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Electron. j. biotechnol ; Electron. j. biotechnol;31: 75-83, Jan. 2018. tab, ilus, graf
Artigo em Inglês | LILACS | ID: biblio-1022130

RESUMO

Background: Phalaenopsis is an important ornamental flowering plant that belongs to the Orchidaceae family and is cultivated worldwide. Phalaenopsis has a long juvenile phase; therefore, it is important to understand the genetic elements regulating the transition from vegetative phase to reproductive phase. In this study, FLOWERING LOCUS T (FT) homologs in Phalaenopsis were cloned, and their effects on flowering were analyzed. Results: A total of five FT-like genes were identified in Phalaenopsis. Phylogenetic and expression analyses of these five FT-like genes indicated that some of these genes might participate in the regulation of flowering. A novel FT-like gene, PhFT-1, distantly related to previously reported FT genes in Arabidopsis and other dicot crops, was also found to be a positive regulator of flowering as heterologous expression of PhFT-1 in Arabidopsis causes an early flowering phenotype. Conclusions: Five FT homologous genes from Phalaenopsis orchid were identified, and PhFT-1 positively regulates flowering.


Assuntos
Proteínas de Plantas/genética , Arabidopsis , Orchidaceae/genética , Flores/genética , Reação em Cadeia da Polimerase/métodos , Clonagem Molecular , Genes de Plantas/genética , Biologia Computacional , Orchidaceae/crescimento & desenvolvimento , Flores/crescimento & desenvolvimento
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