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Despite a high volume of market (cull) dairy cows entering the food chain every year, beef from market dairy cows is largely considered insignificant when compared with beef from beef cattle in the market and is widely thought to be used for ground beef only. Our study aimed to evaluate the effects of feeding dairy market cows on a lactating TMR diet before slaughter on carcass and beef quality traits. Forty-three Holstein market cows were randomly assigned into 2 treatments: Fed or Direct. Fed cows (n = 22) were dried off, then fed a lactating cow TMR ration for approximately 60 d whereas Direct cows (n = 21) were sent directly to slaughter. Hot carcass weight (HCW) was used to calculate the dressing percentage (DP) together with the animals' body weight recorded when animals left the farm to go to the abattoir. At 24h post-mortem, the rib fat thickness (RFT) and rib eye area (REA) were measured by a certified grader. Afterward, rib samples were collected between the 12th -10th ribs from one side of the carcass and divided into 5 ribeye steaks. The first steak was used for intramuscular fat (IMF) content analysis by NIRS. The other 4 steaks were then aged for 7, 14, 21, or 28 d. Following aging, the steaks available were assessed for tenderness using the Warner-Bratzler Shear Force protocol. Data were analyzed by mixed linear regression to compare experimental groups for continuous outcomes. HCW of the Fed cows was higher than the Direct cows, with a mean HCW of 408.3 kg and 326.1 kg, respectively (SE ± 9.0). Average DP in Fed cows was 49.1%, compared with 42.5% in Direct cows (SE ± 0.69). The average percentage of IMF in Fed cows was 8.1% and in Direct cows was 5.0% (SE ± 0.43). Fed cows had a mean REA of 68.1 cm2 and Direct cows had a mean of 57.7 cm2 (SE ± 11.9). No differences were found for RFT. The steaks aged for 14 d resulted in meat with a mean shear force (SF) of 4.19 kg in Fed cows, while Direct cows had a mean SF of 6.28 kg (SE ± 0.29) Feeding market dairy cows for 60 d before slaughter enhanced carcass weight and yield, IMF content, and tenderness. These results indicate that approximately 60 d of feeding can improve the quality of market dairy cow meat and may help improve market cow value.
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The objective of this study was to quantify the effects of supplementing early-lactation cows with a dry pure glycerol product, delivered through the automated milking system (AMS) concentrate, in the first 21 d in milk (DIM) on metabolic markers, milking behavior, and milk production. In 5 commercial AMS dairy herds, 389 dairy cows were randomly assigned, controlling for parity, 21 d before expected calving to 1 of 2 treatments, within farm: (1) control group (CON) receiving the standard AMS pellet (n = 213) from 1 to 150 DIM, or (2) glycerol group (GLY) receiving the treatment AMS pellet (n = 176) formulated to deliver 250 as fed g/d of glycerol product from 1 to 21 DIM (mean actual = 249 g/d dry matter [DM]), then they received the standard AMS pellet from 22 to 150 DIM. Across all farms, cows were fed partial mixed rations (PMR) that were similar in ingredient and nutrient composition. One prepartum blood sample and 5 postpartum blood samples were collected from each cow to determine serum nonesterified fatty acids (NEFA), blood ß-hydroxy butyrate (BHB), and blood glucose concentrations. Cow body condition score (BCS) was recorded every 21 d from -21 to 63 DIM. Data were collected and analyzed for the treatment period (1 to 21 DIM) and a follow-up period (22 to 150 DIM). There was no detected treatment effect on serum NEFA concentrations in the first week of lactation. There was a treatment by time interaction for blood BHB and blood glucose, where GLY cows tended to have increased BHB concentrations at 5 DIM and had decreased glucose concentrations at 9 and 12 DIM. There was an interaction of BCS with treatment on the incidence of BHB ≥1.2 mmol/L, whereby over-conditioned CON cows (BCS ≥3.5) were 3.5x more likely to have a high BHB test than CON cows with normal prepartum BCS. During the treatment period GLY cows had 0.1 ± 0.05 more successful milkings/d, were delivered 0.27 ± 0.05 DM kg/d more AMS concentrate and tended to yield 0.8 ± 0.47 kg/d more milk. During the follow-up period GLY cows had 0.1 ± 0.04 more successful milkings/d, were delivered 0.18 ± 0.06 DM kg/d more AMS concentrate, and yielded 1.5 ± 0.53 kg/d more milk than CON cows. Glycerol supplementation allowed cows to maintain better BCS, as GLY cows lost less BCS from calving to 63 DIM than CON cows. Overall, the results of this study demonstrate that supplementing pure glycerol through the AMS concentrate for the first 21 DIM can reduce BCS loss in early lactation, improve milking behavior, and increase milk yield to mid lactation.
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The welfare of cull cows during transport to slaughter is a current concern in the Canadian dairy industry. Cull cows sold through auction often have a high prevalence of lameness, low BCS, hock lesions, and udder engorgement. To evaluate whether drying off and feeding cull dairy cows before transport can mitigate these challenges, 45 cows designated for culling were randomly assigned to either be fed for 60 d after being dried off (fed group; n = 24) or to serve as controls by being sent directly to slaughter (direct group; n = 21). Two fed group cows were removed for health reasons before completing the feeding period. Both fed group and direct group cows were assessed for locomotion (5-point scale), BCS (5-point scale), hock lesions (3-point scale), udder engorgement (3-point scale), and BW at the time of enrollment. Fed group cows, locomotion, BCS, hock, and udder engorgement scores were assessed weekly until slaughter. Weights of the fed group cows were measured again the day before slaughter. Mixed linear regression models were used to assess continuous outcomes BCS and weight. Mixed logistic regression models were used to assess dichotomous outcomes presence of hock lesions and lameness. Fed group cows gained an average of 116.9 kg over the feeding period (SE ± 8.20). Fed group cows had an average weight at slaughter of 834.2 kg, whereas direct group cows' average weight was 767.3 kg (SE ± 26.8). The fed group cows' average BCS at the start of the trial was 2.4, and at slaughter was 3.6, with an average gain of 1.2 BCS points. At slaughter, the proportion of udders involuted in the fed group was 45.1% (10/22) and in the direct group cows, was 0% (0/21). No differences were found in locomotion or hock lesions between the fed group and direct groups. It is important to weigh potential benefits for the fed group cows with the fact that direct group cows did not endure a drying off procedure, nor were they placed at risk of potential adverse health events. However, despite these potential limitations, due to the improved BCS and udder engorgement scores, cows fed for 60 d may be better prepared for the transportation to slaughter, as well as sell for a higher price due to increased BW and body condition.
