Your browser doesn't support javascript.
loading
Mostrar: 20 | 50 | 100
Resultados 1 - 20 de 23
Filtrar
1.
Water Sci Technol ; 85(11): 3240-3258, 2022 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-35704408

RESUMO

The study evaluated the use of Chlorella vulgaris for bioremediating hydroponic wastewater and producing biomass under different cultivation modes and to explore the economic implications of microalgal biofuels. Total nitrogen (TN) removal efficiency was 98.5% in mixotrophic conditions and 96% in heterotrophic conditions, and total phosphorus (TP) was completely removed (>99%) in both cultivation conditions. TN removal was higher for that which was cultivated under the mixotrophic mode of cultivation. The maximum biomass production (1.26 g/L) and biomass productivity (0.1108 g/L/day) were also reported for mixotrophic conditions. Lipid content was slightly higher for that which was cultivated under heterotrophic conditions: 33 wt% on an ash-free dry weight (AFDW) basis. The highest lipid production was obtained under mixotrophic growth (0.341 g/L). Higher net profit was obtained for both mixotrophic and heterotrophic cultivations: 30.6 million $/year for a plant capacity of 3.29 × 104 tone/year and 30.12 million $/year for a plant capacity of 3.17 × 104 tone/year respectively. Sensitivity analysis showed that biodiesel and nutritious supplements from soluble protein have the greatest impact on the process economics regarding mixotrophic cultivation, while biodiesel and feeds from insoluble protein have the largest effect on the process economics regarding heterotrophic and autotrophic cultivations.


Assuntos
Chlorella vulgaris , Microalgas , Biocombustíveis , Biomassa , Chlorella vulgaris/metabolismo , Hidroponia , Lipídeos , Microalgas/metabolismo , Nitrogênio/metabolismo , Nutrientes , Águas Residuárias
2.
J Appl Microbiol ; 126(2): 443-451, 2019 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-30142693

RESUMO

AIMS: This study aimed to highlight the importance of compost from aromatic plants as a stunning source for several bio active compounds generated from their inhabited thermophilic bacteria. Some of the isolated compounds could have a potential role in the treatment of microbial infections. METHODS AND RESULTS: A total of forty different thermophilic bacteria were isolated from compost samples during their thermophilic stage. These isolates were tested for their antimicrobial capabilities against different Gram-positive and -negative bacteria using agar diffusion and double layer agar methods. The potential isolates were further identified based on morphological, biochemical and 16S rRNA gene sequencing methods. They were subjected to submerged state fermentation and the total crude metabolites were recovered using ethyl acetate (EtOAc) extraction. All bioactive metabolites were identified using liquid chromatography coupled with high-resolution mass spectrometry (LC-HRMS). It was observed that 2 out of 40 isolates were remarkably active against Gram-positive bacteria. These isolates were genetically identified as Bacillus species and their different active metabolites were characterized in the EtOAc extracts using LC-HRMS. CONCLUSION: Liquid chromatography coupled with high-resolution mass spectrometry analysis of EtOAc extracts revealed the presence of active metabolites that are responsible for antimicrobial activities. SIGNIFICANCE AND IMPACT OF THE STUDY: To the best of our knowledge, this is the first time to identify bioactive antimicrobial metabolites from retrieved compost micro-organisms in Egypt. So, compost could be a beneficial area for research as a reliable and continuous natural source for different uncountable communities of bacteria.


Assuntos
Antibacterianos/metabolismo , Bactérias/metabolismo , Compostagem , Antibacterianos/farmacologia , Bacillus/isolamento & purificação , Bacillus/metabolismo , Bactérias/citologia , Bactérias/isolamento & purificação , Fermentação , Bactérias Gram-Positivas/efeitos dos fármacos , Interações Microbianas , Plantas
3.
Water Sci Technol ; 75(7-8): 1667-1674, 2017 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-28402308

RESUMO

Experiments in the field environment have been conducted to study the growth of Panicum repens L., an aquatic plant, in the sewage sludge matrix. The experiments were also carried out to investigate the ability of this plant to dewater sewage sludge to increase the capacity of conventional drying beds. In addition, the ability of Panicum repens L. to reduce the sludge contents of certain elements (copper (Cu), Iron (Fe), Sodium (Na), lead (Pb), and Zinc (Zn)) was also investigated. All experiments were carried out in batch reactors. Different plant coverage densities were tested (0.00 to 27.3 kg/m2). The liquid sewage sludge was collected from a wastewater treatment plant in Helwan city, Cairo Governorate, Egypt. The collected sludge represents a mixture of the primary sludge and waste activated sludge before discharging into drying beds.


