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1.
Bratisl Lek Listy ; 108(9): 414-6, 2007.
Artigo em Inglês | MEDLINE | ID: mdl-18225481

RESUMO

Neurocysticercosis (cysticercosis cerebri) is a rare neurological diagnosis in Croatia. It is classically divided into four types: intraparenchymal, basilar cisternal, ventricular and diffuse. Computerized tomography (CT) and magnetic resonance imaging (MRI) established the diagnosis by demonstrating cysticercosis disseminated throughout the cerebral parenchyma. The authors emphasize the potential of the ELISA test to detect anticysticercosis antibodies in blood and cerebrospinal liquor (CSF). Diagnostics of neurocysticercosis is enabled jointly by clinical signs, neuroradiological, serum and liquor tests. We report the case of a 70-year old man with clinical and neuroradiological signs of cysticercosis cerebri. The neurological status is dominated by ataxia, corticospinal pathways damages and cognitive capacity impairments. CT of the brain shows calcificated and cystic lesions of various sizes. MRI of the brain enables the final diagnosis of the cysticercosis cerebri with multiple and multicentric lesions that indicate various stages of the cerebral cysticercosis (Fig. 2, Ref 20). Full Text (Free, PDF) www.bmj.sk.


Assuntos
Neurocisticercose/diagnóstico , Idoso , Humanos , Masculino , Neurocisticercose/diagnóstico por imagem , Tomografia Computadorizada por Raios X
2.
Folia Microbiol (Praha) ; 47(4): 458-60, 2002.
Artigo em Inglês | MEDLINE | ID: mdl-12422529

RESUMO

The calcium hydroxide-based filling material Apexit, which is often used in endodontic practice, was evaluated for its antibacterial and antifungal effects against microorganisms isolated from oral cavity (Serratia marcescens, Pseudomonas putida, Staphylococcus aureus, Candida albicans). Two different quantitative techniques were employed--the direct-contact test was used to examine the efficacy of freshly mixed material while the broth-survival test was employed to check the antimicrobial properties of 5-d-old material. Apexit inhibited Gram-negative bacteria more effectively than Gram-positive ones but had none or a very weak inhibitory effect on C. albicans.


Assuntos
Bactérias/efeitos dos fármacos , Hidróxido de Cálcio/farmacologia , Materiais Restauradores do Canal Radicular/farmacologia , Antibacterianos , Anti-Infecciosos/farmacologia , Bactérias/crescimento & desenvolvimento , Bactérias/isolamento & purificação , Candida albicans/efeitos dos fármacos , Candida albicans/crescimento & desenvolvimento , Humanos , Técnicas In Vitro , Boca/microbiologia , Pseudomonas putida/efeitos dos fármacos , Pseudomonas putida/crescimento & desenvolvimento , Tratamento do Canal Radicular , Serratia marcescens/efeitos dos fármacos , Serratia marcescens/crescimento & desenvolvimento , Staphylococcus aureus/efeitos dos fármacos , Staphylococcus aureus/crescimento & desenvolvimento
3.
Infect Immun ; 69(4): 2569-79, 2001 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-11254621

RESUMO

Legionella pneumophila replicates within alveolar macrophages, and possibly, alveolar epithelial cells and also within protozoa in the aquatic environment. Here we characterize an L. pneumophila mutant defective in the HtrA/DegP stress-induced protease/chaperone homologue and show that HtrA is indispensable for intracellular replication within mammalian macrophages and alveolar epithelial cells and for intrapulmonary replication in A/J mice. Importantly, amino acid substitutions of two conserved residues in the catalytic domain of (H103mapstoR and S212mapstoA) and in-frame deletions of either or both of the two conserved PDZ domains of HtrA abolish its function. Interestingly, the htrA mutant exhibits a parental-type phenotype in intracellular replication within the protozoan host Acanthamoeba polyphaga. We used a promoterless lacZ fusion to the htrA promoter to probe the phagosomal microenvironment harboring L. pneumophila within macrophages and within A. polyphaga for the exposure to stress stimuli. The data show that expression through the htrA promoter is induced by 12,000- to 20,000-fold throughout the intracellular infection of macrophages but its induction is by 120- to 500-fold within protozoa compared to in vitro expression. Data derived from confocal laser scanning microscopy reveal that in contrast to the parental strain, phagosomes harboring the htrA mutant within U937 macrophages colocalize with the late endosomal-lysosomal marker LAMP-2, similar to killed L. pneumophila. Coinfection experiments examined by confocal laser scanning microscopy show that in communal phagosomes harboring both the parental strain and the htrA mutant, replication of the mutant is not rescued, while replication of a dotA mutant control, which is normally trafficked into a phagolysosome, is rescued by the parental strain. Our data show, for the first time, that the stress response by L. pneumophila (mediated, at least in part, by HtrA) is indispensable for intracellular replication within mammalian but not protozoan cells.


