Performance of microbial inoculation and tricalcium phosphate on nitrogen retention and conversion: Core microorganisms and enzyme activity during kitchen waste composting.

The substantial release of NH3 during composting leads to nitrogen (N) losses and poses environmental hazards. Additives can mitigate nitrogen loss by adsorbing NH3/NH4, adjusting pH, and enhancing nitrification, thereby improving compost quality. Herein, we assessed the effects of combining bacterial inoculants (BI) (1.5%) with tricalcium phosphate (CA) (2.5%) on N retention, organic N conversion, bacterial biomass, functional genes, network patterns, and enzyme activity during kitchen waste (KW) composting. Results revealed that adding of 1.5%/2.5% (BI + CA) significantly (p < 0.05) improved ecological parameters, including pH (7.82), electrical conductivity (3.49 mS/cm), and N retention during composting. The bacterial network properties of CA (265 node) and BI + CA (341 node) exhibited a substantial niche overlap compared to CK (210 node). Additionally, treatments increased organic N and total N (TN) content while reducing NH4+-N by 65.42% (CA) and 77.56% (BI + CA) compared to the control (33%). The treatments, particularly BI + CA, significantly (p < 0.05) increased amino acid N, hydrolyzable unknown N (HUN), and amide N, while amino sugar N decreased due to bacterial consumption. Network analysis revealed that the combination expanded the core bacterial nodes and edges involved in organic N transformation. Key genes facilitating nitrogen mediation included nitrate reductase (nasC and nirA), nitrogenase (nifK and nifD), and hydroxylamine oxidase (hao). The structural equation model suggested that combined application (CA) and microbial inoculants enhance enzyme activity and bacterial interactions during composting, thereby improving nitrogen conversion and increasing the nutrient content of compost products.

Structure-guided engineering of immunotherapies targeting TRBC1 and TRBC2 in T cell malignancies.

Peripheral T cell lymphomas are typically aggressive with a poor prognosis. Unlike other hematologic malignancies, the lack of target antigens to discriminate healthy from malignant cells limits the efficacy of immunotherapeutic approaches. The T cell receptor expresses one of two highly homologous chains [T cell receptor ß-chain constant (TRBC) domains 1 and 2] in a mutually exclusive manner, making it a promising target. Here we demonstrate specificity redirection by rational design using structure-guided computational biology to generate a TRBC2-specific antibody (KFN), complementing the antibody previously described by our laboratory with unique TRBC1 specificity (Jovi-1) in targeting broader spectrum of T cell malignancies clonally expressing either of the two chains. This permits generation of paired reagents (chimeric antigen receptor-T cells) specific for TRBC1 and TRBC2, with preclinical evidence to support their efficacy in T cell malignancies.

Trace Minerals in Laying Hen Diets and Their Effects on Egg Quality.

With the advancement in the egg industry sector, egg quality has assumed great significance in certain countries. Enhancements in the nutritional value of eggs may have direct affirmative consequences for daily nutrient intake and therefore for human health. Thus, affirmative improvement in egg quality boosts consumer preferences for eggs. Also, the improvement in eggshell quality can avoid the disposal of broken eggs and consequently economic losses. Therefore, poultry nutrition and mineral supplements have a significant impact on egg quality. Minerals are crucial in poultry feed for a number of biological processes, including catalytic, physiologic, and structural processes. For instance, they contribute to the biological processes necessary for forming and developing eggshells. To produce high-quality eggs for sale, diets must therefore contain the right amount of minerals. This review aims to highlight the role of both organic and inorganic minerals in improving egg quality, in addition to reviewing the interactions of mineral supplements with intestinal microbiota and subsequent effects on the egg quality.

The relationship between eggshell color, hatching traits, fertility, mortality, and some qualitative aspects of Japanese quail (Coturnix japonica) eggs.