Assuntos
Panicum/metabolismo , Esgotos/química , Biodegradação Ambiental , Cobre/análise , Egito , Água/análise , Zinco/análise
4.
Res Vet Sci ; 108: 25-32, 2016 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-27663366

RESUMO

Tylvalosin (TVS) is a third-generation macrolide drug used for prophylaxis and treatment of mycoplasma, however; it is supposed to possess an immunosuppressive effect. In the current study, the immunosuppressive effect of TVS and florfenicol (FFC) and the potential immunomodulatory role of Vit E were investigated. The experiment included one day old chick groups treated with either TVS, FFC, Vit E, TVS/Vit E, FFC/Vit E and control non-treated group. Chicks were vaccinated with inactivated H9N2 avian influenza (AI) vaccine and humoral antibody titers to viral antigen as well as innate immunity (serum lysozyme activity and nitric oxide levels) were evaluated. Total and differential leucocytic counts, serum liver enzymes level, blood leucocytic DNA damage and cellular area percentages within the lymphoid organs were also screened. Treatment with TVS and FFC significantly decreased immune response of chickens while treatment with Vit E improved the humoral immune response at 4 and 5weeks post-vaccination. Vit E also significantly increased the cellular immune response. The combination of Vit E with either TVS or FFC modulated their immunosuppressive effect and resulted in mild immunostimulatory effects. TVS alone induced a genotoxic effect on chickens' blood leucocytes and the genotoxicity was inhibited by combination of TVS with Vit E. Histopathology revealed that chickens treated with either TVS or FFC exhibited toxic effect on the lymphatic tissues.


Assuntos
Adjuvantes Imunológicos/administração & dosagem , Galinhas/imunologia , Tolerância Imunológica/efeitos dos fármacos , Imunidade Humoral/efeitos dos fármacos , Imunidade Inata/efeitos dos fármacos , Vitamina E/imunologia , Vitaminas/imunologia , Animais , Antibacterianos/efeitos adversos , Antígenos Virais/farmacologia , Vírus da Influenza A Subtipo H9N2/imunologia , Vacinas contra Influenza/imunologia , Tianfenicol/efeitos adversos , Tianfenicol/análogos & derivados , Tilosina/efeitos adversos , Tilosina/análogos & derivados , Vacinas de Produtos Inativados/imunologia , Vitamina E/administração & dosagem , Vitaminas/administração & dosagem
5.
Br Poult Sci ; 55(6): 830-6, 2014.
Artigo em Inglês | MEDLINE | ID: mdl-25229385

RESUMO

1. The pharmacokinetics of difloxacin were investigated in healthy and E. coli-infected broiler chickens following intravenous and oral administration of a single dose of 10 mg/kg bodyweight. 2. After intravenous injection of difloxacin, the serum concentration-time curves were best described by a two-compartment open model. The distribution and elimination half-lives (t0.5α) and (t0.5el), respectively, were 0.10 ± 0.016 h and 3.7 ± 0.08 h in healthy chickens compared with 0.05 ± 0.005 h and 6.42 ± 0.71 h in E. coli-infected birds. The volumes of distribution Vdss were 3.14 ± 0.11 and 9.25 ± 0.43 l/kg, with total body clearance (Cltot) of 0.65 ± 0.018 and 1.14 ± 0.1 ml/kg/h, respectively. 3. Following oral administration, difloxacin was absorbed with t0.5(ab) of 0.57 ± 0.06 and 0.77 ± 0.04 h and was eliminated with t0.5(el) of 4.7 ± 0.34 and 3.42 ± 0.19, respectively, in normal and infected chickens. The peak serum concentrations were 1.34 ± 0.09 and 1.05 ± 0.06 µg/ml and attained a Tmax of 2.27 ± 0.07 and 2.43 ± 0.06 h, respectively. The systemic bioavailability of difloxacin following oral administration was 86.2% in healthy chickens and 90.6% in E. coli-infected birds. The minimum inhibitory concentration (MIC) and the minimum bactericidal concentration (MBC) of difloxacin against the field strain of E. coli O78 in vitro were 0.02 µg and 0.04 µg/ml, respectively. 4. These results show that administration of a therapeutic dose of difloxacin is effective in the treatment of E. coli infection in chickens. The serum concentration of the drug was much higher than the MIC of the E. coli O78 strain in both healthy and infected chickens.


