Cyanotoxins in groundwater; occurrence, potential sources, health impacts and knowledge gap for public health.

Groundwater is a significant source of water across the world and constitutes about 30% of the earth's freshwater. This water source is likely to be contaminated by cyanobacteria that produce secondary metabolites called cyanotoxins. Studies on contamination of groundwater by cyanobacteria have been sketchy with limited information. There is a need for better evidence regarding groundwater contamination by cyanobacteria as their presence in surface water bodies could cause contamination of groundwater via infiltration and percolation during rainfall events or during groundwater-surface water interaction, bank infiltration or water quality exchange. Therefore, this review is aimed at exploring the occurrences and potential sources of cyanotoxins in groundwater. This was achieved by summarising the existing data on the occurrence of cyanobacteria in groundwater and their potential sources across the world. Groundwater cyanobacteria contamination can possibly pose threat to water quality because many of the cyanotoxins produced by cyanobacteria pose a severe threat to human health, animals and the environment. Concentrations of microcystins (MCs) in groundwater have been recorded in China (Chaohu), Saudi Arabia, and China (Huai River Basin), with concentrations of 1.446 µg/L, 1.8 µg/L and 1.07 µg/L, respectively. In humans, exposure to these cyanotoxins can cause symptoms such as vomiting, diarrhea, and skin irritation, to mention a few. This work highlights the importance of providing information or knowledge regarding public health implications of exposure to groundwater contaminated with cyanotoxins and the need to undertake risk management actions through national and international regulation. This review also points out current knowledge gaps, which could lead to future research.

Determinants of Solid Fuel Use and Emission Risks among Households: Insights from Limpopo, South Africa.

Emissions from residential solid fuels reduce ambient air quality and cause indoor air pollution resulting in adverse human health. The traditional solid fuels used for cooking include coal, straws, dung, and wood, with the latter identified as the prevalent energy source in developing countries. Emissions from such fuel sources appear to be significant hazards and risk factors for asthma and other respiratory diseases. This study aimed at reporting factors influencing the choice of dominant solid fuel for cooking and determine the emission risk from such solid fuel in three villages of Phalaborwa, Limpopo province, South Africa. The study used descriptive analysis to show the relationship between the socio-economic variables and the choice of cooking fuel at the household level. Multiple correspondence analysis (MCA) was used further to detect and represent underlying structures in the choice of dominant fuels. MCA shows the diversity and existing relationship of how variables are related analytically and graphically. Generalised linear logistic weight estimation procedure (WLS) was also used to investigate the factors influencing choice of fuel used and the inherent emission risks. In the three villages, wood was the prevalent cooking fuel with 76.8% of participant households using it during the summer and winter seasons. Variables such as low monthly income, level of education, and system of burning are revealed as strong predictors of wood fuel usage. Moreover, income, water heating energy, types of wood, and number of cooking hours are significant (p ≤ 0.05) in influencing emission from wood fuel in the community. A notable conclusion is that variables such as income, education status and system of burning are determinants of wood fuel usage in the three villages, while income, water heating energy, types of wood and number of hours influence vulnerability to household emission and possible health risks in the use of solid energy sources.

Occupational Health Risk of Waste Pickers: A Case Study of Northern Region of South Africa.

In South Africa, waste pickers play a significant role in the management of waste at landfill sites. Waste picking is an income-generating venture for most people with low-income base. The activity of sorting waste at landfill sites is, however, associated with occupational health risks to waste pickers which this study has examined. The study adopted a cross-sectional survey with a convenience sampling method which was conducted among 114 waste pickers in three landfill sites in Limpopo Province of South Africa. A validated questionnaire was used in eliciting responses from the participants. The statistical technique employed includes the ANOVA, simple, and multiple regression. The results indicated that, in the last one year, waste pickers exposed to landfill sites were 1.7 times more likely to develop a common health disorder (AOR: 1.733; 95% CI: 1.069, 2.755; P value: 0.041). There was statistical significance between the number of days worked at the landfill and the health conditions of the waste pickers (P ≤ 0.001). The cofounders were adjusted for age and years worked, and the result revealed that days worked by the waste pickers' increased their chances of occupational health risks by 1.4 times. It is unlikely that waste pickers will have a risk-free environment, but supportive policies such as provision of adequate personal protective equipment and more awareness programmes on the health risks related to such enterprises will aid in abating the associated risks.