Quail, one of the most important sources of meat and eggs, can aid in the reduction of the meat crisis if they are raised and cared for by small farmers. The current study investigated the impact of eggshell color variety on egg quality traits and hatching parameters of Japanese quail Coturnix japonica eggs. Therefore, 1,075 eggs were collected from female quails when they were 10-wk old. These eggs were distributed based on the color of their eggshells into 5 different classifications: eggshell with color type 1, very dark distributed brown spots on brown eggshell; eggshell with color type 2, small black spots on a white eggshell; eggshell with color type 3, widespread brown spots on brown eggshell; eggshell with color type 4, bubble egg, pin dotted on grayish brown color eggshell; and eggshell with color type 5, small brown spots on very clearly white eggshell. The characteristics of hatchability, internal and external egg quality, and the rate of embryonic death were then determined. The results showed that the percentage of fertility and commercial and scientific hatchability was greatly affected by eggshell color. There were also variations in the percentage of hatched chicks', early mortality rates, and late mortality rates based on eggshell color. According to the results of the current study, eggshell color has a significant impact on egg weight, egg width, and percentages of eggshell, yolk, and albumen but has no effect on shape index, egg length, or egg elongation. Based on the results, eggshells with color types 2 and 3 were recommended for use in hatching procedures due to their high levels of fertility (92.01 and 91.63%, respectively), scientific hatchability (82.92 and 83.93%, respectively), commercial hatchability (76.56 and 77.32%, respectively), and hatched chick (5.50 and 6.70, respectively). In addition, the late embryonic mortality rate was 0.00% for eggshells with color type 3. Therefore, the color of the eggshell can be employed as a key factor in guiding the eggs that are produced, whether they are going to be consumed at the table or used for hatching, to make breeding easier.

Efficacy of different multi-layer hermetic bags on the seed quality of the faba bean (Vicia faba L.) in outdoor storage condition.

Faba bean seeds' sustainability correlates with the initial quality of cultivated seeds. The duration of storage is a significant factor that can affect the quality retention of any crop seed. Additionally, the hermetic bags effectively influence the quality of crop seeds during the storage process. This study evaluated two faba bean cultivars, Nubaria 1 and Giza 716, after various storage periods of 6, 12, 18, and 24 months. Seeds stored in 3 and 7-layer hermetic bags have shown significantly improved bulk temperature after 12, 18, and 24 months (neither more than ≈ 30 °C nor less than ≈ 15 °C). However, the relative humidity (RH%) increased significantly in both bags and cultivars throughout the storage periods, reaching 61, 59.77% and 59.53, 57.53% at the end of the storage period for Nubaria 1 and Giza 716 inside the 3 and 7-layer bags, respectively. The seeds' germination % decreased significantly (20%) after 24 months at the 3-layers compared with 13.12% at the 7-layer bags for Nubaria 1 with superior germination% of Giza 716 under the same conditions where the decrease in the percentage of germination reached 15.56% and 8.86% reduction for both 3 and 7 layers, respectively. The seedling vigor index exhibited the same trend of germination % with better results of the 7-layer bags for both cultivars. The moisture content (MC%) was substantially elevated by 1% at the end of the storage for both cultivars and bags. After 12 months, beans considerably increased color deterioration, with a loss of 43.16 and 53.60% for Giza 716 and Nubaria 1 stored in 3-layer bags, respectively; however, 7-layer bags were always better than 3-layer bags (with a loss of 32.56 and 45.56%). Furthermore, storage in 7-layer hermetic bags triggered a significant decrease (14.94%) in the total phenolic (TPC) after 24 months for Giza 716 without a substantial difference with Nubaria 1. Additionally, the simulation showed that after 18 months and 24 months of storage, the Nubaria 1 seeds packed in the 7-layer hermetic bags produced total tannins (TTC) 39.1% and 42.5%, respectively, more than those packed in the 3-layer hermetic bags. That TTC had a negative correlation with the testa's darkness. Taken together, the faba bean seeds could be stored for a long period in the 7-layer hermetic bags, preserving seed quality and expanding marketing prospects.

Development and evaluation of a hybrid smart solar dryer.