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Coxeadura Animal , Animais , Bovinos , Feminino , Doenças dos Bovinos , Glândulas Mamárias Animais , Indústria de Laticínios , Meios de TransporteRESUMO
The objectives of this study were to determine the farm-level hyperketolactia (HKL) prevalence, as diagnosed from milk ß-hydroxybutyrate (BHB) concentration, on dairy farms milking with an automatic milking system (AMS) and to describe the farm-level housing, management, and nutritional risk factors associated with increased farm-average milk BHB and the within-herd HKL prevalence in the first 45 DIM. Canadian AMS farms (n = 162; eastern Canada n = 8, Quebec n = 23, Ontario n = 75, western Canada n = 55) were visited once between April to September 2019 to record housing and herd management practices. The first test milk data for each cow under 45 DIM were collected, along with the final test of the previous lactations for all multiparous cows, from April 1, 2019 to September 30, 2020. The first test milk BHB was then used to classify each individual cow as having HKL (milk BHB ≥ 0.15 mmol/L) at the time of testing. Milk fat and protein content, milk BHB, and HKL prevalence were summarized by farm and lactation group (all, primiparous, and multiparous). During this same time period, formulated diets for dry and lactating cows, including ingredients and nutrient composition, and AMS milking data were collected. Data from the AMS were used to determine milking behaviors and milk production of each herd during the first 45 DIM. Multivariable regression models were used to associate herd-level housing, feeding management practices, and formulated nutrient composition with first test milk BHB concentrations and within-herd HKL levels separately for primiparous and multiparous cows. The within-herd HKL prevalence for all cows was 21.8%, with primiparous cows having a lower mean prevalence (12.2 ± 9.2%) than multiparous cows (26.6 ± 11.3%). Milk BHB concentration (0.095 ± 0.018 mmol/L) and HKL prevalence for primiparous cows were positively associated with formulated prepartum DMI and forage content of the dry cow diet while being negatively associated with formulated postpartum DMI, the major ingredient in the concentrate supplemented through the AMS, and the PMR-to-AMS concentrate ratio. However, multiparous cows' milk BHB concentration (0.12 ± 0.023 mmol/L) and HKL prevalence were positively associated with the length of the previous lactation, milk BHB at dry off, prepartum diet nonfiber carbohydrate content, and the major forage fed on farm, while tending to be negatively associated with feed bunk space during lactation. This is the first study to determine the farm-level risk factors associated with herd-level prevalence of HKL in AMS dairy herds, thus helping optimize management and guide diet formulation to promote the reduction of HKL prevalence.
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The objective of this study was to describe the nutritional strategies used on Canadian dairy farms with automated milking systems (AMS), both at the feed bunk and the concentrate offered at the AMS, as well as to determine what dietary components and nutrients, as formulated, were associated with milk production and milking behaviors on those farms. Formulated diets (including ingredients and nutrient content) and AMS data were collected from April 1, 2019, until September 30, 2020, on 160 AMS farms (eastern Canada [East] = 8, Ontario [ON] = 76, Quebec [QC] = 22, and western Canada [West] = 54). Both partial mixed ration (PMR) and AMS concentrate samples were collected from May 1 to September 30, 2019, on 169 farms (East = 12, ON = 63, QC = 42, West = 52). We collected AMS milking data for 154 herds. For each farm (n = 161), milk recording data were collected and summarized by farm to calculate average milk yield and components. Multivariable regression models were used to associate herd-level formulated nutrient composition and feeding management practices with milk production and milking behavior. Milk yield (mean ± SD = 37.0 ± 0.3 kg/d) was positively associated with the PMR ether extract (EE) concentration (+0.97 kg/d per percentage point [p.p.] increase) and with farms that fed barley silage as their major forage source (n = 16; +2.18 kg/d) as compared with haylage (n = 42), whereas farms that fed corn silage (n = 96; +1.23 kg/d) tended to produce more milk than farms that fed haylage. Greater milk fat content (4.09 ± 0.28%) was associated with a greater PMR-to-AMS concentrate ratio (+0.02 p.p. per unit increase) and total diet net energy for lactation (+0.046 p.p. per 0.1 Mcal/kg increase), but a lesser percentage of NFC of the PMR (-0.016 p.p. per p.p. increase of NFC percentage). Milk protein content (3.38 ± 0.14%) was positively associated with the forage percentage of the PMR (+0.003 p.p. per p.p. increase of forage percentage) and the total diet starch percentage (+0.009 p.p. per p.p. increase of starch percentage), but was negatively associated with farms feeding corn silage (-0.1 p.p. compared with haylage) as their major forage. Greater milking frequency (2.77 ± 0.40 milkings/d) was observed on farms with free-flow cow traffic systems (+0.62 milkings/d) and was positively associated with feed push-up frequency (+0.013 milkings/d per additional feed push-up), but negatively associated with PMR NFC content and forage percentage of the total ration (-0.017 milkings/d per p.p. increase of forage percentage). Lastly, greater milking refusal frequency (1.49 ± 0.82 refusals/d) was observed on farms with free-flow cow traffic systems (+0.84 refusals/d) and farms feeding barley silage (+0.58 refusals/d) than with guided flow and farms feeding either corn silage or haylage, respectively. These data give insight into the ingredients, nutrient formulations and type of diets fed on AMS dairy farms across Canada and the association of those factors with milk production and milking behaviors.
