RESUMO
Safflopentsides A-C (1-3), three highly oxidized rearranged derivatives of quinochalcone C-glycosides, were isolated from the safflower yellow pigments. Their structures were determined based on a detailed spectroscopic analysis (UV, IR, HR-ESI-MS, 1D and 2D NMR), and the absolute configurations were confirmed by the comparison of experimental ECD spectra with calculated ECD spectra. Compounds 1-3 have an unprecedented cyclopentenone or cyclobutenolide ring A containing C-glucosyl group, respectively. The plausible biosynthetic pathways of compounds have been presented. At 10 µM, 2 showed strong inhibitory activity against rat cerebral cortical neurons damage induced by glutamate and oxygen sugar deprivation.
Assuntos
Carthamus tinctorius , Glicosídeos , Oxirredução , Glicosídeos/química , Glicosídeos/farmacologia , Glicosídeos/isolamento & purificação , Animais , Carthamus tinctorius/química , Ratos , Estrutura Molecular , Neurônios/efeitos dos fármacos , Relação Estrutura-Atividade , Fármacos Neuroprotetores/farmacologia , Fármacos Neuroprotetores/química , Fármacos Neuroprotetores/isolamento & purificação , Relação Dose-Resposta a Droga , Córtex Cerebral/efeitos dos fármacos , Chalconas/farmacologia , Chalconas/química , Chalconas/isolamento & purificaçãoRESUMO
Two new quinochalcone glycosides, hydroxysafflor yellow A-4'-O-ß-D-glucopyranoside (1) and 3'-hydroxyhydroxysafflor yellow A (2), were isolated from the safflower yellow pigments of Carthamus tinctorius. The structures of new compounds were elucidated by a detailed spectroscopic analysis (UV, IR, HR-ESI-MS, 1D and 2D NMR, ECD). The in vitro assay indicated that compound 1 could improve the survived rate of primary mouse cortical neurons on glutamate-induced neurons damage model at a concentration of 10 µM.