RESUMO
Polymethylmethacrylate (PMMA) has been the most-widely used denture base material in prosthetic dentistry for the last 80 years. It is still one of the best alternatives when new methods are inapplicable. Due to the lack of some physical inadequacies occurring during cyclic use and accidental situations, various reinforcement strategies such as using nanoparticles, wires, fibers, and meshes have been investigated and reported. In this study, it was aimed to conduct a comparative investigation of the effect of fiber additives with different characteristics on the flexural properties of heat-cured PMMA denture base resins. Glass fibers (GFs), polypropylene fibers (PPFs), and carbon fibers (CFs) having 3, 6, and 12 mm lengths and 0.25, 0.50, and 1.0% concentrations (v/v) were used for the reinforcement of PMMA denture base resins. The flexural properties (flexural strength, flexural modulus, and maximum deformation) were determined using a three-point bending test, and three-way ANOVA analyses with Bonferroni corrections were performed on the test results. The morphologies of the fracture surfaces were analyzed using scanning electron microscopy. All three fibers exhibited reinforcement in the flexural strength (p < 0.001) and flexural modulus (p < 0.001) regardless of their length and concentration. The group with 1.0% 12 mm CF-reinforced PMMA exhibited the greatest flexural strength (94.8 ± 8.8 MPa), and that with 1.0% 3 mm GFs displayed the lowest flexural strength (66.9 ± 10.4 MPa) among the fiber-reinforced groups. The greatest value of the flexural modulus was displayed by the 1.0% 3 mm CF-reinforced resin (3288.3 ± 402.1 MPa). Although the CF-reinforced groups exhibited better flexural properties, CFs are not favorable for use as reinforcement in practice due to the dark gray discoloration of the denture base resin. It was concluded that PPF is a promising material for the reinforcement of heat-cured PMMA denture base resins.
RESUMO
The aim of this study was to investigate the potential use of polypropylene (PP) hernia mesh as a reinforcement of PMMA denture base resin in comparison with metal and glass fiber meshes, with the expectation of enhancing the mechanical stability of the PMMA dentures in oral conditions. The control group with no mesh, the aluminum metal mesh (Al) group, the PP1 (PP mesh used on top) group, the PP2 (PP mesh used on both the top and bottom) group, the orthopedic casting tape with self-curing resin (DP0) group, and the flushed form (DPA) group were fabricated in a chromium mold. A total of 144 specimens were divided into three equal portions and subjected to: first, no treatment; second, thermal cycling only; and third, thermal cycling and chewing simulation. The flexural strength, maximum deformation, and flexural modulus were determined by a three-point bending test to compare mechanical properties. Fracture surfaces were evaluated by scanning electron microscopy. The obtained data were statistically analyzed by a two-way ANOVA test with Bonferroni corrections. The non-treated Al mesh reinforcement group exhibited the highest (82.66 ± 6.65 MPa) flexural strength, and the PP2 group treated with chewing simulation displayed the lowest (56.64 ± 4.59 MPa) flexural strength. The Al group showed the highest (7.25 ± 1.05 mm) maximum deformation and the PP2 group showed lowest (3.64 ± 0.28 mm) maximum deformation when both groups were not subjected to any treatment. The control group with no treatment exhibited the lowest (1556.98 ± 270.62 MPa) flexural modulus values, and the Al group with no treatment exhibited the highest (3106.07 ± 588.68 MPa) flexural modulus values. All the mesh groups displayed intact fractures. Any type of mesh used for reinforcement exhibited a significant change in all flexural properties (p < 0.001). The PP1 reinforcement group did not exhibit a significant change in mechanical properties when the effect of treatment was compared. Using PP hernia mesh on top enhanced the mechanical properties despite the weakening when it was used on both the top and bottom. The mechanical stability provided by the PP hernia mesh indicated it to be a promising candidate to be used for reinforcement.
RESUMO
This study aims to describe a simple and environmentally friendly procedure for producing silver nanoparticles (AgNPs) using Paeonia kesrouanensis (P. kesrouanensis) extracts and to determine the toxic effect in the aquatic environment. The morphologies, size, size distributions, and structural properties were analyzed using SEM-EDX, TEM, DLS, zeta potential, FTIR, and XRD. AgNPs were applied to Artemia salina (A.salina), aquatic organism individuals at 7 different concentrations (0.0, 0.2, 1, 5, 10, 25, 50 mg/L) for 24, 48, and 72 h. AgNPs accumulation and elimination, ion release amounts, and the survival rates of organisms were determined at periods of 24, 48, and 72nd hours. The highest accumulation was observed at the 24th hour at the 50 mg/L exposure level. The survival rate decreased as exposure time increased at all concentrations.