Assuntos
Antibacterianos/farmacocinética , Galinhas/metabolismo , Ciprofloxacina/análogos & derivados , Infecções por Escherichia coli/veterinária , Doenças das Aves Domésticas/metabolismo , Administração Oral , Animais , Antibacterianos/administração & dosagem , Antibacterianos/sangue , Antibacterianos/uso terapêutico , Galinhas/microbiologia , Ciprofloxacina/administração & dosagem , Ciprofloxacina/sangue , Ciprofloxacina/farmacocinética , Ciprofloxacina/uso terapêutico , Infecções por Escherichia coli/tratamento farmacológico , Infecções por Escherichia coli/metabolismo , Feminino , Injeções Intravenosas/veterinária , Masculino , Testes de Sensibilidade Microbiana/veterinária , Doenças das Aves Domésticas/tratamento farmacológico , Doenças das Aves Domésticas/microbiologia
6.
Epidemiol Infect ; 140(2): 299-310, 2012 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-21470441

RESUMO

Diversity within Shigella dysenteriae (n=40) and Shigella boydii (n=30) isolates from children living in Egypt aged <5 years was investigated. Shigella-associated diarrhoea occurred mainly in summer months and in children aged <3 years, it commonly presented with vomiting and fever. Serotypes 7 (30%), 2 (28%), and 3 (23%) accounted for most of S. dysenteriae isolates; 50% of S. boydii isolates were serotype 2. S. dysenteriae and S. boydii isolates were often resistant to ampicillin, chloramphenicol and tetracycline (42%, 17%, respectively), although resistance varied among serotypes. Pulsed-field gel electrophoresis separated the isolates into distinct clusters correlating with species and serotype. Genetic differences in trimethoprim/sulfamethoxazole and ß-lactam-encoding resistance genes were also evident. S. dysenteriae and S. boydii are genetically diverse pathogens in Egypt; the high level of multidrug resistance associated with both pathogens and resistance to the most available inexpensive antibiotics underlines the importance of continuing surveillance.


Assuntos
Farmacorresistência Bacteriana , Disenteria Bacilar/epidemiologia , Shigella boydii/efeitos dos fármacos , Shigella boydii/isolamento & purificação , Shigella dysenteriae/efeitos dos fármacos , Shigella dysenteriae/genética , Antibacterianos/farmacologia , Pré-Escolar , Disenteria Bacilar/microbiologia , Egito/epidemiologia , Eletroforese em Gel de Campo Pulsado , Fezes/microbiologia , Feminino , Humanos , Lactente , Recém-Nascido , Masculino , Filogenia , Reação em Cadeia da Polimerase , Shigella boydii/classificação , Shigella boydii/genética , Shigella dysenteriae/classificação , Shigella dysenteriae/isolamento & purificação , Sulfametoxazol/farmacologia , beta-Lactamas/farmacologia
7.
J Int Med Res ; 39(5): 1573-88, 2011.
Artigo em Inglês | MEDLINE | ID: mdl-22117959

RESUMO

Psoriasis is a common, chronic, systemic, inflammatory disease of the skin that is often associated with inflammatory musculoskeletal disease. Psoriasis impacts on affected individuals and on society at many levels, being associated with considerable economic burden and impaired quality of life. This article aims to provide dermatologists and their allied healthcare professionals, particularly those practicing in Africa and the Middle East, with a review of the current understanding of psoriasis, its treatment and impact, as a backdrop for further discussion of the management of psoriasis in these regions. Insight into the real-life, day-to-day challenges and unmet needs currently facing dermatologists in Africa and the Middle East is provided by the authors, most of whom are experienced dermatologists practicing in this region.