Assuntos
Eucariotos/microbiologia , Proteínas de Choque Térmico , Legionella pneumophila/crescimento & desenvolvimento , Proteínas Periplásmicas , Serina Endopeptidases/fisiologia , Animais , Linhagem Celular , Humanos , Legionella pneumophila/genética , Pulmão/microbiologia , Macrófagos/microbiologia , Camundongos , Fases de Leitura Aberta , Serina Endopeptidases/genética
4.
Infect Immun ; 68(11): 6431-40, 2000 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-11035756

RESUMO

Legionella pneumophila does not induce apoptosis in the protozoan host, but induces pore formation-mediated cytolysis after termination of intracellular replication (L.-Y. Gao and Y. Abu Kwaik, Environ. Microbiol. 2:79-90, 2000). In contrast to this single mode of killing of protozoa, we have recently proposed a biphasic model by which L. pneumophila kills macrophages, in which the first phase is manifested through the induction of apoptosis during early stages of the infection, followed by an independent and temporal induction of necrosis during late stages of intracellular replication. Here we show that, similar to the protozoan host, the induction of necrosis and cytolysis of macrophages by L. pneumophila is mediated by the pore-forming toxin or activity. This activity is temporally and maximally expressed only upon termination of bacterial replication and correlates with cytolysis of macrophages and alveolar epithelial cells in vitro. We have identified five L. pneumophila mutants defective in the pore-forming activity. The phagosomes harboring the mutants do not colocalize with the late endosomal or lysosomal marker Lamp-1, and the mutants replicate intracellularly similar to the parental strain. Interestingly, despite their prolific intracellular replication, the mutants are defective in cytotoxicity and are "trapped" within and fail to lyse and egress from macrophages and alveolar epithelial cells upon termination of intracellular replication. However, the mutants are subsequently released from the host cell, most likely due to apoptotic death of the host cell. Data derived from cytotoxicity assays, confocal laser scanning microscopy, and electron microscopy confirm the defect in the mutants to induce necrosis of macrophages and the failure to egress from the host cell. Importantly, the mutants are completely defective in acute lethality (24 to 48 h) to intratracheally inoculated A/J mice. We conclude that the pore-forming activity of L. pneumophila is not required for phagosomal trafficking or for intracellular replication. This activity is expressed upon termination of bacterial replication and is essential to induce cytolysis of infected macrophages to allow egress of intracellular bacteria. In addition, this activity plays a major role in pulmonary immunopathology in vivo.


Assuntos
Antígenos de Bactérias , Legionella pneumophila/patogenicidade , Macrófagos/patologia , Alvéolos Pulmonares/patologia , Animais , Apoptose , Proteínas de Bactérias/fisiologia , Feminino , Humanos , Proteínas de Membrana/fisiologia , Camundongos , Necrose , Células U937
5.
Mediators Inflamm ; 9(5): 229-34, 2000.
Artigo em Inglês | MEDLINE | ID: mdl-11200363

