Assuntos
Toxina Adenilato Ciclase/imunologia , Adenilil Ciclases/imunologia , Antígenos de Bactérias/imunologia , Bordetella pertussis/imunologia , Células Dendríticas/imunologia , Toxina Adenilato Ciclase/genética , Adenilil Ciclases/genética , Adenilil Ciclases/metabolismo , Animais , Antígenos de Bactérias/genética , Bordetella pertussis/enzimologia , Bordetella pertussis/genética , Células Dendríticas/enzimologia , Vetores Genéticos , Antígenos de Histocompatibilidade Classe I/genética , Antígenos de Histocompatibilidade Classe I/imunologia , Humanos , Ativação Linfocitária , Vacina contra Coqueluche/imunologia , Linfócitos T Citotóxicos/imunologiaRESUMO
The effect of LCMV on CD4+ T lymphocytes was analyzed in C3HeB/FeJ mice after infection with the Docile strain of this virus. Our results indicated that LCMV triggers: i) an inhibition of Th2 lymphocyte differentiation induced by concomitant immunization with a nonviral protein antigen; ii) a depression of T helper-dependent antibody responses elicited by such an immunization; and iii) a CD4+ cell-mediated proliferation of spleen cells leading to increased interleukin-4 and interferon-gamma message expression and IgG2a-restricted total immunoglobulin secretion. Taken together, these results indicate that LCMV profoundly affects CD4+ cell-mediated immune responses in infected animals. Such modulations of T-helper functions may explain the preponderance of IgG2a in the antierythrocyte autoimmune response induced by the virus in C3HeB/FeJ mice.