Assuntos
Psoríase/tratamento farmacológico , África , Artrite Psoriásica/diagnóstico , Artrite Psoriásica/prevenção & controle , Doenças Cardiovasculares/etiologia , Dermatologia , Gerenciamento Clínico , Humanos , Oriente Médio , Guias de Prática Clínica como Assunto , Psoríase/complicações , Psoríase/diagnóstico , Fatores de Risco
8.
Water Environ Res ; 78(9): 951-64, 2006 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-17120455

RESUMO

Experiments were carried out to investigate the ability of water hyacinth (Eichhornia crassipes) to remove five heavy metals (cadmium, chromium, copper, nickel, and lead) commonly found in leachate. All experiments were conducted in batch reactors in a greenhouse. It was found that living biomass of water hyacinth was a good accumulator for copper, chromium, and cadmium. The plants accumulated copper, chromium, and cadmium up to 0.96, 0.83, and 0.50%, respectively, of their dry root mass. However, lead and nickel were poorly accumulated in water hyacinth. Also, nonliving biomass of water hyacinth dry roots showed ability to accumulate all metals, except Cr(VI), which was added in anionic form. The highest total metal sorption by nonliving dry water hyacinth roots was found to take place at pH 6.4. The current research demonstrates the potential of using water hyacinth for the treatment of landfill leachate containing heavy metals.


Assuntos
Eichhornia/metabolismo , Metais/isolamento & purificação , Poluentes Químicos da Água/química , Purificação da Água/métodos , Biodegradação Ambiental , Umidade , Concentração de Íons de Hidrogênio , Metais/química , Raízes de Plantas , Temperatura , Fatores de Tempo , Poluição Química da Água/prevenção & controle
9.
Clin Microbiol Infect ; 10(9): 804-10, 2004 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-15355411

RESUMO

Antimicrobial susceptibility testing was performed on 3,627 isolates of Escherichia coli and 180 isolates of Shigella spp. collected in rural locations from 875 Egyptian children with diarrhoea between 1995 and 2000. The cumulative rates of resistance for E. coli and Shigella spp. were high (respectively, 68.2% and 54.8% for ampicillin, 24.2% and 23.5% for ampicillin-sulbactam, 57.2% and 42.5% for trimethoprim-sulphamethoxazole, and 50.9% and 75.4% for tetracycline). Non-enterotoxigenic E. coli (NETEC) isolates had a consistently higher level of antimicrobial resistance than did enterotoxigenic E. coli (ETEC) isolates. Trend testing showed significant decreases in resistance to ampicillin, ampicillin-sulbactam and tetracycline among all E. coli isolates. Increasing rates of resistance were observed for trimethoprim-sulphamethoxazole in ETEC isolates and Shigella spp., but not in NETEC isolates. Low levels of resistance were observed for all other antimicrobial agents tested. Overall, high levels, but decreasing trends, of resistance to commonly used antimicrobial agents were detected among isolates of E. coli and Shigella spp. from children in rural Egypt.


Assuntos
Antibacterianos/farmacologia , Diarreia/microbiologia , Escherichia coli/efeitos dos fármacos , População Rural , Shigella/efeitos dos fármacos , Criança , Pré-Escolar , Farmacorresistência Bacteriana , Disenteria Bacilar/microbiologia , Egito , Enterotoxinas/metabolismo , Escherichia coli/imunologia , Escherichia coli/isolamento & purificação , Infecções por Escherichia coli/microbiologia , Infecções por Escherichia coli/prevenção & controle , Vacinas contra Escherichia coli/administração & dosagem , Feminino , Humanos , Lactente , Masculino , Testes de Sensibilidade Microbiana , Shigella/isolamento & purificação
10.
Environ Technol ; 25(7): 833-40, 2004 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-15346865

RESUMO

Batch experiments were conducted to investigate the effect of municipal landfill leachate pH on the growth of water hyacinth (Eichhornia crassipes). These experiments were carried out in a green house environment on leachate samples collected from Essex-Windsor Regional Landfill, Windsor, Ontario, Canada. It was found that water hyacinth plants survived in a pH range of 4.0 to 8.0. Both alkaline pH (above 8.0) and highly acidic pH (below 4.0) had inhibitory effect on the growth of plants. The pH range, for optimum growth of the water hyacinth plants was found to be 5.8 to 6.0. At optimum growth, water hyacinth had an average mean relative growth rate of 0.043 d-1. It was found that nitrogen compounds underwent different transformations depending on the pH of leachate. Plant uptake, nitrification and volatilization were among these transformations.


