The Nephroprotective Effect of In Utero Administration of Green Synthesized Titanium Dioxide Nanoparticles in Albino Rats.

Titanium dioxide nanoparticles (TiO2-NPs) are one of the most popular nanoscale materials and have a wide range of applications in the manufacturing industry; nonetheless, researchers' focus has been directed to the detrimental consequences of TiO2-NPs. The current study was designed to assess the potential hazardous effects of chemically synthesized TiO2-NPs on the placenta and feto-maternal kidneys of rats. On the other hand, the probable positive impact of TiO2-NPs made after green synthesis was also evaluated. HepG2 cell lines were used to assess the cytotoxicity of chemical and green TiO2-NPs. Five groups of fifty pregnant female rats were formed (n=10). The first (control) group received distilled water. The second and third groups were orally given 100 and 300 mg/kg body weight (bw) of chemical TiO2-NPs, respectively. The fourth and fifth groups were orally given 100 and 300 mg/kg bw of green synthesized TiO2-NPs, respectively. On gestational day 20 (GD 20), blood and tissues were collected for biochemical and histological studies. Our findings revealed that chemical TiO2-NPs induced apoptosis in HepG2 cells at high concentrations, while there was no observed toxicity for green TiO2-NPs. The chemically treated TiO2-NPs groups showed a significant decrease in the level of HDL and a significant increase in cholesterol, LDL-cholesterol, and triglyceride levels. Renal tissues showed necrosis with exfoliation of lining epithelial cells, degenerated tubules, and glomerulonephritis. While the placenta was atrophied and hyalinized. Moreover, Bax expression significantly increased in the renal tubular cells and the villi of the placenta. Contrariwise, green TiO2-NPs-treated groups showed a significant rise in HDL levels with a significant reduction in triglycerides and LDL levels, while cholesterol levels were unaffected. Also, renal tissues showed mild degenerative changes in the glomeruli and renal tubules; thus, noticeable regeneration of epithelium lining tubules was detected in the maternal kidney. Bax showed a minimal reaction in the renal tubules and the villi of the placenta. It concluded that in contrast to chemical TiO2-NPs, biosynthesized TiO2-NPs with garlic showed a positive impact on the biochemical profile and histological investigations.

Exploration of PVC@SiO2 nanostructure for adsorption of methylene blue via using quartz crystal microbalance technology.

Methylene blue (MB) dye is considered a well-known dye in many industries and the low concentration of MB is considered very polluted for all environment if it discharged without any treatment. For that reason, many researchers used advanced technologies for removing MB such as the electrochemical methods that considered very simple and give rapid response. Considering these aspects, a novel quartz crystal microbalance nanosensors based on different concentrations of PVC@SiO2 were designed for real-time adsorption of MB dye in the aqueous streams at different pHs and different temperatures. The characterization results of PVC@SiO2 showed that the PVC@SiO2 have synthesized in spherical shape. The performance of the designed QCM-Based PVC@SiO2 nanosensors were examined by the QCM technique. The sensitivity of designed nanosensors was evaluated at constant concentration of MB (10 mg/L) at different pHs (2, 7 and 11) and temperatures (20 °C, 25 °C, and 30 °C). From the experimental, the best concentration of PVC@SiO2 was 3% for adsorbed 9.99 mg of cationic methylene blue at pH 11 and temperature 20 °C in only 5.6 min.

Genetic effects of chemically and biosynthesized titanium dioxide nanoparticles in vitro and in vivo of female rats and their fetuses.

With the increase in nanoparticles (NPs) products on the market, the possibility of animal and human exposure to these materials will increase. The smaller size of NPs facilitates their entrance through placental barriers and allows them to accumulate in embryonic tissue, where they can then be a source of different developmental malformations. Several toxicity studies with chemically synthesized titanium dioxide NPs (CTiO2 NPs) have been recently carried out; although there is insufficient data on exposure to biosynthesized titanium dioxide NPs (BTiO2 NPs) during pregnancy, the study aimed to evaluate the ability of an eco-friendly biosynthesis technique using garlic extract against maternal and fetal genotoxicities, which could result from repeated exposure to TiO2 NPs during gestation days (GD) 6-19. A total of fifty pregnant rats were divided into five groups (n = 10) and gavaged CTiO2 NPs and BTiO2 NPs at 100 and 300 mg/kg/day concentrations. Pregnant rats on GD 20 were anesthetized, uterine horns were removed, and then embryotoxicity was performed. The kidneys of the mothers and fetuses in each group were collected and then maintained in a frozen condition. Our results showed that garlic extract can be used as a reducing agent for the formation of TiO2 NPs. Moreover, BTiO2 NPs showed less toxic potential than CTiO2 NPs in HepG2 cells. Both chemically and biosynthesized TiO2 NP-induced genetic variation in the 16S rRNA sequences of mother groups compared to the control group. In conclusion, the genetic effects of the 16S rRNA sequence induced by chemically synthesized TiO2 NPs were greater than those of biosynthesized TiO2 NPs. However, there were no differences between the control group and the embryo-treated groups with chemically and biologically synthesized TiO2 NPs.

Immunomodulatory and antioxidant effect of green synthesized titanium dioxide nanoparticles on pregnant female albino rats and their fetuses.

