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J Invertebr Pathol ; 108(2): 126-30, 2011 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-21843526

RESUMO

Phagocytosis of pathogens by hemocytes is a rapid-acting immune response and represents a primary means of limiting microbial infection in some species of arthropods. To survey the relative capacity of hemocyte phagocytosis as a function of the arthropod immune response, we examined the extent of phagocytosis among a wide taxonomic range of arthropod species including a decapod crustacean (Litopenaeus vannamei), three ixodid tick species (Amblyomma americanum, Dermacentor variabilis, and Ixodes scapularis), a mosquito species (Aedes aegypti), and a larval moth (Manduca sexta). Injected fluorescent beads were used as a model to elicit phagocytosis and were measured by flow cytometry, a technique provided in detail that may be adapted for use with any species of arthropod. The data indicated that smaller arthropods generally had a higher proportion of phagocytic cells than larger arthropods.


Assuntos
Artrópodes/fisiologia , Citometria de Fluxo/métodos , Hemócitos/imunologia , Interações Hospedeiro-Patógeno/imunologia , Fagocitose/fisiologia , Animais , Contagem de Células , Feminino , Hemócitos/citologia , Hemolinfa/imunologia
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