A hybrid smart solar dryer (HSSD) based on indirect forced convection and a controlled auxiliary heating system was developed, fabricated, and tested to be convenient for sunny and cloudy weather conditions. The achievements of the developed dryer focus on controlling the temperature of the dryer, increasing the drying rate, reducing energy consumption, and providing high-quality products. The HSSD was tested and evaluated for drying basil and sage herbs at 30, 40, and 50°C. The results showed that the fresh basil and sage leaves of 1 kg with a moisture content of 84.7% and 75.53% (wet basis) were dried within 58, 46, 32 and 38, 28, and 20 h at 30, 40, and 50°C, respectively. Correspondingly, the traditional drying methods achieved 96 h outdoors and 144 h indoors at room temperature. The average of the fabricated flat-plate solar collector efficiency (thermal efficiency, ηfpsc ) was ranged from 49.18% ± 9.52% to 66.02% ± 2.8%. The highest drying rates were achieved with the HSSD method. Moreover, the HSSD method led to a remarkable saving in energy with values ranging from 25.54% to 77.1% versus the traditional drying methods. While the best quality in terms of essential oil content and microbial load for the dried basil and sage herbs was achieved by the HSSD at 40°C. Finally, the HSSD is a promising energy-efficient method where it can save 70% of energy consumption, thus reducing the carbon footprint of drying processes, and providing higher quality products compared to the conventional methods.

Exploration of T cell immune responses by expression of a dominant-negative SHP1 and SHP2.

SHP1 and SHP2 are SH2 domain-containing proteins which have inhibitory phosphatase activity when recruited to phosphorylated ITIMs and ITSMs on inhibitory immune receptors. Consequently, SHP1 and SHP2 are key proteins in the transmission of inhibitory signals within T cells, constituting an important point of convergence for diverse inhibitory receptors. Therefore, SHP1 and SHP2 inhibition may represent a strategy for preventing immunosuppression of T cells mediated by cancers hence improving immunotherapies directed against these malignancies. Both SHP1 and SHP2 contain dual SH2 domains responsible for localization to the endodomain of inhibitory receptors and a protein tyrosine phosphatase domain which dephosphorylates and thus inhibits key mediators of T cell activation. We explored the interaction of the isolated SH2 domains of SHP1 and SHP2 to inhibitory motifs from PD1 and identified strong binding of both SH2 domains from SHP2 and more moderate binding in the case of SHP1. We next explored whether a truncated form of SHP1/2 comprising only of SH2 domains (dSHP1/2) could act in a dominant negative fashion by preventing docking of the wild type proteins. When co-expressed with CARs we found that dSHP2 but not dSHP1 could alleviate immunosuppression mediated by PD1. We next explored the capacity of dSHP2 to bind with other inhibitory receptors and observed several potential interactions. In vivo we observed that the expression of PDL1 on tumor cells impaired the ability of CAR T cells to mediate tumor rejection and this effect was partially reversed by the co-expression of dSHP2 albeit at the cost of reduced CAR T cell proliferation. Modulation of SHP1 and SHP2 activity in engineered T cells through the expression of these truncated variants may enhance T cell activity and hence efficacy in the context of cancer immunotherapy.

Novel Fas-TNFR chimeras that prevent Fas ligand-mediated kill and signal synergistically to enhance CAR T cell efficacy.

The hostile tumor microenvironment limits the efficacy of adoptive cell therapies. Activation of the Fas death receptor initiates apoptosis and disrupting these receptors could be key to increasing CAR T cell efficacy. We screened a library of Fas-TNFR proteins identifying several novel chimeras that not only prevented Fas ligand-mediated kill, but also enhanced CAR T cell efficacy by signaling synergistically with the CAR. Upon binding Fas ligand, Fas-CD40 activated the NF-κB pathway, inducing greatest proliferation and IFN-γ release out of all Fas-TNFRs tested. Fas-CD40 induced profound transcriptional modifications, particularly genes relating to the cell cycle, metabolism, and chemokine signaling. Co-expression of Fas-CD40 with either 4-1BB- or CD28-containing CARs increased in vitro efficacy by augmenting CAR T cell proliferation and cancer target cytotoxicity, and enhanced tumor killing and overall mouse survival in vivo. Functional activity of the Fas-TNFRs were dependent on the co-stimulatory domain within the CAR, highlighting crosstalk between signaling pathways. Furthermore, we show that a major source for Fas-TNFR activation derives from CAR T cells themselves via activation-induced Fas ligand upregulation, highlighting a universal role of Fas-TNFRs in augmenting CAR T cell responses. We have identified Fas-CD40 as the optimal chimera for overcoming Fas ligand-mediated kill and enhancing CAR T cell efficacy.