Assuntos
Eichhornia/crescimento & desenvolvimento , Nitrogênio/isolamento & purificação , Eliminação de Resíduos , Poluentes do Solo/análise , Poluentes da Água/análise , Eichhornia/química , Concentração de Íons de Hidrogênio , Solubilidade
11.
Egypt J Immunol ; 11(2): 183-9, 2004.
Artigo em Inglês | MEDLINE | ID: mdl-16734132

RESUMO

We measured interferon-gamma (IFN-gamma) in supernatant of peripheral blood mononuclear cell (PBMC) cultures stimulated with mycobacterial antigens. The study included ten children with newly diagnosed active pulmonary tuberculosis (TB), ten with inactive TB and 8 healthy controls. IFN-gamma was quantified by ELISA. Antigen stimulated PBMCs produced IFN-gamma at significantly higher levels than the background levels of nonstimulated cultures. Following specific antigen stimulation in vitro with TB bacilli, the PBMCs from patients with active and inactive TB produced significantly higher levels of IFN-gamma (433.15+/-119.80 and 420.64+/-131.52 respectively) than controls (241.2+/-119.74), (P< 0.001). However, no significant difference was found between active and inactive TB patients. In conclusion, estimation of specific IFN-gamma production by PBMC may be a useful immunological tool for diagnosis of childhood tuberculosis.


Assuntos
Interferon gama/análise , Leucócitos Mononucleares/imunologia , Mycobacterium tuberculosis/imunologia , Tuberculose Pulmonar/diagnóstico , Tuberculose Pulmonar/imunologia , Adolescente , Criança , Pré-Escolar , Feminino , Humanos , Testes Imunológicos/métodos , Interferon gama/metabolismo , Leucócitos Mononucleares/metabolismo , Masculino , Valor Preditivo dos Testes , Tuberculose Pulmonar/sangue
12.
Boll Chim Farm ; 141(1): 8-14, 2002.
Artigo em Inglês | MEDLINE | ID: mdl-12064063

RESUMO

2-Aminobenzamides (3a-g) were prepared starting with isatoic anhydride. These compounds reacted with chloroacetic acid to give (4a-g), which were cyclized by dicyclohexylcorbodiimide to afford (5a-g). The preliminary pharmacological screening revealed that some of the new 2H-1,4-benzodiazepin-3,5(1H,4H)-dione derivatives (5) exhibited CNS-depressant and anticonvulsant activities.


Assuntos
Anticonvulsivantes/síntese química , Anticonvulsivantes/farmacologia , Benzodiazepinas/síntese química , Benzodiazepinas/farmacologia , Depressores do Sistema Nervoso Central/síntese química , Depressores do Sistema Nervoso Central/farmacologia , Animais , Convulsivantes/farmacologia , Feminino , Indicadores e Reagentes , Espectroscopia de Ressonância Magnética , Masculino , Camundongos , Pentilenotetrazol/antagonistas & inibidores , Pentilenotetrazol/farmacologia , Sono/efeitos dos fármacos , Espectrofotometria Infravermelho
13.
Arch Pharm Res ; 24(1): 21-6, 2001 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-11235807

RESUMO

This manuscript reports the synthesis of two series of Mannich Bases 3-12 and 21-40 obtained respectively by the reaction of either 2-ethoxycarbonylindoles 1-2 or 5H-pyridazino [4,5b]inoles 17-20 as a substrate with formalin and the appropriate 2 degrees amines under the suitable Mannich conditions. Fourteen of the synthesized Mannich basese were screened as antihypetensive agents in normotensive anesthetized rats. The effect of compound 4 in normotensive anesthetized dogs was also studied.


