RESUMO
BACKGROUND: Vanilla siamensis is listed in Appendix-II of Convention on International Trade in Endangered Species of Wild Fauna and Flora (CITES) as an endangered species in Thailand. OBJECTIVE: To develop an optimum cryopreservation protocol for V. siamensis. MATERIALS AND METHODS: Protocorms were precultured on solid ½ MS medium with 0.5 M sucrose for 0-7 d. For encapsulation-dehydration, encapsulated protocorms (beads) were dehydrated for 0-6 h. In the case of encapsulation-vitrification, the beads were loaded with a plant vitrification solution 2 (PVS2) at 0 ËC for 0-90 min. RESULTS: Protocorms precultured for 3 d gave the highest post-cryopreservation survival of 17%. Dehydration of the encapsulated protocorm beads for 4 h gave the highest survival of 33% and a regrowth of 25%. Protocorms subjected to the encapsulation-vitrification method did not survive at all. CONCLUSION: Protocorms precultured with 0.5 M sucrose for 3 d, encapsulated with 3% sodium alginate and dehydrated to a moisture content c. 14% before plunging into LN was a suitable method for the successful cryopreservation of V. siamensis protocorms.
RESUMO
We studied a population of hemodialysis (HD) patients in the Najran region of Saudi Arabia to assess the prevalence of hepatitis C virus (HCV) and to evaluate the possible risk factors associated with this infection. The records of 90 patients undergoing dialysis in two centers of this region were reviewed. Blood samples were screened for anti-HCV antibodies by enzyme linked immunosorbent assay (ELISA) and positive samples were tested for confirmation by recombinant immunoblot assay. Liver transaminases were measured to assess the activity of the virus. In this study, an overall HCV prevalence of 46.7% was found among the HD patients. Statistical analysis showed that the number of previous blood transfusions, duration of dialytic age and dialysis treatment in multi-centers were associated significantly with HCV seropositivity. Although the mean levels of liver enzymes were significantly higher in HCV-positive than in HCV-negative patients, enzyme levels were raised above normal in only 29% of the patients.
Assuntos
Cromossomos Humanos Par 4/genética , Ligação Genética/genética , Transtornos Heredodegenerativos do Sistema Nervoso/diagnóstico , Transtornos Heredodegenerativos do Sistema Nervoso/genética , Doença de Huntington , Repetições de Trinucleotídeos/genética , Criança , Consanguinidade , Diagnóstico Diferencial , Feminino , Marcadores Genéticos , Transtornos Heredodegenerativos do Sistema Nervoso/fisiopatologia , Humanos , Doença de Huntington/diagnóstico , Doença de Huntington/genética , Escore Lod , Arábia Saudita/etnologia , Síndrome , Gravação de VideoteipeRESUMO
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