RESUMO

BACKGROUND: Exposure to microorganisms elicts the production of cytokines. These soluble factors enhance several innate immune functions and regulate the ensuing specific immune response aimed at limiting the spread of infection. AIM: This study was undertaken to quantify the plasma levels of pro-inflammatory cytokines during the course of primary Listeria monocytogenes and Campylobacter jejuni infection. Using an in vivo infection the relationship between endogenous cytokines and the bacterial number in the liver of infected animals was examined. METHODS: C57BL/6 mice were infected by the intraperitoneal route. At different time points we determined the number of colony-forming units of bacteria in the liver of infected animals and paralled these with the plasma levels of interferon-gamma (IFN-gamma), tumor necrosis factor-alpha (TNF-alpha) and interleukin-6 (IL-6) measured by enzyme immunoassays. RESULTS: L. monocytogenes infection lasted 10-11 days. IFN-gamma production occurred in the early phase but was more pronounced after day 4, following the appearance of specific immunity. The duration of experimental campylobacteriosis was 15 days. Early IFN-gamma production was not significant but a progressive rise of this cytokine in plasma was seen during the second week post infection. Mice produced measurable amounts of plasma TNF-alpha immediately after being given viable L. monocytogenes, peaking on day 2-3 when the greatest number of bacteria was present in the examined organs. During C. jejuni infection plasma TNF-alpha was produced in a similar manner, but the highest concentrations were found a few days later than in listeriosis, in correlation with the different course of campylobacteriosis. The quantity of IL-6 increased and decreased in concordance with clearance of L monocytogenes and the clinical status of the animals. C. jejuni did not promote the induction of this cytokine. This is to some extent an unusual finding. With respect to the role of IL-6 in Th2 responses and antibody production, the appearance of this cytokine in campylobacteriosis was more expected. DISCUSSION: During systemic bacterial infection, a network of pro-inflammatory cytokines is activated and blood levels of these cytokines are elevated, albeit inconsistently, with large individual variations and depending on microbial characteristics and structure.


Assuntos
Infecções por Campylobacter/imunologia , Campylobacter jejuni/imunologia , Interferon gama/sangue , Interleucina-6/sangue , Listeriose/imunologia , Fator de Necrose Tumoral alfa/análise , Animais , Infecções por Campylobacter/sangue , Infecções por Campylobacter/microbiologia , Feminino , Listeria monocytogenes/imunologia , Listeriose/sangue , Listeriose/microbiologia , Fígado/imunologia , Fígado/microbiologia , Masculino , Camundongos , Camundongos Endogâmicos C57BL
6.
Microb Pathog ; 24(4): 263-8, 1998 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-9533898

RESUMO

Campylobacter jejuni is one of the most frequent causes of diarrhoea in man. Extra-intestinal manifestations may also occur, particularly in immunocompromised individuals. However, because of the lack of appropriate animal models the pathophysiology and immunological response of the host to C. jejuni infection are still poorly understood. In our laboratory an experimental infection of adult BALB/c, C57BL/6 and DBA/2 mice has been established. After intraperitoneal injection of 0.5-1x10(9) cfu of C. jejuni none of the infected mice showed clinical symptoms of illness, but bacterial spreading and tissue invasion were achieved. We have concentrated our studies on the duration of primary infection, recovery of bacteria from livers and spleens of infected animals and pathohistological changes of these organs. Our results showed differences in the course of systemic infection among the tested mice strains. BALB/c mice were most sensitive, resulting in the most pronounced pathohistological changes in the examined organs. The duration of the primary liver infection was the longest in BALB/c mice while the duration of the splenic infection also differed among the tested mice strains. Nevertheless, the experimental model used in this study can be efficiently used in further analysis of the pathogenesis of this bacterial infection. However, the strain differences should be taken into account depending on the parameters to be followed.