Assuntos
Nanopartículas Metálicas , Paeonia , Humanos , Animais , Artemia , Prata/toxicidade , Prata/química , Nanopartículas Metálicas/toxicidade , Nanopartículas Metálicas/química , Extratos Vegetais/químicaRESUMO
Biodegradable PLA-PEG-PLA block copolymers were synthesized with desired backbone structures and molecular weights using PEG20000. Rectangular scaffolds were prepared by freeze drying with or without using NaCl particles. Bone morphogenetic protein (BMP)-2 was loaded to the matrix after the scaffold formation for sustained release while vascular endothelial growth factor (VEGF) was loaded within the pores with gelatin solution. VEGF release was quite fast and almost 60% of it was released in 2 d. However, sequential - sustained released was observed for BMP-2 in the following few months. Corporation of VEGF/BMP-2 couple into the scaffolds increased the cell adhesion and proliferation. Neither significant cytotoxicity nor apoptosis/necrosis were observed.
Assuntos
Osteoblastos/metabolismo , Poliésteres/química , Polietilenoglicóis/química , Engenharia Tecidual , Alicerces Teciduais/química , Fator A de Crescimento do Endotélio Vascular , Animais , Proteína Morfogenética Óssea 2/química , Proteína Morfogenética Óssea 2/farmacocinética , Proteína Morfogenética Óssea 2/farmacologia , Linhagem Celular , Preparações de Ação Retardada/química , Preparações de Ação Retardada/farmacocinética , Preparações de Ação Retardada/farmacologia , Humanos , Camundongos , Osteoblastos/citologia , Fator A de Crescimento do Endotélio Vascular/química , Fator A de Crescimento do Endotélio Vascular/farmacocinética , Fator A de Crescimento do Endotélio Vascular/farmacologiaRESUMO
In order to increase stabilities and controlled/sustained released of T4 phages were encapsulated within alginate beads which were then coated with chitosan, polyethylene imine (PEI). Quite high loading capacities (over 90%) were achieved in these pH-sensitive microbeads. Coating with those polycations increased significantly stability both in "simulated gastric fluid" and bile salts especially in the case of PEI coating. The tests conducted in "simulated intestinal fluid" demonstrated that phages were released from the beads which were active at basic pH in which the release rates were smaller in case of chitosan. PEI concluded to be a better coating then chitosan.
Assuntos
Alginatos/química , Bacteriófago T4/química , Quitosana/química , Polietilenoimina/química , Ácido Glucurônico/química , Ácidos Hexurônicos/química , Concentração de Íons de HidrogênioRESUMO
PURPOSE: Promising developments have materialized in reconstructive surgical procedures with the applications of tissue engineering. In our study, we used tissue scaffolds fabricated from polylactic acid-polyethylene glycol (PLLA-PEG) copolymers to ensure different release rates of selective growth factors recombinant human bone morphogenetic protein 2 [rhBMP-2] and vascular endothelial growth factor (rhVEGF165) in the repair of mandibular bone defects. MATERIALS AND METHODS: In our experimental study, 54 New Zealand rabbits were used. The rabbits were separated into 4 groups: group I (control group), PLLA-PEG scaffold only; group II, PLLA-PEG scaffold plus rhBMP-2 application; group III, PLLA-PEG scaffold plus VEGF165 application; and group IV, PLLA-PEG scaffold plus rhBMP-2 and VEGF165 applications. The rabbits were killed at 4 and 8 weeks postoperatively, and histopathologic and immunohistochemical assessments were performed. RESULTS: The greatest bone volume was observed in rhBMP-2-containing groups, the greatest vessel volume was observed in VEGF165-containing groups; however, the scaffold containing rhBMP-2 and VEGF165 provided the best outcomes in conjunction with increased remodeling of the new bone. CONCLUSIONS: The use of polymer tissue scaffolds that release rhVEGF165 and rhBMP-2 in coordination and mimic the natural healing process in the regeneration of especially complex tissues, such as bone, is a promising treatment alternative in the field of reconstructive surgery.
