Temporal phytoremediation potential for heavy metals and bacterial abundance in drainage water.

Drainage water in developing countries has a high abundance of pathogenic bacteria and high levels of toxic and mutagenic pollutants. Remediation of drainage water is important in water-poor counties, especially with the growing need to secure sustainability of safe water resources to fulfill increasing demands for agriculture. Here, we assess the efficiency of macrophyte Pistia stratiotes to remediate a polluted drain in Egypt, rich in macronutrients, heavy metals, and different types of pathogenic and non-pathogenic bacteria. Drainage water was sampled monthly, for a year, to assess seasonal changes in bacterial abundance, water physicochemical properties (transparency, temperature, dissolved oxygen, EC, pH, N, P, and K), and heavy metals contents (Pb, Zn, and Co) in a polluted drain dominated with P. stratiotes. The ability of P. stratiotes to rhizofiltrate the three heavy metals was calculated. The results showed seasonal variations in the plant rhizofiltration potential of Co and Salmonella abundance. The highest values of dissolved oxygen (12.36 mg/L) and macronutrient elements (N and P) were attained in the winter. The counts of total coliform, fecal coliform, fecal streptococci, and in Salmonella spp. were the highest in the summer. P. stratiotes accumulated Pb more than Zn and Co. The highest levels of rhizofiltration were in summer for Pb and Co and in the autumn for Zn. Canonical correspondence analysis (CCA) showed that the variation in the bacterial abundance and plant rhizofiltration potential was strongly and significantly affected by water-dissolved oxygen. Moreover, the rhizofiltration potential of Pb and Co showed a positive correlation with water N. Overall, P. stratiotes could be proposed as a potential biomonitor for heavy metals in polluted water.

Alleviation of Lead Stress on Sage Plant by 5-Aminolevulinic Acid (ALA).

Oxidative stress is imparted by a varying range of environmental factors involving heavy metal stress. Thus, the mechanisms of antioxidant resistance may advance a policy to improve metal tolerance. Lead as a toxic heavy metal negatively affects the metabolic activities and growth of medicinal and aromatic plants. This investigation aimed to assess the function of 5-aminolevulinic acid (ALA) in the alleviation of Pb stress in sage plants (Salvia officinalis L.) grown either hydroponically or in pots. Various concentrations of Pb (0, 100, 200, and 400 µM) and different concentrations of ALA (0, 10, and 20 mg L-1) were tested. This investigation showed that Pb altered the physiological parameters. Pb stress differentially reduced germination percentage and protein content compared to control plants. However, lead stress promoted malondialdehyde (MDA) and H2O2 contents in the treated plants. Also, lead stress enhanced the anti-oxidative enzyme activities; ascorbate peroxidase superoxide, dismutase, glutathione peroxidase, and glutathione reductase in Salvia plants. ALA application enhanced the germination percentage and protein content compared to their corresponding controls. Whereas, under ALA application MDA and H2O2 contents, as well as the activities of SOD, APX, GPX, and GR, were lowered. These findings suggest that ALA at the 20 mgL-1 level protects the Salvia plant from Pb stress. Therefore, the results recommend ALA application to alleviate Pb stress.

Waste-Derived NPK Nanofertilizer Enhances Growth and Productivity of Capsicum annuum L.

Waste generation is a global issue that necessitates effective management for both human and animal health as well as environment. There are several ways to reduce waste, but recycling appears to be the best choice. By recycling, not only will the problem of pollution be resolved, but valuable compounds could be generated to be used as nutrients for plants. In this study, eco-friendly methods were established to produce α- and ß-chitosan (CS) (as a source of nitrogen) with different degrees of deacetylation from shrimp shells and squid pin waste, phosphorous through degreasing and calcination of bovine bone and potassium from evaporation of banana peels Kolakhar. The waste bulk products were physically characterized and dry-milled into nano-powders. Different concentrations of the produced nano-NPK fertilizer (10%, 25%, 50% and 100%) were foliar-applied to Capsicum annum L. cv. Cordoba plants and compared to commercial chemical fertilizer and untreated control plants. The obtained results revealed that the nano-composite NPK with 25% concentration significantly promoted growth, yield and harvest of C. annuum as compared with the control and chemical fertilizer-treated plants. This study demonstrated that the use of an eco-friendly preparation of waste NPK composites, with a low concentration, could be applied as foliar fertilizer over chemical fertilizer to enhance the growth and productivity of Capsicum.

Phytoremediation Perspectives of Seven Aquatic Macrophytes for Removal of Heavy Metals from Polluted Drains in the Nile Delta of Egypt.

The current study addressed the heavy metals accumulation potentials of seven perennial aquatic macrophytes (Cyperus alopecuroides, Echinochloa stagnina, Eichhornia crassipes, Ludwigia stolonifera, Phragmites australis, Ranunculus sceleratus and Typha domingensis) and the pollution status of three drains (Amar, El-Westany and Omar-Beck) in the Nile Delta of Egypt. Nine sites at each drain were sampled for sediment and plant analyses. Concentrations of eight metals (Fe, Cu, Zn, Mn, Co, Cd, Ni, and Pb) were determined in the sediment and the aboveground and belowground tissues of the selected macrophytes. Bioaccumulation factor (BF) and translocation factor (TF) were computed for each species. The sediment heavy metals concentrations of the three drains occurred in the following order: El-Westany > Amar > Omar-Beck. The concentrations of sediment heavy metals in the three drains were ordered as follows: Fe (438.45-615.17 mg kg-1) > Mn (341.22-481.09 mg kg-1) > Zn (245.08-383.19 mg kg-1) > Cu (205.41-289.56 mg kg-1) > Pb (31.49-97.73 mg kg-1) > Cd (13.97-55.99 mg kg-1) > Ni (14.36-39.34 mg kg-1) > Co (1.25-3.51 mg kg-1). The sediment exceeded the worldwide permissible ranges of Cu, Zn and Pb, but ranged within safe limits for Mn, Cd, Ni and Co. P. australis accumulated the highest concentrations of Fe, Co, Cd and Ni, while E. crassipes contained the highest concentrations of Cu, Zn, Mn, and Pb. Except for C. alopecuroides and Cu metal, the studied species had BF values greater than one for the investigated heavy metals. Nevertheless, the TFs of all species (except Cd in L. stolonifera) were less than one. Hence, the studied species are appropriate for accumulation, biomonitoring, and phytostabilization of the investigated metals.

