Circulating free DNA and p53 antibodies in plasma of patients with ovarian epithelial cancers.

BACKGROUND: This study was conducted in order to evaluate the significance of circulating free DNA (CFDNA), blood plasma p53 antibodies (p53-Ab) and mutations of KRAS gene in the prognosis of ovarian epithelial cancers. PATIENTS AND METHODS: A total of 126 patients were included in this study. KRAS mutations and CFDNA were detected by means of the PCR-restriction fragment length polymorphism (PCR-RFLP) and enriched by the PCR-RFLP method. Enzyme-linked immunosorbent assay was used to analyze plasma p53-Ab. RESULTS: KRAS mutations were detected in 27 (21.4%) of examined tumors. The frequency of KRAS mutations was especially high in mucinous cancers (P < 0.001). CFDNA and p53-Ab were frequently detected in patients with serous cancers in high grade (P < 0.001). The overall survival rate was significantly lower for patients with serous tumors and CFDNA and p53-Ab-positive than negative tumors (P = 0.022 and P < 0.001, respectively). In mucinous ovarian cancer, a worse overall survival was correlated with the KRAS mutations (P = 0.03). CONCLUSIONS: The results of the present study suggested that a presence of KRAS mutations in mucinous ovarian cancer and CFDNA and p53-Ab in serous tumors was correlated with the highest risk of cancer progression.

Patterns of immunohistochemical staining for p53 expression in hyperplastic endometrium and adenocarcinoma.

The p53, a tumour suppressor gene, is the most commonly mutated gene human cancer. In this study, we performed immunohistochemical investigations of the expression of p53 protein in hyperplastic endometrium and adenocarcinoma. Positive immunostaining was detected in 7 (30%) cases of invasive adenocarcinoma, 2 (12%) cases of simple hyperplasia with atypia and 2 (14%) cases of complex hyperplasia with atypia. In simple and complex hyperplasia without atypia staining was seen in occasional cells. The results suggested that endometrial hyperplasia is not always accompanied by p53 protein accumulation, hence its expression is not an early exponent of the neoplastic process.

[Serum lipid levels in hypercholesterolemic post-menopausal women treated with transdermal sequential combined hormone replacement therapy only or in combination with simvastatin]. / Osoczowe stezenie lipidów u kobiet z hipercholesterolemia w trakcie przezskórnej sekwencyjnej zlozonej hormonalnej terapii zastepczej skojarzonej z podawaniem statyn.

The aim of the study was to evaluate the effects of transdermal sequential combined hormone replacement therapy alone or combined with simvastatin on the serum lipid profile in hypercholesterolaemic post-menopausal women.

[Expression of PCNA antigen in cellular proliferative activity of cervical carcinoma]. / Ekspresja antygenu PCNA w ocenie aktywnosci proliferacyjnej komorek raka szyjki macicy.

The proliferative activity of cervical carcinoma was examined using proliferating cell nuclear antigen (PCNA) staining. Labelling index (LI) have been counted in sections of routinely processed paraffin embedded tissue blocks. Significant variation in PCNA LI were observed between study groups (p < 0.0235). The results obtained indicate that the evaluation of proliferative activity of cells expressed by high PCNA LI is assessed with worse prognosis.

[Elective premature delivery: clinical studies]. / Elektywny poród przedwczesny (badania kliniczne).

Cases of placental dysfunction complicated additionally by premature delivery were assessed. Abnormalities of pregnancy leading to chronic deficit in oxygenation were analyzed. Clinical utility of diagnostic procedures was evaluated. Neonatal condition in cases of elective premature birth versus spontaneous preterm delivery was compared.