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Indústria de Laticínios , Dieta , Lactação , Leite , Animais , Bovinos , Feminino , Leite/química , Dieta/veterinária , Canadá , Fazendas , Ração Animal/análiseRESUMO
The objective of this study was to quantify the effects of supplementing a low level of dry glycerol product pre- and postpartum on the feeding behavior, lying behavior, and reticulorumen pH of dairy cows. Multiparous Holstein dairy cows (n = 60) were enrolled in a 2 × 2 factorial design study. Twenty-one days before expected parturition, cows individually received a dry cow diet with (1) 250 g/d glycerol supplementation (GLY; 66% pure glycerol, United States Pharmacopeia grade), or (2) no supplementation (CON). Following parturition, cows were individually assigned to either (1) 250 g/d glycerol product (GLY; 66% pure glycerol), or (2) no supplementation (CON) to their partial mixed ration (PMR) for the first 21 d in milk (DIM). All cows were milked by an automated milking system and offered a target of 5.4 kg/d pellet (23% of target total dry matter intake [DMI]). For both treatment periods, cows were individually assigned to automated feed bins to measure PMR feeding behavior. Rumination time and lying behavior were monitored with electronic sensors for the whole study (-21 to 21 DIM). Reticulorumen pH boluses were administered to a subset of cows (n = 40) where pH was recorded every 10 min from 21 d prepartum to 21 d postpartum. Prepartum, cows fed GLY had fewer, larger meals and spent 20.2% more time feeding than CON while consuming feed at a similar rate. Cows on the CON diet prepartum spent more time lying down in more frequent bouts in the 21 d before calving. Following parturition, cows that received GLY prepartum continued to devote more time to eating, while tending to spend less time ruminating per kilogram of DMI. Cows receiving CON postpartum had larger meals with longer intervals between meals. In the first 21 DIM, cows receiving CON prepartum tended to have shorter, but significantly more frequent, lying bouts than cows fed GLY prepartum. Glycerol supplementation pre- and postpartum resulted in less time spent lying down following parturition. Minimal differences between treatments were observed for pre- and postpartum sorting behavior or reticulorumen pH. Overall, supplementation of glycerol pre- and postpartum altered cow time budgets, with cows spending more time eating pre- and postpartum, less time lying pre- and postpartum, and having fewer, larger meals prepartum when receiving glycerol prepartum, and with cows having slower feeding rates and smaller meals following parturition with postpartum glycerol supplementation.
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Glicerol , Lactação , Feminino , Bovinos , Animais , Período Pós-Parto , Suplementos Nutricionais , Comportamento Alimentar , Concentração de Íons de HidrogênioRESUMO
The objective of this study was to quantify the effects of supplementing transition dairy cows with a low inclusion dry glycerol product in the pre- and postpartum periods on feed intake, metabolic markers, and milk yield and components. Multiparous Holstein dairy cows (n = 60) were enrolled in a 2-by-2 factorial design study. Starting 21 d before expected parturition, cows individually received a dry cow diet with (1) 250 g/d glycerol product supplementation [66% pure glycerol (United States Pharmacopeia grade); GLY], or (2) no supplementation (CON) mixed to their total mixed ration. After parturition, cows, again, were individually assigned to either GLY, or (2) no supplementation (CON) to their partial mixed ration for the first 21 d in milk (DIM). Cows were milked by an automated milking system and offered a target of 5.4 kg DM/d pellet (23% of target total dry matter intake, DMI) in the automated milking system and followed for 42 d into lactation. Blood samples were collected 6.3 ± 3.47 d before calving for all blood measures and 3, 7, 10, and 14 DIM for analysis of glucose and ß-hydroxybutyrate, as well as 3 and 7 DIM for nonesterified fatty acids (NEFA) and haptoglobin. Initial dry cow body weight (BW), calf birth weight, previous 305-d milk, and month of parturition were used as covariates in the statistical model. Cows supplemented with GLY prepartum lost less BW and consumed more DMI pre- and postpartum, as well as had lower postpartum blood ß-hydroxybutyrate and NEFA concentrations compared with those fed the CON treatment prepartum. Cows supplemented with GLY postpartum had lesser DMI in the first 42 DIM than cows fed CON postpartum, but also had reduced blood NEFA concentrations, odds of a high haptoglobin test, odds of a low blood glucose test, and lesser preformed fatty acid concentrations and yields in their milk. Cows supplemented glycerol both pre- and postpartum lost the least total BW from -21 to 21 DIM. No treatment effects were detected for milk yield; however, cows receiving GLY postpartum had lower milk fat. Overall, glycerol supplementation during the transition period, particularly during the 21 d before calving, was associated with markers of improved metabolic status.
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Glicerol , Leite , Gravidez , Feminino , Bovinos , Animais , Leite/metabolismo , Glicerol/metabolismo , Ácidos Graxos não Esterificados , Ácido 3-Hidroxibutírico , Haptoglobinas/metabolismo , Período Pós-Parto , Lactação/metabolismo , Dieta/veterinária , Ingestão de Alimentos , Suplementos NutricionaisRESUMO
The objectives of this study were (1) to describe navel healing in male and female neonatal Holstein calves over a 14-d observation period, and (2) to determine the associations of dam parity, calving factors, and calf factors with navel measurements and time to healing. A total of 68 Holstein calves (43 female and 25 male) were enrolled. Navel healing outcomes (cessation of navel discharge, navel cord remnant drying, navel cord remnant drop-off) and navel stump measurement outcomes (proximal diameter, distal diameter, length) were recorded every 24 ± 1 h for a 14-d observation period. Data were also collected for dam parity, calving factors (time of birth, calving ease), and calf factors (calf sex, calf vitality at birth, birth weight, average daily gain, serum total protein, and medical treatment or treatments). The mean time to navel cord remnant drying was 2 d, and all calves had dry navels by d 5. A total of 56% of calves retained their navel cord remnant beyond the end of the observation period, and calves began to shed their navel cord remnants as early as d 2. In addition, at least once during the observation period, 85% of calves had a proximal navel diameter measurement over a 13-mm threshold recently proposed to identify navel infections. For distal navel diameter measurements, 72% of calves had a diameter over the 13-mm threshold at least once during the observation period. No associations were identified between navel healing outcomes and dam, calving, and calf factors. Navel measurement outcomes were associated with calving and calf factors. Specifically, proximal navel stump diameter was associated with the day of observation (decreased from d 5 to 14), calf sex (smaller in female calves), time of birth (larger in calves born overnight or unobserved, compared with the morning), and birth weight (larger in birth weights >44.1 kg compared with <38.5 kg). Distal navel stump diameter was associated with calf sex, medical treatment, and day of observation; however, we observed an interaction between sex and day of observation (larger in male calves on d 3 to 7, d 9, and d 12, compared with female calves), and sex and medical treatment (larger in untreated male calves, no difference between treated male and female calves). Navel stump length was associated with time of birth (longer in calves born overnight or unobserved compared with any other time) and the interaction of birth weight and day of observation (longer in birth weights >44.1 kg compared with 38.6-41.5 kg on d 3, 8-10, and 13, and 41.6-44.0 kg on d 3, 5, 7-8, and 13). The wide range in time to navel cord remnant drying and drop-off suggest that these measures may not be adequate indicators of calf age on their own. More research on navel healing is required to improve our understanding of factors that influence navel measurements and time to healing, as well as how this information could be used in an objective assessment of navel health.
