RESUMO
In the present study, two new methods were developed for the quantitative determination of active components of Seretide(®), commercially available pharmaceutical preparation in the diskus form. One of these methods was based on derivative spectrophotometry and used a zero-crossing technique. The determinations of fluticasone propionate and salmeterol xinafoate were performed by first order derivatisation at 216.5 nm and second order derivatisation at 250 nm, respectively. The concentration ranges were 5.0-32.5 µg/mL for fluticasone propionate and 2-12 µg/mL for salmeterol xinafoate. The second method developed also included high performance liquid chromatography. In this method, a methanol-water mobile phase mixture (95:5, v/v) and a C18 chromasil column as a stationary phase were used. The wavelength of the diode array UV detector was 260 nm; the flow rate was 1 mL/min. The concentration ranges were 2-16 µg/mL for fluticasone propionate and 1-8 µg/mL for salmeterol xinafoate. The results for both methods from diskus are in the pharmacopea limits. For the statistical determination of these results, these two methods were compared with t-test for the means and with F-test for the standard deviations.
Assuntos
Agonistas de Receptores Adrenérgicos beta 2/análise , Albuterol/análogos & derivados , Androstadienos/análise , Antiasmáticos/análise , Broncodilatadores/análise , Cromatografia Líquida de Alta Pressão , Inaladores de Pó Seco , Espectrofotometria Ultravioleta , Administração por Inalação , Agonistas de Receptores Adrenérgicos beta 2/administração & dosagem , Albuterol/administração & dosagem , Albuterol/análise , Androstadienos/administração & dosagem , Antiasmáticos/administração & dosagem , Broncodilatadores/administração & dosagem , Calibragem , Cromatografia Líquida de Alta Pressão/normas , Combinação de Medicamentos , Fluticasona , Combinação Fluticasona-Salmeterol , Limite de Detecção , Modelos Lineares , Padrões de Referência , Reprodutibilidade dos Testes , Xinafoato de Salmeterol , Espectrofotometria Ultravioleta/normasRESUMO
Rofecoxib was assayed by UV spectrophotometry and HPLC, the concentration ranges were 2-30 microg x ml(-1) and 5-50 microg x ml(-1), respectively. The results, obtained by the two methods in pharmaceutical preparations were compared of each other. There were no significant differences between the mean values and the precisions.
Assuntos
Anti-Inflamatórios não Esteroides/análise , Inibidores de Ciclo-Oxigenase/análise , Lactonas/análise , Cromatografia Líquida de Alta Pressão , Ciclo-Oxigenase 2 , Inibidores de Ciclo-Oxigenase 2 , Etanol , Isoenzimas/metabolismo , Soluções Farmacêuticas , Prostaglandina-Endoperóxido Sintases/metabolismo , Padrões de Referência , Solventes , Espectrofotometria Ultravioleta , Sulfonas , Comprimidos/análiseRESUMO
1,4-Dihydro-3-(3-hydroxy-2-naphthyl)-4-substituted-5H-L2,4-triazolinc-5-thiones were synthesized. The structures of original eight compounds were confirmed by elemental analysis, 1H NMR and mass spectral methods. One of the compounds (3a) was tested in vitro for its anticancer activity against 52 human tumor cell lines.
Assuntos
Antineoplásicos/síntese química , Antineoplásicos/farmacologia , Tionas/síntese química , Tionas/farmacologia , Triazóis/síntese química , Triazóis/farmacologia , Antineoplásicos/química , Divisão Celular/efeitos dos fármacos , Relação Dose-Resposta a Droga , Ensaios de Seleção de Medicamentos Antitumorais , Humanos , Espectroscopia de Ressonância Magnética , Estrutura Molecular , Tionas/química , Células Tumorais CultivadasRESUMO
5-(4-Pyridinyl)-4-substituted-2.4-dihydro-3H-1,2,4-triazole-3-thiones and 5-(4-pyridinyl)-4-substituted-3-(benzoylmethyl)thio-4H-1,2,4-triazoles were synthesized. The structures of original nine compounds were confirmed by IR, 'H NMR, mass spectral methods and elemental analysis. The antibacterial, antifungal and antimycobacterial activities, together with those of known intermediate 1,4-disubstituted thiosemicarbazides, were reported.
Assuntos
Anti-Infecciosos/síntese química , Sulfetos/farmacologia , Triazóis/farmacologia , Antibacterianos , Anti-Infecciosos/química , Anti-Infecciosos/farmacologia , Bactérias/efeitos dos fármacos , Candida albicans/efeitos dos fármacos , Testes de Sensibilidade Microbiana , Estrutura Molecular , Análise Espectral , Relação Estrutura-Atividade , Sulfetos/síntese química , Sulfetos/química , Tionas/química , Triazóis/síntese química , Triazóis/químicaRESUMO
1-(3-Hydroxy-2-naphthoyl)-4-substituted thiosemicarbazides were obtained by the addition of 3-hydroxy-2-naphthoic acid hydrazide to various isothiocyanates. The structures of the synthesized compounds were confirmed using UV and 1H-NMR spectral methods together with elemental analysis.
Assuntos
Antibacterianos/farmacologia , Semicarbazidas/farmacologia , Antibacterianos/síntese química , Escherichia coli/efeitos dos fármacos , Klebsiella/efeitos dos fármacos , Testes de Sensibilidade Microbiana , Proteus/efeitos dos fármacos , Pseudomonas/efeitos dos fármacos , Semicarbazidas/síntese química , Staphylococcus aureus/efeitos dos fármacosRESUMO
In this study, some 1,4-disubstituted thiosemicarbazide, 1,2,4-triazole and 1,3,4-thiadiazole type novel compounds derived from 3-hydroxy-2-naphthoic acid hydrazide were synthesized to screen for their antimicrobial activity. The structures of these substances were elucidated using elemental analysis and UV, 1H NMR, and mass spectral methods. All of these compounds were tested in vitro for their antibacterial and antifungal activity.
Assuntos
Anti-Infecciosos/síntese química , Antifúngicos/síntese química , Semicarbazidas/síntese química , Anti-Infecciosos/química , Anti-Infecciosos/farmacologia , Antifúngicos/química , Antifúngicos/farmacologia , HIV/efeitos dos fármacos , Testes de Sensibilidade Microbiana , Estrutura Molecular , Neoplasias/tratamento farmacológico , Semicarbazidas/química , Semicarbazidas/farmacologia , Relação Estrutura-AtividadeRESUMO
A new series of 4-substituted benzylideneamino-5[3(5)-methyl-5(3)-phenyl-1H-1-pyrazolyl]-2 ,4-dihydro-3H- 1,2,4-triazole-3-thione was synthesized by reaction of I with suitable aldehyde and tested for their antimicrobial activities against six microorganisms. Most of the compounds showed antibacterial activity against Gram positive bacteria.