RESUMO
PREMISE OF THE STUDY: Microsatellite markers within regulators of heat stress transcription factors were identified in the Populus trichocarpa genome, and then developed for P. simonii to investigate the genetic diversity of germplasm resources and to further identify favorable alleles significantly associated with stress-resistant traits. METHODS AND RESULTS: Thirty-five novel microsatellite markers were identified from genes controlling heat stress transcription factors in P. simonii using a Sanger sequencing protocol. Polymorphisms in 48 individuals from 16 populations of P. simonii revealed that the number of alleles per locus ranged from two to nine with an average of 4.6; the observed heterozygosity and expected heterozygosity per locus varied from 0.143 to 0.857 and from 0.257 to 0.948, respectively. CONCLUSIONS: The new polymorphic markers developed during this study will facilitate the construction of genetic linkage maps and will aid in marker-assisted breeding of a new germplasm with desirable abiotic stress resistance in Populus species.
Assuntos
Proteínas de Ligação a DNA/genética , Proteínas de Choque Térmico/genética , Repetições de Microssatélites/genética , Proteínas de Plantas/genética , Populus/genética , Fatores de Transcrição/genética , Loci Gênicos/genética , Variação Genética , Fatores de Transcrição de Choque Térmico , Dados de Sequência MolecularRESUMO
PREMISE OF THE STUDY: Microsatellite markers from cellulose synthase genes were developed for the Chinese white poplar, Populus tomentosa, to investigate the genetic diversity of wild germplasm resources and to further identify favorable alleles significantly associated with wood cellulose content. METHODS AND RESULTS: Fifteen microsatellite markers were developed in P. tomentosa by deep sequencing of cellulose synthase genes. Polymorphisms were evaluated in 460 individuals from three climatic regions of P. tomentosa, and all 15 markers revealed polymorphic variation. The number of alleles per locus ranged from two to nine with an average of 4.3; the observed and expected heterozygosity per locus varied from 0.029 to 0.962 and from 0.051 to 0.713, respectively. CONCLUSIONS: These polymorphic markers will potentially be useful for genetic mapping and in molecular breeding for improvement of wood fiber traits in Populus.