RESUMO
A green and scalable approach for the preparation of few-layered graphene utilizing the biowaste of potato peels has been developed. The potato peels have been dried and carbonized to obtain a new graphite structure that has been exfoliated in N-methylene phosphonic acid chitosan (MPC). The exfoliation process assisted the formation of graphene sheets with a high size diameter and quality of 50% based on the weight of graphite structure. The graphene sheets were green decorated with silver nanoparticles using microwave power to obtain new nanocomposites. The mass ratio between the graphite and silver nitrate was optimized and observed to change the morphology and size diameter of silver nanoparticles. The as-prepared MPC structure, graphene, and silver decorated graphene nanocomposites were characterized using 1HNMR, FTIR, XRD, UV/Vis spectrophotometer, SEM, and TEM besides tested as antimicrobial agents. The bacterial performance was also controlled by changing the number of AgNPs distributed on graphene sheets based on the mass ratios of graphite/AgNO3. The inhibition diameter of silver decorated graphene was considerably increased to 24.8, and 20.1 mm as in the case of MPC-GRP-Ag30 composite compared to the pure graphene (11.2, 13.5 mm) for E. coli and S. aureus, consecutively proposing that the blade edge of graphene sheets can destroy the bacteria membrane and release silver cations promptly that are directed for the interaction with the cytoplasmic parts of the bacteria cell. Such findings offer green and biocompatible antibacterial agents based on the graphene derived from the biowaste products.
Assuntos
Anti-Infecciosos/síntese química , Quitosana/análogos & derivados , Grafite/química , Nanopartículas Metálicas/química , Ácidos Fosforosos/química , Anti-Infecciosos/farmacologia , Química Verde/métodos , Prata/química , Staphylococcus aureus/efeitos dos fármacosRESUMO
This study evaluates the effect of DDB on normal and chemically-injured liver. When given to normal rats DDB had no significant effect on liver enzymes, but in chemically-injured rats there was a significant decrease in the elevated levels of liver enzymes. DDB produced a significant increase in reduced glutathione, glutathione peroxidase and glutathione reductase, and a significant decrease in malondialdehyde and glucose-6-phosphate dehydrogenase in both normal and chemically-injured liver. The histopathology examinations showed a slight improvement with DDB administration. DDB has a beneficial effect on liver enzymes and possesses significant antioxidant properties in normal and chemically-injured liver, and may therefore be clinically useful in treating chronic viral hepatitis B in humans.
Assuntos
Adjuvantes Imunológicos/uso terapêutico , Inibidores das Enzimas do Citocromo P-450 , Dioxóis/uso terapêutico , Modelos Animais de Doenças , Hepatopatias/tratamento farmacológico , Fígado/efeitos dos fármacos , Adjuvantes Imunológicos/farmacologia , Administração Oral , Animais , Antioxidantes/farmacologia , Antioxidantes/uso terapêutico , Tetracloreto de Carbono , Doença Hepática Induzida por Substâncias e Drogas , Dioxóis/farmacologia , Avaliação Pré-Clínica de Medicamentos , Medicamentos de Ervas Chinesas/farmacologia , Medicamentos de Ervas Chinesas/uso terapêutico , Glucosefosfato Desidrogenase/efeitos dos fármacos , Glucosefosfato Desidrogenase/metabolismo , Glutationa/efeitos dos fármacos , Glutationa/metabolismo , Glutationa Peroxidase/efeitos dos fármacos , Glutationa Peroxidase/metabolismo , Glutationa Redutase/efeitos dos fármacos , Glutationa Redutase/metabolismo , Fígado/metabolismo , Fígado/ultraestrutura , Hepatopatias/metabolismo , Hepatopatias/patologia , Testes de Função Hepática , Masculino , Malondialdeído/metabolismo , Ratos , Fatores de TempoRESUMO
This study evaluates the effect of DDB on normal and chemically-injured liver. When given to normal rats DDB had no significant effect on liver enzymes, but in chemically-injured rats there was a significant decrease in the elevated levels of liver enzymes. DDB produced a significant increase in reduced glutathione, glutathione peroxidase and glutathione reductase, and a significant decrease in malondialdehyde and glucose-6-phosphate dehydrogenase in both normal and chemically-injured liver. The histopathology examinations showed a slight improvement with DDB administration. DDB has a beneficial effect on liver enzymes and possesses significant antioxidant properties in normal and chemically-injured liver, and may therefore be clinically useful in treating chronic viral hepatitis B in humans