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Ingestão de Alimentos , Animais , Animais Recém-Nascidos , Peso ao Nascer , Bovinos , Estudos de Coortes , Feminino , Humanos , Estudos Longitudinais , Masculino , GravidezRESUMO
Ketosis in dairy cattle is primarily diagnosed based on the concentrations of ketone bodies in the blood, milk, or urine. Cow-side tests using these fluids are available for rapid detection of elevated concentrations of ketone bodies. Although these tests have been extensively validated, the performance of different tests has not been compared over time. Our objectives were to investigate the relationship between point-of-care diagnostic tests measuring the concentrations of ß-hydroxybutyrate (BHB) in blood (BT; Precision Xtra, Abbott Laboratories), BHB in milk (MT; Keto-Test, Elanco), and acetoacetate (AcAc) in urine (UT; Ketostix, Bayer Corporation) through cases of ketosis. Holstein cows (n = 148) were screened daily for hyperketonemia (HYK; blood BHB ≥1.2 mmol/L) from 3 to 16 d in milk (DIM); moreover, milk and urine samples were collected concomitantly and tested for ketones (ketosis thresholds: 100 µmol/L milk BHB and 5 mg/dL urine AcAc). Of the animals screened (n = 148), 74% were diagnosed with HYK. When diagnosed with HYK, cows were treated with propylene glycol orally once daily for 5 d. After the day of diagnosis (d 0), hyperketonemic cows were retested with BT, MT, and UT for 3 d (d 1, 2, and 3). We assessed the diagnostic test performance and time to ketosis (survival analyses and Cox proportional hazards models) of MT and UT compared with BT. Considering all paired samples (before and after diagnosis of HYK), MT had 61% sensitivity and 91% specificity, whereas the UT had 77% sensitivity and 94% specificity compared with BT. The specificity of MT and UT increased from d 0 to d 1, decreased on d 2, and increased on d 3. The median time to diagnosis of ketosis in blood was 5 DIM (95% CI 5 to 7 DIM); moreover, MT and UT had 2 d greater median time to diagnosis of ketosis compared with the BT [7 DIM (6 to 11 d); and 7 DIM (6 to 13 d), respectively]. We concluded that the UT is a more sensitive predictor of blood BHB concentration than the MT. The UT and MT tests diagnosed ketotic cows approximately 2 d later than the BT. The possible consequences of delay in detection of ketosis in milk and urine should be investigated.
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Doenças dos Bovinos , Cetose , Ácido 3-Hidroxibutírico , Animais , Bovinos , Doenças dos Bovinos/diagnóstico , Feminino , Corpos Cetônicos , Cetose/diagnóstico , Cetose/veterinária , Lactação , LeiteRESUMO
Impaired locomotion (lameness) may negatively affect the ability and desire of cows to milk voluntarily, which is a key factor in success of automated milking systems (AMS). The objective of this study was to identify factors associated with herd-level lameness prevalence and associations of lameness and other farm-level factors with milking activity, milk yield, and milk quality in herds with AMS. From April to September 2019, 75 herds with AMS in Ontario, Canada, were visited, and data on barn design and farm management practices were collected. Data from AMS were collected, along with milk recording data, for the 6-mo period before farm visits. Farms averaged 98 ± 71 lactating cows, 2.3 ± 1.5 robot units/farm, 43.6 ± 9.4 cows/robot, 36.4 ± 4.9 kg/d of milk, a milking frequency of 3.01 ± 0.33 milkings/d, and a herd average geometric mean SCC of 179.3 ± 74.6 (× 1,000) cells/mL. Thirty percent of cows/farm (minimum of 30 cows/farm) were scored for body condition (1 = underconditioned to 5 = over conditioned) and locomotion (1 = sound to 5 = lame; clinically lame ≥3 out of 5 = 28.3 ± 11.7%, and severely lame ≥4 out of 5 = 3.0 ± 3.2%). Clinical lameness (locomotion score ≥3) was less prevalent on farms with sand bedding, with increased feed bunk space per cow, and on farms with non-Holstein breeds versus Holsteins, and tended to be less prevalent with lesser proportion of underconditioned cows (with body condition score ≤2.5). Severe lameness occurrence (farms with any cows with locomotion score ≥4) was associated with a greater proportion of underconditioned cows and in farms with stalls with greater curb heights. Herd average milk yield/cow per day increased with lesser prevalence of clinical lameness (each 10-percentage-point decrease in clinical lameness prevalence was associated with 2.0 kg/cow per day greater milk yield) and greater milking visit frequency per day, and tended to be greater with increased feed push-up frequency. Lesser herd average somatic cell count was associated with lesser clinical lameness prevalence, herd average days in milk, and proportion of overconditioned cows, and somatic cell count tended to be lesser for farms with sand bedding versus those with organic bedding substrates. The results highlight the importance of minimizing lameness prevalence, using of sand bedding, ensuring adequate feed access and feed bunk space, and maintaining proper cow body condition to optimize herd-level productivity and milk quality in AMS herds.
