Growth, hemato-biochemical, immune-antioxidant response, and gene expression in Nile tilapia (Oreochromis niloticus) received nano iron oxide-incorporated diets.

Nanotechnology has recently played a key role in tackling many aquacultures issues. Hence, the present study targets the evaluation of dietary inclusion of nano iron oxide (nFe2O3) on growth performance, hematology, immune-antioxidant responses, ionic regulation and expression of related genes in Nile tilapia (Oreochromis niloticus). Fish were fed supplementary nFe2O3 at rates of zero (control), 0.5, and 1 g/kg diet for 30 days. Obtained data demonstrated that nFe2O3 significantly (P < 0.05) augmented growth performance (final weight and length, body mass gain, specific growth rate, feed conversion ratio, and length gain rate). Hematological picture {RBCs, Hb, MCV, MCH and MCHC, and leukocytes interpretations (WBCs and monocytes)}; and biochemical indexes including (AST and ALT; total protein; and glucose, and cortisol) were significantly (P < 0.05) improved in nFe2O3 supplemented groups. Plasma ionic concentration was also altered with nFe2O3 supplementation, and 1g nFe2O3 revealed the most marked increase in plasma (Na+) potassium (K+) levels. Similarly, IgM, nitrous oxide (NO), and lysozyme activity, plus superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GPx) activities showed a remarkable improvement in 1g nFe2O3 group compared to the control. Expression of Insulin-Like Growth Factor-1 (IGF-1) and interleukin 1-ß (IL-1ß) genes were significantly up-regulated in nFe2O3 supplemented groups. Briefly, dietary nFe2O3 inclusion had enhanced properties on growth; hemato-biochemical; immune, antioxidative profiles; and related genes expression of O. niloticus, with a recommended concentration of 1g nFe2O3.

Foliar Application of Nano, Chelated, and Conventional Iron Forms Enhanced Growth, Nutritional Status, Fruiting Aspects, and Fruit Quality of Washington Navel Orange Trees (Citrus sinensis L. Osbeck).

Iron (Fe) is required for most metabolic processes, including DNA synthesis, respiration, photosynthesis, and chlorophyll biosynthesis; however, Fe deficiency is common in arid regions, necessitating additional research to determine the most efficient form of absorbance. Nano-fertilizers have characteristics that are not found in their traditional equivalents. This research was implemented on Washington navel orange trees (Citrus sinensis L. Osbeck) to investigate the effect of three iron forms-nano (Fe-NPs), sulfate (FeSO4), and chelated (Fe-chelated)-as a foliar spray on the growth, fruiting aspects, and nutritional status of these trees compared to control. The highest values of the tested parameters were reported when the highest Fe-NPs level and the highest Fe-chelated (EDTA) rate were used. Results obtained here showed that the spraying of the Washington navel orange trees grown under similar environmental conditions and horticulture practices adopted in the current experiment with Fe-NPs (nanoform) and/or Fe-chelated (EDTA) at 0.1% is a beneficial application for enhancing vegetative growth, flower set, tree nutritional status, and fruit production and quality. Application of Fe-NPs and Fe-chelated (EDTA, 0.1%) increased yield by 32.0% and 25% and total soluble solids (TSS) by 18.5% and 17.0%, respectively, compared with control. Spraying Washington navel orange trees with nano and chelated iron could be considered a significant way to improve vegetative growth, fruit production, quality, and nutritional status while also being environmentally preferred in the arid regions.

Impacts of Effective Microorganisms, Compost Tea, Fulvic Acid, Yeast Extract, and Foliar Spray with Seaweed Extract on Sweet Pepper Plants under Greenhouse Conditions.

Sweet pepperincludes several vitamins and is regarded as a great source of bioactive nutrients, such as carotenoids and phenolic compounds, for human growth and activities. This work aimed to investigate the effects of the soil addition of growth stimulants, namely, effective microorganisms (EM), compost tea, fulvic acid, and yeast extract, and foliar applications of seaweed extract, on the vegetative growth, enzyme activity, phytohormones content, chemical constituents of plant foliage, fruit yield, and fruit quality of sweet pepper plants (Capsicum annuum L. cv. Zidenka) growing under greenhouse conditions. The results showed that the tallest plant, largest leaf area/plant, and heaviest plant fresh and dry weights were recorded after combining a soil addition of yeast extract and foliar spray with seaweed extracts at 3 g/L in two growing seasons. The highest number of fruit/plant, fruit yield/m2, fruit values of vitamin C (VC), total sugars, total soluble solids (TSS), and carotenoids, along with the highest leaf of cytokines, P, K, Fe, and total carbohydrates values, were obtained using a soil addition of fulvic acid and spray with seaweed extract at 3 g/L in the two seasons of study. These treatments also provided the lowest abscisic acid, peroxidase, and super oxidase dismutase values in the same conditions. Sweet pepper plants supplemented with compost tea and seaweed extract foliar spray at 3 g/L were the most promising for inducing the highest values of fruit fresh and dry weights, fruit length and diameter, and the leavesrichest in N, Zn, and Mn; inversely, it induced the lowest catalase levels in both seasons. The applications of EM, yeast extract, and seaweed extract could be applied for high growth, mineral levels, enzymatic activity, fruit yield, and nutritional value of sweet pepper fruit and minimizing environmental pollution.