RESUMO
No disponible
Assuntos
Humanos , Transtornos do Metabolismo dos Lipídeos/complicações , Hipersensibilidade Alimentar/fisiopatologia , Antígenos de Plantas/efeitos adversos , Índice de Gravidade de Doença , Alérgenos/isolamento & purificação , Testes Cutâneos/estatística & dados numéricos , Hipersensibilidade Respiratória/complicaçõesRESUMO
Our on-going goal is to improve and update the comparative genome organization between cattle and man but also among the most detailed mammalian species genomes i.e. cattle, mouse, rat and pig. In this work, we localized 195 genes in cattle and checked all human/bovine non-concordant localizations found in the literature. Next, we compiled all the genes mapped in cattle, goat, sheep and pig (2,166) for which the human ortholog with its chromosomal position is known, added corresponding data in mouse and rat, and ordered the genes relatively to the human genome sequence. We estimate that our compilation provides bovine mapping information for about 89% of the human autosomes. Thus, a near complete, overall and detailed picture of the number, distribution and extent of bovine conserved syntenies (regardless of gene order) on human R-banded autosomes is proposed as well as a comparison with mouse, rat and pig genomes.
Assuntos
Genes/genética , Mapeamento Físico do Cromossomo/veterinária , Animais , Bovinos , Cromossomos/genética , Cromossomos Humanos/genética , Cricetinae , Ordem dos Genes/genética , Humanos , Células Híbridas/química , Células Híbridas/metabolismo , Hibridização in Situ Fluorescente/métodos , Hibridização in Situ Fluorescente/veterinária , Camundongos , Ratos , Suínos , Sintenia/genéticaRESUMO
As part of the human genome study, large-scale cDNA sequencing has produced thousands of Expressed Sequence Tags (ESTs). Généthon has mapped in human 10,000 of these ESTs and has shown that the primers of about 1000 ESTs could amplify bovine DNA. In this work, we have analyzed 233 primer pairs provided by Genethon, to assign type I sequences to the bovine genome by using a hamster-bovine somatic cell hybrid panel. Among these 233 primer pairs, 109 gave a specific PCR product with bovine genomic DNA, but for 50% the size of the PCR product was the same in cattle and hamster, requiring SSCP analysis. Finally, 60 ESTs were assigned to the bovine genome, and among them 46 were found on the bovine chromosome expected from heterologous painting data between cattle and human.
Assuntos
Mapeamento Cromossômico , Etiquetas de Sequências Expressas , Animais , Bovinos , Cricetinae , DNA/química , DNA/genética , Primers do DNA , Feminino , Marcadores Genéticos , Humanos , Células Híbridas , Masculino , Dados de Sequência Molecular , Análise de Sequência de DNAAssuntos
Bovinos/genética , Fatores de Crescimento Neural/genética , Animais , Sequência de Bases , Galinhas , Bandeamento Cromossômico , Mapeamento Cromossômico , Primers do DNA/genética , Humanos , Hibridização in Situ Fluorescente , Camundongos , Dados de Sequência Molecular , Reação em Cadeia da Polimerase , Especificidade da Espécie , SuínosAssuntos
Bovinos/genética , Mapeamento Cromossômico , Animais , Feminino , Células Híbridas , Masculino , Polimorfismo GenéticoRESUMO
A cattle genetic linkage map was constructed which covers more than 95 percent of the bovine genome at medium density. Seven hundred and forty six DNA polymorphisms were genotyped in cattle families which comprise 347 individuals in full sibling pedigrees. Seven hundred and three of the loci are linked to at least one other locus. All linkage groups are assigned to chromosomes, and all are orientated with regards to the centromere. There is little overall difference in the lengths of the bull and cow linkage maps although there are individual differences between maps of chromosomes. One hundred and sixty polymorphisms are in or near genes, and the resultant genome-wide comparative analyses indicate that while there is greater conservation of synteny between cattle and humans compared with mice, the conservation of gene order between cattle and humans is much less than would be expected from the conservation of synteny. This map provides a basis for high-resolution mapping of the bovine genome with physical resources such as Yeast and Bacterial Artificial Chromosomes as well as providing the underpinning for the interpolation of information from the Human Genome Project.
Assuntos
Bovinos/genética , Mapeamento Cromossômico , Ligação Genética , Animais , Feminino , Humanos , Masculino , Camundongos , Polimorfismo GenéticoRESUMO
Bone morphogenetic protein 1 (BMP1) has been proposed as a regulatory molecule involved in the binding or activation of other BMP molecules. It has been described as being identical to the enzyme C-proteinase that processes procollagens to fibrillar collagens. A fragment containing an exon of the bovine BMP1 gene was amplified from an adapter-ligated genomic DNA library. The isolated bovine BMP1 fragment was assigned by fluorescence in situ hybridization (FISH) to bovine (BTA) chromosome 8q21, a region containing an evolutionary breakpoint between homology to human (HSA) chromosomes 8 and 9. The assignment to BTA 8 was confirmed by using a hybrid somatic cell panel. The 3' intron region of the amplified genomic BMP1 fragment showed a SSCP polymorphism, and linkage analysis in the International Bovine Reference Panel (IBRP) of families confirmed the boundary position of this gene. The BMP1 gene presents total linkage (theta = 0.00, Z = 3.61) with the lipoprotein lipase gene on BTA 8 and HSA 8 and a larger recombination fraction (theta = 0.11, Z = 5.03) with the marker GGTB2 on BTA 8 and HSA 9. Physical and genetic mapping of the bovine BMP1 gene contribute to narrow the boundary between the HSA 8 and HSA 9 homology segments on BTA 8 and to the comparative mapping between BTA 8, murine chromosome MMU 14, and HSA 8.
