Simultaneous assessment of radial and axial myocyte mechanics by combining atomic force microscopy and carbon fibre techniques.

Cardiomyocytes sense and shape their mechanical environment, contributing to its dynamics by their passive and active mechanical properties. While axial forces generated by contracting cardiomyocytes have been amply investigated, the corresponding radial mechanics remain poorly characterized. Our aim is to simultaneously monitor passive and active forces, both axially and radially, in cardiomyocytes freshly isolated from adult mouse ventricles. To do so, we combine a carbon fibre (CF) set-up with a custom-made atomic force microscope (AFM). CF allows us to apply stretch and to record passive and active forces in the axial direction. The AFM, modified for frontal access to fit in CF, is used to characterize radial cell mechanics. We show that stretch increases the radial elastic modulus of cardiomyocytes. We further find that during contraction, cardiomyocytes generate radial forces that are reduced, but not abolished, when cells are forced to contract near isometrically. Radial forces may contribute to ventricular wall thickening during contraction, together with the dynamic re-orientation of cells and sheetlets in the myocardium. This new approach for characterizing cell mechanics allows one to obtain a more detailed picture of the balance of axial and radial mechanics in cardiomyocytes at rest, during stretch, and during contraction. This article is part of the theme issue 'The cardiomyocyte: new revelations on the interplay between architecture and function in growth, health, and disease'.

A novel measurement strategy to evaluate the human head as a transition medium for inductive ear-to-ear communication.

This manuscript introduces a novel concept for measuring coil coupling for extremely loose-coupled coils (coupling factors k<10-6; mutual inductance values M<10-10 H). Such a coupling is found everywhere where the ratio of solenoid diameter to coil spacing is >50. Measuring these quantities with a low-power technology requires a sophisticated setup that goes beyond the sensitivity of state-of-the art approaches. The methodology is validated using laboratory measurements with three sets of solenoids (two ferrite-cored, one air-cored) and numerical simulations with COMSOL Multiphysics 5.2a, Stockholm, Sweden. The concept is then employed to investigate the channel characteristics for inductive through-the-head communication within the 3.155-3.195 MHz band. This selected part of the spectrum is in accordance with International Telecommunication Union Radio Regulation 5.116 for low-power wireless hearing aids. By applying a phantom solution, we demonstrate that human tissue layers are transparent for magnetic fields within these frequencies. However, the influence from the relative coil arrangement is evaluated in detail as it restricts the communication range significantly. The coupling results for off-the-shelf Sonion, Roskilde, Denmark, RF 02 AA 10 solenoids considering both lateral and axial displacements might be of special interest for a number of near-field applications.

An ECG simulator with a novel ECG profile for physiological signals.

This work introduces a low-cost open-source electrocardiography (ECG) simulator comprising both MATLAB software for signal generation and a dedicated circuit board for signal output via a commercial sound card. Synthetic, rate-dependent ECG simulation is based on third-order polynomials that are calculated in sections for the main waves and spikes, respectively. Besides the heart rate, the output profile is fully adjustable with respect to Einthoven lead signals I-III, the amplitudes of the individual ECG waves and spikes, as well as the constitution and intensity of common distortions. The underlying coefficients for the synthetic ECG profile are obtained experimentally by analysing recordings of 22 healthy individuals with heart rates in the range of 40-180 bpm. Eight of these recordings are selected to determine the coefficients for the polynomials (training set) while the remaining 14 serve as test set to evaluate their applicability and accuracy. Thereby, a mean correlation of 98.57% is found which is superior in comparison with a widely accepted rate-dependent ECG profile that is generated from square root and linear terms (correlation score: 91.46%). Although other use-cases are feasible, the focus of this work is the development of an ECG simulator for academic research and university education. Both the MATLAB source code and the circuit layout files are available in the online supplement stimulating further work on this topic.

A Bioreactor to Apply Multimodal Physical Stimuli to Cultured Cells.

Cells residing in the cardiac niche are constantly experiencing physical stimuli, including electrical pulses and cyclic mechanical stretch. These physical signals are known to influence a variety of cell functions, including the secretion of growth factors and extracellular matrix proteins by cardiac fibroblasts, calcium handling and contractility in cardiomyocytes, or stretch-activated ion channels in muscle and non-muscle cells of the cardiovascular system. Recent progress in cardiac tissue engineering suggests that controlled physical stimulation can lead to functional improvements in multicellular cardiac tissue constructs. To study these effects, aspects of the physical environment of the myocardium have to be mimicked in vitro. Applying continuous live imaging, this protocol demonstrates how a specifically designed bioreactor system allows controlled exposure of cultured cells to cyclic stretch, rhythmic electrical stimulation, and controlled fluid perfusion, at user-defined temperatures.