Assuntos
Infecções por Campylobacter/microbiologia , Campylobacter jejuni/crescimento & desenvolvimento , Modelos Animais de Doenças , Camundongos Endogâmicos/microbiologia , Animais , Infecções por Campylobacter/patologia , Feminino , Fígado/microbiologia , Fígado/patologia , Masculino , Camundongos , Baço/microbiologia , Baço/patologia
7.
J Immunol ; 160(1): 316-21, 1998 Jan 01.
Artigo em Inglês | MEDLINE | ID: mdl-9551986

RESUMO

The inflammatory response and influence of T cell depletion on the pathogenesis of an experimental Legionella infection were studied. A/J mice were infected with 10(6) CFU of Legionella pneumophila intratracheally. With this dose all infected animals survived the infection and bacteria were cleared from lung, spleen, liver, and kidney within 10 to 11 days, leaving no residual changes in the affected organs. Inflammatory cells were recruited into the lung on the second day of infection, reaching a maximum on the third day and filling out predominantly the interstitial areas. During the first 3 days after inoculation, mainly macrophages, B cells, NK cells, and large mononuclear cells of an unknown phenotype were attracted into the lung interstitium, whereas T lymphocytes infiltrated subsequently. During the early phase of infection, serum concentrations of IFN-gamma, TNF-alpha, IL-1beta, IL-4, and IL-6 but not IL-2 increased dramatically. The cytokine secretion decreased on the third day after infection although bacteria were still present in the lung or even disseminated in different organs. Successful clearance of bacteria from the lung was not observed before recruitment of T cells into the lung. In mice depleted of both CD4+ and CD8+ T cells, control of infection was impaired and lethality of infection increased. Depletion of either subset left residual antibacterial mechanisms, which, however, were not sufficient to clear the Legionella as rapidly as in undepleted mice.


Assuntos
Linfócitos T CD4-Positivos/imunologia , Linfócitos T CD8-Positivos/imunologia , Legionella pneumophila/imunologia , Doença dos Legionários/imunologia , Animais , Citocinas/biossíntese , Feminino , Hospedeiro Imunocomprometido , Legionella pneumophila/crescimento & desenvolvimento , Pulmão/imunologia , Pulmão/microbiologia , Pulmão/patologia , Depleção Linfocítica , Camundongos , Camundongos Endogâmicos A
8.
Infect Immun ; 65(5): 1754-60, 1997 May.
Artigo em Inglês | MEDLINE | ID: mdl-9125558

RESUMO

An O-antigen-specific murine monoclonal antibody (MAb) directed against an immunodominant epitope expressed on Klebsiella O1, O6, and O8 lipopolysaccharides (LPS) was examined with respect to its binding to nonencapsulated and encapsulated bacterial cells and its ability to protect against lethal murine Klebsiella sepsis. While the MAb (clone Ru-O1, mouse immunoglobulin G2b) bound well to nonencapsulated organisms of the O1 serogroup, binding was significantly, but not completely, abolished by the presence of the K2 capsule. In a model of experimental Klebsiella peritonitis and sepsis induced by a virulent O1:K2 serogroup strain, higher doses of anti-LPS MAb Ru-O1 than of a previously described anticapsular MAb specific for the K2 capsular polysaccharide were needed to provide protection. However, high-dose (40 microg/g of body weight) pretreatment with anti-LPS MAb Ru-O1 significantly reduced bacterial dissemination to various organs as well as macroscopic and histologic pulmonary alterations. Thus, since the number of Klebsiella capsular antigens occurring in clinical material is too large to be completely "covered" by a K-antigen-specific hyperimmunoglobulin preparation, O-antigen-specific antibodies may supplement K-antigen-specific immunoprophylaxis and -therapy of clinical Klebsiella infection.


Assuntos
Anticorpos Antibacterianos/imunologia , Anticorpos Antibacterianos/farmacologia , Anticorpos Monoclonais/imunologia , Anticorpos Monoclonais/farmacologia , Infecções por Klebsiella/imunologia , Infecções por Klebsiella/prevenção & controle , Lipopolissacarídeos/imunologia , Antígenos O/imunologia , Antígenos O/farmacologia , Animais , Afinidade de Anticorpos , Contagem de Colônia Microbiana , Ensaio de Imunoadsorção Enzimática , Epitopos/imunologia , Imunoterapia , Klebsiella/imunologia , Klebsiella/patogenicidade , Fígado/microbiologia , Pulmão/microbiologia , Pulmão/patologia , Masculino , Camundongos , Camundongos Endogâmicos BALB C , Peritonite/imunologia , Peritonite/microbiologia , Sepse/imunologia , Sepse/microbiologia , Organismos Livres de Patógenos Específicos , Baço/microbiologia
9.
Folia Biol (Praha) ; 43(1): 33-8, 1997.
Artigo em Inglês | MEDLINE | ID: mdl-9158947

