Caseous Lymphadenitis Caused by Corynebacterium pseudotuberculosis in Alpine Chamois (Rupicapra r. rupicapra): a Review of 98 Cases.

Corynebacterium pseudotuberculosis is the causative agent of caseous lymphadenitis (CLA) in domestic and wild ruminants. Here we describe CLA in alpine chamois (Rupicapra r. rupicapra) based on a series of 98 cases of C. pseudotuberculosis infection confirmed by bacteriology and gene sequence analysis. The population included 53 males and 45 females distributed within three age groups: up to 18 months (n = 14), 18 months to 4 years (n = 11) and over 4 years (n = 73). Four different gross lesion distribution patterns, observed individually or variably combined in the same animal, were defined: (1) cutaneous/external (i.e. subcutaneous lymph nodes with or without muscle involvement, n = 34); (2) abdominal visceral (i.e. only abdominal organs involved: liver and/or spleen and/or kidney and/or lymph nodes, n = 35); (3) thoracic visceral (i.e. only thoracic organs involved: lung and/or heart and/or lymph nodes, n = 26); and (4) generalized visceral (i.e. abdominal and thoracic organs involved, n = 26). In six particularly severe cases, mammary gland, testis, vertebral bone and the central nervous system were also affected. Macroscopically, most abscesses were characterized by fluid pus, confirmed by microscopy that showed the absence of distinct concentric layers and coagulative necrosis, which are typically seen in sheep and goats raised in areas where the infection is endemic. In three cases amyloid deposits were observed in the liver and kidney. The C. pseudotuberculosis strains isolated were highly homologous to the reference strain ATCC 19410, except for some variability in their ability to ferment maltose and mannitol. Based on the production of nitrate reductase, 95 strains were attributed to the ovis biovar (nitrate reduction negative) and three to the equi biovar (nitrate reduction positive). All strains were sensitive to antibiotics, except to ampicillin (62.3% resistant strains) and gentamicin (83.7% resistant strains). Using an indirect enzyme-linked immunosorbent assay designed for CLA in sheep and goats, seven (58.3%) of 12 serum samples tested positive for antibodies.

Methicillin resistance in Staphylococcus aureus strains isolated from food and wild animal carcasses in Italy.

Following the detection of methicillin-resistant Staphylococcus aureus (MRSA) ST398 in food-producing animals, both livestock and wildlife, and derived products, are considered potential sources of MRSA in humans. There is a paucity of data on MRSA in foods in Italy, and the data regarding wild animals are particularly scarce. A total of 2162 food samples collected during official monitoring activities in 2008 were analyzed for the detection of S. aureus. Also, samples from 1365 wild animals collected by the National Reference Center for Wild Animal Diseases in 2003-2009 were subjected to anatomopathological examination. S. aureus isolates were processed for phenotypic and molecular methicillin resistance determinations. S. aureus was found in 2.0% of wild animal carcasses and in 3.2% of wild boar lymph nodes: none showed methicillin resistance. The prevalence of S. aureus in food was 17.1%. Two MRSA strains, both from bulk tank milk (prevalence 0.77%) were isolated: the strains were resistant to tetracycline, had spa-type t899, and were negative for the Panton-Valentine leukocidin gene. The low prevalence of MRSA suggests that the risk of transmission to humans via food is limited. However, attention should be paid to the cattle food chain, which may be a potential route of transmission of LA-MRSA.

Nocardia otitidiscaviarum pneumonia in an Alpine chamois (Rupicapra rupicapra rupicapra).

Nocardia otitidiscaviarum was cultured from the lung of an Alpine chamois (Rupicapra rupicapra rupicapra) with suppurative bronchopneumonia. This is the first report of both nocardiosis and Nocardia otitidiscaviarum in this wild ungulate species.