Geochemical characterization and assessment of fluoride sources in groundwater of Siloam area, Limpopo Province, South Africa.

Siloam's groundwater is reportedly characterized by high fluoride. In response to the reported high incidence of dental fluorosis in the area, sources of elevated fluoride in the groundwater were investigated. Total fluoride (TF) was determined using Ion Chromatograph and Fluoride Ion Selective Electrode. The mineral composition of rocks and soils were determined using X-ray Fluorescence and X-ray diffraction, respectively. Results revealed that groundwater fluoride concentration ranged from 3.92 to 4.95 mg/L. Na-Cl water type was found to be dominant in the water samples. TF content of the rocks and soils ranged from 10 to 2000 mg/L. Leachates were obtained by making a slurry from the samples at a predetermined temperature and time. TF in leachates ranged between 0.27 and 14.88 mg/L and 0.05 to 10.40 mg/L at induced, and non-induced temperatures, respectively. The possible source of fluoride has been previously inferred to be caused by fluorite minerals occurring at greater depth. However, this study proves that fluoride decreases with depth and the elevated fluoride in the groundwater is caused by smectite-kaolinite clay, muscovite and chlorite minerals abundant in the area. Geothermal temperature exhibited by the groundwater in the area is a major factor enhancing the release of fluoride from the clay materials.

Water Quality Assessment and Evaluation of Human Health Risk in Mutangwi River, Limpopo Province, South Africa.

Freshwater supply is essential to life on Earth; however, land use activities such as mining and agriculture pose a significant danger to freshwater resources and the wellbeing of aquatic environments. This study temporarily assesses the water quality characteristics of Mutangwi River. Physicochemical parameters (pH, temperature, total dissolved solids (TDS), salinity, electrical conductivity (EC), and turbidity) were determined in situ using an Extech multimeter and turbidity meter. The concentration of the selected metals (Mg, Cr, Fe, Cd, Mn, Pb, Ca, and Na) were analysed using an Atomic Absorption Spectrophotometer. Membrane filtration method was used to analyse microbiological parameters (Escherichia coli and Enterococci). The physicochemical water quality parameters as well as basic anions (fluoride, phosphate, sulfate, nitrate, and chloride) determined complied with the regulatory guideline of the World Health Organization (WHO) and the South Africa National Standards (SANS). Some of the trace metals (Mn, Ca, Fe, and Mg) were found below the guideline values, while others (Pb and Cd) exceeded the threshold limit. The counts for E. coli (814.5-2169 cfu/100 mL) and Enterococci (333-9396 cfu/100 mL) in the study did not comply with the regulatory guidelines. The water quality status using the water quality index (WQI) indicated that on the average, the water quality from Mutangwi River is poor (WQI > 100). The hazard quotient through ingestion exposure did not exceed the threshold limit of 1, for adults and children. This implies that there is no potential non-carcinogenic health risk from trace elements via ingestion of drinking water for children and adults. However, cancer risk for adults and children was computed in relation to Cd and Pb levels and exceeded the threshold limit 10-4, indicating a possible carcinogenic risk. Water from the river should be adequately treated prior to domestic and agricultural use.

Investigating the chromium status, heavy metal contamination, and ecological risk assessment via tannery waste disposal in sub-Saharan Africa (Kenya and South Africa).