Dual targeting of CD19 and CD22 against B-ALL using a novel high-sensitivity aCD22 CAR.

CAR T cells recognizing CD19 effectively treat relapsed and refractory B-ALL and DLBCL. However, CD19 loss is a frequent cause of relapse. Simultaneously targeting a second antigen, CD22, may decrease antigen escape, but is challenging: its density is approximately 10-fold less than CD19, and its large structure may hamper immune synapse formation. The characteristics of the optimal CD22 CAR are underexplored. We generated 12 distinct CD22 antibodies and tested CARs derived from them to identify a CAR based on the novel 9A8 antibody, which was sensitive to low CD22 density and lacked tonic signaling. We found no correlation between affinity or membrane proximity of recognition epitope within Ig domains 3-6 of CD22 with CART function. The optimal strategy for CD19/CD22 CART co-targeting is undetermined. Co-administration of CD19 and CD22 CARs is costly; single CARs targeting CD19 and CD22 are challenging to construct. The co-expression of two CARs has previously been achieved using bicistronic vectors. Here, we generated a dual CART product by co-transduction with 9A8-41BBζ and CAT-41BBζ (obe-cel), the previously described CD19 CAR. CAT/9A8 CART eliminated single- and double-positive target cells in vitro and eliminated CD19- tumors in vivo. CAT/9A8 CART is being tested in a phase I clinical study (NCT02443831).

In ovo protective effects of chicoric and rosmarinic acids against Thiacloprid-induced cytotoxicity, oxidative stress, and growth retardation on newly hatched chicks.

Thiacloprid (TH) is a neonicotinoid insecticide employed in agriculture to protect fruits and vegetables against different insects. It showed different deleterious effects on the general health of non-target organisms including birds and animals, however, its developmental toxicity has yet to be fully elucidated. Chicoric (CA) and rosmarinic (RA) acids are polyphenolic compounds with a wide range of beneficial biological activities. In this study, the possible protective effects of CA and RA were investigated in chick embryos exposed in ovo to TH (1µg/egg) with or without CA (100 µg/egg) or RA (100 µg/egg) co-exposure. TH reduced the hatchling body weight, body weight/egg weight, and relative weight of bursa of Fabricius in the one-day-old hatchlings. Examination of the 7-day-old chicks revealed a decline in feed intake, daily weight gain, feed conversion ratio (FCR), and plasma levels of T3, T4, and growth hormone. Serum ALT, AST activities, and total cholesterol levels showed significant elevations. Hepatic MDA was increased with a reduction in SOD activity and GSH level and downregulation of the liver SOD and GST gene expression pattern. Serum IgG and IgM levels were reduced, and various histopathological alterations were noticed in the liver. Co-administration of CA or RA with TH mitigated the toxic effects on hatchlings. When both CA and RA are combined, they present a synergistic protective effect. CA and RA can be used as protective agents against TH toxicity as they improve growth performance and have hepatoprotective and immunostimulant effects in newly hatched chicks.

Effect of dietary modulation of fiber and fat level on liver and kidney functions, lipid profile, antioxidant and immune parameters in growing Egyptian geese.