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Doenças dos Bovinos , Leite , Animais , Bovinos , Doenças dos Bovinos/epidemiologia , Indústria de Laticínios , Fazendas , Feminino , Abrigo para Animais , Lactação , Coxeadura Animal/epidemiologia , Ontário , PrevalênciaRESUMO
Nonambulatory dairy cattle pose a complex problem due to the challenges associated with prevention, appropriate treatment and management, and arriving at an accurate prognosis. There is a breadth of literature regarding this topic, of which there is currently no formal synthesis. The objective of this scoping review was to describe and characterize the literature investigating risk factors, sequela, preventions, treatments, and prognostic factors for nonambulatory conditions in dairy cattle, with the intent of qualitatively synthesizing knowledge of the topic and identifying gaps in the literature. A literature search was conducted in 6 databases and 2 conference proceeding archives, which returned 7,568 unique articles. Initial screening of abstracts resulted in 1,544 articles reviewed at the full-text stage, of which 379 were included for data extraction. Over 75% of the included literature was published after 1980, and the most common countries in which these studies took place were the United States (n = 72), Canada (18), Sweden (17), and Germany (17). Common eligibility criteria used for inclusion were geographic region (97) and parity (92). Of the 379 studies included in this review, 144 were randomized controlled trials and 235 were observational studies. The majority of the controlled trials assessed prevention of nonambulatory conditions (116), most commonly through supplementation of vitamin D (27) and calcium (25) or the provision of anionic salts (22). Of the 28 studies focusing on treatment of nonambulatory conditions, 26 focused on calcium administration. Becoming nonambulatory was evaluated as an outcome in 165 of the observational studies. Frequently measured risk factors for becoming nonambulatory included hematological variables, such as blood calcium (73), phosphorus (53) and magnesium (42), and other factors such as parity (35) and breed (22). Recovery from a nonambulatory condition was the outcome in 31 of the observational studies, with commonly measured prognostic indicators being calcium (9), phosphorus (9), and duration of recumbency (7). Nonambulatory disorders were measured as risk factors in 53 of the observational studies, with the most commonly assessed outcomes including disorders of the transition period (11), and death or euthanasia (11). The most common terms used to describe nonambulatory conditions were "milk fever" (199) and "parturient paresis" (147). These terms were only further defined with explicit symptomatic criteria in 193 of the 379 studies in this review. Recumbency was the most commonly used of these criteria (144), followed by inability to rise (55). Potential gaps in the literature concerning nonambulatory dairy cattle that were identified in the present review included investigation of prognostic indicators for recovery from nonambulatory conditions that are applicable on farm, treatment alternatives to calcium administration, and guidance regarding the appropriate usage of terms meant to categorize nonambulatory dairy cattle.
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Doenças dos Bovinos , Hipocalcemia , Paresia Puerperal , Animais , Bovinos , Doenças dos Bovinos/prevenção & controle , Feminino , Hipocalcemia/veterinária , Magnésio , Paridade , Paresia Puerperal/prevenção & controle , GravidezRESUMO
Understanding how downer cattle are managed allows for the evaluation of strengths and weaknesses in these practices, which is an important step toward improving the care these animals receive. The objective of this cross-sectional study was to analyze factors associated with the care and management of downer cattle by Canadian dairy producers. Data were obtained from the 2015 National Dairy Study, and analysis was limited to the 371 respondents completing the downer cow scenario. The scenario described a downer cow that the producer wanted to keep in their herd but must be moved, and was followed by questions addressing the cow's care and management. Using multivariable logistic regression models, associations between respondent demographics and farm characteristics, and the presence of downer cow protocols, we assessed decisions regarding euthanasia and use of behavioral prognostic indicators. Written downer cow protocols were reported by 18.2% of respondents, 67% indicated that they had a nonwritten protocol, and 14.8% reported that they did not have a protocol (either written or nonwritten). Respondents from western provinces were more likely to have a written protocol than those from Ontario. Nineteen percent of the respondents with a written or unwritten protocol reported veterinary involvement in developing their downer cow protocol, which occurred more commonly on farms with more frequent herd health visits and a good producer-veterinarian relationship. An area to move a downer cow to was present on 88% of farms, with respondents who were farm staff being less likely to report having knowledge of a designated area than respondents who were the farm owner. In addition, approximately half (45%) of respondents reported moving downer cattle with hip lifters as their most common method. Behavioral prognostic indicators chosen by respondents were associated with the respondent's geographic region, age, farm size, and education. Most notably, older respondents were more likely to use appetite, and less likely to use attitude, as a prognostic indicator compared with younger respondents. Using perceived pain as a prognostic indicator was more common among respondents from western and Atlantic provinces compared with respondents from Ontario, and more common among respondents with a college or university education. These results highlighted herd and farmer demographics that were associated with how Canadian dairy producers managed downer cattle in 2015 and could be used as a benchmark for evaluating how these management practices compare with those currently implemented.