To mitigate the negative environmental and public health concerns associated with increased industrial discharges amid the rapid industrial growth, sub-Saharan African countries have instituted several environmental regulations and policies. However, a paradox of good environmental policies but inadequate enforcement exists in most of these countries, with information on the pollution level unknown due to poor monitoring resources. In this study, potentially toxic heavy metal contamination and physicochemical characteristics in effluents and dumpsite soils of two tanneries in Kenya (DB) and South Africa (BO) were evaluated during the dry and rainy season of 2018. Pollution levels and ecological risk in the dumpsite soils were assessed by adopting geoaccumulation index (Igeo), contamination factor (CF), pollution load index (PLI), and ecological risk index (PRI). The results showed that the mean final effluent concentration for total dissolved solids (TDS), chemical oxygen demand (COD), and Cr, Cu, Fe, Ni, Zn, and Cd for BO (2127, 890, 1.82, 1.38, 1.96, 0.60, 1.21, and 1.16, respectively) and DB tanneries (8157, 1369, 7.90, 0.69, 1.05, 0.60, 1.72 mg/L, respectively) were above the limits of emission guidelines. The mean Cr and Cr(VI) concentrations in tannery dumpsite soils (204.9 ± 29.1 and 0.31 ± 0.01 and 943 ± 29.8 and 0.4 ± 0.07 mg/kg for BO and DB, respectively) and Fe (2498 ± 62 mg/kg in DB) exceeded acceptable thresholds of the World Health Organization (WHO), Food and Agriculture Organization (FAO), and local background levels. A positive strong correlation was observed between Cr and organic matter, OM (r > 0.7, p < 0.001), electrical conductivity, EC (r = 0.99, p < 0.05), and As (r = 0.62; p < 0.05), suggesting a common anthropogenic point source. The mean PLI values of 5.3 and 1.6 for DB and BO dumpsites indicated significant pollution of the soils with heavy metals, specifically Cr (Igeo = 18 and 2.4 for DB and BO, respectively). Similarly, PRI values of 174.8 and 57.4 indicated a moderate and low potential ecological risk for DB and BO tannery dumpsite, respectively, with several plants sampled within the two sites exhibiting elevated levels of Cr contamination. In summary, these results provide scientific insights on the need for both improved effluent management and treatment technologies of tannery wastes, coupled with the strengthening of continuous monitoring and enforcement for compliance of industrial discharges in sub-Saharan countries.

Assessment of selected trace metals in fish feeds, pond water and edible muscles of Oreochromis mossambicus and the evaluation of human health risk associated with its consumption in Vhembe district of Limpopo Province, South Africa.

Fish is a rich source of proteins for humans and is widely consumed in various places in the world. This study assessed the levels of twenty trace metals (B, Al, V, Cr, Mn, Fe, Co, Ni, Cu, Zn, As, Se, Sr, Mo, Cd, Sn, Sb, Ba, Hg and Pb) in fish feeds (n = 2), water (n = 27), and edible muscles of Oreochromis mossambicus (n = 20 from 8 ponds) from fish farms and Luvuvhu River (n = 3 from 1 river site) in Vhembe district of Limpopo Province, South Africa. Physicochemical parameters of water in the study area were analysed. Temperature ranged between 21.4-30.47 °C, pH: 5.59-7.28, electrical conductivity: 608-1216 µS/cm, total dissolved solids: 156-675 mg/l, dissolved oxygen: 0.28 - 0.56 mg/l, turbidity: 3.92-356.7 NTU, respectively. Levels of most trace metals such as Cr (2 µg/l and 1000 µg/kg), Mn (100 µg/l and 500 µg/kg), Fe (10 µg/l and 300 µg/kg), Ni (20 µg/l and 100 µg/kg), As (50 µg/l and 3 µg/kg), Pb (10 µg/l and 300 µg/kg) and Cu (2 µg/l and 2250 µg/kg) in water and O. mossambicus muscles were mostly below the World Health Organization (WHO), Food and Agricultural Organization (FAO)/WHO and European Commission (EC) recommended limit in the collected samples (Tshifulalani site and Duthuni site). However, Cu and Fe recorded concentration above the recommended limit at the control site (Luvuvhu River) in water and O. mossambicus muscles, respectively. Results from the bioaccumulation factor (BF), suggests the presence of metals in the water which can bioaccumulate in the fish muscles. Most of the metals (As, Cd, Hg, Mo, Sr, Sb and Sn) that recorded no possible bioaccumulation also recorded levels that complied with their maximum permissible limit (MPL) of WHO, FAO/WHO and EC guideline values in the fish feeds except for Se and Co. Moderate bioaccumulation was recorded for Cr, Mn, Fe and Zn in some of the sampling sites. Pb and Ni showed extreme bioaccumulation (BF > 5000) in one of the sampling sites and also recorded elevated levels in the fish feeds. Average daily dose (ADD), hazard quotient (HQ) and total hazard quotient (THQ) computed were less than 1. The Cancer risk (CR) evaluated were all below 10-4 except in one site for children (Tshifulanani 2) but the overall average result showed no carcinogenic health risk to the consumers of the O. mossambicus. Therefore, O. mossambicus intake in the study area should be constantly monitored to prevent future health implications.