Titanium dioxide nanoparticles (TiO2 NPs) are one of the various nanoparticles that have been increasingly commonly used in vital sectors. This study was aimed at evaluating the effects of prenatal exposure to the chemical TiO2 NPs (CHTiO2 NPs) and green-synthesized TiO2 NPs (GTiO2 NPs) on immunological and oxidative status as well as lungs and spleen. Fifty pregnant female albino rats were divided into five groups of ten rats each: control, CHTiO2 NPs-treated groups orally received 100 and 300 mg/kg CHTiO2 NPs, and GTiO2 NPs-treated groups received 100 and 300 mg/kg GTiO2 NPs, respectively, daily for 14 days. The serum level of proinflammatory cytokines IL-6, oxidative stress markers (MDA and NO), and antioxidant biomarkers (SOD and GSH-PX) were assayed. Spleen and lungs were collected from pregnant rats and fetuses for histopathological examinations. The results showed a significant increase in IL-6 levels in treated groups. In the CHTiO2 NPs-treated groups, there was a significant increase in MDA activity and a significant decrease in GSH-Px and SOD activities, revealing its oxidative effect, while GSH-Px and SOD activities significantly increased in the 300 GTiO2 NPs-treated group, confirming the antioxidant effect of green-synthesized TiO2 NPs. Histopathological findings of the spleen and lungs of the CHTiO2 NPs-treated group revealed severe congestion and thickening of the blood vessels, while those of the GTiO2 NPs-treated group revealed mild tissue alterations. It could be deduced that green synthesized titanium dioxide nanoparticles have immunomodulatory and antioxidant effects on pregnant female albino rats and their fetuses, with an ameliorated impact on the spleen and lung compared to chemical titanium dioxide nanoparticles.

Behavior of Silica Nanoparticles Synthesized from Rice Husk Ash by the Sol-Gel Method as a Photocatalytic and Antibacterial Agent.

Silica nanoparticles (SiO2 NPs) are one of the most well-studied inorganic nanoparticles for many applications. They offer the advantages of tunable size, biocompatibility, porous structure, and larger surface area. Thus, in this study, a high yield of SiO2 NPs was produced via the chemical treatment of rice husk ash by the sol-gel method. Characteristics of the prepared SiO2 NPs were validated using different characterization techniques. Accordingly, the phase, chemical composition, morphological, and spectroscopic properties of the prepared sample were studied. The average particle size of the SiO2 NPs was found to be approximately 60-80 nm and the surface area was 78.52 m²/g. The prepared SiO2 NPs were examined as photocatalysts for the degradation of methyl orange (MO) dye under UV irradiation. It was found that the intensity of the characteristic absorption band of MO decreased gradually with exposure time increasing, which means the successful photodegradation of MO by SiO2 NPs. Moreover, the antibacterial activity of obtained SiO2 NPs was investigated by counting the coliform bacteria in the surface water using the most probable number (MPN) index method. The results revealed that the MPN of coliform bacteria untreated and treated by SiO2 NPs was estimated to be 170 CFU/100 mL and 10 CFU/100 mL, respectively, resulting in bacterial growth inhibition of 94.12%.

Ameliorative effect of biosynthesized titanium dioxide nanoparticles using garlic extract on the body weight and developmental toxicity of liver in albino rats compared with chemically synthesized nanoparticles.

The application of metallic nanoparticles poses risks to human and animal health. Titanium dioxide nanoparticles (TiO2NPs) are the most commonly synthesized metallic oxides in the world. Exposure to TiO2NPs can cause toxicity in the target organisms. This study aimed to evaluate the effects of green and chemical TiO2NPs on maternal and embryo-fetal livers. Green TiO2NPs using garlic extract (GTiO2NPs) and chemical TiO2NPs (CHTiO2NPs) were synthesized and characterized by x-ray powder diffraction and high-resolution transmission electron microscopy. The cytotoxicity of both chemical and green TiO2NPs was determined against HepG2 cell lines. Fifty pregnant female Albino rats were equally and randomly divided into five groups. Group 1 was kept as a control. Groups 2 and 3 were orally treated with 100 and 300 mg/kg body weight of CHTiO2NPs, respectively. Groups 4 and 5 were orally treated with 100 and 300 mg/kg of GTiO2NPs, respectively, from day 6 to 19 of gestation. All dams were euthanized on gestation day 20. All live fetuses were weighed and euthanized. Blood and tissue samples were collected for biochemical, histopathological, and Bax-immunohistochemical expression analyses. Our results indicated that garlic could be used as a reducing agent for the synthesis of TiO2NPs, and the produced NPs have no toxic effect against HepG2 cells compared with CHTiO2NPs. The maternal and fetal bodyweights were greatly reduced among the chemically TiO2NPs induced animals. The mean serum level of AST and ALT activities and the total protein level significantly increased when TiO2NPs were administered at high doses. Histologically, the CHTiO2NPs-treated groups revealed vacuolated and necrotized hepatocytes with congested and dilated blood vessels in the fetal and maternal livers. The immunohistochemistry revealed distinct positive staining of Bax expressed in the hepatocytes. Nevertheless, the biosynthesis of TiO2NPs using garlic extract had a minimal effect on the normal architecture of the liver. It could be concluded that the bioactivity of TiO2NPs can be modified by green synthesis using garlic extract. Compared to the CHTiO2NPs, the exposure to GTiO2NPs showed reduced liver damage in maternal and embryo-fetal rats.