Integration of Basic Chemical Knowledge into the Key Undergraduate Course-Biochemistry / 中国生物化学与分子生物学报

The basic knowledge of college chemistry is an important basis for the key undergraduate course-Biochemistry for students majoring in Life Sciences. Based on theoretical analysis of the close relationship between "Biochemistry" and "College Chemistry", this paper summarized these knowledge nodes to the content of Biochemistry from the four major courses: Chemistry, Inorganic Chemistry, Organic Chemistry, Analytical Chemistry and Physical Chemistry. We took the peptide bond,mechanism of enzymes and protein purification as examples to show the relevance between the basic knowledge of Chemistry and the knowledge points of Biochemistry, aiming to enlighten the students to understand the importance of the basic knowledge of Chemistry. We also proposed three kinds of teaching pattern for introducing the basic knowledge of " College Chemistry" to " Biochemistry" , including " Described directly by teachers", " Integrated into contents of Biochemistry" and " Students learning by themselves" , so as to ensure that the students comprehend clearly the essence of "Biochemistry". Finally we discussed how to culture the students' ability to think independently and critically. The idea and practice about Biochemistry teaching will benefit the students to grasp the essence of the course and it will also give one new perspective for teachers to carry out teaching reforms in life science courses.

Research progress and application of MEEK micrografting technique / 解放军医学杂志

Wound coverage in early stage is the key of extensive burn treatment.Meek micro skin grafting technique plays an important role for wound coverage in extensive burn injury.There are several advantages by using the Meek technique,such as high expanding ratio,shorter operation time,lower incidence of infection,shorter time of repairing wound,lower cost of unit area repair.Meek technique is worth popularizing for these advantages in extensive burn wound repair,however,it could not replace other skin grafts methods.This article reviews the research progress and clinical application of Meek Technique.

Clinical application analysis and evaluation of 128-slice GEM CT technique / 医疗卫生装备

Objective To evaluate the clinical application of 128-slice GEM CT in order to analyze its advantages in clinical application.Methods The characteristics of ASiR technique were introduced,CT images of target area were obtained,and the clinical advantages of the technique were analyzed.Results The technique gained advantages in the imaging examination of heart,abdomen,soft tissues,vascular plaque and high resolutions in time,space and density,and image noise was decreased with no X-ray radiation dose increased.Conclusion The technique enhances CT examination in image quality,diagnosis accuracy,examination efficiency and high resolutions in time,space and density.

Clinical application analysis and evaluation of 128-slice GEM CT technique / 医疗卫生装备

Objective To evaluate the clinical application of 128-slice GEM CT in order to analyze its advantages in clinical application.Methods The characteristics of ASiR technique were introduced,CT images of target area were obtained,and the clinical advantages of the technique were analyzed.Results The technique gained advantages in the imaging examination of heart,abdomen,soft tissues,vascular plaque and high resolutions in time,space and density,and image noise was decreased with no X-ray radiation dose increased.Conclusion The technique enhances CT examination in image quality,diagnosis accuracy,examination efficiency and high resolutions in time,space and density.

Therapeutic effects of small interfering RNA targeting MDM2 on human osteosarcoma cells / 中华肿瘤杂志

<p><b>OBJECTIVE</b>To investigate the inhibitory effect of the small interfering RNA targeting mdm2 gene on the growth of osteosarcoma cells.</p><p><b>METHODS</b>PGCsilencerTM-mdm2 siRNA was constructed and transfected into the osteosarcoma cell line U2OS cells. The inhibitory effects on mdm2 were determined by RT-PCR and Western blot analysis. The cell growth activity was determined by MTT assay, and the cell apoptosis was examined by flow cytometry. The therapeutic effects of simdm2 was assessed on the nude mouse model of transplanted tumor.</p><p><b>RESULTS</b>The simdm2 plasmid was successfully constructed. After simdm2 being transfected into the U2OS cells, the expressions of mdm2 gene and protein were significantly inhibited. The ability of cell growth activity decreased greatly and cell apoptosis occurred apparently. There was no significant difference between the negative control group and non-transfected group. The growth of xenograft tumor in simdm2 transfected nude mice was inhibited and the expressions of mdm2 gene and protein were down-regulated remarkably.</p><p><b>CONCLUSION</b>siRNA targeting mdm2 gene inhibits the mdm2 expression in osteosarcoma U2OS cells and the growth of osteosarcoma in nude mice.</p>

Preparation of bovine lipid-free hemoglobin / 生物工程学报

A new method for preparation of Hb solution free of stromal lipid was described. Almost all the lipid in fresh hemolysate of bovine red blood was removed with hydrophobic interaction chromatography (HIC) in the presence of 2% PEG4000, 5% PEG4000, 2%PEG10000 or 5%PEG10000. With the adding of 5%PEG4000, the 80% of recovery of Hb in HIC was obtained and the maximum lipid absorbed by hydrophobic medium, butyl agarose -6B was 86.6 mg/mL. The activity (P50) of hemoglobin preparation was 3386.4 Pa torrs, and the Hill number was 2.54, which were near to that of the native red blood cells. The mechanism of removing lipid by HIC and the function of PEG in the process were discussed.