Evaluation of contaminants removal by waste stabilization ponds: A case study of Siloam WSPs in Vhembe District, South Africa.

Waste stabilization ponds (WSPs) are widely used for wastewater management owing to the simplicity of their design, low cost and the use of low-skilled operators. This study was carried out to assess the efficiency of a WSP system in reducing the levels of contaminants in hospital wastewater in a rural area of South Africa and to evaluate the current management of the WSP system. Sampling was conducted monthly from January to June 2014. Physicochemical and microbiological parameters were monitored using standard methods. The microbiological parameters (Escherichia coli and enterococci) in the effluent were higher than those in the influent in some sampling months. Also, low pathogen removal efficiency (<1 log reduction) was recorded. The chemical oxygen demand (COD) in the effluent (82-200 mg/L) exceeded the South African Department of Water Affairs for wastewater discharge guideline value of 75 mg/L although reduction efficiencies of 7.7%, 49.1% and 31.1% were observed for the months of February, April and June, respectively. The WSP system did not show a general trend of contaminant reduction except for Zn (5.5-94.8%). The Siloam WSP is not functioning properly and is releasing effluent of poor quality into the receiving river. It is recommended that the WSP system be expanded to cater for the extra load of wastewater it receives, also desludging should be performed as recommended for such systems. Continuous monitoring of the system for compliance to regulatory guideline should be routinely performed.

Impact of Low-Cost Point-of-Use Water Treatment Technologies on Enteric Infections and Growth among Children in Limpopo, South Africa.

Enteric infections early in life have been associated with poor linear growth among children in low-resource settings. Point-of-use water treatment technologies provide effective and low-cost solutions to reduce exposure to enteropathogens from drinking water, but it is unknown whether the use of these technologies translates to improvements in child growth. We conducted a community-based randomized controlled trial of two water treatment technologies to estimate their effects on child growth in Limpopo, South Africa. We randomized 404 households with a child younger than 3 years to receive a silver-impregnated ceramic water filter, a silver-impregnated ceramic tablet, a safe-storage water container alone, or no intervention, and these households were followed up quarterly for 2 years. We estimated the effects of the interventions on linear and ponderal growth, enteric infections assessed by quantitative molecular diagnostics, and diarrhea prevalence. The silver-impregnated ceramic water filters and tablets consistently achieved approximately 1.2 and 3 log reductions, respectively, in total coliform bacteria in drinking water samples. However, the filters and tablets were not associated with differences in height (height-for-age z-score differences compared with no intervention: 0.06, 95% CI: -0.29, 0.40, and 0.00, 95% CI: -0.35, 0.35, respectively). There were also no effects of the interventions on weight, diarrhea prevalence, or enteric infections. Despite their effectiveness in treating drinking water, the use of the silver-impregnated ceramic water filters and tablets did not reduce enteric infections or improve child growth. More transformative water, sanitation, and hygiene interventions that better prevent enteric infections are likely needed to improve long-term child growth outcomes.

The Equilibrium, Kinetics, and Thermodynamics Studies of the Sorption of Methylene Blue from Aqueous Solution Using Pulverized Raw Macadamia Nut Shells.

