Modulation of Cellular Colonization of Porous Polyurethane Scaffolds via the Control of Pore Interconnection Size and Nanoscale Surface Modifications.

Full-scale cell penetration within porous scaffolds is required to obtain functional connective tissue components in tissue engineering applications. For this aim, we produced porous polyurethane structures with well-controlled pore and interconnection sizes. Although the influence of the pore size on cellular behavior is widely studied, we focused on the impact of the size of the interconnections on the colonization by NIH 3T3 fibroblasts and Wharton's jelly-derived mesenchymal stem cells (WJMSCs). To render the material hydrophilic and allow good material wettability, we treated the material either by plasma or by polydopamine (PDA) coating. We show that cells weakly adhere on these surfaces. Keeping the average pore diameter constant at 133 µm, we compare two structures, one with LARGE (52 µm) and one with SMALL (27 µm) interconnection diameters. DNA quantification and extracellular matrix (ECM) production reveal that larger interconnections is more suitable for cells to move across the scaffold and form a three-dimensional cellular network. We argue that LARGE interconnections favor cell communication between different pores, which then favors the production of the ECM. Moreover, PDA treatment shows a truly beneficial effect on fibroblast viability and on matrix production, whereas plasma treatment shows the same effect for WJMSCs. We, therefore, claim that both pore interconnection size and surface treatment play a significant role to improve the quality of integration of tissue engineering scaffolds.

Stable Freestanding Thin Films of Copolymer Melts Far from the Glass Transition.

Thin polymer films have attracted attention because of both their broad range of applications and of the fundamental questions they raise regarding the dynamic response of confined polymers. These films are unstable if the temperature is above their glass transition temperature Tg. Here, we describe freestanding thin films of centimetric dimensions made of a comb copolymer melt far from its glass transition that are stable for more than a day. These long lifetimes allowed us to characterize the drainage dynamics and the thickness profile of the films. Stratified regions appear as the film drains. We have evidence that the stability, thinning dynamics, and thickness profile of the films result from structural forces in the melt. Understanding the key mechanisms behind our observations may lead to new developments in polymeric thin films, foams, and emulsions without the use of stabilizing agents.

Packing spheres tightly: influence of mechanical stability on close-packed sphere structures.

Many experiments and simulations of packings of monodisperse hard spheres report a dominance of the face-centered cubic structure in the hexagonally close-packed limit, even though it has no significant energetic or entropic gain over other close-packed configurations. Combining simulations and experiments, we demonstrate that a simple mechanical instability which occurs during the packing process may play an important role in selecting the face-centered cubic structure over other close-packed alternatives. Our argument is supported by detailed quantitative analyses of key configurations in sphere packings and highlights the importance of the packing dynamics. The proposed mechanism is elementary and should therefore play a role in a wide range of sphere systems.

Structure and dynamics of confined foams: a review of recent progress.

Basic research on confined foams now points to an interesting application, a kind of microfluidics which deals with the manipulation of closely packed droplets or bubbles flowing in channels. In such systems, the minimisation of interfacial energy leads to self-organised ordering which is tightly coupled to the channel geometry, hence providing efficient means of performing controlled topological operations on droplet and bubbles structures. We have called this discrete microfluidics, and have begun to explore its possibilities and principles. Apart from the fact that such systems provide powerful tools to study the flow of foams and emulsions on the scale of a few bubbles or droplets, they also carry the promise of versatile applications for Lab-on-a-Chip technologies. In these, discrete gas or liquid samples can be generated, processed, stored and analysed within a single handheld chip. Previous work on foams and emulsions in confined geometries provides a basis for this, and is being extended progressively by new experiments and appropriate dynamic models, such as the 2d Viscous Froth Model. The result should be a practical "design kit" for more complex networks to efficiently process discrete gas and fluid samples.

The crystal structure of bubbles in the wet foam limit.

We have observed a rich variety of three-dimensional crystal and defect structures spontaneously formed by small (diameter 200 µm) bubbles in a wet foam. The observations confirm and extend those made by Bragg and Nye in 1947. However, while their experiments with two-dimensional bubble rafts have stimulated many researchers, their work on assemblages does not appear to have been followed up. These ordered packings now pose intriguing questions for the physics of foams. The bubbles seem too large for conventional thermodynamics and kinetics to easily explain the high degree of ordering.

The foam/emulsion analogy in structure and drainage.

The often quoted analogy between foams and emulsions is experimentally tested by studying properties after settling and under forced drainage of oil-in-water emulsions of drop size similar as for bubbles generally used in foam experiments. Observations with regard to structure, water fraction and drainage wave properties confirm the expected similarity in the low flow rate range. However, while for foams a convective circulation on the scale of the container sets in for values of water fraction exceeding about 0.2, no such convection is found in emulsions. Here instabilities are only encountered at water fractions of about 0.4, close to the void fraction of random packings of spheres. These take on the form of descending pulses of increased water fraction and lead to the transition from a frozen to a locally agitated structure.