Assuntos
Doenças dos Bovinos , Indústria de Laticínios , Animais , Bovinos , Estudos Transversais , Eutanásia Animal , Fazendas , Feminino , OntárioRESUMO
This randomized controlled trial investigated the effects of temporarily reducing milking frequency (MF) on the resolution of ketosis and milk production in dairy cows in early lactation. To detect ketosis [blood ß-hydroxybutyrate (BHB) ≥1.2 mmol/L], Holstein cows were screened daily from 3 to 16 d in milk using a cow-side meter. Cows diagnosed with ketosis (n = 104) were randomly assigned to twice-daily milking (TDM) or reduced to once-daily milking (ODM) for 2 wk, then returned to twice-daily milking. Both treatment groups received a 5-d treatment of an oral propylene glycol drench (PG; 300 g) beginning on the afternoon of the diagnosis; cows received additional 5-d PG treatments if they had a ketotic test result (blood BHB ≥1.2 mmol/L) at least 4 d after finishing the first PG treatment. Blood BHB tests were conducted for the first 3 d after ketosis diagnosis, and then once every 3 d for 21 d of trial (DOT). Milk and milk component data were collected weekly for 15 wk following trial enrollment. The ODM group showed rapidly and markedly decreased blood BHB concentrations (primiparous cows: 1 DOT, 0.92 ODM vs. 1.22 TDM, 15 DOT, 0.55 vs. 0.81 mmol/L; multiparous cows: 1 DOT, 1.01 vs. 1.40, 15 DOT, 0.78 vs. 1.65 mmol/L). In addition, a logistic regression model indicated that ODM cows were less likely to have blood BHB concentrations ≥1.2 mmol/L [primiparous cows: 3 DOT: ODM 1% (95% confidence interval: 0-10%) vs. TDM 43% (30-58%), 15 DOT ODM 0% (0-0.2%) vs. TDM 22% (13-36%); multiparous cows: 3 DOT: ODM 33% (24-44%) vs. TDM 59% (48-69%), 15 DOT ODM 20.9% (13-31%) vs. TDM 64% (53-74%)]. The proportion of ODM cows that required additional treatments of PG were substantially lower than the TDM group (ODM: 39%; TDM: 64%) than the TDM cows during the initial 21-d period. However, during the 2-wk treatment period, cows in the ODM group produced 26% less milk and 25% less energy-corrected milk than the TDM cows. During wk 3 to 15, when all cows were milked twice daily, ODM cows produced less milk (-14%) and energy-corrected milk (-12%) compared with the TDM group. Milk protein percentage was greater, and milk fat percentage and linear score tended to be greater in the ODM group over 15 wk. In conclusion, a 2-wk reduction of MF in ketotic cows from twice to once daily with treatment with PG resolved ketosis and decreased blood BHB concentrations more effectively than treating TDM cows with PG alone. However, the 2-wk MF reduction had immediate and long-term (up to 13 wk after cessation of MF reduction) negative effects on milk production.
Assuntos
Cetose , Lactação , Ácido 3-Hidroxibutírico , Animais , Bovinos , Indústria de Laticínios , Feminino , Cetose/tratamento farmacológico , Cetose/veterinária , Leite , Proteínas do LeiteRESUMO
The objective of this study was to benchmark the herd-level housing and management strategies of automated milking system (AMS) farms across Canada and assess the associations of these herd-level housing factors and management practices with milk production and quality. Canadian AMS farms (n = 197; Western Canada: n = 50, Ontario: n = 77, Quebec: n = 59, Atlantic Canada: n = 11) were each visited once from April to September 2019, and details were collected related to barn design and herd management practices. Milk-recording data for the 6 mo before farm visits were collected. Farms averaged (± standard deviation) 110 ± 102 lactating cows, 2.4 ± 1.9 AMS units/farm, 47.5 ± 14.9 cows/AMS, 36.7 ± 5.0 kg/d of milk, 4.13 ± 0.34% fat, 3.40 ± 0.16% protein, and a herd-average somatic cell count of 186,400 ± 80,800 cells/mL. Farms mainly used freestall housing systems (92.5%), organic bedding substrates (73.6%), and free flow cow traffic systems (87.8%); farms predominantly milked Holsteins (90.4%). Multivariable regression models were used to associate herd-level housing factors and management practices with milk production and quality. At the herd level, feed push-up frequency (mean = 12.8 ± 8.3 times per day) and feed bunk space (mean = 64 ± 21.5 cm/cow) were positively associated with milk yield. Greater milk yield was associated with herds using inorganic (sand) versus organic bedding, milking Holsteins versus non-Holsteins, and using a form of mechanical ventilation versus natural ventilation alone. Milk fat and milk protein content were only associated with breed. Herds with lower somatic cell counts had more frequent alley cleaning (mean = 12.1 ± 7.5 times per day), wider lying alleys (mean = 304.5 ± 40.0 cm), and sand bedding. The results highlight the importance of using sand bedding, using mechanical ventilation, keeping feed pushed up, ensuring alleys are clean, and ensuring adequate space at the feed bunk for maintaining herd-level productivity and milk quality in farms with AMS.
Assuntos
Indústria de Laticínios , Lactação , Animais , Benchmarking , Bovinos , Fazendas , Feminino , Abrigo para Animais , Ontário , QuebequeRESUMO
Selective antimicrobial treatment strategies present a means to reduce antimicrobial use at the time of arrival at a veal or dairy beef operation. On-farm machine leukocyte differential cell counts (DCC) that can be acquired quickly may be useful to augment calf risk identification protocols. The objective of this study was to assess the utility of DCC taken at the time of arrival at a grain-fed veal facility and 72 h postarrival for determining morbidity risk, mortality risk, and growth during the production cycle. Data were collected between June and October 2018 from 240 calves upon arrival and from a subset of 160 calves 72 h postarrival at a commercial grain-fed veal facility in Ontario, Canada. Blood samples were evaluated using the QScout BLD test for leukocyte DCC (Advanced Animal Diagnostic, Morrisville, NC). All calves were screened using a standardized health examination, and a blood sample was collected to evaluate serum total protein and DCC. Cox proportional hazards models were constructed for both morbidity and mortality outcomes. Mixed linear regression models were constructed to evaluate average daily gain. Results from data collected at the time of arrival suggest that total protein values ≥5.2 g/dL reduced the hazard of mortality and that a rectal temperature >39.6°C was associated with an increased hazard of morbidity. Calves that were dehydrated gained less, whereas calves with an increased lymphocyte count had a higher rate of growth. Results from DCC collected 72 h postarrival suggest that lymphocyte counts between 4.8 and 5.8 × 109 cells/L decreased the hazard of mortality and counts >7.0 × 109 cells/L decreased the hazard of morbidity, whereas neutrophil counts >6.0 × 109 cells/L increased the hazard of mortality. This study demonstrates that machine DCC at the time of arrival and 72 h after arrival has potential for use in identifying high-risk calves that might require treatment, as part of selective antimicrobial therapy protocols, with the purpose of reducing antimicrobial use without sacrificing animal health in veal facilities.