In this study, the potential for pulverized raw macadamia nut shell (MNS) for the sequestration of methylene blue from aqueous media was assessed. The sorbent was characterized using scanning electron microscopy for surface morphology, functional group analysis was performed with a Fourier-transform infrared spectrometer (FT-IR), and Brunauer-Emmett-Teller (BET) isotherm was used for surface area elucidation. The effects of contact time, sorbent dosage, particle size, pH, and change in a solution matrix were studied. Equilibrium data were fitted using Temkin, Langmuir, and Freundlich adsorption isotherm models. The sorption kinetics was studied using the Lagergren pseudo-first-order, pseudo-second-order, Elovich, and intraparticle diffusion models. The feasibility of the study was established from the thermodynamic studies. A surface area of 2.763 m2/g was obtained. The equilibrium and kinetics of sorption was best described by the Langmuir and the pseudo-second-order models, respectively. The sorption process was spontaneous (-ΔG 0=28.72 - 31.77 kJ/mol) and endothermic in nature (ΔH 0=17.45 kJ/mol). The positive value of ΔS0 (0.15 kJ/molK) implies increased randomness of the sorbate molecules at the surface of the sorbent. This study presents sustainable management of wastewater using MNS as a potential low-cost sorbent for dye decontamination from aqueous solution.

A review on the potential sources and health implications of fluoride in groundwater of Sub-Saharan Africa.

Groundwater is a major source of drinking water for millions of people around the world. Over 400 million people in Africa depend solely on it as their main source of water supply. Fluoride is a common contaminant in groundwater. In low concentration (0.5-1.0 mg/L), fluoride is needed by humans for healthy development of bones and teeth, however, a concentration >1.5 mg/L has been linked with several fluorosis and non-fluorosis diseases. Dental and skeletal fluorosis are the major fluorosis diseases commonly reported with the consumption of fluoride-rich water. Although fluoride intake through other pathways such as the drinking of tea and eating of vegetables have been reported, the drinking of fluoride-rich water remains the major pathway of fluoride into humans. Cases of high fluoride levels in groundwater have been reported in almost all the sub-Saharan Africa region but it is more prevalent in East African countries, Sudan and South Africa. Although fluoride is present in surface water mostly in the East African Rift Valley across different countries in East Africa, its significant or high levels are usually associated with groundwater. Geogenic sources such as fluorite, apatite, biotite, amphibole, micas, topaz, cryolite, muscovite and fluorspar have been identified as the major sources of fluoride in groundwater. High fluoride levels have been reported across sub Saharan Africa, with generally higher levels in East Africa resulting from the volcanic activities in the rift system. Dental fluorosis has been reported in many sub-Saharan African countries including South Africa, Tanzania, Uganda, Ethiopia, Kenya, Sudan, Niger, Nigeria, Benin, Ghana and Malawi. Geothermal temperature has been regarded as one of the driving forces for high fluoride levels recorded in groundwater from deep aquifers and geothermal springs. The most affected people with the consumption of fluoride-rich water are the poor with low socioeconomic status who live in rural areas. Some of the proposed alternative sources include rainwater and fog water harvesting and blending of water from various sources. Low-cost and sustainable deflouridation technique remains one of the best ways to treat fluoride contaminated water either at communal level or at the point-of-use.

The Potential Health Risk Associated with Edible Vegetables Grown on Cr(VI) Polluted Soils.