Determining the optimal requirements from dietary fiber and fat for Egyptian geese is a matter of great concern regarding health, production and growth. Therefore, the current study estimated the effects of different dietary fiber and fat levels on functions of liver and kidney, lipid profile, immunity and antioxidant measurements of the growing Egyptian geese. 150 Egyptian goslings (4 weeks old), with almost the same body weights were randomly allocated into 6 groups (25 goslings/group). All groups were subdivided into five replicates, each replicate contains five geese. Liver and kidney functions, immunity and antioxidant parameters were not significantly affected the different studied levels of fiber and fat or by their interaction. Various levels of fiber significantly affected total cholesterol, triglycerides, LDL-cholesterol and VLDL-cholesterol in a concentration-dependent manner and the lowest the lipid profile values were obtained at 12%. In conclusion, the present findings show that use of dietary fiber up to 12% and 5% dietary fat showed no detrimental effects on the immune status and general health of geese and resulted in the preferable lipid profile. This experiment provides a base for further study about the optimal requirements from dietary fiber and fat for the growing Egyptian geese.

The role of different dietary Zn sources in modulating heat stress-related effects on some thermoregulatory parameters of New Zealand white rabbit bucks.

The present work was conducted to assess the effect of diets supplementation to heat-stressed buck rabbits with different zinc (Zn) sources on the thermoregulatory and hematobiochemical parameters, and antioxidant status. A total of 24 mature buck rabbits (32-36 weeks of age) were randomly distributed into four groups (6 each). Group 1, non-heat-stressed control (NHSC), was reared in the absence of heat stress (HS) conditions and received the basal diet only. The other three groups (groups 2, 3 and 4) were kept in HS conditions. Group 2, heat-stressed control (HSC), received the basal diet only. The diet supplemented with 75 mg Zn/kg diet either in the inorganic form (Zn sulfate) or in the organic form (Zn picolinate) for groups 3 and 4, respectively. Zn supplementation to rabbits' diets lowered the heat stress-related increase of serum urea, alanine transaminase and malondialdehyde (MDA) concentration. These supplementations also increased the concentration of testosterone under HS conditions. Zn picolinate was more effective than Zn sulfate in restoring serum concentrations of urea, testosterone, and MDA. In conclusion, Zn addition to rabbits' diets from different sources, especially Zn picolinate, exhibits an ameliorative effect against the harmful impact of HS on hematobiochemical parameters and antioxidant status.

Insights into enzyme activity and phosphorus conversion during kitchen waste composting utilizing phosphorus-solubilizing bacterial inoculation.

The main objective of this research was to investigate the effects of Phosphorus-Solubilizing Bacterial (PSB) inoculant on the bacterial structure and phosphorus transformation during kitchen waste composting. High throughput sequencing, topological roles, and multiple analysis methods were conducted to explain the links between phosphorus fractions, enzyme contents, and microbial community structure and function. The findings indicated that bacterial inoculant improved environmental parameters and increased the concentration of total phosphorus, Olsen phosphorus, citric acid phosphorus, OM decomposition, and bacterial diversity. Network analysis concluded that the inoculation treatment was more complex (nodes and edges) and contained more positive links than the control, implying the inoculation effect. The structural equation model also displayed that pH and enzyme activity directly enhanced the phosphorus conversion and bacterial structure. Overall, these results suggest that bacterial inoculation may considerably increase enzyme activity, thus improving biological phosphorus transformation and nutrient content in composting products.

Mitigating the detrimental effects of heat stress in poultry through thermal conditioning and nutritional manipulation.