Assuntos
Ração Animal , Doenças dos Bovinos/mortalidade , Indústria de Laticínios , Grão Comestível , Contagem de Leucócitos/veterinária , Carne Vermelha , Animais , Bovinos , Estudos de Coortes , Fazendas , Nível de Saúde , Masculino , Morbidade , Neutrófilos , Ontário , Valor Preditivo dos Testes , Estudos Prospectivos , Fatores de RiscoRESUMO
The objective of this study was to determine the effects of adding molasses-based liquid feed to a high-straw dry cow diet on intake, behavior, health, and performance of dairy cows across the transition period. Holstein cows (n = 40) entering their second lactation or greater were enrolled at dry-off (â¼45 d before expected calving) and assigned to 1 of 2 treatments: a high-straw dry cow total mixed ration (TMR) with either (1) no molasses [CON; n = 20; 10.7% crude protein (CP), 1.45 Mcal/kg of net energy for lactation (NEL)] or (2) supplemented with 1 kg/cow daily [dry matter (DM) basis] of molasses-based liquid feed (LF; n = 20; 11.2% CP, 1.49 Mcal/kg of NEL). At calving all cows were fed the same lactating TMR (14.9% CP, 1.68 Mcal/kg of NEL) and followed for 28 d. During the dry period, cows fed the LF diet had greater DM intake (15.3 ± 0.24 vs. 13.4 ± 0.24 kg/d) and tended to have a shorter interval between meals (192.8 ± 8.38 vs. 216.5 ± 7.97 min) and more frequent meals (6.3 ± 0.27 vs. 5.6 ± 0.27 meals/d), while consuming their feed faster (0.09 ± 0.001 vs. 0.08 ± 0.001 kg of DM/min) compared with cows fed the CON diet. Regardless of treatment, cows sorted [100% × (actual intake/predicted intake)] against the longest ration particles (>19 mm), with cows fed the CON diet sorting more against these (81.1 ± 2.6 vs. 93.9 ± 2.6%). Cows fed the CON diet did not sort for or against short particles (<8, >4 mm; 100.2 ± 0.31%), but cows fed the LF diet tended to sort against these (99.2 ± 0.31%). Post-calving, cows fed the LF diet did not sort for long particles (98.5 ± 1.1%), but cows fed the CON diet continued to sort against these (96.3 ± 1.1%). Cows fed the LF diet had higher mean reticulorumen pH in the dry period (6.4 ± 0.05 vs. 6.2 ± 0.06 pH), in the first week after calving (CON = 5.7 ± 0.06 pH, LF = 5.8 ± 0.06 pH), and tended to have higher mean reticulorumen pH in the second week after calving (CON = 5.7 ± 0.06 pH, LF = 5.9 ± 0.06 pH). The results suggest that supplementing a molasses-based liquid feed in high-straw dry cow diets may improve intake and consistency in nutrients consumed during the dry period and in early lactation, as well as possibly promoting better rumen health across the transition period.
Assuntos
Ração Animal , Bovinos/crescimento & desenvolvimento , Suplementos Nutricionais , Melaço , Animais , Dieta/veterinária , Feminino , Lactação , Leite/metabolismo , Rúmen/metabolismoRESUMO
This retrospective cross-sectional study was designed to identify risk factors associated with failed transfer of passive immunity (FTPI) and to determine differences in colostrum management between male and female dairy calves. Research technicians visited a total of 16 commercial dairy farms weekly. For each calf born on these farms, the farm personnel completed a birth record to document the colostrum management practices provided, level of calving assistance, calf sex, and time of birth. On the weekly visits to the farms, the technicians collected blood from calves that were 1 to 7 d of age. Serum was separated via centrifugation and the concentration of serum total protein (STP) was determined using a digital refractometer. Failed transfer of passive immunity was defined as calves having an STP of <5.2 g/dL. Data were available for 1,778 calves aged 1 to 7 d. Several differences were observed with respect to how male and female calves were managed. Male calves were more likely to receive a lower volume of colostrum, have colostrum delivered using a nipple bottle followed by an esophageal tube feeder, be fed pooled colostrum, and receive fresh colostrum rather than frozen colostrum relative to female calves. Serum total protein (STP) ranged from 3.6 to 9.7 g/dL with a mean of 5.7 g/dL (standard deviation, 0.7 g/dL) and 21.1% of the calves had FTPI. Using a mixed linear regression model, we identified that a calf being male (-0.14 g/dL), being delivered by a hard pull (-0.23 g/dL), and receiving the first feeding colostrum from a combination of a nipple bottle followed by an esophageal tube feeder (-0.12 g/dL) were associated with a lower concentration of STP. Feeding 6 L or more of colostrum in the first 24 h of life was associated with a 0.14 g/dL higher concentration of STP compared with feeding <3.9 L of colostrum. For FTPI, being delivered by a hard pull [odds ratio (OR) 2.21] and receiving the first feeding colostrum from a nipple bottle followed by an esophageal tube feeder (OR 1.83) were associated with higher odds of FTPI. Feeding >6 L of colostrum in the first 24 h of life was associated with a reduced odds (OR 0.65) of FTPI compared with feeding <3.9 L of colostrum. This study highlights the importance of certain management practices in reducing FTPI incidence and identifies discrepancies in colostrum management between male and female dairy calves.
Assuntos
Bovinos/imunologia , Colostro/imunologia , Imunidade Materno-Adquirida , Animais , Animais Recém-Nascidos , Estudos Transversais , Fazendas , Feminino , Imunoglobulina G/sangue , Masculino , Razão de Chances , Gravidez , Refratometria , Estudos Retrospectivos , Fatores de RiscoRESUMO
The primary objective of this prospective cohort study was to identify factors evaluated upon arrival at a grain-fed veal facility that were associated with increased growth. A secondary objective was to determine if the factors associated with average daily gain (ADG) varied between the pre- and postweaning periods. Calves were visually assessed and scored using a standardized health scoring system immediately upon arrival. They were also weighed and vaccinated, and had blood drawn from their jugular vein to determine their serum total protein level. Weights were taken on d 49 and 78 after arrival and were used to calculate ADG. Three mixed linear regression models were created to determine factors associated with ADG from 0 to 49 d after arrival, 0 to 78 d after arrival, and 49 to 78 d after arrival. A total of 998 calves were evaluated and weighed upon arrival; 636 and 915 of those calves were weighed at d 49 and d 78, respectively. Mean ADG were 610 g/d from 0 to 49 d after arrival, 860 g/d from 0 to 78 d after arrival, and 1,240 g/d from 49 to 78 d after arrival. In the model evaluating growth from 0 to 49 d, calves arriving at the facility with a higher body mass index (BMI) gained more weight per day than calves that arrived with a lower BMI. If calves arrived in the spring, they gained 201 g/d more than calves that arrived in the winter. For the period following weaning (d 49 to 78), BMI was also associated with growth. For every additional 1 g/cm2 increase in BMI at arrival, calves gained an additional 1.55 g/d from d 49 to 78. Growth from arrival to d 78 was affected solely by BMI. For every 1 g/cm2 increase in BMI at arrival, calves gained 1.23 g/d more during the entire observation period. Body mass index and season at arrival affected growth through the initial period at a grain-fed veal facility. Producers should consider these factors, especially BMI, when purchasing calves to maximize the calves' growth potential.
