RESUMO
We evaluated a rapid immunoperoxidase technique for the detection of cytomegalovirus (CMV) antigenemia in peripheral blood neutrophils of 56 transplant recipients (117 specimens) and 36 persons with AIDS (59 specimens). Antigenemia was 92% sensitive and 98% specific for the detection of clinical CMV infection in transplant recipients and 100% sensitive and 86% specific in persons with AIDS. Overall, CMV antigenemia was a more rapid and sensitive method for the detection of clinical CMV infection than either shell vial culture or conventional tube culture of blood.
Assuntos
Síndrome da Imunodeficiência Adquirida/complicações , Antígenos Virais/sangue , Infecções por Citomegalovirus/microbiologia , Citomegalovirus/imunologia , Transplante de Órgãos/efeitos adversos , Infecções por Citomegalovirus/complicações , Humanos , Neutrófilos/microbiologiaRESUMO
A tracheal aspirate from which parainfluenza 2 (PI-2) virus but not chlamydiae was isolated demonstrated positive immunofluorescence of elementary body- and reticulate body-like particles on direct examination with anti-chlamydia monoclonal antibodies (Syva Co.). In subsequent studies, we found this reagent to show specific staining of this strain of PI-2 as well as of 3 of 18 additional PI-2 strains that were evaluated.
Assuntos
Antígenos Virais/análise , Chlamydia trachomatis/imunologia , Vírus da Parainfluenza 2 Humana/imunologia , Respirovirus/imunologia , Linhagem Celular , Reações Cruzadas , Epitopos/análise , Imunofluorescência , Humanos , Traqueia/microbiologiaRESUMO
We evaluated epidemiologic features of 54 patients with measles seen at Wyler Children's Hospital during a 1983 measles epidemic in Chicago. Forty-one (76%) were less than 5 years old, and 23 (43%) were less than 16 months old. We found evidence of a low rate of measles immunity in preschool-aged children of immunization age immediately before the epidemic; only 62% of 173 emergency room patients this age had antibody on enzyme-linked immunosorbent assay. These observations, together with failure of school-based control measures to significantly affect the observed course of the epidemic, indicate that the preschool population sustained the epidemic. Unimmunized preschool-aged children are important in the current epidemiology of measles, and improved immunization strategies are needed to control measles in this population in Chicago and perhaps other cities.