This study reports on the assessment of the growth potential of five edible vegetables, which were grown in Cr(VI) spiked soils. The vegetable plants that were used in this study were Vigna angularis, Cicer arietinum, Spinacia oleracea, Amaranthus dubius Thell and Phaseolus vulgaris. Dried ground samples from roots, stems and leaves were analysed for various oxidation states of Cr. The daily intake of chromium, hazard quotient (HQ) and hazard index (HI) methods were employed to assess the potential human health risks posed by these Cr oxidation states through vegetable consumption. The results showed that Vigna angularis was the only vegetable that germinated in highly concentrated Cr(VI) in the simulated soil (456 mg/kg). The highest total chromium (ChT) bioaccumulated in the roots was found in Phaseolus vulgaris at 0.8. The highest ChT translocation factor in the stem was that of Cicer arietinum and Vigna angularis at 0.30. The same plants translocated the highest ChT to the leaf at 0.7. A child or an adult consuming such contaminated Cicer arietinum vegetables were likely to take in between 508 and 785 mg/day of ChT, which are above the World Health Organisation guidelines of 220 and 340 mg/day, respectively. The highest HQ was found in Cicer arietinum at 8.7 and 13.4 for adults and children, respectively. The same species of plants also had high HI at 17.4 and 27.2 for adults and children, respectively. This indicated that consumers of the edible vegetables grown in Cr(VI) rich soils may be exposed to health risks, and the children were more likely to be vulnerable to these adverse effects than the adults.

Influence of Selective Conditions on Various Composite Sorbents for Enhanced Removal of Copper (II) Ions from Aqueous Environments.

Numerous pollutants, including dyes, heavy metals, pesticides, and microorganisms, are found in wastewater and have great consequences when discharged onto natural freshwater sources. Heavy metals are predominantly reported in wastewater. Heavy metals are persistent, non-biodegradable and toxic, transforming from a less toxic form to more toxic forms in environmental media under favourable conditions. Among heavy metals, copper is dominantly found in wastewater effluent. In this review, the effects of high concentration of copper in plants and living tissues of both aquatic animals and humans are identified. The performance of different polymer adsorbents and the established optimum conditions to assess the resultant remediation effect as well as the amount of copper removed are presented. This procedure allows the establishment of a valid conclusion of reduced time and improved Cu (II) ion removal in association with recent nano-polymer adsorbents. Nano-polymer composites are therefore seen as good candidates for remediation of Cu ions while pH range 5-6 and room temperature were mostly reported for optimum performance. The optimum conditions reported can be applied for other metal remediation and development of potent novel adsorbents and process conditions.

Characterization of pulverized Marula seed husk and its potential for the sequestration of methylene blue from aqueous solution.

Dyes are ranked as an important class of pollutants which affect the aesthetic property of the environment when present even in very low concentrations. This study was carried out to explore the potential use of an agricultural waste (Marula seed husk) to decontaminate methylene blue (MB) from aqueous solution. The effect of change in water chemistry was also examined. The influence of basic adsorption parameters such as contact time, temperature, dosage, pH and particle size on the efficiency of adsorption were investigated. Langmuir and Freundlich isotherms were used to describe the equilibrium data while Pseudo first, second order and Elovich kinetic models were used to evaluate the kinetics of the adsorption process. Thermodynamic parameters such as change in enthalpy (ΔH°), entropy (ΔS°) and Gibbs free energy (ΔG°) were evaluated. Natural surface water showed higher MB removal efficiency than de-ionized water. The sorption process was favored more in alkaline pH range (7-10). The dye adsorption process was found to be endothermic, while ΔG° was negative implying that the reaction is spontaneous. Functional group analyses on the adsorbent showed the presence of hydroxyl, carbonyl and carboxyl groups. The Langmuir equilibrium model best described the adsorption process based on the linearized coefficient. The Pseudo second order model best described the kinetics of the reaction.

Health and Environmental Risks of Residents Living Close to a Landfill: A Case Study of Thohoyandou Landfill, Limpopo Province, South Africa.

The by-products of solid waste deposited in a landfill has adverse effects on the surrounding environment and humans living closer to landfill sites. This study sought to test the hypothesis that the deposition of waste on landfill has an impact on the surrounding environment and residents living closer to it. This was achieved by evaluating the perception of the respondents drawn from people living close (100-500 m) and far (1-2 km) from the landfill site, concerning environmental issues, health problems, and life satisfaction. Results from the study showed that 78% of participants living closer to the landfill site indicated serious contamination of air quality evident from bad odours linked to the landfill site. Illnesses such as flu, eye irritation and weakness of the body were frequently reported by participants living closer to the landfill than those living far from the landfill. More than half of the participants (56%) living closer to the landfill indicated fear of their health in the future. Thus, the participants living closer to the landfill site were less satisfied with the location of their community with respect to the landfill, than those living far from the landfill site. Therefore, the need for a landfill gas (LFG) utilisation system, proper daily covering of waste and odour diluting agents are necessary to reduce the problems of the residents living closer to the landfill site.