RESUMO

Antibiotics, in addition to killing or inhibiting the growth of microorganisms, may also affect the mechanism of host defence in many ways. Such effects may be clinically relevant especially in the case where an impairment of immunological function can be seen. We, therefore, decided to study the influence of penicillin G, cefotaxim, ceftazidime, streptomycin, and lincomycin on the function of phagocytes by using the macrophage adherence assay and the macrophage spreading assay. We also followed the concentrations of neopterin and interferon gamma (IFN gamma) in the plasma of mice treated with the above mentioned antibiotics. Changes in adherence of peritoneal macrophages were seen in mice treated with therapeutic doses of penicillin G and cefotaxim, after 2 h of incubation. Cefotaxim and streptomycin in the usual therapeutic dose and ceftazidime in a fourfold higher dose influenced the capacity of peritoneal macrophages to spread on a glass surface. The same was seen with lincomycin when administered in the therapeutic dose and in a fourfold higher dose. In all the mice treated with antibiotics the concentration of IFN gamma was higher than in the control mice, while the reverse was seen concerning neopterin release, with an exception in mice treated with streptomycin.


Assuntos
Antibacterianos/farmacologia , Macrófagos Peritoneais/fisiologia , Animais , Biopterinas/análogos & derivados , Biopterinas/biossíntese , Cefotaxima/farmacologia , Ceftazidima/farmacologia , Adesão Celular/efeitos dos fármacos , Movimento Celular/efeitos dos fármacos , Feminino , Interferon gama/biossíntese , Lincomicina/farmacologia , Macrófagos Peritoneais/efeitos dos fármacos , Masculino , Camundongos , Camundongos Endogâmicos BALB C , Camundongos Endogâmicos CBA , Camundongos Endogâmicos DBA , Camundongos Endogâmicos , Neopterina , Penicilina G/farmacologia , Estreptomicina/farmacologia
10.
Folia Microbiol (Praha) ; 42(1): 65-71, 1997.
Artigo em Inglês | MEDLINE | ID: mdl-9161004

RESUMO

The facultative intracellular Gram-positive bacterium Listeria monocytogenes is a food-borne pathogen of frequently underestimated importance. Pregnant women represent the high-risk group for L. monocytogenes infection. Abortion, stillbirth or neonatal infection can be the serious outcome of such an infection. Recovery from listeriosis, resistance mechanisms of the host and the effect of L. monocytogenes on fetal development still remain to be fully understood. The results of our experiments showed an increased susceptibility of gestating BALB/c mice to primary L. monocytogenes infection. The duration of listeriosis in gestating animals was almost twice longer than in the control group. Furthermore, it was clearly shown that the detrimental effect of L. monocytogenes on fetal development was more pronounced if the infection was acquired earlier during gestation.


Assuntos
Listeriose/complicações , Complicações Infecciosas na Gravidez , Animais , Desenvolvimento Embrionário e Fetal , Feminino , Reabsorção do Feto/etiologia , Reabsorção do Feto/microbiologia , Reabsorção do Feto/patologia , Idade Gestacional , Humanos , Listeria monocytogenes/isolamento & purificação , Listeria monocytogenes/patogenicidade , Listeriose/etiologia , Listeriose/patologia , Depleção Linfocítica , Camundongos , Camundongos Endogâmicos BALB C , Placenta/microbiologia , Placenta/patologia , Gravidez , Complicações Infecciosas na Gravidez/etiologia , Complicações Infecciosas na Gravidez/microbiologia , Complicações Infecciosas na Gravidez/patologia , Subpopulações de Linfócitos T/imunologia
11.
Med Arh ; 47(1-2): 41-2, 1993.
Artigo em Servo-Croata (Latino) | MEDLINE | ID: mdl-7934223

RESUMO

A case of the acute haemorrhaging ulcus in a three-months old infant with lethal outcome is presented in this paper. The unclear proximal gastrointestinal haemorrhage didn't indicate the possibility of the presence of ulcer, due to the absence of frequent etiologic factors of the inception at this age.


