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J Struct Biol ; 174(1): 11-22, 2011 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-21130884

RESUMO

The M protein of coronavirus plays a central role in virus assembly, turning cellular membranes into workshops where virus and host factors come together to make new virus particles. We investigated how M structure and organization is related to virus shape and size using cryo-electron microscopy, tomography and statistical analysis. We present evidence that suggests M can adopt two conformations and that membrane curvature is regulated by one M conformer. Elongated M protein is associated with rigidity, clusters of spikes and a relatively narrow range of membrane curvature. In contrast, compact M protein is associated with flexibility and low spike density. Analysis of several types of virus-like particles and virions revealed that S protein, N protein and genomic RNA each help to regulate virion size and variation, presumably through interactions with M. These findings provide insight into how M protein functions to promote virus assembly.


Assuntos
Coronavirus/metabolismo , Coronavirus/ultraestrutura , Proteínas da Matriz Viral/ultraestrutura , Montagem de Vírus/fisiologia , Montagem de Vírus/efeitos da radiação , Linhagem Celular , Proteínas M de Coronavírus , Microscopia Crioeletrônica , Tomografia com Microscopia Eletrônica , Humanos
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