RESUMO
Earlier reports on the detection of Helicobacter DNA in the gallbladder tissue of patients with biliary diseases have shown discordant results. This study aimed to detect the presence of Helicobacter in gallstone, gallbladder tissue and bile specimens from subjects with H. pylori-positive gastritis with cholelithiasis. The presence of H. pylori in antrum biopsies was confirmed by rapid urease test and/or histopathological examination. DNA was extracted from gallbladder, bile and gallstone samples from 50 patients undergoing cholecystectomy. The presence of Helicobacter genus-specific DNA (16S rRNA genes) was determined by nested polymerase chain reaction assay. Helicobacter DNA was detected in the gallbladder tissue and bile of 28% and 18% respectively of the patients, but was not detected in any of the gallstones. These results do not rule out the possibility of Helicobacter infection as a contributing agent or cofactor in the development of biliary diseases.
Assuntos
Bile/microbiologia , Vesícula Biliar/microbiologia , Cálculos Biliares/epidemiologia , Infecções por Helicobacter/epidemiologia , Helicobacter pylori , Adulto , Idoso , Egito/epidemiologia , Feminino , Cálculos Biliares/microbiologia , Infecções por Helicobacter/patologia , Helicobacter pylori/isolamento & purificação , Humanos , Masculino , Pessoa de Meia-Idade , Reação em Cadeia da PolimeraseRESUMO
Earlier reports on the detection of Heticobocter DNA in the gallbladder tissue of patients with biliary diseases have shown discordant results. This study aimed to detect the presence of Helicobacter in gallstone, gallbladder tissue and bile specimens from subjects with H. pylori-positive gastritis with cholelithiasis. The presence of H. pylori in antrum biopsies was confirmed by rapid urease test and/or histopathological examination. DNA was extracted from gallbladder, bile and gallstone samples from 50 patients undergoing cholecystectomy. The presence of Helicobacter genus-specific DNA [16S rRNA genes] was determined by nested polymerase chain reaction assay. Helicobacter DNA was detected in the gallbladder tissue and bile of 28% and 18% respectively of the patients, but was not detected in any of the gallstones. These results do not rule out the possibility of Helicobacter infection as a contributing agent or cofactor in the development of biliary diseases
Assuntos
Vesícula Biliar , Cálculos Biliares , DNA , Reação em Cadeia da Polimerase , Helicobacter pyloriAssuntos
Fístula/cirurgia , Fístula Pancreática/cirurgia , Doenças Pleurais/cirurgia , Esfinterotomia Endoscópica/métodos , Stents , Colangiopancreatografia Retrógrada Endoscópica , Feminino , Fístula/diagnóstico por imagem , Seguimentos , Humanos , Pessoa de Meia-Idade , Fístula Pancreática/diagnóstico por imagem , Doenças Pleurais/diagnóstico por imagem , Esfinterotomia Endoscópica/instrumentação , Tomografia Computadorizada por Raios XRESUMO
The authors present their experience with right thoracic first approach in resection of upper thoracic esophageal carcinoma followed by gastro-esophageal anastomosis. This technique has got many advantages: any degree of tumoral extension can be managed by an adapted strategy for the situation; and the technique is particularly suitable for resection of upper thoracic esophageal lesions which usually present many hazard for their removal. Thirty three patients have been operated according to this technique. A satisfactory rate of complete resection was obtained. Mortality rate one month after the operation was of 6%, an although the extensive character of the lesions encountered in this survey of patients, long-life survival rate was satisfactory and seems that it could be ameliorated by the application of a preoperative radio-chemotherapy protocol.
