RESUMO
An organocatalytic chemo- and regioselective C6-functionalization of 2,3-disubstituted indoles has been established via a reaction with ortho-hydroxybenzyl alcohols, which afforded biologically important diarylindol-6-ylmethanes in overall high yields (up to 99% yield). This protocol not only provides an efficient method for constructing biologically important diarylindol-6-ylmethane frameworks in an atom economical fashion, but also serves as a good example for the direct catalytic C6-functionalization of indoles, which have been rarely investigated. More importantly, the preliminary biological evaluation revealed that this new class of diarylindol-6-ylmethanes exhibited strong cytotoxicity to HeLa cell lines.
RESUMO
The signal communication between various organelles is essential for cells of eukaryotic organisms. Vesicle trafficking is an important pathway for this kind of communication. Most of the membrane fusion is mediated by SNAREs (Soluble N-ethyl-maleimide-sensitive fusion protein attachment protein receptors), which are highly conserved from various species. Compared with genomes of other eukaryotes, plant genome encodes an even higher number of SNAREs. Accumulating evidences support that plant SNAREs is a multifunctional protein family, which is involved in variety of biological processes. We review the recent advances on molecular mechanism and biological functions of plant SNAREs.
Assuntos
Transporte Biológico/fisiologia , Genoma de Planta/fisiologia , Imunidade Inata/fisiologia , Proteínas SNARE/fisiologia , Transdução de Sinais/genética , Sequência de Aminoácidos , Evolução Molecular , Previsões , Imunidade Inata/genética , Fusão de Membrana , Proteínas de Membrana/fisiologia , Modelos Biológicos , Dados de Sequência Molecular , Filogenia , Proteínas SNARE/genética , Alinhamento de Sequência , Homologia de Sequência de Aminoácidos , Transdução de Sinais/fisiologia , Proteínas de Transporte Vesicular/genéticaRESUMO
The effects of exogenous nitric oxide donor sodium nitroprusside (SNP) on substance metabolism of Ginkgo biloba leaves under drought stress were studied. The results showed that 250 micromol/L SNP (Fig.2) treatment under 35% relative soil water content (RSWC) stress (Fig.1) raised remarkably soluble sugar content (Fig.3), proline content (Fig.4), phenylalanine ammonia lyase (PAL) activity (Fig.5), flavonoids (Fig.6) and ginkgolides content (Fig.7) of G. biloba leaves. Hemoglobin, used as NO scavenger, counteracted the effects of SNP in raising the soluble sugar (Fig.3), proline (Fig.4), flavonoid (Fig.6), ginkgolide content (Fig.7) and PAL activities (Fig.5), which indicates that the effects of sodium nitroprusside were through the nitric oxide released from sodium nitroprusside. We propose from these results that the roles of flavonoids and ginkgolides are the same as those of soluble sugars and proline under drought stress. NO may alleviate the damage caused by drought stress through raising soluble sugar, proline, flavonoid and ginkgolide content.