Assuntos
Proteína Morfogenética Óssea 2/uso terapêutico , Mandíbula/cirurgia , Reconstrução Mandibular/métodos , Alicerces Teciduais , Fator A de Crescimento do Endotélio Vascular/uso terapêutico , Implantes Absorvíveis , Animais , Proteína Morfogenética Óssea 2/administração & dosagem , Osteogênese/efeitos dos fármacos , Coelhos , Fator A de Crescimento do Endotélio Vascular/administração & dosagemRESUMO
In this work, poly(L-lactic acid)-g-starch layered silicate nanocomposites (NCs) (PLLA-g-starch/MMT) were fabricated by intercalative bulk graft copolymerization of LA with starch, in the presence of either stannous octoate acting as a catalyst or LA-MMT organoclay acting as a cocatalyst-nanofiller. This procedure was performed inside a custom vacuum micro-reactor. To better understand the graft copolymerization mechanism, in situ processing types, interfacial interactions and nanostructure formation of PLLA-g-starch/MMT NCs, methods such as FT-IR, XRD, (1)H NMR, (13)C CP/MAS-NMR, DSC/TGA, TEM and SEM were utilized. The morphology and thermal behaviors of nanocomposites were found to be strongly dependent on the loading mass fraction of LA-MMT within the nanocomposite structure and the type of in situ processing such as interfacial, physical and chemical interactions. Preintercalated LA-MMT organoclay exhibited dual functions. It demonstrated the ability to act as a catalyst, essentially accelerating in situ graft copolymerization via esterification of LA carboxyl groups with hydroxyl groups of starch macromolecules, whilst also acting as a nananofiller-compatibilizer.
Assuntos
Bentonita/química , Ácido Láctico/química , Nanocompostos/química , Polímeros/química , Amido/química , PoliésteresRESUMO
The aim of this study was to investigate the effectiveness of a novel hydroxyapatite containing gelatin scaffold--with and without local vascular endothelial growth factor (VEGF) administration--as the synthetic graft material in treatment of critical-sized bone defects. An experimental nonunion model was established by creating critical-sized (10 mm. in length) bone defects in the proximal tibiae of 30 skeletally mature New Zealand white rabbits. Following tibial intramedullary fixation, the rabbits were grouped into three: The defects were left empty in the first (control) group, the defects were grafted with synthetic scaffolds in the second group, and synthetic scaffolds loaded with VEGF were administered at bone defects in the third group. Five rabbits in each group were killed on 6th and 12th weeks, and new bone growth was assessed radiologically, histologically and with dual-energy X-ray absorptiometry (DEXA). At 6 weeks, VEGF-administered group had significantly better scores than the other two groups. The second group also had significantly better scores than the control group. At 12 weeks, while no significant difference was noted between the second and third groups, these two groups both had significantly better scores in all criteria compared with the control group. There were no signs of complete fracture healing in the control group. The administration of hydroxyapatite containing gelatin scaffold yielded favorable results in grafting the critical-sized bone defects in this experimental model. The local administration of VEGF on the graft had a positive effect in the early phase of fracture healing.
Assuntos
Materiais Biocompatíveis/farmacologia , Durapatita/farmacologia , Fraturas não Consolidadas/fisiopatologia , Fraturas da Tíbia/fisiopatologia , Fator A de Crescimento do Endotélio Vascular/farmacologia , Análise de Variância , Animais , Densidade Óssea/fisiologia , Transplante Ósseo/métodos , Criogéis/farmacologia , Modelos Animais de Doenças , Fixação de Fratura/métodos , Consolidação da Fratura/efeitos dos fármacos , Fraturas não Consolidadas/patologia , Gelatina/farmacologia , Coelhos , Fraturas da Tíbia/patologia , Alicerces TeciduaisRESUMO
Biomaterials are widely used in diverse applications as substances, materials or important elements of biomedical devices. Biodegradable polymers, both natural and synthetic, have been utilized in applications in which they act as temporary substitutes. Poly(alpha-hydroxy acids), especially lactic acids and glycolic acid and their copolymers with epsilon-caprolactone, are the most widely known and used among all biodegradable polymers. They degrade in vivo into safe end products mainly by hydrolysis in a few weeks to several months, depending on several factors, including molecular structure/morphology, average molecular weight, size and shape. They are processed into tailor-made materials for diverse applications, although mainly for soft and hard tissue repair. Electrospinning is a method of producing nanofibers and nonwoven matrices from their solutions and melts. Several factors affect fiber diameter and resulting nonwoven structures/morphologies. Recently, electrospun matrices made of lactic acids, glycolic acid and epsilon-caprolactone homo- and co-polymers have been attracting increasing attention for fabrication of novel materials for medical use. This review briefly describes poly(alpha-hydroxy acids) and the elecrospinning process, and gives some selected recent applications of electrospun matrices made from these polymers.