RESUMO
Knowledge, attitude, and practice (KAP) studies have recently been suggested as a useful tool to understand the specificity of the population related to a disease. However, in Cameroon, there is a lack of information based on KAP studies regarding obesity. This study has been designed to collect basic indicators on the KAP of the populations regarding overweight and obesity in urban and rural areas in Cameroon (Douala and Manjo). For this purpose, an epidemiological community-based cross-sectional descriptive study was conducted in these two areas using a well-structured questionnaire. Sociodemographic and medical characteristics and KAP information were assessed. For the quantification of KAP, a score varying from 0 (poor knowledge, attitude, or practices) to 100 (good knowledge, attitude, or practices) was attributed for each question. Correlations between knowledge, attitude, and practice were determined using inferential statistics tests which were χ2 test, independent Student t-test, ANOVA (followed by Tukey's post hoc test), and Pearson correlation coefficient. Results reveal that living in a rural area (Manjo), being overweight or obese, having complete secondary education, and being married increase the knowledge and the practice score. There is a strong and positive correlation between knowledge and practice score. However, there is no association between attitude and practice and between attitude and knowledge. Reducing the disparities between knowledge, attitude, and practices constitutes a serious track in a holistic strategy for the management of obesity in these areas.
RESUMO
Objective: The aim of this study was to determine the chemical characteristics and antibacterial activity of Fontitrygon margarita liver oil against the bacteria responsible for food poisoning. Methods: Oils were extracted from F. margarita liver using two methods (exudation and cooking-pressing) and analyses by Fourier transform infrared (FTIR) spectroscopy. Quality indexes were determined using standard methods and the fatty acid profile was carried out by gas chromatography with a flame ionization detector (GC-FID). Antibacterial activities of these oils, their emulsion, and their interactions with common antibiotics were evaluated by the broth microdilution method. Results: Extraction yield was higher with cooking-pressing (16.90%) compared to exudation (14.49%). The quality indexes of both oils were conformed to Codex Alimentarius Standard. Thiobarbituric acid index was higher with exudation compared to cooking-pressing (3.20 ± 0.14 and 2.36 ± 0.14 µmol MDA/kg, respectively) while acid, iodine, peroxide, and anisidine values did not significantly vary with the extraction methods (2.15-2.30 mgKOH/g, 102.42-106.65 gI2/100 g, 3.34-3.57 meqO2/kg, and 2.85-3.32 respectively). FTIR analyses clearly show that the two spectra are similar (no differences in the frequency and absorbance of their bands). The fatty acid profile revealed that, regardless of the extraction methods, F. margarita oil is richer in monounsaturated (55.97-55.41%) followed by polyunsaturated (28.17-28.52%) and saturated fatty acids (15.86-16.07%). Moreover, these oils showed antibacterial activity on all the bacteria strains tested with MICs between 16 and 256 mg/ml. Regardless of the extraction methods, emulsions showed higher activity (6.25 ≤ MIC ≤25 mg/ml) compared to crude oils. Additionally, F. margarita liver oil potentiated the antibacterial activity of ciprofloxacin, tetracycline, gentamicin, amoxicillin, and chloramphenicol. Conclusion: These results showed the effectiveness of Fontitrygon margarita liver oil against some bacteria responsible for food poisoning, thus demonstrating their antibacterial properties which could be due to their chemical composition.
RESUMO
An international survey among consumers in developing countries from Asia and Africa on food safety knowledge, attitudes and practices (KAP) was carried out using structured questionnaires. Data were collected from 453 consumers comprising 265 from Africa and 188 from Asia. Significant difference (pâ¯<â¯0.05) on food safety knowledge attitude and practice between consumers from Africa and Asia was observed. In Africa, consumers in Cameroon had the least food safety knowledge (73.15⯱â¯16.43) compared to Ghana (78.19⯱â¯15.84) and Nigeria (88.16⯱â¯8.88). Similarly, consumers in Iran had the least food safety knowledge (73.33⯱â¯19.84) in Asia compared to Malaysia (88.36⯱â¯11.64) and Pakistan (89.42⯱â¯9.89). Among the respondents, 89% were aware of food poisoning while 304 (67.1%) consume food kept for long at room temperature. There was significant difference (pâ¯<â¯0.05) in food safety knowledge, attitudes and practices of consumers between Africa and Asia. Overall, respondents from Asia have better food safety knowledge than respondents from Africa.