Assuntos
Úlcera Péptica Hemorrágica , Doença Aguda , Feminino , Humanos , Lactente , Úlcera Péptica Hemorrágica/diagnóstico , Úlcera Péptica Hemorrágica/patologia
12.
Folia Biol (Praha) ; 39(3): 162-5, 1993.
Artigo em Inglês | MEDLINE | ID: mdl-8157137

RESUMO

Antibiotics, in addition to killing or inhibiting the growth of microorganisms, may also affect the immune response in many ways. Such effects may be clinically relevant especially when an impairment of immunological functions is seen. This study was designed to analyse the influence of various groups of antibiotics on the host defence system by using different tests in vitro on human or animal cells and in vivo on an animal model. At the same time we analysed the antimicrobial effectiveness of the same antibiotics on over 2800 bacterial strains isolated from our clinical material. Preliminary findings show that a correlation exists between the effect of antibiotics on the immune response, especially the humoral response, and the appearance of resistance in bacteria to these antibiotics.


Assuntos
Antibacterianos/farmacologia , Bactérias/efeitos dos fármacos , Sistema Imunitário/efeitos dos fármacos , Animais , Formação de Anticorpos/efeitos dos fármacos , Resistência Microbiana a Medicamentos , Humanos , Linfócitos/imunologia , Camundongos , Testes de Sensibilidade Microbiana , Baço/imunologia
13.
Folia Biol (Praha) ; 37(2): 131-3, 1991.
Artigo em Inglês | MEDLINE | ID: mdl-1874340

RESUMO

In medical practice aryloxycarbonic acid derivatives have been solely used in the treatment of hyperlipidemia. Recently we patented the use of some of these derivatives in the treatment of dermatological diseases. Further analyses showed that these substances stimulated various cells of mesodermal origin including lymphocytes, the principal cells of the immune system. Depending on the concentration some derivatives of aryloxycarbonic acid stimulated or suppressed the proliferative response of lymphocytes, pointing to the possible use of these drugs as immunomodulators in clinical practice as well.


Assuntos
Anticolesterolemiantes/farmacologia , Bezafibrato/farmacologia , Doenças do Tecido Conjuntivo/tratamento farmacológico , Ativação Linfocitária/efeitos dos fármacos , Anticolesterolemiantes/uso terapêutico , Bezafibrato/uso terapêutico , Clofibrato/farmacologia , Clofibrato/uso terapêutico , Fenofibrato/farmacologia , Fenofibrato/uso terapêutico , Humanos , Fito-Hemaglutininas/farmacologia
14.
Jugosl Ginekol Perinatol ; 31(1-2): 37-9, 1991.
Artigo em Servo-Croata (Latino) | MEDLINE | ID: mdl-1875721

RESUMO

The primary malignant melanoma of the ovary is a rare kind of neoplasm. This one is in the world literature the eleventh well-documented case having developed in the ovary of a 66-year-old woman. A unilateral ovarian teratoma was removed, within which a malignant melanoma was found. The junctional activity of melanocytes was demonstrated in the epidermis within the teratoma. An extraovarian primary melanoma was not observed. The patient was treated with a bilateral adnexectomy and hysterectomy.


Assuntos
Cisto Dermoide/patologia , Melanoma/patologia , Neoplasias Primárias Múltiplas/patologia , Neoplasias Ovarianas/patologia , Idoso , Feminino , Humanos
15.
Folia Biol (Praha) ; 35(3): 152-5, 1989.
Artigo em Inglês | MEDLINE | ID: mdl-2673847

RESUMO

The aim of this work was to contribute to identifying more precise diagnostic criteria of particular collagenosis. In a group of 215 patients with clinical diagnosis of collagenosis immunocomplex deposits at the dermal-epidermal junction were determined. The results, when compared with the clinical and histopathological findings, showed that direct immunofluorescence of skin sections of patients with collagenosis confirms the clinical diagnosis significantly more often than does the histopathologic analysis. The advantage of this test suggests that it should be used as an additional diagnostic method for confirming systemic diseases of the collagenous tissue.


