Assuntos
Antígenos de Diferenciação de Linfócitos T/análise , Transplante de Coração , Linfócitos T/imunologia , Antígenos CD8 , Células Cultivadas , Rejeição de Enxerto , Antígenos de Histocompatibilidade Classe I/análise , Antígenos de Histocompatibilidade Classe II/análise , Humanos , Terapia de Imunossupressão , Miocárdio/imunologia , Linfócitos T/citologia , Linfócitos T Citotóxicos/imunologiaRESUMO
The effects of 2-deoxy-D-glucose (2DG), oligomycin and theophylline on the in vitro production and metabolism of glycerol and its response to insulin and epinephrine were studied in epididymal fat pads from fed rats. 2-DG failed to affect basal or epinephrine stimulated glycerol production but it decreased the uptake of 1-14 C-glycerol by the tissue and its conversion to glyceride-glycerol. Oligomycin also failed to affect the basal production of glycerol but it inhibited the effect of epinephrine on this parameter as well as the uptake and utilization of 1-14-C-glycerol. Theophylline enhanced the production of glycerol by the tissue and this effect was not further augmented by epinephrine. Theophyline also inhibited the uptake and utilization of 1-14C-glycerol; the most pronounced effect of theophylline was observed in the formation of 14C-fatty acids from 1-14C-glycerol in the presence of glucose. Insulin, but not epinephrine, decreased the inhibitory effect of theophylline on glycerol utilization. It is concluded that these compounds affect more intensely the ability of adipose tissue to metabolize glycerol than to release it through lipolysis. The pathway for glycerol utilization in adipose tissue appears to be more sensitive to changes in the availability of ATP than the mechanisms responsible for the release of glycerol from the tissue.
Assuntos
Tecido Adiposo/metabolismo , Desoxiaçúcares/farmacologia , Desoxiglucose/farmacologia , Epinefrina/farmacologia , Glicerol/metabolismo , Insulina/farmacologia , Oligomicinas/farmacologia , Teofilina/farmacologia , Tecido Adiposo/efeitos dos fármacos , Animais , Glucose/farmacologia , Técnicas In Vitro , Masculino , RatosRESUMO
From earlier studies, it appeared that postmenopausal women with osteoporosis may not be so responsive to therapeutic calcium infusions as younger men. A study of seven women with postmenopausal osteoporosis revealed that only one had a good therapeutic response as judged by sustained decrease in bone pain and improvement in calcium balance. It is concluded that a series of calcium infusions is unlikely to be therapeutically useful in postmenopausal osteoporosis.