Assuntos
Anuros/embriologia , Embrião não Mamífero/citologia , Animais , Divisão Celular , MorfogêneseRESUMO
Influence of constant magnetic field (CMF-60 mT induction) on catecholamine content of the serous coats mast cell system was studied in 60 outbred albino rats by luminescent histochemical method. CMF exposition made twenty minutes daily during 70 days. Three days long one leaded to reduction of catecholamine content level in mast cell system. At the same time, CMF action from 6 to 10 days long caused increase of catecholamine level as compared to normal state in the structures studied. The data obtained were considered as a protective reaction of the organism, aimed to maintain the general homeostage balance.
Assuntos
Catecolaminas/análise , Magnetismo , Mastócitos/química , Animais , Feminino , Masculino , Mastócitos/citologia , Microscopia de Fluorescência , Ratos , Espectrometria de Fluorescência , Fatores de TempoRESUMO
It was shown that the permanent magnetic fields at 35-40 mT1 inhibit the activity of biogenic monoamines in developing amphibian embryos. The effect was quantitatively similar to that of PMF at 40 mT1 (3-hour exposure during 6 days). The most significant fluctuations in their activity were observed at the stages of blastoderm formation and gastrulation onset.
Assuntos
Anfíbios/embriologia , Monoaminas Biogênicas/metabolismo , Magnetismo , Anfíbios/metabolismo , Animais , Histocitoquímica , Rana ridibunda , Federação Russa , Coloração e Rotulagem/métodos , Fatores de TempoRESUMO
By means of luminescent histochemistry effect of a constant magnetic field with induction 60 mTl (exposition for 2, 6, 36 h and 7 days) has been studied in order to reveal contents of catecholamines in mesenteric mast cells and in the intestinal mesentery cells in 50 white Wistar rats. In 2-6 h specific luminescence of the mast cells increases, however, at prolongation of the exposure up to 30 h metabolism of catecholamines in the mast cells is inhibited noticeably++ (luminescence disappears). In 8 days amount of the mast cells and specific luminescence of catecholamines decrease. The essential shifts revealed in the system of the mast cells of the mammalian should be taken into consideration in the magnetic-therapeutic practice. Close spatial relations between the mast cells and the mesenteric adrenergic terminals have been elucidated, demonstrating their morphofunctional interconnection.
Assuntos
Líquido Ascítico/citologia , Catecolaminas/metabolismo , Campos Eletromagnéticos , Mastócitos/efeitos da radiação , Mesentério/citologia , Animais , Catecolaminas/efeitos da radiação , Contagem de Células/efeitos da radiação , Masculino , Mastócitos/citologia , Mastócitos/metabolismo , Camundongos , Camundongos Endogâmicos , Ratos , Ratos EndogâmicosRESUMO
A sharp intensification of catecholamines synthesis in mast cells regularly followed noradrenaline and reserpine injection. The method of autoradiography makes it possible to analyse quantitatively the intensification of catecholamines synthesis in the mast cells.
Assuntos
Di-Hidroxifenilalanina/metabolismo , Mastócitos/metabolismo , Norepinefrina/farmacologia , Reserpina/farmacologia , Animais , Autorradiografia , Catecolaminas/biossíntese , Feminino , Intestino Delgado/metabolismo , Masculino , Mastócitos/efeitos dos fármacos , Mesentério/metabolismo , Camundongos , Camundongos Endogâmicos , Miocárdio/metabolismo , Ratos , Ratos Endogâmicos , Pele/metabolismo , Timo/metabolismo , Língua/metabolismoRESUMO
Histochemical fluorescent technique employed in the in vitro experiment detected stimulating effect of PND on the levels of catecholamines in peritoneal mast cells.
Assuntos
Catecolaminas/metabolismo , Mastócitos/efeitos dos fármacos , Naftalenos/farmacologia , Animais , Células Cultivadas , Diaminas/farmacologia , Histocitoquímica , Medições Luminescentes , Mastócitos/metabolismo , Ratos , Ratos EndogâmicosRESUMO
Reproduction of mast cells has been investigated by means of H3-thymidine radioautography in 26 mature mice of CC-57 White line and in 40 new-born nonlinear albino rats under normal conditions and experimental cold effect. It is established that differentiated mast cells incorporate H3-thymidine and can enter the phase of DNA synthesis of a proliferative cycle.