therefore also used to quantify shrinkage due to preparation of histological sections of TMs.

A procedure to determine the correct thickness of an object with confocal microscopy in case of refractive index mismatch.

A difference in refractive index (n) between immersion medium and specimen results in increasing loss of intensity and resolution with increasing focal depth and in an incorrect axial scaling in images of a confocal microscope. Axial thickness measurements of an object on such images are therefore not exact. The present paper describes a simple procedure to determine the correct axial thickness of an object with confocal fluorescence microscopy. We study this procedure for a specimen that has a higher refractive index than the immersion medium and with a thickness up to 100 microm. The measuring method was experimentally tested by comparing the thickness of polymer layers measured on axial images of a confocal microscope in case of a water-polymer mismatch to reference values obtained from an independent technique, i.e. scanning electron microscopy. The case when the specimen has a lower refractive index than the immersion medium is also shown by way of illustration. Measured thickness data of a water layer and an oil layer with the same actual thickness were obtained using an oil-immersion objective lens with confocal microscopy. Good agreement between theory and experiment was found in both cases, consolidating our method.

A simple method for checking the illumination profile in a laser scanning microscope and the dependence of resolution on this profile.

One of the conditions for a laser scanning microscope to reach its optimal performance is for it to operate at its full numerical aperture (NA). In most commonly used systems, the illumination intensity at the back focal plane of the objective lens is apodized. This paper presents a simple method using a photodiode for checking the actual illumination intensity profile. We show as an example the measured profiles of a laser beam when working with two high-NA immersion objectives in two different confocal systems, and also show that in theoretical studies of the point-spread function, the assumption of a flat compared with a truncated Gaussian beam profile gives rise to severe discrepancies. The measured profiles also serve as an indication of the necessity of a realignment of the optical system.

Three-dimensional modelling of the middle-ear ossicular chain using a commercial high-resolution X-ray CT scanner.

The quantitative measurement of the three-dimensional (3-D) anatomy of the ear is of great importance in the making of teaching models and the design of mathematical models of parts of the ear, and also for the interpretation and presentation of experimental results. This article describes how we used virtual sections from a commercial high-resolution X-ray computed tomography (CT) scanner to make realistic 3-D anatomical models for various applications in our middle-ear research. The important problem of registration of the 3-D model within the experimental reference frame is discussed. The commercial X-ray CT apparatus is also compared with X-ray CT using synchrotron radiation, with magnetic resonance microscopy, with fluorescence optical sectioning, and with physical (histological) serial sections.