Candidate gene association study for noise-induced hearing loss in two independent noise-exposed populations.

Millions of people are daily exposed to high levels of noise. Consequently, noise-induced hearing loss (NIHL) is one of the most important occupational health hazards worldwide. In this study, we performed an association study for NIHL based on a candidate gene approach. 644 Single Nucleotide Polymorphisms (SNPs) in 53 candidate genes were analyzed in two independent NIHL sample sets, a Swedish set and part of a Polish set. Eight SNPs with promising results were selected and analysed in the remaining part of the Polish samples. One SNP in PCDH15 (rs7095441), resulted in significant associations in both sample sets while two SNPs in MYH14 (rs667907 and rs588035), resulted in significant associations in the Polish sample set and significant interactions with noise exposure level in the Swedish sample set. Calculation of odds ratios revealed a significant association of rs588035 with NIHL in the Swedish high noise exposure level group. Our studies suggest that PCDH15 and MYH14 may be NIHL susceptibility genes, but further replication in independent sample sets is mandatory.

Noise-induced time-dependent changes in oxidative stress in the mouse cochlea and attenuation by D-methionine.

Oxidative stress in the cochlea is considered to play an important role in noise-induced hearing loss. This study determined changes in superoxide dismutase (SOD), catalase, lipid peroxidation (LPO) and the auditory brainstem response (ABR) in the cochlea of C57BL/6 mice prior to and immediately, 1, 3, 7, 10, 14 and 21 days after noise exposure (4 kHz octave band at the intensity of 110 dB SPL for 4 h). A significant increase in SOD activity immediately and on 1st day after noise exposure, without a concomitant increase in catalase activity suggested a difference in the time dependent changes in the scavenging enzymes, which facilitates the increase in LPO observed on day 7. The ABR indicated significant noise-induced functional deficits which stabilized in 2 weeks with a permanent threshold shift (PTS) of 15 dB at both 4 kHz and 8 kHz. The antioxidant D-methionine (D-Met) reversed the noise-induced changes in LPO levels and enzyme activities. It also significantly reduced the PTS observed on the 14th day from 15 dB to 5 dB for 4 kHz. In summary, the findings indicate that time-dependent alterations in scavenging enzymes facilitate the production of reactive oxygen species and that D-met effectively attenuates noise-induced oxidative stress and the associated functional loss in the mouse cochlea.

Hearing loss among workers exposed to moderate concentrations of solvents.

OBJECTIVES: It is known that some industrial organic solvents are ototoxic. This study was aimed at evaluating the hearing effects of a mixture of organic solvents alone or in combination with noise on employees in paint and lacquer enterprises. The concentration of solvents was below the occupational exposure limits (OEL) for most of the subjects. METHODS: Altogether 517 subjects were divided into the following three groups: persons with no risk due to noise or organic solvent exposure at the workplace, workers exposed to organic solvents only, and workers exposed to both organic solvents and noise. RESULTS: The relative risk (RR) of hearing loss in the solvent-only exposure group was significantly increased (RR 4.4 and RR 2.8 for noise exposure of < 80 dB-A and < 85 dB-A, respectively) in a wide range of frequencies (2-8 kHz). No additional risk in the solvent + noise exposure group was found (RR 2.8). Hearing thresholds were significantly poorer in a wide range of frequencies (1-8 kHz) for both groups exposed to solvents, when compared with the reference group. The mean hearing thresholds at frequencies of 2-4 kHz were poorer for workers exposed to solvents + noise than for the solvent-only group; this finding suggests an additional effect for noise. However, there was no correlation between hearing loss and the extent of solvent exposure. CONCLUSIONS: The results indicate that occupational organic solvent exposure at moderate concentrations increases the risk of hearing loss, and the ototoxic effects should be considered when the health effects of exposed workers are monitored.

[Assessment of low frequency noise annoyance in steering premises according to subjective annoyance rating by workers. A pilot study]. / Ocena uciazliwosci halasu niskoczestotliwosciowego w pomieszczeniach sterowniczych wg subiektywnej oceny pracowników--wyniki badan pilotazowych.

The study covered 192 workers aged 26-62 years (mean 41 +/- 6), employed at workposts in the control rooms. A questionnaire was applied as a main tool of the study. The aim of the study was subjective annoyance rating of low frequency noise at workplaces on a 100-degree rating scale. Using the same scale, the study subjects rated annoyance of reference wide-band noise (60 +/- 2, 70 +/- 2, 75 +/- 2 and 80 +/- 2 dB(A)) and reference low frequency noise (65 +/- 2, 70 +/- 2, 75 +/- 2 and 80 +/- 2 dB(A)) reproduced by phones. Questionnaire inquires were preceded by the measurements of A-, C- and G-weighted equivalent continuous sound pressure levels (LA eq.T, LC eq,T i LG eq,T). Noise with prominent low frequency components (10-250 Hz) at A-weighted sound pressure level of 47.7-65.2 dB was assessed as more than somewhat annoying (more than 25 degrees on the 100-degree annoyance rating scale) by 70% of workers examined. No correlation between subjective annoyance rating of the noise at the workplaces and LA eq,T or LG eq,T was noted. Statistically significant correlation (r = 0.23) between subjective annoyance rating and LC eq,T was observed. The subjective rating of annoyance of the reproduced low frequency noise and wide-band noise differed significantly. Low frequency noise was rated as more annoying than wide-band noise at the same A-weighted sound pressure levels.

