Long wavelength-sensing cones of zebrafish retina exhibit multiple layers of transcriptional heterogeneity.

Introduction: Understanding how photoreceptor genes are regulated is important for investigating retinal development and disease. While much is known about gene regulation in cones, the mechanism by which tandemly-replicated opsins, such as human long wavelength-sensitive and middle wavelength-sensitive opsins, are differentially regulated remains elusive. In this study, we aimed to further our understanding of transcriptional heterogeneity in cones that express tandemly-replicated opsins and the regulation of such differential expression using zebrafish, which express the tandemly-replicated opsins lws1 and lws2. Methods: We performed bulk and single cell RNA-Seq of LWS1 and LWS2 cones, evaluated expression patterns of selected genes of interest using multiplex fluorescence in situ hybridization, and used exogenous thyroid hormone (TH) treatments to test selected genes for potential control by thyroid hormone: a potent, endogenous regulator of lws1 and lws2 expression. Results: Our studies indicate that additional transcriptional differences beyond opsin expression exist between LWS1 and LWS2 cones. Bulk RNA-Seq results showed 95 transcripts enriched in LWS1 cones and 186 transcripts enriched in LWS2 cones (FC > 2, FDR < 0.05). In situ hybridization results also reveal underlying heterogeneity within the lws1- and lws2-expressing populations. This heterogeneity is evident in cones of mature zebrafish, and further heterogeneity is revealed in transcriptional responses to TH treatments. Discussion: We found some evidence of coordinate regulation of lws opsins and other genes by exogenous TH in LWS1 vs. LWS2 cones, as well as evidence of gene regulation not mediated by TH. The transcriptional differences between LWS1 and LWS2 cones are likely controlled by multiple signals, including TH.

The impact of resistance training on strength and correlates of physical activity in youth.

Resistance training (RT) may have a positive impact on specific correlates of physical activity (PA) in inactive and/or obese youth, with strength as a possible underlying mechanism. The aim of this study was to investigate this. Twelve participants (aged 8.9 ± 1.0 years) were assigned to an experimental group (EG) or control group (CG). Pre and post intervention assessments for strength, physical self-perceptions (PSPs), weight status, fundamental movement skills (FMS), and PA levels were completed. The EG participated in a twice-weekly 10-week RT programme. There were significant group x time interactions for FMS (CAMSA total P = 0.016, CAMSA skill score P = 0.036) and stretch stature (P = 0.002) (EG larges changes than the CG). Large effect sizes for the differences in change scores between the EG and CG were evident for CAMSA total score (Hedges' g = 0.830, P = 0.138), CAMSA skill score (Hedges' g = 0.895, P = 0.112) and relative strength (Hedges' g = 0.825, P = 0.140). This study demonstrated that a 10-week RT intervention has a positive effect on strength and FMS, and may also benefit weight status and PSPs. This study supports the development of RT interventions to develop these correlates, and increase PA levels.

The Effect of Resistance Training Interventions on 'The Self' in Youth: a Systematic Review and Meta-analysis.

BACKGROUND: There is growing evidence that physical activity (PA) is beneficial for the mental health of young people. One area that has been widely examined is the impact of PA on 'the self', which is a term that encompasses a range of specific and related terms (e.g. self-esteem, self-efficacy, self-perceptions). There is evidence that PA is strongly associated with 'the self' in childhood and beyond. However, the impact of the specific PA of resistance training (RT) is not yet clear. The purpose of this review was to advance knowledge on the potential of RT for enhancing mental health by examining the effect of RT interventions on 'the self' in youth. METHODS: This systematic review followed the PRISMA guidelines (PROSPERO registration number CRD42016038365). Electronic literature databases were searched from the year of their inception to October 2018. The search included English language articles that examined the effect of isolated RT on the broad term of 'the self' in youth, with participants of school age (5-18 years). Data were extracted using an electronic form by one reviewer with 10% conducted by a second reviewer. The 'Quality Assessment Tool for Quantitative Studies' was used to assess the quality and risk of bias and was conducted by two reviewers. RESULTS: From seven peer-reviewed studies, ten data sets were included exploring seven outcomes related to 'the self' in participants aged between 10 and 16 years. Four of these studies (including seven data sets) were combined in a meta-analysis, with results from the remaining three studies reported separately. Significant intervention effects were identified for resistance training self-efficacy (Hedges' g = 0.538, 95% CI 0.254 to 0.822, P < 0.001), physical strength (Hedges' g = 0.289, 95% CI 0.067 to 0.511, P = 0.011), physical self-worth (Hedges' g = 0.319, 95% CI 0.114 to 0.523, P = 0.002) and global self-worth (Hedges' g = 0.409, 95% 0.149 to 0.669, P = 0.002). Although not statistically significant, the effect sizes for the remaining three outcomes were body attractiveness (Hedges' g = 0.211, 95% CI - 0.031 to 0.454, P = 0.087), physical condition (Hedges' g = 0.089, 95% CI - 0.238 to 0.417, P = 0.593) and sport competence (Hedges' g = 0.004, 95% CI - 0.218 to 0.225, P = 0.974). There was variable quality of studies, with just two studies being classified as 'strong'. CONCLUSION: This is the first review to synthesise research on the effects of isolated RT interventions on 'the self'. The findings indicate that RT has a positive impact on some aspects of 'the self' in youth. More high-quality studies should be conducted to further investigate this topic. If validated, this type of intervention could have a positive impact on 'the self' and ultimately improve the health of individuals not only during childhood but as they progress through life.

