Adult rhabdomyoma of the larynx.

Rhabdomyomas of the larynx are rare lesions, with the "adult" type occurring more frequently than the "fetal" type. Treatment is complete surgical removal. Recurrences would suggest an incomplete surgical procedure and/or an incorrect pathological diagnosis.

Olfactory neuroblastoma.

Despite the fact that olfactory neuroblastoma is considered to be a rare malignant neoplasm of the sinonasal cavity, its frequent inclusion in the differential diagnosis of small-cell neoplasms of the nasal cavity is commonplace. Differential diagnosis of small round tumors solely on the basis of a light-microscopic examination of a hematoxylin and eosin stain often can be impossible. However, immunohistochemical stains such as NSE, S-100, and chromogranin immunostain can be very helpful in diagnosing olfactory neuroblastoma. If special stains are noncontributory, the diagnosis may hinge on supporting evidence from a series of electron micrographs of a properly selected and prepared tumor.

Common variable hypogammaglobulinemia complicated by an unusual T-suppressor/cytotoxic cell lymphoma.

A patient with common variable hypogammaglobulinemia (CVH) who presented with recurrent sinopulmonary infections, nodular lymphoid hyperplasia of the small bowel, and intestinal giardiasis was studied. A diffuse lymphocytic lymphoma with small bowel, skin, and hepatic involvement subsequently developed in the patient. Light microscopy of the tumor revealed tissue infiltration with mononuclear cells having the morphologic features of T-lymphocytes. The malignant lymphocytes had characteristics of T-suppressor/cytotoxic cells as established by the absence of surface immunoglobulin and Leu 3 surface markers, and the presence of OKT3 and OKT8 surface markers. Peripheral blood lymphocyte studies revealed an increased number of T-suppressor cells, a reversal of the helper-suppressor ratio, and a generalized state of hyporesponsiveness to mitogen and antigen stimulation. No evidence of retroviral reverse transcriptase activity was detected in cultures of peripheral blood lymphocytes. The association between CVH and a lymphoma composed of cells with T-suppressor/cytotoxic surface markers has not been previously reported. The postulated role of T-suppressor cells in the failure of immunoglobulin synthesis in some forms of CVH suggests that the finding of a T-suppressor/cytotoxic cell lymphoma complicating CVH may be more than fortuitous.

Sodium-dependent transport of inorganic sulfate by rabbit renal brush-border membrane vesicles. Effects of other ions.

A Na+ gradient (extravesicular greater than intravesicular) increased the rate of inorganic sulfate (SO24-) uptake into renal brush-border membrane vesicles and energized the transient accumulation of the anion against its concentration gradient, indicating a secondary active transport system. Stimulation of SO24- uptake was specific for Na+. The anions, SO23-, S2O23-, SeO24-, MoO24-, CrO24-, and WO24-, but not HPO24-, cis-inhibited and trans-stimulated SO24- uptake, suggesting that these divalent anions shared the SO24- carrier. The Na+/SO24- co-transport and Na+. The apparent Km for SO24- was 0.6 mM at 100 mM Na+. The relationship between Na+ concentration and rate of SO24- uptake was sigmoidal. From a Hill analysis of the data a [Na+]0.5 of 36 mM and an n value of 1.6 were calculated. Comparisons of the effects of a K+ diffusion potential (inside positive), of a H+ diffusion potential (inside negative), of Na+ salts of anions of different conductances on the Na+-dependent uptakes of SO24- and D-glucose, and of the responses of a membrane potential-sensitive fluorescent probe concomitant with the uptakes indicate that Na+/SO24- co-transport was electroneutral. The simplest stoichiometry consistent with an electroneutral mechanism would be the co-transport of two Na+ and one SO24-. Na+ gradient-dependent SO24- uptake was enhanced by intravesicular Cl-. cis-Cl- inhibited the efflux as well as the influx of SO24-. These findings suggest that Cl- was an inhibitor of SO24- transport. Intravesicular K+ stimulated Na+ gradient-dependent SO24- uptake. The co-transport of Na+/SO24- appeared not to be coupled to the transmembrane flux of K+. It is hypothesized that the co-transport system contained an internal site activated by K+.