The poultry industry faces several obstacles and challenges, including the changes in global temperature, increase in the per capita demand for meat and eggs, and the emergence and spread of various diseases. Among these, environmental challenges are one of the most severe hurdles impacting the growth and productivity of poultry. In particular, the increasing frequency and severity of heat waves over the past few years represent a major challenge, and this is expected to worsen in the coming decades. Chickens are highly susceptible to high ambient temperatures (thermal stress), which negatively affect their growth and productivity, leading to enormous economic losses. In the light of global warming, these losses are expected to increase in the near future. Specifically, the worsening of climate change and the rise in global temperatures have augmented the adverse effects of heat on poultry production worldwide. At present, the world population is approximately 7.9 billion, and it has been predicted to reach 9.3 billion by 2050 and approximately 11 billion by 2100, implying a great demand for protein supply; therefore, strategies to mitigate future poultry challenges must be urgently devised. To date, several mitigation measures have been adopted to minimize the negative effects of heat stress in poultry. Of these, thermal acclimation at the postnatal stage or throughout the embryonic stages has been explored as a promising approach; however, for large-scale application, this approach warrants further investigation to determine the suitable temperature and poultry age. Moreover, molecular mechanisms governing thermal conditioning are poorly understood. To this end, we sought to expand our knowledge of thermal conditioning in poultry, which may serve as a valuable reference to improve the thermotolerance of chickens via nutritional management and vitagene regulation. Vitagenes regulate the responses of poultry to diverse stresses. In recent years, nutritionists have paid close attention to bioactive compounds such as resveratrol, curcumin, and quercetin administered alone or in combination. These compounds activate vitagenes and other regulators of the antioxidant defense system, such as nuclear factor-erythroid 2-related factor 2. Overall, thermal conditioning may be an effective strategy to mitigate the negative effects of heat stress. In this context, the present review synthesizes information on the adverse impacts of thermal stress, elucidating the molecular mechanisms underlying thermal conditioning and its effects on the acquisition of tolerance to acute heat stress in later life. Finally, the role of some polyphenolic compounds, such as resveratrol, curcumin, and quercetin, in attenuating heat stress through the activation of the antioxidant defense system in poultry are discussed.

Consequences of varying dietary crude protein and metabolizable energy levels on growth performance, carcass characteristics and biochemical parameters of growing geese.

This research aimed to investigate the effect of varying dietary crude protein (CP) and metabolizable energy (ME) quantity on growth, carcass characteristics, biochemical blood parameters and molecular aspects of growing geese. Overall, a total of 180 Egyptian goslings were used in a 2 × 3 factorial design. Two levels of CP (18% and 16%) and three levels of ME (3000, 2900 and 2800 kcal/kg diet) were employed. Growth traits showed no significant differences as a result of different CP or ME levels. Birds fed the higher CP level consumed more feed than the lower. Carcass, dressing, heart, spleen, abdominal fat and thigh percentages within the group fed 18% CP were higher than the other group. Compared with the other ME-based diet, geese fed a low-ME based diet had the highest heart percentage. Feeding growing geese on a 2900 Kcal/kg ME-based diet increased the serum content of total protein and globulin. Geese fed the high-ME diet showed the highest levels of ALP and C4 as compared with other ME levels. Regarding Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), different protein and energy levels did not affect the molecular weight of blood proteins of Egyptian geese during the growing phase. It could be concluded the levels of 16% CP and 2800 kcal/kg ME are enough to maintain the growth performance and the other physiological indices as well as molecular aspects.

Development and evaluation of an innovative grain cart with a pneumatic conveyor.

In line with the requirements of the Egyptian government to find a solution for wheat transportation during the peak harvesting season, an innovative design for a grain cart with a capacity of 8 tons supplemented with a grain hopper, a lifting double-action pneumatic conveyor, and a built-in digital scale was tested and evaluated to facilitate the transport of wheat crops from farmers' fields to storage sites. The cart was manufactured in the workshop of a local industrial company. It was tested under varying operational conditions in different wheat production areas in terms of working performance, efficiency of the grain loading and unloading mechanism, precision of the grain weighing mechanism, and cost/ton. The cart will enable wheat farmers and traders to transport and deliver their crops easily to storage sites with minimal losses and maximum working efficiency. It will also increase farmers' profit because transportation cost by using this grain cart is less than that of the current conventional method. Moreover, the developed cart can secure wheat supply in government storage sites without any interruption during the peak wheat harvesting season.

Performance Analysis and Quality Evaluation of Wheat Storage in Horizontal Silo Bags.