Physico-Chemical Analysis of Wastewater Discharge from Selected Paint Industries in Lagos, Nigeria.

Effluents from the paint industry have been a major source of environmental pollution. There is a need to investigate the compliance of wastewater discharged from paint industries with regulatory standards. In response, this study evaluates the physicochemical parameters of both raw and treated wastewater, the wastewater treatment plants (WWTPs) efficiencies as well as the compliance level of five selected paint manufacturing companies in Lagos, Nigeria with some regulatory standards: Federal Ministry of Environment (FME) in Nigeria, World Health Organization (WHO) and Department of Water Affairs (DWA) in South Africa. All parameters investigated were analysed using standard methods. The values of pH, electrical conductivity (EC) and total dissolved solids (TDS) levels were in the range of 4⁻12.2, 149.1⁻881.3 mS/m and 1100⁻6510 mg/L, respectively. The range of other parameters include total suspended solids (TSS); 0⁻2470 mg/L, TS; 1920⁻6510 mg/L, chloride; 63.8⁻733.8 mg/L, dissolved oxygen (DO); 0⁻6.7 mg/L, oil and grease (O & G); 44⁻100 mg/L, biochemical oxygen demand (BOD); 162.8⁻974.7 mg/L, chemical oxygen demand (COD); 543⁻1231 mg/L, nitrates;12.89⁻211.2 mg/L, phosphate; below detection limit (bdl)⁻0.02 mg/L, sulphate; 195⁻1434 mg/L, nickel; bdl⁻1.9 mg/L while copper, lead and chromium were below detection limits. The results indicated that the WWTPs of the studied paint companies were ineffective in reducing the TS, TSS, BOD, COD and (O & G) to acceptable limits. Routine monitoring of wastewater from paint industries is therefore recommended to prevent the risk of contamination to the receiving watershed which many communities rely on as source for domestic water.

Evaluation of Microbiological and Physicochemical Parameters of Alternative Source of Drinking Water: A Case Study of Nzhelele River, South Africa.

BACKGROUND: Access to clean and safe drinking water is still a problem in developing countries and more pronounced in rural areas. Due to erratic supply of potable, rural dwellers often seek for an alternative source of water to meet their basic water needs. The objective of this study is to monitor the microbiological and physicochemical water quality parameters of Nzhelele River which is a major alternative source of drinking water to villages along its course in Limpopo province of South Africa. METHODS: Membrane filtration method was employed in evaluating the levels of E. coli and Enterococci in the river water from January-June, 2014. Specialized multimeter was used to measure the pH, electrical conductivity and turbidity of the river water. Ion Chromatograph was used to measure major anions such as fluoride, chloride, nitrate and sulphate in the water. RESULTS: High levels of E. coli (1 x 102 - 8 x 104 cfu/100 mL) and enterococci (1 x 102 - 5.7 x 103 cfu/100 mL) were found in the river water and exceeded their permissible limits of 0 cfu/100 mL for drinking water. Turbidity values ranged from 1.12-739.9 NTU. The pH, electrical conductivity, chloride, fluoride, nitrate and sulphate levels were below their permissible limits for drinking water. CONCLUSION: The river water is contaminated with faecal organisms and is unfit for drinking purposes. However, the levels of the major anions accessed were within the permissible limits of drinking water.

Challenges to Sustainable Safe Drinking Water: A Case Study of Water Quality and Use across Seasons in Rural Communities in Limpopo Province, South Africa.