Assuntos
Complexo Antígeno-Anticorpo/análise , Doenças do Colágeno/diagnóstico , Pele/imunologia , Adolescente , Adulto , Idoso , Criança , Pré-Escolar , Doenças do Colágeno/imunologia , Doenças do Colágeno/patologia , Feminino , Imunofluorescência , Histocitoquímica , Humanos , Lactente , Masculino , Pessoa de Meia-Idade
16.
Immunobiology ; 175(3): 236-44, 1987 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-2960609

RESUMO

Primiparous and multiparous mice, either syngeneically or allogeneically pregnant, were sacrificed at various stages of pregnancy, and the immune reactivity of cells from different lymphatic organs was analyzed by mixed lymphocyte reaction (MLR) and concanavalin A (Con A)-induced lymphocyte proliferation. In the MLR, spleen cells and cells from the axillary lymph nodes of pregnant animals showed similar changes in their response to allogeneic cells during the course of gestation. In comparison to age-matched virgin controls they had an increased reactivity between the 7th and 11th day of pregnancy. During the preimplantation period and the last week of pregnancy, their alloreactivity was comparable to that of the controls. Cells from the para-aortic lymph nodes, which drain the uterus, also showed an increased reactivity at mid-gestation, but in the preimplantation period and third week of pregnancy their alloreactivity was even decreased in comparison to the control animals. When T cell immunocompetence was measured by means of lymphocyte transformation induced with Con A, the pattern of reactivity was completely different. Lymphoid cells from all the above-mentioned tissues showed the highest response to Con A during the preimplantation period, constantly weakening towards term.


Assuntos
Ativação Linfocitária , Prenhez/imunologia , Animais , Concanavalina A/farmacologia , Feminino , Técnicas In Vitro , Teste de Cultura Mista de Linfócitos , Tecido Linfoide/imunologia , Camundongos , Camundongos Endogâmicos , Gravidez , Fatores de Tempo
17.
Immunobiology ; 172(1-2): 92-8, 1986 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-3770802

RESUMO

The rate of appearance of cells forming 19S hemolytic antibody (PFC) in the spleens of F1 newborn mice after adoptive anti-paternal immunization of fetuses, newborns and/or their mothers during pregnancy has been studied. An increase in the number of PFC was found at the age of 6 to 11 days in offspring of adoptively immunized mothers. These newborns, even when foster nursed by untreated mothers, still had a significantly higher number of PFC in comparison to the controls. In contrast, adoptive immunization of the newborns themselves resulted in a decrease of PFC during the second week after birth. Several possible explanations for the obtained results are discussed including the putative transplacental passage of immunocompetent cells.


Assuntos
Reação Enxerto-Hospedeiro , Imunidade Materno-Adquirida , Animais , Animais Recém-Nascidos/imunologia , Animais Lactentes/imunologia , Feminino , Imunização Passiva , Masculino , Camundongos , Camundongos Endogâmicos BALB C , Camundongos Endogâmicos CBA , Gravidez
18.
Cell Immunol ; 89(2): 355-64, 1984 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-6542455