[Hearing impairment in the plastics industry workers exposed to styrene and noise]. / Ocena uszkodzen sluchu u pracowników narazonych na styren i halas w przemysle tworzyw sztucznych.

Styrene exerts ototoxic effect in animals, and the combined exposure to this solvent and noise produces a synergistic effect relative to the isolated exposure to noise. However, there is a lack of reliable evidence that chronic occupational exposure to styrene is ototoxic to humans. Neither has been documented its additive effect with noise. The present study was aimed to assess hearing impairment and the risk of hearing loss in workers exposed to both styrene and noise. The study group included 72 male workers occupationally exposed to styrene in the plastics industry. The results were compared with two control groups, matched by age, gender and personal traits: one group composed of 82 male workers employed in a printing house and exposed only to noise at the levels almost twice as high as those observed in the group exposed to styrene and noise, and the other consisted of 65 male workers exposed neither to solvents nor to noise at workplace. In the group exposed to styrene and noise, significantly increased average hearing loss, as compared to both control groups, was found at all frequencies (1-8 kHz). The risk of hearing loss in the group exposed to styrene and noise was seven times higher (RR: 6.6; 3.0-15.9) than in the non-exposed group, and four times higher (RR: 4.0; 1.8-9.1) than in when workers exposed only to noise. However, no relationship could be observed between the amount of styrene exposure and hearing impairment, taking account of the confounding effect of noise and age. The results of the study suggest that the exposure to solvent mixtures with styrene as a basic component may exert additional, to noise, adverse effect on the auditory organ.

[Standardized new method for measuring vibration perception]. / Standaryzacja nowej metodyki badania czucia wibracji.

The examination of the vibration perception is one of the basic measurements applied in the evaluation of the peripheral neuropathies in hand-arm vibration syndrome (HAVS) The detailed guidelines on how this kind of examinations should be performed, presented in the 1998 ISO standard, differ substantially from those currently binding in Poland. The aim of this study was to standardize the method of vibration perception measurements, taking account of the parameters consistent with the ISO recommendations, such as algorithm (stimulus presentation method), vibration frequency, the size and contact force of vibrating probe, as well as the comparison of the results obtained by means of both methods (ISO-recommended method and standard Polish method). It was found that the algorithm change (ascending method replaced by Bekesy's technique, recommended by ISO) did not affect the of the vibration perception thresholds values within the wide range of frequencies (32-500 Hz), whereas the decreased size of probe, pressed with smaller contact force, resulted in significantly higher values of perception thresholds within the frequency range of 125-400 Hz. The comparison of the outcomes of the ISO-recommended and Polish standard methods revealed that the perception thresholds did not differ at the frequency of 125 Hz, but at the frequencies of 250 and 400 Hz, the ISO-recommended method produced higher values. Moreover, at the frequencies ranging from 125 to 400 Hz, the results of the perception threshold measurements, taken several times in the same persons (intrasubject variability) by means of both methods, were more scattered. It was revealed that the measurements at the frequency range of 4-125 Hz should be included in the vibration perception examinations, and the certification of the ability to work in the conditions of exposure to hand-arm vibration should be based on their results. It is also essential that in the standard examination, a smaller vibratory probe (phi = 5 mm) and lighter contact force (0.1 N) than those currently used, should be applied.

[Assessment of hearing impairment in workers exposed to mixtures of organic solvents in the paint and lacquer industry]. / Ocena uszkodzen sluchu u pracowników narazonych na mieszaniny rozpuszczalników organicznych w przemysle farb i lakierów.

Clinical and experimental studies indicate a possible harmful effect of chemicals, especially organic solvents, on the hearing system. In combined exposure to noise and solvents, very common in industry, it is most likely that a synergetic action of these factors enhances the traumatising effect of exposure to noise. The aim of this study was to assess the incidence and the risk of hearing impairment in 117 paint and lacquer factory workers exposed to a mixture of organic solvents. An analysis of organic solvent mixtures reveals that xylene and ethyl acetate are their major components whose concentrations depend on individual workposts. The control group consisted of 76 workers exposed to noise exceeding, Threshold Limit Value and 125 healthy subjects exposed neither to noise nor to solvents in their occupational setting. Pure tone audiometry revealed the highest hearing thresholds in workers exposed to solvents, lower thresholds in those exposed to noise, and the lowest ones in the non-exposed individuals. Hearing loss was found in 30% of workers exposed to organic solvents, in 20% of noise-exposed subjects, and in only 6% of non-exposed subjects. The comparison of relative risk values also indicated significantly enhanced probability of hearing impairment in workers of the paint and lacquer factory (9.6; 3.2-25.6), which is even more strongly pronounced than in the group of subjects exposed to noise (4.2; 1.2-13.2). An analysis of hearing impairment risk in particular frequencies suggests that organic solvents may damage the inner ear in much greater extent than noise. The results of the study show that exposure to organic solvents may create a significant risk of hearing impairment. Therefore, further steps should be taken to include the exposed population into effective preventive programmes.