Consumption of microbial-contaminated water can result in diarrheal illnesses and enteropathy with the heaviest impact upon children below the age of five. We aimed to provide a comprehensive analysis of water quality in a low-resource setting in Limpopo province, South Africa. Surveys were conducted in 405 households in rural communities of Limpopo province to determine their water-use practices, perceptions of water quality, and household water-treatment methods. Drinking water samples were tested from households for microbiological contamination. Water from potential natural sources were tested for physicochemical and microbiological quality in the dry and wet seasons. Most households had their primary water source piped into their yard or used an intermittent public tap. Approximately one third of caregivers perceived that they could get sick from drinking water. All natural water sources tested positive for fecal contamination at some point during each season. The treated municipal supply never tested positive for fecal contamination; however, the treated system does not reach all residents in the valley; furthermore, frequent shutdowns of the treatment systems and intermittent distribution make the treated water unreliable. The increased water quantity in the wet season correlates with increased treated water from municipal taps and a decrease in the average contaminant levels in household water. This research suggests that wet season increases in water quantity result in more treated water in the region and that is reflected in residents' water-use practices.

Determination and Distribution of Polycyclic Aromatic Hydrocarbons in Rivers, Sediments and Wastewater Effluents in Vhembe District, South Africa.

Polycyclic aromatic hydrocarbons are very toxic and persistent environmental contaminants. This study was undertaken to assess the concentrations and possible sources of 16 PAHs (Polycyclic aromatic hydrocarbons) classified by the United State Environmental Protection Agency as priority pollutants in water and sediments of the Mvudi and Nzhelele Rivers. Effluents from Thohoyandou wastewater treatment plant and Siloam waste stabilization ponds were also investigated. Diagnostic ratios were used to evaluate the possible sources of PAHs. PAHs in the water samples were extracted using 1:1 dichloromethane and n-hexane mixtures, while those in the sediment samples were extracted with 1:1 acetone and dichloromethane using an ultrasonication method. The extracts were purified using an SPE technique and reconstituted in n-hexane before analyses with a gas chromatograph time of flight-mass spectrometer. The results obtained indicate the prevalence of high molecular weight PAHs in all the samples. PAHs concentrations in water and sediment samples from all the sampling sites were in the range of 13.174-26.382 mg/L and 27.10-55.93 mg/kg, respectively. Combustion of biomass was identified as the major possible source of PAHs. Effluents from wastewater treatment facilities were also considered as major anthropogenic contributions to the levels of PAHs found in both river water and sediments. Mvudi and Nzhelele Rivers show moderate to high contamination level of PAHs.

Removal Efficiency of Faecal Indicator Organisms, Nutrients and Heavy Metals from a Peri-Urban Wastewater Treatment Plant in Thohoyandou, Limpopo Province, South Africa.

Wastewater treatment facilities are known sources of fresh water pollution. This study was carried out from January to June 2014 to assess the reduction efficiency of some selected contaminants in the Thohoyandou wastewater treatment plant (WWTP). The pH and electrical conductivity of the effluent fell within the South African wastewater discharge guidelines. The WWTP showed the chemical oxygen demand reduction efficiency required by the Department of Water Affairs (DWA) guidelines of 75 mg/L for the months of April and June, although it was below this standard in March and May. Free chlorine concentration varied between 0.26-0.96 mg/L and exceeded the DWA guideline value of 0.25 mg/L. The concentration of nitrate-nitrogen (NO3(-) N) in the influent and effluent varied between 0.499-2.31 mg/L and 7.545-19.413 mg/L, respectively. The concentration of NO3- N in the effluent complied with DWA effluent discharge standard of 15 mg/L, except in April and May. Phosphate concentrations in the influent and effluent were in the ranges of 0.552-42.646 mg/L and 1.572-32.554 mg/L, respectively. The WWTP showed reduction efficiencies of E. coli and Enterococci during some sampling periods but the level found in the effluent exceeded the recommended guideline value of 1000 cfu/100 mL for faecal indicator organisms in wastewater effluents. Consistent removal efficiencies were observed for Al (32-74%), Fe (7-32%) and Zn (24-94%) in most of the sampling months. In conclusion, the Thohoyandou WWTP is inefficient in treating wastewater to the acceptable quality before discharge.