An Experimental Study of the Effect of Diindolylmethane on Alveolocyte and Hepatocyte Adhesion Strength in Mice.

We studied the effect of diindolylmethane in a dose of 600 mg/kg on the change in adhesion strength of alveolocytes and hepatocytes in CBA mice. Diindolylmethane was administered intragastrically to experimental animals for 10 days, controls intragastrically received an equivalent volume of saline. At the end of the therapeutic period, mice treated with diindolylmethane showed a significant increase in the adhesion strength of alveolocytes by 16% (p=0.003) and hepatocytes by 61% (p=0.0001) in comparison with the control group, which indicates the antipromotor activity of diindolylmethane.

Preclinical antitumor activity of the diindolylmethane formulation in xenograft mouse model of prostate cancer.

AIM: Preclinical study of the specific anticancer pharmacological activity of the formulation containing active substance 3,3ʹ-diindolylmethane (DIM), cod liver oil, polysorbate 80 and α-tocopherol acetate (vitamin E), in vivo in a xenograft animal model of LNCaP. MATERIALS AND METHODS: The DIM, cod liver oil, polysorbate 80 and α-tocopherol acetate (vitamin E) formulation was intragastrically administered to BALB/c-nude (nu/nu) mice during 33 days post inoculation at the dose of 133 mg/kg/day. Antitumor activity of the test drug was estimated by the rate of tumor growth inhibition (T/C% - treated versus control), dividing the tumor volumes from treatment groups with the control groups. RESULTS: Statistically significant tumor xenograft regressions have been shown in group which received the DIM, cod liver oil, polysorbate 80 and α-tocopherol acetate (vitamin E) on the 37(th) day of observation post inoculation. The highest antitumor activity was achieved on the 39(th) day (T/C = 16,8%). Therapeutic effect lasts for 6 days after the end of therapy period. CONCLUSION: Our findings demonstrate inhibitory effect of the formulation on tumor development in the xenograft animal model due to the tumor growth rate reduction.

[An examination method for chemiluminescence of whole-blood leucocytes]. / Metod izucheniia khemoliuministsentsii leikotsitov tsel'noi krovi.

The chemiluminescence examination methods have been limited in use due to a lack of standard techniques for the preparation and storage of biological samples. The methods of sampling the blood and the conditions of its storage and transportation as well as other factors essentially affect the functional state of cells through activating them. The suggested technique keeps the values of blood chemiluminescence stable for 2 hours in measurements of spontaneous chemiluminescence and for 6 hours in measurements of stimulated chemiluminescence. For the purpose, whole blood is mixed, in test-tubes, with a 0.27% solution of disodium EDTA. It is stored in refrigerator at 0 to 5 degrees C. Blood is diluted by Henk solution and luminol for measurements.

[Effect of synthetic fragments of immunodominant regions of HIV viral proteins on the oxygen metabolism of human neutrophils]. / Vliianie sinteticheskikh fragmentov immunodominantnykh raionov belkov VICh na kislorodnyi metabolizm neitrofilov cheloveka.

Influence of synthetic peptides identical to fragments of natural human immunodeficiency virus (HIV) glycoproteins gp 120 and gp 41, on luminol-enhanced chemiluminescence (CL) of human neutrophils has been studied. It was established that some of peptide analogs of gp 120 and gp 41 immunodominant regions are able to suppress spontaneous CL: but when being used with dimethylsulfoxide they dramatically stimulate it and deteriorate opsonized zymosan-induced CL. Conclusions about the necessity of possible side effects considering during use of peptide vaccines against HIV have been made. It is also possible to explain some neutrophil dysfunction in HIV infected subjects as the result of HIV glycoproteins direct influence on this cells.