Structural and Chemical Adaptations of Artemisia monosperma Delile and Limbarda crithmoides (L.) Dumort. in Response to Arid Coastal Environments along the Mediterranean Coast of Egypt.

Arid coastal habitats are stressful regions subjected to the effects of biotic and abiotic factors. Vascular plants in these habitats display different responses to cope with these environmental fluctuations. This work addressed the morpho-anatomical features and chemical responses of two medicinal vascular plant species Artemisia monosperma Delile and Limbarda crithmoides (L.) Dumort., growing naturally along the Mediterranean coast of Egypt. Soil properties (physical and chemical), morpho-anatomical features and chemical constituents (secondary metabolites, antioxidant activity and essential oils) for the two species were performed. Our results displayed that both species are surviving where soils are alkaline, high saline with low moisture and organic carbon. The morphology of both species appeared woody low shrub with fleshy leaves. The most marked anatomical attributes were the thick cuticle of the epidermal layer in leaves and stems, compact palisade cells and abundant idioblasts (secretory ducts, phenolic compounds and calcium oxalate). Also, sclerenchymatous pericycle fibers in stem and glandular trichomes on the leaf had appeared in A. monosperma. Both plants exhibited a considerable content of phenolics, flavonoids, tannins, alkaloids and antioxidant activity with a higher level in A. monosperma than L. crithmoides. The leaf extracts of both plants showed higher values than the stem extracts. The sesquiterpenes group were the major identified compounds of the essential oils (EOs) in A. monosperma and L. crithmoides, and the majority were oxygenated sesquiterpenes with percentages of 42.63% and 51.49%, respectively. The second major group of EOs was monoterpenes, which were represented in A. monosperma in concentrations (34.04%) much higher than those recorded in L. crithmoides (4.97%). Exploring the local adaptation mechanism used by the target plants helps us to understand how these plants can acclimatize to harsh conditions, and this provides critical insights into the protection and survival strategy of species under extreme conditions.

Purification of soybean cupins and comparison of IgE binding with peanut allergens in a population of allergic subjects.

Identification, purification and characterization of allergens is crucial to the understanding of IgE-mediated disease. Immunologic and structural studies with purified allergens is essential for understanding relative immunogenicity and cross-reactivity. In this work, the complex soybean 7S vicilins (Gly m 5) with three subunits and 11S legumins (Gly m 6) with five subunits were purified and characterized along with purified peanut allergens (Ara h 1, 2, 3, and 6) by label-free liquid chromatography-tandem mass spectrometry (LC-MS/MS). Individual subjects plasma IgE binding was tested from subjects allergic to soybeans and or peanuts by immunoblotting, ImmunoCAP™ and ISAC™ ImmunoCAP chip, comparing these soybean proteins with those of purified peanut allergens; vicilin (Ara h 1), 2S albumin (Ara h 2 and Ara h 6) and 11S globulin (Ara h 3). Results show differences between methods and subjects demonstrating the complexity of finding answers to questions of cross-reactivity.

Predicting the Potential Current and Future Distribution of the Endangered Endemic Vascular Plant Primula boveana Decne. ex Duby in Egypt.

Knowledge about population attributes, current geographic distribution, and changes over predicted climate change for many threatened endemic vascular plants is particularly limited in arid mountain environments. Primula boveana is one of the rarest and threatened plants worldwide, surviving exclusively in Saint Catherine Protectorate in the Sinaic biogeographic subsector of Egypt. This study aimed to define the current state of P. boveana populations, predict its current potential distribution, and use the best-model outputs to guide in field sampling and to forecast its future distribution under two climate change scenarios. The MaxEnt algorithm was used by relating 10 occurrence-points with different environmental predictors (27 bioclimatic, 3 topographic, and 8 edaphic factors). At the current knowledge level, the population size of P. boveana consists of 796 individuals, including 137 matures, distributed in only 250 m2. The Canonical Correlation Analysis (CCorA) displayed that population attributes (density, cover, size index, and plant vigor) were positively correlated with elevation, precipitation, and pH. Based on the best-fitting model, most predicted suitable central sites (69 km2) of P. boveana were located in the cool shaded high-elevated middle northern part of St. Catherine. Elevation, precipitation, temperature, and soil pH were the key contributors to P. boveana distribution in Egypt. After field trips in suitable predicted sites, we confirmed five extinct localities where P. boveana has been previously recorded and no new population was found. The projected map showed an upward range shift through the contraction of sites between 1800 and 2000 m and expansion towards high elevation (above 2000 m) at the southern parts of the St. Catherine area. To conserve P. boveana, it is recommended to initiate in situ conservation through reinforcement and reintroduction actions.