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Arthropod Struct Dev ; 47(1): 74-81, 2018 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-29199047

RESUMO

For providing their offspring females of the digger wasp species Ampulex compressa hunt cockroaches, paralyze them and attach as a rule one egg to the coxa of one of the mid legs of their prey. We observed the egg-laying behavior and examined with light- and scanning microscopy (i) nearly mature eggs from ovaries of freshly dissected females and (ii) eggs immediately after their deposition on the coxae of their prey. The length of the white bean-shaped eggs varied between 2.2 and 3.0 mm, their diameter between 0.66 and 0.72 mm, and their weight between 345 and 832 µg. The surface of fresh, untreated eggs shows even at higher magnifications (>20.000×) a smooth appearance. However, after conventional fixation, dehydration with ethyl-alcohol and critical-point drying the egg-surface exhibited a little bit texture. The eggs are at two-third of their underside glued to the coxa of the prey. With the naked eye the glue appears as a compact mass. The eggs may be mechanically removed from the substrate (their attachment site); however, in doing so the viscous attachment glue appears in a more fibrous consistence. The polypeptide composition washed off the egg surface and the glue revealed no similarities, whereas the molecular mass of two polypeptides were similar between glue and the Dufour's gland contents.


Assuntos
Oviposição , Óvulo/química , Vespas/anatomia & histologia , Vespas/fisiologia , Animais , Eletroforese em Gel de Poliacrilamida , Proteínas de Insetos/análise , Microscopia Eletrônica de Varredura , Óvulo/citologia , Óvulo/ultraestrutura , Peptídeos/análise , Vespas/ultraestrutura
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