Wheat still suffers from the problem of traditional storage methods, limited storage capacity, and a high percentage of losses in terms of quantity and quality. Hermetic silo bags are economical and alternative technique to the traditional storage methods. Ten horizontal plastic silos with the capacity of 200 tons/silo were tested and evaluated for eight months of wheat storage. The evaluations included grain bulk temperature, CO2 concentration, fungal and microbial count, insect count, grain moisture content, 1000-grain weight, falling number, and protein content. The results showed that the stored wheat quality was maintained without any significant difference during the storage period in terms of 1000-grain weight, grain moisture content, and falling number, while there were slight changes in protein content and kernel hardness with a decrease of 5.5% and 4.6% at the end of the storage period. There were no statistically significant differences at the sampling location along the length of the storage silos, which confirms the homogeneity of the internal conditions of the examined silo. The grain bulk temperature inside the silos was always lower than the surrounding ambient air temperature. The higher concentration of carbon dioxide inside the silos during the storage period led to a decrease in fungal and microbial count and the presence of dead insects at the end of the storage period.

Effect of dietary calcium and phosphorus levels on growth, carcass characteristics and liver and kidney functions of growing Egyptian geese.

The present study investigated the effect of different dietary levels of calcium (Ca) and non-phytate phosphorus (P) on the growth performance, carcass characteristics, and blood components of growing geese. A total of 120, 4-wk-old Egyptian goslings with similar body weights were randomly distributed to four groups in a 2 × 2 factorial arrangement, which included 2 levels of Ca (0.85% and 0.70%) and 2 levels of non-phytate P (0.45% and 0.35%). Each group was subdivided into 6 replicates of five birds. The experiment lasted 8 wk, from 4 to 12 wk of age. Results show that dietary Ca level had no significant effect on any of the studied growth performance traits over the full experimental period. Dietary P level also had no significant impact on these traits, with the exception of daily body weight gain and feed conversion ratio at 8 to 12 wk of age; these improved significantly with the low P diet. Geese received a diet containing 0.70% Ca + 0.45% P had the lowest body weight values at 12 wk of age and the lowest daily body weight gain, and feed intake at 8 to 12 weeks of age. While, the lowest value of feed conversion ratio was recorded in geese fed low level of Ca with low level of P (0.70% Ca + 0.35% P). There were no significant effects of the different dietary levels of Ca, P, or their interaction on all studied carcass parameters. Low dietary Ca level significantly increased the plasma levels of total protein, albumin, alanine transaminase (ALT), aspartate transaminase (AST), and creatinine and significantly decreased the plasma levels of Ca and P. Different dietary P levels had no significant effect on plasma levels of albumin, AST, ALT, ALP, and urea, whereas the 0.35% P-based diet significantly decreased the plasma contents of total protein, creatinine, Ca, and P. Plasma levels of albumin, creatinine, urea, Ca, and P were not affected by an interaction between Ca and P. Diets containing 0.70% Ca and 0.45% P lead to the highest plasma values for total protein, ALT, AST, and ALP compared with the other dietary Ca and P combinations. In conclusion, dietary Ca and P levels can be simultaneously reduced without negative impacts on growth performance, carcass characteristics, or blood biochemical components. We advise to avoid increasing the dietary Ca: P ratio, as it leads to negative effects on growth performance and blood biochemistry in growing geese. So, the findings of the current study recommended the low levels of Ca (0.70%) and non-phytate P (0.35%) for the performance of Egyptian geese during the fattening period.

Amer's classification of territories of facial nerve injury in early cases and strategies for the management of different territories.

Early cases of facial nerve injury are best treated by restoring the neural pathway to the same existing facial muscles. Knowledge of the exact territory of facial nerve injury is required to design a plane for the reconstruction of these injuries and to compare results. The current study aims to design a classification system for territories of facial nerve injury based on the location of nearest healthy fascicles to the site of injury both proximally and distally. Two hundred-one patients with early facial nerve injury were assessed for treatment. According to the results of the exploration, 13 territories of injury were identified. The management strategy was planned according to the territory of injury. The current classification system is a simple, easy and effective method for the classification of territories of facial nerve injury. The classification system accurately describes the nearest possible healthy proximal and distal fascicles and can be employed to easily report cases and implement a management plan. This classification scheme also allows us to more effectively compare results.