The effect of resistance training interventions on fundamental movement skills in youth: a meta-analysis.

BACKGROUND: Fundamental movement skills (FMS) are strongly related to physical activity (PA) in childhood and beyond. To develop FMS, resistance training (RT) may be a favourable intervention strategy. The purpose of this meta-analysis was to systematically examine the effect of RT interventions on FMS in youth. METHODS: Meta-analysis followed the PRISMA guidelines (Prospero registration number CRD42016038365). Electronic literature databases were searched from the year of their inception up to and including June 2017. The search strategy aimed to return studies that included product and process-oriented measures as a means of assessing FMS. Studies from English language peer-reviewed published articles that examined the effect of RT on indicators of FMS in youth, with participants of school age (5-18 years) were included. RESULTS: Thirty-three data sets were included exploring five outcomes related to FMS. Studies included only reported product-oriented outcomes. Significant intervention effects were identified for: sprint (Hedges' g = 0.292, 95% CI 0.017 to 0.567, P = 0.038), squat jump (Hedges' g = 0.730, 95% CI 0.374 to 1.085, P = < 0.001), standing long jump (Hedges' g = 0.298, 95% CI 0.096 to 0.499, P = 0.004), throw (Hedges' g = 0.405, 95% CI 0.094 to 0.717, P = 0.011) and vertical jump (Hedges' g = 0.407, 95% CI 0.251 to 0.564, P = < 0.001). There was variable quality of studies, with 33.3% being classified as 'strong'. CONCLUSION: RT has a positive impact on indicators of FMS in youth but more high-quality studies should be conducted to further investigate the role RT may play in the development of FMS. Additionally, to more comprehensively evaluate the impact of RT on FMS, there is a need for FMS assessments that measure both process- and product-oriented outcomes.

The effect of resistance training interventions on weight status in youth: a meta-analysis.

BACKGROUND: There has been a rise in research into obesity prevention and treatment programmes in youth, including the effectiveness of resistance-based exercise. The purpose of this meta-analysis was to examine the effect of resistance training interventions on weight status in youth. METHODS: Meta-analysis followed the Preferred Reporting Items for Systematic Reviews and Meta-Analyses guidelines and was registered on PROSPERO (registration number CRD42016038365). Eligible studies were from English language peer-reviewed published articles. Searches were conducted in seven databases between May 2016 and June 2017. Studies were included that examined the effect of resistance training on weight status in youth, with participants of school age (5-18 years). RESULTS: There were 24 complete sets of data from 18 controlled trials (CTs) which explored 8 outcomes related to weight status. Significant, small effect sizes were identified for body fat% (Hedges' g = 0.215, 95% CI 0.059 to 0.371, P = 0.007) and skinfolds (Hedges' g = 0.274, 95% CI 0.066 to 0.483, P = 0.01). Effect sizes were not significant for: body mass (Hedges' g = 0.043, 95% CI - 0.103 to 0.189, P = 0.564), body mass index (Hedges' g = 0.024, 95% CI - 0.205 to 0.253, P = 0.838), fat-free mass (Hedges' g = 0.073, 95% CI - 0.169 to 0.316, P = 0.554), fat mass (Hedges' g = 0.180, 95% CI - 0.090 to 0.451, P = 0.192), lean mass (Hedges' g = 0.089, 95% CI - 0.122 to 0.301, P = 0.408) or waist circumference (Hedges' g = 0.209, 95% CI - 0.075 to 0.494, P = 0.149). CONCLUSIONS: The results of this meta-analysis suggest that an isolated resistance training intervention may have an effect on weight status in youth. Overall, more quality research should be undertaken to investigate the impact of resistance training in youth as it could have a role to play in the treatment and prevention of obesity.

Interaction of pre-programmed control and natural stretch reflexes in human landing movements.

Pre-programmed mechanisms of motor control are known to influence the gain of artificially evoked stretch reflexes. However, their interaction with stretch reflexes evoked in the context of unimpeded natural movement is not understood. We used a landing movement, for which a stretch reflex is an integral part of the natural action, to test the hypothesis that unpredicted motor events increase stretch reflex gain. The unpredicted event occurred when a false floor, perceived to be solid, collapsed easily on impact, allowing the subjects to descend for a further 85 ms to a solid floor below. Spinal stretch reflexes were measured following solid floor contact. When subjects passed through the false floor en route to the solid floor, the amplitude of the EMG reflex activity was double that found in direct falls. This was not due to differences in joint rotations between these conditions. Descending pathways can modify H- and stretch-reflex gain in man. We therefore manipulated the time between the false and real floor contacts and hence the time available for transmission along these pathways. With 30 ms between floors, the enhancement of the reflex was extinguished, whereas with 50 ms between floors it reappeared. This excluded several mechanisms from being responsible for the doubling of the reflex EMG amplitude. It is argued that the enhanced response is due to the modulation of reflex gain at the spinal level by signals in descending pathways triggered by the false platform. The results suggest the future hypothesis that this trigger could be the absence of afferent signals expected at the time of false floor impact and that salient error signals produced from a comparison of expected and actual sensory events may be used to reset reflex gains.