Assuntos
Anti-Hipertensivos/síntese química , Indóis/síntese química , Bases de Mannich/síntese química , Animais , Anti-Hipertensivos/farmacologia , Pressão Sanguínea/efeitos dos fármacos , Cães , Indóis/farmacologia , Bases de Mannich/farmacologia , Ratos
14.
J Health Popul Nutr ; 18(1): 33-8, 2000 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-11014768

RESUMO

While Campylobacter, Salmonella, and Shigella remain major contributors to acute enteric infections, few studies on these pathogens have been conducted in Egypt. From January 1986 to December 1993, 869 Salmonella, Shigella and Campylobacter strains were isolated from stool specimens from 6,278 patients, presenting to the Abbassia Fever Hospital, Cairo, Egypt, with acute enteric infections. Salmonella predominated, totalling 465 isolates, followed by Shigella with 258 isolates, and Campylobacter with 146 isolates. Of the Shigella isolates, 124 were Shigella flexneri, 49 were S. sonnei, 47 were S. dysenteriae (mainly serotype 1, 2, and 3), and 38 were S. boydii. Campylobacter spp. comprised 92 Campylobacter jejuni and 54 C. coli isolates. Isolation of Salmonella was highest during the months of February-March, June-July, and October-November, while that of Shigella was maximal from July to October. Isolation of Campylobacter increased during May-June and again during August-October. Although Salmonella was sensitive to amikacin, aztreonam, ceftriaxone, and nalidixic acid, it was, however, resistant to erythromycin, streptomycin, ampicillin, chloramphenicol, and tetracycline. Shigella (> 80%) was sensitive to amikacin, ceftriaxone, cephalothin, sulphamethoxazole-trimethoprim (except S. sonnei), aztreonam, and nalidixic acid. Resistance (> 50%) was noted only for ampicillin, chloramphenicol, and tetracycline. C. jejuni and C. coli were resistant to cephalothin, aztreonam, and streptomycin. Some of the above antibiotics were employed to characterize the Egyptian isolates, but did not have any clinical utility in the treatment of diarrhoea. Significant differences (p < 0.05) were observed in the resistance profiles of Shigella and Salmonella between late 1980s and early 1990s. The results suggest the use of fluoroquinolones or a third-generation cephalosporin as an empirical treatment of enteric diseases. However, alternative control strategies, including the aggressive development of broadly protective vaccines, may be more effective approaches to curbing morbidity and mortality due to acute enteric infections.


Assuntos
Antibacterianos/farmacologia , Infecções Bacterianas/microbiologia , Campylobacter/isolamento & purificação , Diarreia/microbiologia , Salmonella/isolamento & purificação , Shigella/isolamento & purificação , Adolescente , Adulto , Antibacterianos/uso terapêutico , Infecções Bacterianas/tratamento farmacológico , Infecções Bacterianas/epidemiologia , Campylobacter/efeitos dos fármacos , Campylobacter/crescimento & desenvolvimento , Criança , Pré-Escolar , Diarreia/tratamento farmacológico , Diarreia/epidemiologia , Resistência Microbiana a Medicamentos , Egito/epidemiologia , Fezes/microbiologia , Feminino , Humanos , Masculino , Testes de Sensibilidade Microbiana , Salmonella/efeitos dos fármacos , Salmonella/crescimento & desenvolvimento , Estações do Ano , Shigella/efeitos dos fármacos , Shigella/crescimento & desenvolvimento
15.
Arch Pharm Res ; 23(4): 310-4, 2000 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-10976575

RESUMO

The synthesis and spectral analysis of some new 1,3-dihydro-3-hydroxy-3-[2-hydroxyimino-2-(substituted phenyl)ethyl]-2H-indol-2-ones (21-32) and spiro[3H-indol-3,5'-(4'H)-isoxazol]-2(1H)-ones (33-44) are described. Sixteen of the synthesized compounds were screened in vitro for their growth inhibitory activity against thirteen species of microorganisms, viz, S. aureus, S. epidermidis, S. faecalis, B. subtilis, B. cereus, E. aerogens, E. coli, P. aeruginosa, P. vulgaris, A. baumonia, A. faecalis, C. albicans and S. cervicae. Most of the compounds exhibited significant antimicrobial activity especially the oximes 28 and 29.