Assuntos
Ração Animal , Bovinos/crescimento & desenvolvimento , Animais , Animais Recém-Nascidos/crescimento & desenvolvimento , Índice de Massa Corporal , Estudos de Coortes , Dieta/veterinária , Grão Comestível , Nível de Saúde , Masculino , Estudos Prospectivos , Carne Vermelha , Fatores de Risco , Estações do Ano , Desmame , Aumento de PesoRESUMO
The objective of this study was to determine the effect of adding water to a high-straw dry cow diet on feeding behavior, health, and performance of dairy cows. Holstein cows (n = 40) entering their second or greater lactation were enrolled at dry off (â¼45 d before expected calving) and assigned to 1 of 2 dietary treatments, consisting of a high-straw dry cow total mixed ration [TMR; 36% wheat straw, 41% corn silage, 23% pellet, on a dry matter (DM) basis, formulated for 11.6% crude protein, 1.35 Mcal/kg net energy for lactation] with (1) no water (CON; n = 20; DM = 53.4%) or (2) water added to decrease the DM by â¼10 percentage points (wet diet, WD; n = 20; DM = 45.4%). Upon calving, all cows were fed the same lactating TMR (45.2% DM, 14.9% crude protein, 1.68 Mcal/kg net energy for lactation) and followed for 28 d. Dry matter intake, feeding behavior, and rumination activity were recorded automatically. Fresh TMR and orts samples were collected 2×/wk to determine differences in sorting. A particle separator was used to separate feed samples into 4 fractions: long (>19 mm), medium (<19, >8 mm), short (<8, >4 mm), and fine (<4 mm) particles. Feed sorting was calculated as actual intake of each particle fraction expressed as a % of predicted intake. Cows fed WD had greater DM intake during the dry period than cows fed CON (CON = 13.4 ± 0.24, WD = 14.2 ± 0.24 kg/d), but rumination did not differ (CON = 522.2 ± 8.48, WD = 518.5 ± 8.69 min/d) between treatments. Cows fed WD tended to consume their feed faster (CON = 0.08 ± 0.001, WD = 0.09 ± 0.001 kg of DM/min) compared with CON cows. Regardless of treatment, cows sorted against the longest ration particles, with cows fed CON sorting more against these (CON = 81.1 ± 2.51%, WD = 94.9 ± 2.58%). Cows fed CON tended to sort more in favor of the medium particles than those fed WD (CON = 105.0 ± 0.36%, WD = 104.2 ± 0.37%). Postcalving DM intake and feeding behavior were not affected by dry diet treatment. Cows fed WD experienced a less rapid daily decline in rumen pH during the first 7 d after calving compared with those fed the CON diet. The results suggest that increasing the moisture content of high-straw dry cow diets, by adding water, may improve intake and reduce sorting against the longest ration particles, which may help promote consistency in targeted nutrients consumed during the dry period and greater rumen health in early lactation.
Assuntos
Bovinos/fisiologia , Ingestão de Alimentos , Comportamento Alimentar , Silagem/análise , Triticum , Água/fisiologia , Bem-Estar do Animal , Animais , Dieta/veterinária , Feminino , Lactação , Rúmen/metabolismo , Zea maysRESUMO
The study investigated the effect of chop length of wheat straw in a high-straw dry cow diet on the feeding behavior, health, and performance of Holstein dairy cows across the transition period. Forty cows were enrolled at dry off, approximately 45 d before expected calving, and assigned to 1 of 2 dietary treatments containing (on a dry matter basis) 36% corn silage, 35% dry cow pellet, and 29% wheat straw. Diets were formulated to target an energy consumption of 19 Mcal/d of net energy for lactation per cow. The diets differed in the chop length of wheat straw: (1) long straw chopped with a 10.16-cm screen (LDD; n = 20), or (2) short straw chopped with a 2.54-cm screen (SDD; n = 20). Dietary treatments were fed throughout the dry period. Feeding behavior, rumination time, and reticulorumen pH were monitored electronically, and feed samples were collected to determine feed sorting. Blood samples were taken weekly, and body condition scores and body weights were taken every 2 wk. Postcalving, all cows were fed the same lactating cow total mixed ration for 28 d; the same measurements were recorded with the addition of daily milk yield and twice weekly milk components. During the dry period, cows fed the SDD treatment consumed more dry matter (15.6 vs. 15.0 kg/d), had more consistent dry matter intake in the week leading up to calving, had a faster feeding rate, consumed 0.4 kg/meal more, and had 0.6 fewer meals per day. No differences were observed in rumination time or mean reticulorumen pH between treatments in both the dry and lactating period. Regardless of treatment during the dry period, cows sorted against the long particles (>19 mm) and in favor of the short particles (<8, >4 mm), but cows fed the LDD treatment sorted to a greater extent than cows fed the SDD treatment. Dietary treatment in the dry period did not influence feed sorting behavior postcalving. Cows fed the LDD treatment had a greater daily change (decline) in rumen pH in the first week postcalving and had a greater blood ß-hydroxybutyrate concentration in the third week postcalving. Overall, the results from this study indicate that feeding shorter chopped wheat straw in a high-straw dry cow diet improved intake, reduced sorting during the dry period, and resulted in greater metabolic health and rumen stability in early lactation.