RESUMO

Mouse placental extracts (PE) and corresponding Sephadex G-200 fractions were administered to isogeneic CBA mice along with an optimal immunizing dose of SRBC. Spleen cells were harvested 8 days later and transferred to CBA recipients, subsequently immunized with SRBC. The immunoregulatory activity of spleen cells from PE-treated donors was compared to cells from liver extract (LE)-treated controls or from mice immunized with SRBC only, using Cunningham's PFC direct and indirect tests. Within the dose range used, selective modulatory activities were obtained with cells from PE, but not from LE, treated mice, the latter being comparable to cell transfer effects from donors immunized with SRBC only. Spleen cells from animals injected with low doses of PE (0.25 to 4 mg per mouse) added to immunizing SRBC had a suppressive effect on the primary IgM response of recipients immunized against SRBC. In contrast, when SRBC were given to donor animals with higher doses of PE (8 to 13 mg), transferred spleen cells potentiated the IgM response of the recipients. These opposite suppressive and potentiating activities were found in distinct Sephadex G-200 fractions of 40 and 60 kDa, respectively. When the effect of PE treatment was tested within the same animal, the indirect secondary PFC response following a challenge with SRBC was significantly modified. We observed an overall suppression of the different isotypes after treatment with lower doses of PE or with its 40-kDa fraction. PE doses of 0.5 to 2 mg resulted in a stronger inhibition of IgM than IgG1 production. This phenomenon was also obtained with the 40 KDa fraction. IgG2 responses were significantly reduced by all doses of this fraction. In contrast, all doses of the 60-kDa fraction gave a strong stimulation of IgG2 and IgM responses and a constant suppression of the IgG1 response. This shows a clear dissociation between IgG1 and C'-fixing (IgM, IgG2) antibody classes as far as the influence of placental substances is concerned in their regulation. These data emphasize the relevance of isogeneic placental products as a useful physiological material capable of modulating xenogeneic immune responses (as well as allogeneic systems).


Assuntos
Formação de Anticorpos , Eritrócitos/imunologia , Placenta/imunologia , Extratos de Tecidos/imunologia , Animais , Cromatografia em Gel , Feminino , Humanos , Imunoglobulina G/análise , Imunoglobulinas/análise , Terapia de Imunossupressão , Camundongos , Camundongos Endogâmicos CBA , Gravidez , Ovinos , Baço/imunologia
19.
J Reprod Immunol ; 6(3): 187-95, 1984 May.
Artigo em Inglês | MEDLINE | ID: mdl-6737370

RESUMO

The immune reactivity of lymphoid cells from pregnant mice was studied during the course of pregnancy in primiparous and multiparous animals either " isopregnant " (male and female of same strain) or " allopregnant " (male and female differing at H-2), using a local GVH assay (CBA lymphoid cells injected into (CBA X A/J)F1 recipients). The findings were as follows: The lymphoid cell number in the para-aortic lymph nodes ( PALN ) was increased at all stages of gestation. The peak occurred in the 2nd week in primiparity and as early as 60 h after fertilization in multiparity. PALN cell alloreactivity was weak at the beginning and higher than normal in the third week of pregnancy. Spleen cell alloreactivity was increased in the second week and decreased in the third week in primiparous compared with multiparous animals. Anti-paternal alloreactivity exhibited by spleen cells of allogestation was decreased (as compared to cells of isogestation ) especially in primiparous mice, particularly in the third week. At this time, the anti-paternal alloreactivity of PALN cells was increased. The influence of the recipient's sex on GVHR intensity was reversed when the cells were obtained from a pregnant donor, becoming stronger in male compared with female hybrids.


Assuntos
Reação Enxerto-Hospedeiro , Linfócitos/imunologia , Prenhez , Animais , Feminino , Linfonodos/citologia , Transfusão de Linfócitos , Masculino , Camundongos , Camundongos Endogâmicos A , Camundongos Endogâmicos CBA , Gravidez , Fatores Sexuais , Baço/citologia , Transplante Homólogo
20.
Acta Neurol Scand ; 69(3): 182-5, 1984 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-6609518

RESUMO

Lymphocyte subpopulations (total T cells, active T cells and B cells) were simultaneously analyzed in peripheral blood and CSF of MS patients. All patients were in active disease, 3 to 4 weeks after first signs of disease activation appeared. Per cent levels and absolute numbers of examined lymphocyte subpopulations in the blood of MS patients were significantly lower than in healthy controls. In MS, the level of active T lymphocytes was lower in CSF than in peripheral blood. The results support our earlier observations relating to the role of active T lymphocytes in the clinical course of disease.


Assuntos
Linfócitos B/imunologia , Contagem de Leucócitos , Esclerose Múltipla/imunologia , Linfócitos T/imunologia , Adulto , Líquido Cefalorraquidiano/citologia , Feminino , Humanos , Masculino , Pessoa de Meia-Idade
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