Assuntos
Antibacterianos/síntese química , Indóis/síntese química , Isoxazóis/síntese química , Oximas/síntese química , Antibacterianos/farmacologia , Bactérias/efeitos dos fármacos , Indóis/farmacologia , Isoxazóis/farmacologia , Testes de Sensibilidade Microbiana , Oximas/farmacologia , Oxindóis , Relação Estrutura-Atividade
16.
J Clin Microbiol ; 37(3): 873-4, 1999 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-9986881

RESUMO

In a population-based study of diarrhea in rural, northern Egypt, 60 Shigella flexneri strains were identified, of which 10 could not be definitively serotyped. Serological analysis with commercial reagents suggested that they were serotype 1, but the strains failed to react with subserotype 1a- or 1b-specific antibodies. All 10 strains reacted with MASF 1c, a monoclonal antibody specific for a provisional S. flexneri subserotype, 1c, first identified in Bangladesh and not previously detected outside of that region. Our results show that S. flexneri subserotype 1c is not unique to Bangladesh and that the inability to detect it may reflect both the limited use of suitable screening methods and the rarity of this subserotype.


Assuntos
Diarreia/microbiologia , Disenteria Bacilar/diagnóstico , Shigella flexneri/classificação , Anticorpos Antibacterianos/análise , Anticorpos Monoclonais , Antígenos de Bactérias/imunologia , Disenteria Bacilar/imunologia , Disenteria Bacilar/microbiologia , Egito , Fezes/microbiologia , Humanos , Kit de Reagentes para Diagnóstico , População Rural , Sorotipagem , Shigella flexneri/isolamento & purificação
17.
Pediatr Infect Dis J ; 17(9): 816-9, 1998 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-9779768

RESUMO

OBJECTIVES: To evaluate the efficacy and safety of azithromycin compared with rifampin for eradication of nasopharyngeal carriage of Neisseria meningitidis METHODS: Pharyngeal swabs were obtained from 500 students attending nursing school in Cairo, Egypt, to determine the colonization rate with N. meningitidis. Colonized individuals were randomized to receive azithromycin (500 mg once) or rifampin (600 mg twice daily for four doses). Subjects were then recultured 1 and 2 weeks posttreatment to determine the effectiveness of the antibiotic therapy for eradication of meningococcal nasopharyngeal colonization. RESULTS: Individuals treated with azithromycin had a 93% eradication rate at 1 and 2 weeks posttreatment comparable with 95 and 91%, respectively, for rifampin. No significant side effects were reported by any subjects treated with either antibiotic. CONCLUSION: Azithromycin is effective in the eradication of N. meningitidis from the nasopharynx of asymptomatic colonized individuals and deserves further evaluation for use as prophylaxis against N. meningitidis.


Assuntos
Antibacterianos/uso terapêutico , Antibióticos Antituberculose/uso terapêutico , Azitromicina/uso terapêutico , Portador Sadio/tratamento farmacológico , Infecções Meningocócicas/tratamento farmacológico , Rifampina/uso terapêutico , Adolescente , Adulto , Humanos , Nasofaringe/microbiologia , Neisseria meningitidis/efeitos dos fármacos , Neisseria meningitidis/isolamento & purificação
20.
J Clin Microbiol ; 34(5): 1216-9, 1996 May.
Artigo em Inglês | MEDLINE | ID: mdl-8727905

RESUMO

The conservation of flagellin genes from thermophilic Campylobacter spp. strains isolated in Egypt was evaluated by a restriction fragment length polymorphism (RFLP) assay. The flaA and flaB genes were amplified from 59 independent clinical isolates and digested with EcoRI and PstI, and the resulting patterns were compared with each other and with previously described RFLP groups. The results indicate that the isolates fell into 14 groups for flaA and 11 groups for flaB, 9 of which have been described, and that considerable genetic variability exists among isolates belonging to the same LIO serogroup. In most cases, the flaB gene displayed the same RFLP pattern as that of the flaA gene of the same strain, although some variability was observed. The data suggest that more variability of flagellin genes exists within the LIO serogroups common to Campylobacter field isolates from Egypt than has previously been reported for North American isolates.


Assuntos
Campylobacter coli/genética , Campylobacter jejuni/genética , Flagelina/genética , Genes Bacterianos , Polimorfismo de Fragmento de Restrição , Sequência de Bases , Infecções por Campylobacter/microbiologia , Campylobacter coli/classificação , Campylobacter coli/isolamento & purificação , Campylobacter jejuni/classificação , Campylobacter jejuni/isolamento & purificação , Estudos de Casos e Controles , Criança , Sequência Conservada , Diarreia/microbiologia , Egito , Humanos , Sorotipagem , Especificidade da Espécie
SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA
...